Fine Mapping and Candidate Gene Analysis of the Up Locus Determining Fruit Orientation in Pepper (Capsicum spp.)
Abstract Fruit orientation is an important horticultural and domesticated trait, which is controlled by a single semi-dominant gene (up) in pepper. However, the gene underlying up locus has not yet been identified. In this study, the previously detected major QTL UP12.1 was firstly verified using an intraspecific backcross population (n=225) stem from the cross of BB3 (C. annuum) and its wild relative Chiltepin (C. annuum var. glabriusculum) using BB3 as the recurrent parent. Then, a large BC1F2 population (n=1827) was used for recombinant screening to delimit the up locus into an interval with ~169.51 kb in length. Sequence comparison and expression analysis suggested that Capana12g000958, encoding a developmentally regulated G-protein 2, was the most likely candidate gene for up. The findings of this study will form a basis for gene isolation and reveal of genetic mechanism underlying the fruit orientation domestication in pepper.