Salt Stress-Induced Genetic Variation in Calli and Shoot Cultures of Economically Important Stevia Rebaudiana

2021 ◽  
Author(s):  
Hayat Khan ◽  
Hina Qasim ◽  
Zarghoona Zahoor ◽  
Nisar Ahmad ◽  
Arshad Iqbal ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Salt Stress ◽  
Leaf Explants ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Shoot Cultures ◽  
Stem Cuttings ◽  
Market Demand ◽  
Leaf Sample ◽  
Homogeneous Population

Abstract Stevia rebaudiana is one of the potent members of family Asteraceae and is famous for the synthesis of Steviol glycosides. Recently it is one of the economically important plants with high market demand. Seed infertility and stem cuttings are the major issues in obtaining homogeneous population, therefore, the best alternative is plant cell, tissue and organ culture. Currently, salt stress is one of the major issues worldwide. The overall objective of the current study was to investigate the effect of salt stress (100 mm NaCl) on genetic variation in calli and shoot cultures of S. rebaudiana. Sterilized leaf explants were inoculated on MS media augmented with 2,4-D (2.0 mg/L) and BAP (2.0 mg/L) for callogenesis. Shoots were obtained by using BAP (2.0 mg/L) and GA3 (0.5 mg/L) after 30 days. Callus and shoots were exposed to salt stress for 30 days. These fresh calli and shoot cultures were analyzed for genetic variation including three genes. 1: Leaf sample UDP glycosyltransferase gene restricted with ScaI 2: Callus sample gene restricted with ScaI 3: Leaf sample UDP glycosyltransferase mRNA restricted with NcoI 4: Callus sample mRNA restricted with NcoI 5: Leaf sample UDP glycosyltransferase mRNA restricted with DraIII 6: Callus sample mRNA restricted with DraIII. Only the callus sample digested with DraIII showed a variation from the mother plant. Others genes did not show any variation. It is concluded that genetic variation occurs in callus which is undifferentiated mass of cells and the variation caused may be due to salt stress. This study further need sequencing to identify the genes responsible for genetic and Somaclonal variation.

scholarly journals Salt Stress Mitigation via the Foliar Application of Chitosan-Functionalized Selenium and Anatase Titanium Dioxide Nanoparticles in Stevia (Stevia rebaudiana Bertoni)

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4090
Author(s):  
Morteza Sheikhalipour ◽  
Behrooz Esmaielpour ◽  
Gholamreza Gohari ◽  
Maryam Haghighi ◽  
Hessam Jafari ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Salt Stress ◽  
Titanium Dioxide Nanoparticles ◽  
Chitosan Nanoparticles ◽  
Stevia Rebaudiana ◽  
High Salt ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Tio2 Nps ◽  
Stevia Rebaudiana Bertoni

High salt levels are one of the significant and major limiting factors on crop yield and productivity. Out of the available attempts made against high salt levels, engineered nanoparticles (NPs) have been widely employed and considered as effective strategies in this regard. Of these NPs, titanium dioxide nanoparticles (TiO2 NPs) and selenium functionalized using chitosan nanoparticles (Cs–Se NPs) were applied for a quite number of plants, but their potential roles for alleviating the adverse effects of salinity on stevia remains unclear. Stevia (Stevia rebaudiana Bertoni) is one of the reputed medicinal plants due to their diterpenoid steviol glycosides (stevioside and rebaudioside A). For this reason, the current study was designed to investigate the potential of TiO2 NPs (0, 100 and 200 mg L−1) and Cs–Se NPs (0, 10 and 20 mg L−1) to alleviate salt stress (0, 50 and 100 mM NaCl) in stevia. The findings of the study revealed that salinity decreased the growth and photosynthetic traits but resulted in substantial cell damage through increasing H2O2 and MDA content, as well as electrolyte leakage (EL). However, the application of TiO2 NPs (100 mg L−1) and Cs–Se NPs (20 mg L−1) increased the growth, photosynthetic performance and activity of antioxidant enzymes, and decreased the contents of H2O2, MDA and EL under the saline conditions. In addition to the enhanced growth and physiological performance of the plant, the essential oil content was also increased with the treatments of TiO2 (100 mg L−1) and Cs–Se NPs (20 mg L−1). In addition, the tested NPs treatments increased the concentration of stevioside (in the non-saline condition and under salinity stress) and rebaudioside A (under the salinity conditions) in stevia plants. Overall, the current findings suggest that especially 100 mg L−1 TiO2 NPs and 20 mg L−1 Cs–Se could be considered as promising agents in combating high levels of salinity in the case of stevia.

Biotechnological Production of Natural Calorie Free Steviol Glycosides in Stevia rebaudiana: An Update on Current Scenario

2020 ◽  
Vol 8 (2) ◽  
pp. 70-84
Author(s):  
Abeer Kazmi ◽  
Mubarak Ali Khan ◽  
Sher Mohammad ◽  
Amir Ali ◽  
Huma Ali
Keyword(s):  
Medicinal Plant ◽  
Cell Cultures ◽  
Metabolic Pathways ◽  
Plant Material ◽  
Research Work ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Market Demand ◽  
Biotechnological Production ◽  
Current Scenario

Stevia rebaudiana is a vital medicinal plant of the genus Stevia and family Asteraceae. It is commonly used as a natural sweetener plant and its products are 300 times sweeter than the commonly used sugarcane. The sweetening potential is due to the presence of calorie-free steviol glycosides (SGs). The plant species has been extensively profiled to identify steviol glycosides (SGs) with intensity sweetening properties. However, the limited production of plant material is not fulfilling the higher market demand worldwide. Researchers are working worldwide to enhance the production of important SGs through the intervention of different biotechnological approaches in S. rebaudiana. In this review, the research work conducted in the last twenty years, on the different aspects of biotechnology to enhance the production of SGs has been precisely reviewed. Biotechnological methods such as micropropagation, callus and cell cultures, elicitation and the metabolomics and transcriptomic elucidation of the biosynthetic metabolic pathways for the production of steviol glycosides have been concisely reviewed and discussed.

Salt Stress-Induced Changes in In Vitro Cultured Stevia rebaudiana Bertoni: Effect on Metabolite Contents, Antioxidant Capacity and Expression of Steviol Glycosides-Related Biosynthetic Genes

2019 ◽  
Vol 38 (4) ◽  
pp. 1341-1353 ◽  
Author(s):  
Simone Ribeiro Lucho ◽  
Marcelo Nogueira do Amaral ◽  
Priscila Ariane Auler ◽  
Valmor João Bianchi ◽  
María Ángeles Ferrer ◽  
...  
Keyword(s):  
Salt Stress ◽  
Antioxidant Capacity ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Biosynthetic Genes ◽  
Stevia Rebaudiana Bertoni ◽  
Induced Changes

scholarly journals Characterization of Stevia rebaudiana Bertoni Accessions Cultived in Southern Brazil

2018 ◽  
Vol 10 (3) ◽  
pp. 353 ◽  
Author(s):  
Felipe Francisco ◽  
Gabriely Pinto Pereira ◽  
Marília Pereira Machado ◽  
Luiz Alberto Kanis ◽  
Cícero Deschamps
Keyword(s):  
Native Species ◽  
Crop Improvement ◽  
Stevia Rebaudiana ◽  
Biomass Accumulation ◽  
Steviol Glycosides ◽  
Market Demand ◽  
Southern Brazil ◽  
Stevia Rebaudiana Bertoni ◽  
Commercially Important

Stevia, a native species of South America, is commercially important as a source of the non-caloric diterpene glycosides stevioside and rebaudioside-A. Although it has been cultivated in southern Brazil, the production is not enough to meet the domestic market demand. This study evaluated biomass accumulation, morphological and physiological characters, and steviol glycosides yield of seven stevia accessions after two cuts. A great variation for the different plant characteristics was found between the first and second cuts. There was a positive correlation between biomass production and SGs content and productivity. The overall Principal Components Analysis results in both cuts indicate that the accessions CE7 and CE14 presented great Reb-A yield, height and lodging (%). The accessions CE4 and CE12 produced high Ste yield and accessions CE6, CE8 and CE9 the highest leaf:stem ratio and low SGs. The stevia accessions showed characteristics of the interest for crop improvement for both biomass and SGs production.

scholarly journals Effect of Growth Regulators on Stevia rebaudiana Bertoni Callus Genesis and Influence of Auxin and Proline to Steviol Glycosides, Phenols, Flavonoids Accumulation, and Antioxidant Activity In Vitro

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2759
Author(s):  
Aušra Blinstrubienė ◽  
Natalija Burbulis ◽  
Neringa Juškevičiūtė ◽  
Nijolė Vaitkevičienė ◽  
Rasa Žūkienė
Keyword(s):  
Antioxidant Activity ◽  
Growth Regulators ◽  
Callus Induction ◽  
Leaf Explants ◽  
Total Phenolic ◽  
Naphthalene Acetic Acid ◽  
Steviol Glycosides ◽  
Ms Medium ◽  
Stem Cuttings ◽  
The Impact

Stevia is a plant containing many active compounds, but usually propagated by stem cuttings because of low seed-yield-germination ability. The aim of this study was to investigate the impact of plant-growth regulators on stevia callus induction and growth from somatic tissue, as well as to determine the effect α-naphthalene acetic acid (NAA) and proline (PRO) on the amount of stevioside, rebaudioside A, phenols, flavonoids, and antioxidant activity. Stem and leaf segments were inoculated on a Murashige and Skoog (MS) medium supplemented with different concentrations of NAA and 6-benzylaminopurine (BAP) for callus genesis. The amount of steviol glycosides (SGs) was evaluated using high-performance liquid chromatography (HPLC), and the amounts of total phenols, flavonoids, and antioxidant activity by spectrophotometric methods. The highest callus-induction frequency and callus-mass increase were obtained from the leaf explants in MS medium supplemented with 2.0 μM NAA. The highest amount of SGs, phenols, and flavonoids, and stronger antioxidant activity were determined in the cellular compounds of callus from leaf explant. PRO reduced the amount of SGs and flavonoids. The significantly highest amount of total phenolic compounds was obtained in the callus from leaf explants in the medium supplemented with 2.0 µM NAA and 2.0 µM PRO.

Methanol elicits the accumulation of bioactive steviol glycosides and phenolics in Stevia rebaudiana shoot cultures

2016 ◽  
Vol 87 ◽  
pp. 273-279 ◽  
Author(s):  
María José Álvarez-Robles ◽  
Antonio López-Orenes ◽  
María A. Ferrer ◽  
Antonio A. Calderón
Keyword(s):  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Shoot Cultures

scholarly journals Comparative Assessment of the Basic Chemical Composition and Antioxidant Activity of Stevia rebaudiana Bertoni Dried Leaves, Grown in Poland, Paraguay and Brazil—Preliminary Results

2021 ◽  
Vol 11 (8) ◽  
pp. 3634
Author(s):  
Teresa Leszczyńska ◽  
Barbara Piekło ◽  
Aneta Kopeć ◽  
Benno F. Zimmermann
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Vitamin C ◽  
Dietary Fiber ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Total Polyphenols ◽  
Stevia Rebaudiana Bertoni

This study compares the content of basic nutrients (proteins, fats, digestible carbohydrates, dietary fiber and ash), steviol glycosides, selected antioxidants (vitamin C, total polyphenols) and antioxidant activity in dried leaves of Stevia rebaudiana Bertoni cultivated in Poland, Paraguay and Brazil and available in the direct sale. The basic chemical composition was determined by standard AOAC (Association of Official Analytical Chemists) methods. Content of steviol glycosides was determined by the UHPLC-UV chromatographic method. Total polyphenols content was expressed as gallic acid equivalent (GAE) and catechins equivalent (CE). Antioxidant activity was measured as ABTS●+ free radical scavenging activity. Dried leaves of S. rebaudiana grown in Poland had significantly higher contents of dietary fiber, and lower protein and ash content, compared to those derived from Paraguay and Brazil. The former had, however, considerably higher contents of total steviol glycosides, stevioside and rebaudioside D, compared to the remaining two plants. In the Paraguay-derived dried leaves, the content of rebaudioside A, C, E and rubusoside was found to be significantly lower. Dried leaves of S. rebaudiana Bertoni, cultivated in Poland, contained substantially more vitamin C and a similar content of total polyphenols, compared to those from Brazil and Paraguay. The examined material from Brazil and Paraguay plantations showed similar antioxidant activity, while that obtained from Polish cultivation was characterized by a significantly lower value of this parameter.

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