Effects of Short-term Application of Moutai Lees Biochar on Nutrients and Fungal Community Structure in Yellow Soil of Guizhou

2021 ◽  
Author(s):  
Meng Zhang ◽  
Yanling Liu ◽  
Quanquan Wei ◽  
Jiulan Gou
Keyword(s):  
Community Structure ◽  
Microbial Biomass ◽  
Relative Abundance ◽  
Fungal Community ◽  
Pathogenic Bacteria ◽  
Plant Pathogens ◽  
Community Diversity ◽  
Microbial Biomass N ◽  
Short Term ◽  
Fungal Community Structure

Abstract In order to realize the resource utilization of Guizhou sauce-flavor distiller's grains and the improvement of yellow soil fertility, a field experiment was carried out to study the effects of short-term application of vinasse biochar on soil nutrients and the diversity of fungal community structure by setting five biochar dosages of 0% (MB0), 0.5% (MB0.5), 1.0% (MB1.0), 2.0% (MB2.0), and 4.0% (MB4.0). The results showed that the application of lees biochar increased the pH, soil organic matter (SOM), total nitrogen (TN), ammonium N (AN), nitrate N (NN), available phosphorus and available potassium of the yellow soil to varying degrees, but decreased the microbial biomass carbon (MBC) and microbial biomass N (MBN) by 12.36%-26.49% and 34.10%-59.95% respectively. The application of lees biochar significantly reduced the number of fungal OTUs and community diversity. Compared with MB0 treatment, the application of lees biochar significantly changed the structure of the fungal community. The relative abundances of Mortierellomycota, Basidiomycota, Glomeromycota, and Chlorophyta were all increased in varying degrees, but the relative abundance of Ascomycota was significantly reduced by 23.86%-29.06%. At the same time, the application of lees biochar also increased the relative abundance of some functional bacteria, such as Mortierella and Chaetomium, and reduced the relative abundance of some pathogenic bacteria, such as Aspergillus and Fusarium. In addition, the results of redundant analysis showed that SOM, AN and NN were the main environmental factors that affect the change of yellow soil fungal community structure. In summary, the short-term application of lees biochar can increase the nutrient content of soil, change the structure and diversity of soil fungal communities, and also can reduce the relative abundance of some pathogenic bacteria, which can inhibit the growth and reproduction of harmful plant pathogens.

scholarly journals Cultivated and wild pearl millet display contrasting patterns of abundance and co-occurrence in their root mycobiome

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Marie-Thérèse Mofini ◽  
Abdala G. Diedhiou ◽  
Marie Simonin ◽  
Donald Tchouomo Dondjou ◽  
Sarah Pignoly ◽  
...  
Keyword(s):  
Community Structure ◽  
Pearl Millet ◽  
Relative Abundance ◽  
Fungal Community ◽  
Management Practices ◽  
Pennisetum Glaucum ◽  
Fungal Communities ◽  
Fungal Community Structure ◽  
The Core ◽  
Factors Influencing

AbstractFungal communities associated with roots play a key role in nutrient uptake and in mitigating the abiotic and biotic stress of their host. In this study, we characterized the roots mycobiome of wild and cultivated pearl millet [Pennisetum glaucum (L.) R. Br., synonym: Cenchrus americanus (L.) Morrone] in three agro-ecological areas of Senegal following a rainfall gradient. We hypothesized that wild pearl millet could serve as a reservoir of endophytes for cultivated pearl millet. We therefore analyzed the soil factors influencing fungal community structure and whether cultivated and wild millet shared the same fungal communities. The fungal communities associated with pearl millet were significantly structured according to sites and plant type (wild vs cultivated). Besides, soil pH and phosphorus were the main factors influencing the fungal community structure. We observed a higher fungal diversity in cultivated compared to wild pearl millet. Interestingly, we detected higher relative abundance of putative pathotrophs, especially plant pathogen, in cultivated than in wild millet in semi-arid and semi-humid zones, and higher relative abundance of saprotrophs in wild millet in arid and semi-humid zones. A network analysis based on taxa co-occurrence patterns in the core mycobiome revealed that cultivated millet and wild relatives had dissimilar groups of hub taxa. The identification of the core mycobiome and hub taxa of cultivated and wild pearl millet could be an important step in developing microbiome engineering approaches for more sustainable management practices in pearl millet agroecosystems.

scholarly journals Adaptation of Soil Fungal Community Structure and Assembly to Long- Versus Short-Term Nitrogen Addition in a Tropical Forest

2021 ◽  
Vol 12 ◽  
Author(s):  
Jinhong He ◽  
Shuo Jiao ◽  
Xiangping Tan ◽  
Hui Wei ◽  
Xiaomin Ma ◽  
...  
Keyword(s):  
Tropical Forest ◽  
Community Composition ◽  
Relative Abundance ◽  
Fungal Community ◽  
N Addition ◽  
Short Term ◽  
Fungal Community Structure ◽  
Α Diversity ◽  
Soil Fungal Community

Soil fungi play critical roles in ecosystem processes and are sensitive to global changes. Elevated atmospheric nitrogen (N) deposition has been well documented to impact on fungal diversity and community composition, but how the fungal community assembly responds to the duration effects of experimental N addition remains poorly understood. Here, we aimed to investigate the soil fungal community variations and assembly processes under short- (2 years) versus long-term (13 years) exogenous N addition (∼100 kg N ha–1 yr–1) in a N-rich tropical forest of China. We observed that short-term N addition significantly increased fungal taxonomic and phylogenetic α-diversity and shifted fungal community composition with significant increases in the relative abundance of Ascomycota and decreases in that of Basidiomycota. Short-term N addition also significantly increased the relative abundance of saprotrophic fungi and decreased that of ectomycorrhizal fungi. However, unremarkable effects on these indices were found under long-term N addition. The variations of fungal α-diversity, community composition, and the relative abundance of major phyla, genera, and functional guilds were mainly correlated with soil pH and NO3––N concentration, and these correlations were much stronger under short-term than long-term N addition. The results of null, neutral community models and the normalized stochasticity ratio (NST) index consistently revealed that stochastic processes played predominant roles in the assembly of soil fungal community in the tropical forest, and the relative contribution of stochastic processes was significantly increased by short-term N addition. These findings highlighted that the responses of fungal community to N addition were duration-dependent, i.e., fungal community structure and assembly would be sensitive to short-term N addition but become adaptive to long-term N enrichment.

scholarly journals Assessment of Cu applications in two contrasting soils—effects on soil microbial activity and the fungal community structure

Ecotoxicology ◽  
2018 ◽  
Vol 27 (2) ◽  
pp. 217-233 ◽  
Author(s):  
Katharina M. Keiblinger ◽  
Martin Schneider ◽  
Markus Gorfer ◽  
Melanie Paumann ◽  
Evi Deltedesco ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Biomass ◽  
Fungal Community ◽  
Agricultural Soils ◽  
Sandy Loam ◽  
Oxidative Enzymes ◽  
Microbial Biomass C ◽  
Soil Microbial ◽  
Fungal Community Structure ◽  
Fungal Abundance

AbstractCopper (Cu)-based fungicides have been used in viticulture to prevent downy mildew since the end of the 19th century, and are still used today to reduce fungal diseases. Consequently, Cu has built up in many vineyard soils, and it is still unclear how this affects soil functioning. The present study aimed to assess the short and medium-term effects of Cu contamination on the soil fungal community. Two contrasting agricultural soils, an acidic sandy loam and an alkaline silt loam, were used for an eco-toxicological greenhouse pot experiment. The soils were spiked with a Cu-based fungicide in seven concentrations (0–5000 mg Cu kg−1 soil) and alfalfa was grown in the pots for 3 months. Sampling was conducted at the beginning and at the end of the study period to test Cu toxicity effects on total microbial biomass, basal respiration and enzyme activities. Fungal abundance was analysed by ergosterol at both samplings, and for the second sampling, fungal community structure was evaluated via ITS amplicon sequences. Soil microbial biomass C as well as microbial respiration rate decreased with increasing Cu concentrations, with EC50 ranging from 76 to 187 mg EDTA-extractable Cu kg−1 soil. Oxidative enzymes showed a trend of increasing activity at the first sampling, but a decline in peroxidase activity was observed for the second sampling. We found remarkable Cu-induced changes in fungal community abundance (EC50 ranging from 9.2 to 94 mg EDTA-extractable Cu kg−1 soil) and composition, but not in diversity. A large number of diverse fungi were able to thrive under elevated Cu concentrations, though within the order of Hypocreales several species declined. A remarkable Cu-induced change in the community composition was found, which depended on the soil properties and, hence, on Cu availability.

Response of microbial processes and fungal community structure to vegetation management in mixedwood forest soils

1998 ◽  
Vol 76 (12) ◽  
pp. 2002-2010 ◽  
Author(s):  
APC Houston ◽  
S Visser ◽  
R A Lautenschlager
Keyword(s):  
Community Structure ◽  
Microbial Biomass ◽  
Fungal Community ◽  
Mineral Soil ◽  
Vegetation Management ◽  
Basal Respiration ◽  
Microbial Processes ◽  
Microbial Biomass C ◽  
Fungal Community Structure ◽  
Decomposer Fungi

Previous research to determine the impacts of harvesting and site preparation on microbial processes and decomposer fungi in mixedwood forests indicated that effects were minimal. As an extension to this research, the present study was conducted to evaluate if microbial processes and decomposer fungi were sensitive to manual or chemical vegetation management in addition to harvesting and site preparation. The impact of vegetation management on microbial processes and decomposer fungi was examined in organic and mineral soil from harvested sites in northwestern Ontario. Three vegetation management treatments were applied to three clear-cut and prepared sites 2 years prior to the initiation of the study. Four treatments were established within each site: (i) harvested (control), (ii) glyphosate herbicide (Vision®), (iii) triclopyr herbicide (Release®), and (iv) manually operated brushsaws. The objective of the study was to determine the response of basal respiration, microbial biomass C, metabolic quotients (qCO2), microbial carbon : soil organic carbon ratio (Cmic/Corg), nitrogen mineralization, and fungal community structure to vegetation management by chemical and manual means. Relative to the harvested control, vegetation management had no significant impact on fungal community structure as evidenced through rank abundance curves and indices of fungal community richness, diversity, evenness, or dominance. Although the abundances of the majority of fungal species were not impacted by vegetation management, herbicide and brushsaw use decreased the isolation frequencies of Mortierella vinacea (Dixon-Stewart) and Paecilomyces carneus (Duché and Heim) Brown and Smith, respectively. Occurrence of Paecilomyces carneus was significantly greater in the organic soil than in the mineral soil of the harvested control, but this difference was not detectable in the blocks that had undergone vegetation management. Two years after vegetation management was imposed there were no detectable effects on basal respiration, microbial biomass C, qCO2, Cmic/Corg, or nitrogen mineralization in either the organic or mineral soil layers as compared with measurements made in the harvested control plots.Key words: harvesting, herbicide, brushsaw, fungal community, microbial processes.

scholarly journals Temporal Variability in the Rhizosphere Bacterial and Fungal Community Structure in the Melon Crop Grown in a Closed Hydroponic System

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 719
Author(s):  
Yu-Pin Lin ◽  
Chiao-Ming Lin ◽  
Hussnain Mukhtar ◽  
Hsiao-Feng Lo ◽  
Min-Chun Ko ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Relative Abundance ◽  
Fungal Community ◽  
Temporal Variability ◽  
Bacterial Community Structure ◽  
Fruit Weight ◽  
Growth Stages ◽  
Sequencing Analysis ◽  
Fungal Community Structure

Microbes can establish a pathogenetic or symbiotic relationship with plants in soil and aquatic ecosystems. Although change in bacterial and fungal community in soil and their interaction with plants have been widely studied, little is known about their community structure in hydroponic systems across plant growth stages under different nutrient treatments. This study used next-generation sequencing analysis to assess the temporal changes in melon rhizosphere bacterial and fungal community structure across six different nutrient treatments. We found significant changes in the microbial community composition (especially for bacteria) between growth stages (R = 0.25–0.63, p < 0.01) than nutrient treatments. Proteobacteria dominated the bacterial community at the phylum level across melon growth stages (59.8% ± 16.1%). The genera Chryseobacterium, Pseudomonas, and Massilia dominated the rhizosphere in the flowering and pollination stage, while Brevibacillius showed the highest relative abundance in the harvesting stage. However, the rhizosphere was dominated by uncultured fungal taxa, likely due to the application of fungicides (Ridomil MZ). Further, linear regression analysis revealed a weak influence of bacterial community structure on melon yield and quality, while fruit weight and quality moderately responded to Mg and K deficiency. Nevertheless, the relative abundance of bacterial genus Chryseobacterium in the vegetative stage showed a strong correlation with fruit weight (R2 = 0.75, p < 0.05), while genera Brevibacillus, Lysobacter, and Bosea in late growth stages strongly correlated with fruit sweetness. Overall, temporal variability in the microbial (especially bacterial) community structure exceeds the variability between nutrient treatments for the given range of nutrient gradient while having little influence on melon yield.

Microbial processes and fungal community structure in soils from clear-cut and unharvested areas of two mixedwood forests

1998 ◽  
Vol 76 (4) ◽  
pp. 630-640 ◽  
Author(s):  
APC Houston ◽  
S Visser ◽  
R A Lautenschlager
Keyword(s):  
Community Structure ◽  
Microbial Biomass ◽  
Nitrogen Mineralization ◽  
Fungal Community ◽  
Mineral Soil ◽  
Basal Respiration ◽  
Microbial Processes ◽  
Fungal Community Structure ◽  
Mixedwood Forests ◽  
The Impact

Decomposer fungi and microbial processes were examined in harvested and adjacent unharvested areas of two mixedwood forests in northwestern Ontario. Seven to 9 years following harvesting, organic and mineral soil from treated and control areas was sampled with the objective of examining the impact of clear-cutting and site preparation on soil basal respiration, microbial biomass carbon (C), metabolic quotients (qCO2), microbial C to organic C ratios (Cmic:Corg), nitrogen mineralization, and fungal community structure. Relative sensitivity of fungal community indices at species and genus levels was also examined. Soil microbial processes and fungal community structure were similar in harvested and unharvested stands. Although reductions were not significant, basal respiration, microbial biomass C, qCO2, Cmic:Corg, and nitrogen mineralization tended to be lower in organic soil from the harvested sites than the unharvested sites, with the exception of qCO2. Fungal community structure indicated by rank abundance curves and indices of fungal richness, diversity, evenness, and dominance was similar in harvested and unharvested soil. When comparing organic and mineral soil layers, fungal richness, diversity, and community composition corresponded more closely in the harvested sites than unharvested sites. The isolation frequencies of all frequently found fungal species were equivalent in harvested and unharvested soil. In this study, identifying fungi to the genus level instead of the species level did not alter any major conclusions.Key words: harvesting, fungal community, microbial processes.

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