scholarly journals GhCIPK6 Increases Salt Tolerance in Transgenic Upland Cotton by Involving in ROS Scavenging and MAPK Signaling Pathways

2020 ◽  
Author(s):  
Ying Su ◽  
Anhui Guo ◽  
Yi Huang ◽  
Yumei Wang ◽  
Jinping Hua
Keyword(s):  
Salt Stress ◽  
Cell Membrane ◽  
Signaling Pathways ◽  
Abiotic Stresses ◽  
Upland Cotton ◽  
Mapk Signaling ◽  
Control Line ◽  
Rna Seq ◽  
Ros Scavenging ◽  
Mapk Signaling Pathways

Abstract Background: Salt stress is one of the most damaging abiotic stresses in production of Upland cotton ( Gossypium hirsutum ). Upland cotton is defined as a medium salt-tolerant crop. Salinity hinders root development, shoots growth, and reduces the fiber quality. Results: Our previous study verified a GhCIPK6 gene response to salt stress in G. hirsutum . The homologs of GhCIPK6 were analyzed in A 2 ( G. arboreum ), D 5 ( G . raimondii ), and AD 1 ( G . hirsutum ) genomes. GhCIPK6 localized to the vacuole and cell membrane. The GhCBL1-GhCIPK6 and GhCBL8-GhCIPK6 complexes localized to the nucleus and cytomembrane. Overexpression of GhCIPK6 enhanced expression levels of co-expressed genes induced by salt stress, which scavenged ROS and involved in MAPK signaling pathways verified by RNA-seq analysis. Water absorption capacity and cell membrane stability of seeds from GhCIPK6 overexpressed lines was higher than that of wild-type seeds during imbibed germination stage. The seed germination rates and seedling field emergence percentages of GhCIPK6 overexpressed lines were higher than that of control line under salt stress. Moreover, overexpressing of GhCIPK6 in cotton increased lint percentage, and fiber length uniformity under salt stress. Conclusions: We verified the function of GhCIPK6 by transformation and RNA-seq analysis. GhCIPK6 overexpressed lines exhibited higher tolerance to abiotic stresses, which functioned by involving in ROS scavenging and MAPK pathways. Therefore, GhCIPK6 has the potential for cotton breeding to improve stress-tolerance.

scholarly journals GhCIPK6a increases salt tolerance in transgenic upland cotton by involving in ROS scavenging and MAPK signaling pathways

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Ying Su ◽  
Anhui Guo ◽  
Yi Huang ◽  
Yumei Wang ◽  
Jinping Hua
Keyword(s):  
Salt Stress ◽  
Cell Membrane ◽  
Signaling Pathways ◽  
Abiotic Stresses ◽  
Upland Cotton ◽  
Mapk Signaling ◽  
Control Line ◽  
Rna Seq ◽  
Ros Scavenging ◽  
Mapk Signaling Pathways

Abstract Background Salt stress is one of the most damaging abiotic stresses in production of Upland cotton (Gossypium hirsutum). Upland cotton is defined as a medium salt-tolerant crop. Salinity hinders root development, shoots growth, and reduces the fiber quality. Results Our previous study verified a GhCIPK6a gene response to salt stress in G. hirsutum. The homologs of GhCIPK6a were analyzed in A2 (G. arboreum), D5 (G. raimondii), and AD1 (G. hirsutum) genomes. GhCIPK6a localized to the vacuole and cell membrane. The GhCBL1-GhCIPK6a and GhCBL8-GhCIPK6a complexes localized to the nucleus and cytomembrane. Overexpression of GhCIPK6a enhanced expression levels of co-expressed genes induced by salt stress, which scavenged ROS and involved in MAPK signaling pathways verified by RNA-seq analysis. Water absorption capacity and cell membrane stability of seeds from GhCIPK6a overexpressed lines was higher than that of wild-type seeds during imbibed germination stage. The seed germination rates and seedling field emergence percentages of GhCIPK6a overexpressed lines were higher than that of control line under salt stress. Moreover, overexpressing of GhCIPK6a in cotton increased lint percentage, and fiber length uniformity under salt stress. Conclusions We verified the function of GhCIPK6a by transformation and RNA-seq analysis. GhCIPK6a overexpressed lines exhibited higher tolerance to abiotic stresses, which functioned by involving in ROS scavenging and MAPK pathways. Therefore, GhCIPK6a has the potential for cotton breeding to improve stress-tolerance.

scholarly journals A CBL-interacting Protein Kinase Gene, GhCIPK6, Increases Salt Tolerance in Transgenic Upland Cotton by Involving in Multiple Stress Signaling Pathways

2020 ◽  
Author(s):  
Ying Su ◽  
Anhui Guo ◽  
Yi Huang ◽  
Yumei Wang ◽  
Jinping Hua
Keyword(s):  
Salt Stress ◽  
Cell Membrane ◽  
Signaling Pathways ◽  
Abiotic Stresses ◽  
Upland Cotton ◽  
Fiber Quality ◽  
Control Line ◽  
Rna Seq ◽  
Kinase Gene ◽  
Multiple Stress

Abstract Background: Salt stress is one of the most damaging abiotic stresses in production of Upland cotton (Gossypium hirsutum). Upland cotton is defined as a medium salt-tolerant crop. Salinity hinders root development, shoots growth, and reduces the fiber quality.Results: Our previous study verified a GhCIPK6 gene response to salt stress in G. hirsutum. The homologs of GhCIPK6 were analyzed in A2 (G. arboreum), D5 (G. raimondii), and AD1 (G. hirsutum) genomes. GhCIPK6 localized to the vacuole and cell membrane. The GhCBL1-GhCIPK6 and GhCBL8-GhCIPK6 complexes localized to the nucleus and cytomembrane. Overexpression of GhCIPK6 enhanced expression levels of co-expressed genes induced by salt stress, which scavenged ROS and involved in multiple signaling pathways verified by RNA-seq analysis. Water absorption capacity and cell membrane stability of seeds from GhCIPK6 overexpressed lines was higher than that of wild-type seeds during imbibed germination stage. The germination rates and field emergence percentage of GhCIPK6 overexpressed lines were higher than that of control line under salt stress. Moreover, overexpressing of GhCIPK6 in cotton increased lint percentage, and fiber length uniformity under salt stress. Conclusions: We verified the function of GhCIPK6 by transformation and RNA-seq analysis. GhCIPK6 overexpressed lines exhibited higher tolerance to abiotic stresses, which functioned by involving in multiple stress responsive pathways. Therefore, GhCIPK6 has the potential for cotton breeding to improve stress-tolerance.

scholarly journals Urolithin A attenuates renal fibrosis by inhibiting TGF-β1/Smad and MAPK signaling pathways

2021 ◽  
Vol 83 ◽  
pp. 104547
Author(s):  
Zhenzhen Cheng ◽  
Jingjing Tu ◽  
Hongpan Zhang ◽  
Yi zhang ◽  
Benhong Zhou
Keyword(s):  
Signaling Pathways ◽  
Renal Fibrosis ◽  
Mapk Signaling ◽  
Urolithin A ◽  
Mapk Signaling Pathways ◽  
Tgf Β1

scholarly journals Down-Regulation of SlGRAS10 in Tomato Confers Abiotic Stress Tolerance

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 623
Author(s):  
Sidra Habib ◽  
Yee Yee Lwin ◽  
Ning Li
Keyword(s):  
Salt Stress ◽  
Abiotic Stress ◽  
Plant Growth ◽  
Stress Tolerance ◽  
Abiotic Stresses ◽  
Abiotic Stress Tolerance ◽  
Functional Characterization ◽  
Flavonoid Biosynthesis ◽  
Ros Scavenging ◽  
Down Regulation

Adverse environmental factors like salt stress, drought, and extreme temperatures, cause damage to plant growth, development, and crop yield. GRAS transcription factors (TFs) have numerous functions in biological processes. Some studies have reported that the GRAS protein family plays significant functions in plant growth and development under abiotic stresses. In this study, we demonstrated the functional characterization of a tomato SlGRAS10 gene under abiotic stresses such as salt stress and drought. Down-regulation of SlGRAS10 by RNA interference (RNAi) produced dwarf plants with smaller leaves, internode lengths, and enhanced flavonoid accumulation. We studied the effects of abiotic stresses on RNAi and wild-type (WT) plants. Moreover, SlGRAS10-RNAi plants were more tolerant to abiotic stresses (salt, drought, and Abscisic acid) than the WT plants. Down-regulation of SlGRAS10 significantly enhanced the expressions of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) to reduce the effects of reactive oxygen species (ROS) such as O2− and H2O2. Malondialdehyde (MDA) and proline contents were remarkably high in SlGRAS10-RNAi plants. Furthermore, the expression levels of chlorophyll biosynthesis, flavonoid biosynthesis, and stress-related genes were also enhanced under abiotic stress conditions. Collectively, our conclusions emphasized the significant function of SlGRAS10 as a stress tolerate transcription factor in a certain variety of abiotic stress tolerance by enhancing osmotic potential, flavonoid biosynthesis, and ROS scavenging system in the tomato plant.

6-Bromoindirubin-3′-Oxime Suppresses LPS-Induced Inflammation via Inhibition of the TLR4/NF-κB and TLR4/MAPK Signaling Pathways

Inflammation ◽  
2019 ◽  
Vol 42 (6) ◽  
pp. 2192-2204 ◽  
Author(s):  
Chang Liu ◽  
Xin Tang ◽  
Wenjing Zhang ◽  
Guohong Li ◽  
Yingyu Chen ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Mapk Signaling ◽  
Mapk Signaling Pathways

scholarly journals Computational Modeling of PI3K/AKT and MAPK Signaling Pathways in Melanoma Cancer

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0152104 ◽  
Author(s):  
Francesco Pappalardo ◽  
Giulia Russo ◽  
Saverio Candido ◽  
Marzio Pennisi ◽  
Salvatore Cavalieri ◽  
...  
Keyword(s):  
Computational Modeling ◽  
Signaling Pathways ◽  
Mapk Signaling ◽  
Melanoma Cancer ◽  
Mapk Signaling Pathways
Sign in / Sign up

Export Citation Format

Share Document