scholarly journals Recovery and Maintenance of NESTIN Expression in Umbilical Cord-MSC Using a Novel Culture Medium

2020 ◽  
Author(s):  
Yuncheng Liu ◽  
Feidi Xiao ◽  
Xiang Hu ◽  
Zan Tang ◽  
Zeqin Fu ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Culture Medium ◽  
Cellular Therapy ◽  
Cytoskeletal Protein ◽  
Human Umbilical Cord ◽  
Nestin Expression ◽  
Differentiation Potential ◽  
Self Renewal ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

Abstract Mesenchymal stem cells (MSC) are a popular candidate in cellular therapy for many diseases. MSCs are well known by their feature of self-renewal and their differentiation potential. NESTIN is a cytoskeletal protein expressed in MSC that functions directly in cell proliferation and differentiation. Here, we demonstrated that adding UltraGRO, a medium supplement, could maintain and partially recover the expression of NESTIN in human umbilical cord derived MSCs (UC-MSCs). Furthermore, the UC-MSCs cultured with UltraGRO showed a better immunomodulation ability in a colitis mouse model compared with those cultured in other types of media. This indicates that the use of novel culture medium benefits the maintenance of NESTIN expression and NESTIN may be one of the vital factors that regulates the performance of MSCs.

scholarly journals Recovery and Maintenance of NESTIN Expression in Umbilical Cord-MSC Using a Novel Culture Medium

2020 ◽  
Author(s):  
Yuncheng Liu ◽  
Feidi Xiao ◽  
Xiang Hu ◽  
Zan Tang ◽  
Zeqin Fu ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Culture Medium ◽  
Cellular Therapy ◽  
Cytoskeletal Protein ◽  
Human Umbilical Cord ◽  
Nestin Expression ◽  
Differentiation Potential ◽  
Self Renewal ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

Abstract Mesenchymal stem cells (MSC) are a popular candidate in cellular therapy for many diseases. It is well known by its feature of self-renewal and differentiation potential. NESTIN is a cytoskeletal protein that expresses in MSC and plays an important role in cell proliferation and differentiation. Here, we demonstrated that adding UltraGRO, which is a medium supplement, could maintain and partially recover the expression of NESTIN in human umbilical cord derived MSCs (UC-MSCs). Furthermore, the UC-MSCs cultured with UltraGRO showed a better immunomodulation ability in colitis mouse model compared with that cultured in other mediums. This indicates that the use of novel culture medium benefits to the maintenance of NESTIN expression and NESTIN may be one of the vital factors that regulate the performances of MSC.

scholarly journals Regulation of GDNF expression in Sertoli cells

Reproduction ◽  
2019 ◽  
Author(s):  
Parag Parekh ◽  
Thomas Xavier Garcia ◽  
Marie-claude Hofmann
Keyword(s):  
Germ Cell ◽  
Sertoli Cells ◽  
Seminiferous Tubules ◽  
Receptor Complex ◽  
Myoid Cells ◽  
Self Renewal ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Direct Cell ◽  
Ability To Drive

Sertoli cells regulate male germ cell proliferation and differentiation and are a critical component of the spermatogonial stem cell (SSC) niche, where homeostasis is maintained by the interplay of several signaling pathways and growth factors. These factors are secreted by Sertoli cells located within the seminiferous epithelium, and by interstitial cells residing between the seminiferous tubules. Sertoli cells and peritubular myoid cells produce glial cell line-derived neurotrophic factor (GDNF), which binds to the RET/GFRA1 receptor complex at the surface of undifferentiated spermatogonia. GDNF is known for its ability to drive SSC self-renewal and proliferation of their direct cell progeny. Even though the effects of GDNF are well studied, our understanding of the regulation its expression is still limited. The purpose of this review is to discuss how GDNF expression in Sertoli cells is modulated within the niche, and how these mechanisms impact germ cell homeostasis.

High yield of human umbilical cord-derived mesenchymal stromal cells in an animal component-free culture medium

Cytotherapy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. S111-S112
Author(s):  
R. Wagey ◽  
K. Bertram ◽  
M. Elliott ◽  
A. Eaves ◽  
S. Szilvassy ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Culture Medium ◽  
High Yield ◽  
Human Umbilical Cord ◽  
Animal Component

scholarly journals Human umbilical-cord mesenchymal stem cells inhibit bacterial growth and alleviate antibiotic resistance in neonatal imipenem-resistant Pseudomonas aeruginosa infection

2019 ◽  
Vol 26 (3) ◽  
pp. 215-221 ◽  
Author(s):  
Zhuxiao Ren ◽  
Xuaner Zheng ◽  
Haoming Yang ◽  
Qi Zhang ◽  
Xiaohong Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pseudomonas Aeruginosa ◽  
Mesenchymal Stem Cells ◽  
Antibiotic Resistance ◽  
Umbilical Cord ◽  
Bacterial Growth ◽  
Culture Medium ◽  
Antimicrobial Properties ◽  
Neonatal Infection ◽  
Human Umbilical Cord

Human umbilical-cord mesenchymal stem cells (hUCMSCs) are a safe and convenient source of MSCs and have shown beneficial effects in neonatal infection and sepsis animal models. However, the factors leading to improved outcomes are still unclear. The aim of this study was to investigate the antibacterial effect and regulation of antimicrobial resistance of hUCMSCs. We separated imipenem-resistant Pseudomonas aeruginosa (PA) from neonates and incubated it with hUCMSCs as well as their culture medium. Assessment of direct inhibition of bacterial growth was done by counting CFUs. The concentration of antibacterial peptides in the culture medium of hUCMSCs was measured. Standard PA was inoculated with a sub-inhibitory concentration of imipenem with and without hUCMSC conditioned medium and antimicrobial peptides. The sensitivity to imipenem was detected until PA showed resistance to imipenem. Outer membrane protein (OprD2) mRNA expression in PA before and after the induction of imipenem resistance was analysed. We found that HUCMSCs possessed direct antimicrobial properties against bacteria and could alleviate antibiotic resistance via reserving OprD2 expression in PA.

Umbilical cord mesenchymal stem cells suppress B-cell proliferation and differentiation

2012 ◽  
Vol 274 (1-2) ◽  
pp. 46-53 ◽  
Author(s):  
Nan Che ◽  
Xia Li ◽  
Shiliang Zhou ◽  
Rui Liu ◽  
Dongyan Shi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
B Cell ◽  
Umbilical Cord ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

scholarly journals Human Umbilical Cord-Derived Mesenchymal Stem Cells Improve Learning and Memory Function in Hypoxic-Ischemic Brain-Damaged Rats via an IL-8-Mediated Secretion Mechanism Rather than Differentiation Pattern Induction

2015 ◽  
Vol 35 (6) ◽  
pp. 2383-2401 ◽  
Author(s):  
Xiaoqin Zhou ◽  
Jialu Gu ◽  
Yan Gu ◽  
Mulan He ◽  
Yang Bi ◽  
...  
Keyword(s):  
Learning And Memory ◽  
Umbilical Cord ◽  
Memory Function ◽  
Morphological Characteristics ◽  
Human Umbilical Cord ◽  
Therapeutic Effects ◽  
Differentiation Potential ◽  
Ischemic Brain ◽  
Differentiation Pattern

Background: MSCs are a promising therapeutic resource. Paracrine effects and the induction of differentiation patterns are thought to represent the two primary mechanisms underlying the therapeutic effects of mesenchymal stem cell (MSC) transplantation in vivo. However, it is unclear which mechanism is involved in the therapeutic effects of human umbilical cord-derived MSC (hUC-MSC) transplantation. Methods and Results: Based on flow cytometry analysis, hUC-MSCs exhibited the morphological characteristics and surface markers of MSCs. Following directed neural induction, these cells displayed a neuron-like morphology and expressed high levels of neural markers. All types of hUC-MSCs, including differentiated and redifferentiated cells, promoted learning and memory function recovery in hypoxic-ischemic brain damaged (HIBD) rats. The hUC-MSCs secreted IL-8, which enhanced angiogenesis in the hippocampus via the JNK pathway. However, the differentiated and redifferentiated cells did not exert significantly greater therapeutic effects than the undifferentiated hUC-MSCs. Conclusion: hUC-MSCs display the biological properties and neural differentiation potential of MSCs and provide therapeutic advantages by secreting IL-8, which participates in angiogenesis in the rat HIBD model. These data suggest that hUC-MSC transplantation improves the recovery of neuronal function via an IL-8-mediated secretion mechanism, whereas differentiation pattern induction was limited.

scholarly journals Therapeutic Effects of CUR-Activated Human Umbilical Cord Mesenchymal Stem Cells on 1-Methyl-4-phenylpyridine-Induced Parkinson’s Disease Cell Model

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Li Jinfeng ◽  
Wang Yunliang ◽  
Liu Xinshan ◽  
Wang Yutong ◽  
Wang Shanshan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Parkinson’S Disease ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Pc12 Cells ◽  
Umbilical Cord ◽  
Cell Model ◽  
Human Umbilical Cord ◽  
Therapeutic Effects ◽  
Proliferation And Differentiation

The purpose of this study is to evaluate the therapeutic effects of human umbilical cord-derived mesenchymal stem cells (hUC-MSC) activated by curcumin (CUR) on PC12 cells induced by 1-methyl-4-phenylpyridinium ion (MPP+), a cell model of Parkinson’s disease (PD). The supernatant of hUC-MSC and hUC-MSC activated by 5 µmol/L CUR (hUC-MSC-CUR) were collected in accordance with the same concentration. The cell proliferation and differentiation potential to dopaminergic neuronal cells and antioxidation were observed in PC12 cells after being treated with the above two supernatants and 5 µmol/L CUR. The results showed that the hUC-MSC-CUR could more obviously promote the proliferation and the expression of tyrosine hydroxylase (TH) and microtubule associated protein-2 (MAP2) and significantly decreased the expression of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) in PC12 cells. Furtherly, cytokines detection gave a clue that the expression of IL-6, IL-10, and NGF was significantly higher in the group treated with the hUC-MSC-CUR compared to those of other two groups. Therefore, the hUC-MSC-CUR may be a potential strategy to promote the proliferation and differentiation of PD cell model, therefore providing new insights into a novel therapeutic approach in PD.

scholarly journals In VitroDifferentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Peter Szaraz ◽  
Matthew Librach ◽  
Leila Maghen ◽  
Farwah Iqbal ◽  
Tanya A. Barretto ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Cardiac Regeneration ◽  
First Trimester ◽  
Human Umbilical Cord ◽  
Perivascular Cells ◽  
Differentiation Potential ◽  
Mortality And Morbidity ◽  
Cardiomyogenic Differentiation

Myocardial infarction (MI) causes an extensive loss of heart muscle cells and leads to congestive heart disease (CAD), the leading cause of mortality and morbidity worldwide. Mesenchymal stromal cell- (MSC-) based cell therapy is a promising option to replace invasive interventions. However the optimal cell type providing significant cardiac regeneration after MI is yet to be found. The aim of our study was to investigate the cardiomyogenic differentiation potential of first trimester human umbilical cord perivascular cells (FTM HUCPVCs), a novel, young source of immunoprivileged mesenchymal stromal cells. Based on the expression of cardiomyocyte markers (cTnT, MYH6, SIRPA, and CX43) FTM and term HUCPVCs achieved significantly increased cardiomyogenic differentiation compared to bone marrow MSCs, while their immunogenicity remained significantly lower as indicated by HLA-A and HLA-G expression and susceptibility to T cell mediated cytotoxicity. When applying aggregate-based differentiation, FTM HUCPVCs showed increased aggregate formation potential and generated contracting cells within 1 week of coculture, making them the first MSC type with this ability. Our results indicate that young FTM HUCPVCs have superior cardiomyogenic potential coupled with beneficial immunogenic properties when compared to MSCs of older tissue sources, suggesting thatin vitropredifferentiation could be a potential strategy to increase their effectivenessin vivo.

Sign in / Sign up

Export Citation Format

Share Document