scholarly journals Efficacy of different brands of penicillin-streptomycin against Staphylococcus aureus: the clinicians’ myths and the realities

2021 ◽  
Author(s):  
Takele Beyene Tufa ◽  
Asegid Guta ◽  
Tafese Beyene Tufa ◽  
Dereje Nigussie ◽  
Fikru Regassa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Animal Health ◽  
Dairy Farms ◽  
Cross Sectional Study ◽  
Cow Milk ◽  
Local Market ◽  
Efficacy Evaluation ◽  
Cross Sectional ◽  
Attitudes And Practices

Abstract Background Antibiotics must be safe, effective and have acceptable quality to use in both human and veterinary medicine. A cross-sectional study was conducted with the aim of evaluating in vitro efficacy of three brands of penicillin-streptomycin (penstrep) against Staphylococcus aureus isolated from dairy cow milk in Sebeta and Bishoftu, Ethiopia. A standardized questionnaire was also used to assess the knowledge, attitudes and practices (KAP) of veterinarians about brand antibiotics prescribing in dairy farms in the study area. Results A total of 43 S. aureus were isolated from 23 dairy farms. Thirty-three (81.4%) and 10 (23.3%) isolates were susceptible to brand A and intermediate susceptible to brand B, respectively, whereas only one isolate (2.3%) was intermediate susceptible to brand C. The isolates were also tested against the standard discs of penicillin and streptomycin, and the results showed that 42 (97.7%) and 27 (62.8%) of S. aureus were resistant, respectively. The KAP assessment showed penstrep was the most prescribed antibiotic in the dairy farms in the study area, followed by oxytetracycline and sulfa drugs. All veterinarians (100%, 30) agreed that antibiotics imported from Western countries perceived as having higher efficacy than those imported from eastern countries. Similarly, they preferred to use brand A, claiming better clinical improvements compared to the other two brands. The majority (86.7%) and more than half (53.3%) of the respondents agreed on the perception of overuse of antibiotics in veterinary clinics and dairy farms, respectively. Conclusions Penstrep Brand A has had better in vitro efficacy, while brands B and C have not been effective against S. aureus isolates. Moreover, this study revealed that a fixed combination dosage form of penicillin and streptomycin in brand A showed better efficacy towards S. aureus isolates compared to penicillin or streptomycin alone. The perception of the veterinarian about these brands agrees with the in-vitro antibacterial efficacy evaluation. The findings suggest that ineffective brands of penstrep are circulating in the local market, which constitute a potential danger to both human and animal health. Our findings warrant the need for stringent regulation and quality assessment criteria for imported veterinary antimicrobials.

In vitro efficacy of Daptomycin against clinical isolates of Methicillin-resistant Staphylococcus aureus (MRSA)

2020 ◽  
pp. 1-9
Author(s):  
Asim Ali Shah ◽  
Shahid Ahmad Abbasi ◽  
Yasir Ali ◽  
Ayesha Maqbool
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sampling Technique ◽  
Staining Technique ◽  
Cross Sectional Study ◽  
Clinical Isolates ◽  
Cross Sectional ◽  
Methicillin Resistant ◽  
Gram Staining

Abstract This descriptive cross-sectional study was performed in the Department of Microbiology, Fauji Foundation Hospital, Rawalpindi, from March 2019 to September 2019 to determine the in vitro efficacy of Daptomycin against clinical isolates of Methicillin-Resistant Staphylococcus aureus (MRSA). Consecutive non-probability sampling technique was used and a total number of 270 patients’ Pan Cultures having MRSA growth on Cefoxatin Disc with size less than 22 mm zone size were included in the study. Cultures were inoculated on MacConkey, Chocolate and Blood agar and then incubated for 24 hours at 37 degree Celsius. After incubation, Coagulase test, Catalase test and Gram staining technique were used for further identification. Minimum Inhibitory Concentration (MIC) of the isolates for Daptomycin was obtained by using E strips (Oxoid UK) according to Clinical & Laboratory Standards Institute (CLSI) guidelines. Continuous...  

scholarly journals Isolation and Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) from Milk in Shire Dairy Farms, Tigray, Ethiopia

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Weldemelak Girmay ◽  
Getachew Gugsa ◽  
Habtamu Taddele ◽  
Yisehak Tsegaye ◽  
Nesibu Awol ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cows ◽  
Bovine Mastitis ◽  
Pcr Amplification ◽  
Dairy Farms ◽  
Cross Sectional Study ◽  
Penicillin G ◽  
Isolation And Identification ◽  
Cross Sectional ◽  
California Mastitis Test

Antibiotic-resistant Staphylococcus aureus isolates pose a severe challenge to both veterinary and health professions and dairy cattle producers. Cross-sectional study was conducted from November 2014 to May 2015 to isolate and identify S. aureus from mastitic cows’ milk and estimate the occurrence of MRSA in the dairy farms of Shire. Physical examination and California mastitis test were performed on a total of 220 dairy cows. Bacteriological isolation and identification and antibiogram testing were performed. Furthermore, multiplex polymerase chain reaction (PCR) was done for the detection of mec A and fem A genes. Out of the 220 dairy cows, 64 (29.09%) were positive for bovine mastitis, and of these, 32.81% were coagulase-positive S. aureus (CoPS). Antibiogram test results showed 100% of the isolates were resistant to penicillin G, nalidixic acid, and ampicillin, and 33.33% of the CoPS showed resistance to oxacillin (phenotypically MRSA positive). But 38.09% of the CoPS were found to be resistant and susceptible to vancomycin. PCR amplification of the seven phenotypically MRSA isolates revealed that 42.9% and 71.4% of them were found to carry fem A and mec A genes, respectively. The current study revealed the existence of alarming level of CoPS and development of multidrug resistance.

Mode d’utilisation des antibiotiques dans les élevages de bovin laitier sédentaires au nord-ouest du Bénin

2019 ◽  
Vol 42.2 ◽  
pp. 7198-7206
Author(s):  
Mensah Serge Egide Paulin ◽  
Sessou Philippe ◽  
Nata Christie ◽  
Adjahoutonon Koomlan Yélindo Kadjinou Brice ◽  
Lahamy Olivier ◽  
...  
Keyword(s):  
Antibiotic Treatment ◽  
Dairy Farms ◽  
Cow Milk ◽  
Veterinary Services ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Antibiotics Residues ◽  
Lait De Vache

Une enquête transversale a été faite pour évaluer l’utilisation des antibiotiques dans les élevages de production de lait de vache des communes de Natitingou, Kérou, Pehunco, Kouandé et Djougou au nord-ouest du Bénin. Sur 452 éleveurs enquêtés, 347 (76,7%) utilisaient des antibiotiques sans assistance vétérinaire pour le traitement des affections respiratoires (19,0%), les entérites néo natales (19,0%), les dermatoses (18,0%), les plaies (17,0%), les mammites (17,0%) ou d’autres maladies (10,0%). Les tétracyclines (100,0%), les pénicillines (100,0%), les sulfamides (47,1%) et les macrolides (16,8%) étaient les familles d’antibiotiques les plus utilisées. 35,6% des éleveurs achetait les antibiotiques au marché contre 84,4% chez un vétérinaire et 35,1% les achetait sans le conseil d’un vétérinaire. Plus de 50% des éleveurs ne consulte pas un vétérinaire pour l’administration des antibiotiques ou n’adapte jamais la posologie selon le poids des bovins traités. Enfin, 83,6% des éleveurs faisaient traire les vaches sous traitement antibiotiques tout comme les vaches sans traitement. Les services vétérinaires du Bénin devraient mieux contrôler de l’utilisation des antibiotiques en production de lait de vache et sensibiliser les éleveurs sur le risque représenté par la présence de résidus d’antibiotiques dans le lait de vache consommé. ABSTRACT A cross sectional survey was conducted to assess the use of antibiotic in dairy farms in the municipalities of Natitingou, Kérou, Pehunco, Kouandé and Djougou in northwestern Benin. Three hundred and Forty seven 347 (76.7%) of the 452 surveyed farmers used antibiotics without veterinary assistance against neonatal enteritis (19.0%), dermatitis (18.0%), wounds (17.0%), mastitis (17.0%) or others diseases (10.0%). Tetracyclines (100.0%), penicillins (100.0%), sulfonamides (47.1%) and macrolides (16.8 %) and were the most used families of antibiotics. 35.6% of breeders bought antibiotics at the market against 84.4% at a veterinarian and 35.1% bought them without the advice of a veterinarian. More than 50% of breeders do not consult a veterinarian for the administration of antibiotics or never adapt the dosage according to the weight of the treated cattle. Finally, 83.6% of breeders milked cows under antibiotic treatment as cows without treatment. Benin veterinary services should more control the use of antibiotics in dairy farms and inform the farmer about the risk associated with the presence of antibiotics residues in consumed cow milk.

Suppurative Infections in Hospitalized Patients – An Ongoing MRSA Threat

2020 ◽  
Author(s):  
Sadaf Razzak
Keyword(s):  
Staphylococcus Aureus ◽  
Medical Science ◽  
Great Influence ◽  
Antibiotic Sensitivity ◽  
Skin Lesions ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Inducible Clindamycin Resistance ◽  
Empiric Antibiotic ◽  
Medical Wards

Background: Penicillin resistance among Staphylococcus aureus commonly encountered in the hospital admitted patients. Detection of antibiotic sensitivity in hospital acquired methicillin resistant Staphylococcus aureus infections is important as it has great influence on empiric antibiotic prescription, successful control of infection, prevention of spread of disease and successful patient management. This study aimed to detect the frequency of HA-MRSA from pus samples in a hospital setup with assessment of their antibiotic susceptibility patterns. Method: A cross-sectional study was conducted in the Microbiology department of Basic Medical Science Institute, JPMC, Karachi from January 2015 until December 2015. Pus samples from surgical site wounds, skin lesions, abscesses from surgical and medical wards and ICUs were collected. According to the standards given by CLSI 2014, MRSA testing of the samples was done and susceptibility testing for antibiotics was performed. Inducible clindamycin resistance was detected by D-Test; E Test. determined MIC (minimum inhibitory concentration) for vancomycin. The data was analyzed by SPSS version 16. Result: Out of the 149 MRSA identified from the pus samples, 106 (71.14%) samples were HA-MRSA. The number of male patients was more than the female patients (67.66%). Out of the 106 HA-MRSA, 91(85.8%) were sensitive to TMP/SMX, 98(92.5%) to rifampicin, 12(11.6%) to gentamicin, 85(80.2%) to tetracycline, 11(10.4%) to erythromycin, 37(34.9%) to clindamycin, 20(18.9%) to ciprofloxacin, 106 (100%) to both vancomycin and linezolid. Conclusion: HA-MRSA showed sensitivity to TMP/SMX and vancomycin making them effective drugs to use in combination in superficial infections. The drug linezolid also showed 100% sensitivity.

scholarly journals Knowledge, attitudes, and practices of Cameroonian physicians with regards to acute pain management in the emergency department: a multicenter cross-sectional study

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Paul Owono Etoundi ◽  
Junette Arlette Metogo Mbengono ◽  
Ferdinand Ndom Ntock ◽  
Joel Noutakdie Tochie ◽  
Dominique Christelle Anaba Ndom ◽  
...  
Keyword(s):  
Emergency Department ◽  
Pain Management ◽  
Acute Pain ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Acute Pain Management ◽  
Cross Sectional ◽  
Attitudes And Practices ◽  
Knowledge Attitudes And Practices

scholarly journals Unravelling the Perspectives of Day and Night Traders in Selected Markets within a Sub-Saharan African City with a Malaria Knowledge, Attitude and Practice Survey

2021 ◽  
Vol 18 (7) ◽  
pp. 3468
Author(s):  
Patience B. Tetteh-Quarcoo ◽  
Nicholas T. K. D. Dayie ◽  
Kevin Kofi Adutwum-Ofosu ◽  
John Ahenkorah ◽  
Emmanuel Afutu ◽  
...  
Keyword(s):  
Cross Sectional Study ◽  
Malaria Treatment ◽  
Sub Saharan Africa ◽  
African City ◽  
Cross Sectional ◽  
Attitudes And Practices ◽  
Treatment Type ◽  
Significant Difference ◽  
High Knowledge ◽  
Sub Saharan

Background: Malaria is still endemic in sub-Saharan Africa, with a high disease burden. Misconceptions about malaria contribute to poor attitudes and practices, further increasing the burden in endemic countries. Studies have examined the knowledge, attitudes, and practices (KAP) of malaria among different populations. However, there seems to be no available literature reporting on the perspectives of day and night market traders. To the best of our knowledge, this is the first report on malaria KAP with a focus on day and night market traders. Methods: A descriptive cross-sectional study involving day and night market traders in 10 selected markets within the Greater Accra Region of Ghana was carried out. Data were collected from consenting respondents using a structured questionnaire. Results: Of the 760 respondents (33.3% (n = 253) night and 66.7% (n = 507) day traders) interviewed, there was no significant difference between the day and night market traders in terms of malaria KAP. Although the market traders had an overall moderate knowledge (54.0% of the day traders and 56.5% of the night traders), misconceptions about malaria (especially that it could be caused by exposure to the sun) still existed among the traders. Moreover, the majority of the traders who demonstrated high knowledge (43.98%, n = 250) did not always take laboratory tests to confirm their suspicion, indicating poor attitude. Furthermore, the market traders’ choice of drug for malaria treatment (p = 0.001) and preferred malaria treatment type (orthodox or herbal) (p = 0.005) were significantly associated with their knowledge level. Conclusions: Despite the observation that no significant difference in KAP exists between day and night market traders, appropriate health education programs and interventions still need to be directed at misconceptions, poor attitudes, and poor practices revealed by this study. This will ultimately help in the prevention and control of malaria in Ghana, and globally.

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