Optimizing Isobutanol Production in Engineered Cyanobacteria Using High Salinity Stress and Establishment of a Resource Recycling Strategy

Author(s):  
Xiao-Xi Wu ◽  
Jian-Wei Li ◽  
Su-Fang Xing ◽  
Hui-Ting Chen ◽  
Chao Song ◽  
...  

Abstract Background: Isobutanol is an attractive biofuel with advantages. Third-generation biorefineries that convert CO2 into bio-based fuels have drawn considerable attention due to their lower feedstock cost and more ecofriendly refining process. Although autotrophic cyanobacteria have been genetically modified for isobutanol biosynthesis, it is still lack of stable and convenient strategies to improve the production.Results: In this study, we first engineered Synechococcus elongatus for isobutanol biosynthesis by introducing five exogenous enzymes, reaching a production titer of 0.126 g/L at day 20. It was then discovered that high salinity stress can significantly enhance isobutanol production five-fold with a maximal in-flask titer of 0.637 g/L at day 20. A comprehensive analysis of the osmotic-induced cells demonstrated that a series of physiological functional self-adjustment in S. elongatus, including altering metabolic profiles to accumulate redox equivalents, strength in antioxidative ability and weaken in membrane integrity, contributed to the isobutanol production titer. A cultivation system was then developed by mixing wastewater with seawater to grow the engineered cyanobacteria, reaching a similar isobutanol production titer as cultivation in the medium. Conclusions: High salinity stress on engineered cyanobacteria is a practical and feasible biotechnology to optimize isobutanol production. This biotechnology provides a prospect for biofuel production cost-effectively, and simultaneously recycles chemical nutrients from wastewater and seawater.

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Xiao-Xi Wu ◽  
Jian-Wei Li ◽  
Su-Fang Xing ◽  
Hui-Ting Chen ◽  
Chao Song ◽  
...  

Abstract Background Isobutanol is an attractive biofuel with many advantages. Third-generation biorefineries that convert CO2 into bio-based fuels have drawn considerable attention due to their lower feedstock cost and more ecofriendly refining process. Although autotrophic cyanobacteria have been genetically modified for isobutanol biosynthesis, there is a lack of stable and convenient strategies to improve their production. Results In this study, we first engineered Synechococcus elongatus for isobutanol biosynthesis by introducing five exogenous enzymes, reaching a production titer of 0.126 g/L at day 20. It was then discovered that high salinity stress could result in a whopping fivefold increase in isobutanol production, with a maximal in-flask titer of 0.637 g/L at day 20. Metabolomics analysis revealed that high salinity stress substantially altered the metabolic profiles of the engineered S. elongatus. A major reason for the enhanced isobutanol production is the acceleration of lipid degradation under high salinity stress, which increases NADH. The NADH then participates in the engineered isobutanol-producing pathway. In addition, increased membrane permeability also contributed to the isobutanol production titer. A cultivation system was subsequently developed by mixing synthetic wastewater with seawater to grow the engineered cyanobacteria, reaching a similar isobutanol production titer as cultivation in the medium. Conclusions High salinity stress on engineered cyanobacteria is a practical and feasible biotechnology to optimize isobutanol production. This biotechnology provides a cost-effective approach to biofuel production, and simultaneously recycles chemical nutrients from wastewater and seawater.


2018 ◽  
Vol 56 (1) ◽  
pp. 56-64 ◽  
Author(s):  
Ramesh Kakarla ◽  
Jung-Woon Choi ◽  
Jin-Ho Yun ◽  
Byung-Hyuk Kim ◽  
Jina Heo ◽  
...  

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Akihiro Matsui ◽  
Kayoko Mizunashi ◽  
Maho Tanaka ◽  
Eli Kaminuma ◽  
Anh Hai Nguyen ◽  
...  

In plants, miRNAs and siRNAs, such as transacting siRNAs (ta-siRNAs), affect their targets through distinct regulatory mechanisms. In this study, the expression profiles of small RNAs (smRNAs) inArabidopsisplants subjected to drought, cold, and high-salinity stress were analyzed using 454 DNA sequencing technology. Expression of three groups of ta-siRNAs (TAS1, TAS2, and TAS3) and their precursors was downregulated inArabidopsisplants subjected to drought and high-salinity stress. Analysis of ta-siRNA synthesis mutants and mutatedARF3-overexpressing plants that escape the tasiRNA-ARF target indicated that self-pollination was hampered by short stamens in plants under drought and high-salinity stress. Microarray analysis of flower buds ofrdr6and wild-type plants under drought stress and nonstressed conditions revealed that expression of floral development- and auxin response-related genes was affected by drought stress and by theRDR6mutation. The overall results of the present study indicated that tasiRNA-ARF is involved in maintaining the normal morphogenesis of flowers in plants under stress conditions through fine-tuning expression changes of floral development-related and auxin response-related genes.


2020 ◽  
Vol 18 ◽  
pp. 100423
Author(s):  
Chao Li ◽  
Na Li ◽  
Tiantian Dong ◽  
Qiang Fu ◽  
Yanting Cui ◽  
...  

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