Recovery and Maintenance of NESTIN Expression in Umbilical Cord-MSC Using a Novel Culture Medium
Abstract Mesenchymal stem cells (MSC) are a popular candidate in cellular therapy for many diseases. It is well known by its feature of self-renewal and differentiation potential. NESTIN is a cytoskeletal protein that expresses in MSC and plays an important role in cell proliferation and differentiation. Here, we demonstrated that adding UltraGRO, which is a medium supplement, could maintain and partially recover the expression of NESTIN in human umbilical cord derived MSCs (UC-MSCs). Furthermore, the UC-MSCs cultured with UltraGRO showed a better immunomodulation ability in colitis mouse model compared with that cultured in other mediums. This indicates that the use of novel culture medium benefits to the maintenance of NESTIN expression and NESTIN may be one of the vital factors that regulate the performances of MSC.