Organic acids induce by metal-tolerant Pantoea sp. WP-5 and biogas residues enhanced phytostabilization of cadmium in soil

Abstract This study investigated the phytoremediation potential of maize (Zea mays L.) in Cd contaminated soil through co-inoculation of metal tolerant plant beneficial rhzobacteria (MtPBR: Pantoea sp. strain WP-5) with organic manures (PM: poultry manure and BGR: biogas residues). The objectives of this study were to i) examine comparative efficiency of MtPBR, PM and BGR alone or in combined form to improve maize biomass and physiology, and ii) understand the role of organic acid production in root exudates of maize for Cd accumulation and translocation. Pantoea sp. WP-5 showed tolerance to high Cd concentration (1000 mg L− 1), thereby inoculated to maize seeds sown in soil spiked with 75 mg Cd kg− 1 soil and 500 g each of the organic manures per pot. The co-inoculation of MtPBR + BGR significantly (P < 0.05) increased chlorophyll contents, root/shoot dry weight, photosynthetic rate, stomatal conductance and relative water contents, whereas decreased electrolyte leakage, malondialdehyde contents, ascorbate peroxidase and catalase activity in maize over the control treatment. The co-inoculation of MtPBR + BGR produced significantly (P < 0.05) higher concentrations of acetic and citric acid (52.7 ± 0.5 and 22.8 ± 0.08 µg g− 1 root fwt, respectively) in root exudates of maize, which immobilized Cd within plant roots inferred by the positive relation (root Cd vs. organic acids; R2 = 0.80–0.92) and reduced Cd translocation to shoots inferred by the negative relation (shoot Cd vs. organic acids; R2 = 0.81–0.90). It is concluded that the application of MtPBR + BGR enhanced organic acid induced phyto-stabilization and accumulation of Cd in roots and restricted its translocation to shoots.

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INKUBASI TITONIA PADA TANAH SAWAH TERHADAP ASAM-ASAM ORGANIK

Jurnal Solum ◽

10.25077/js.7.1.7-18.2010 ◽

2010 ◽

Vol 7 (1) ◽

pp. 7 ◽

Cited By ~ 2

Author(s):

Gusnidar Gusnidar ◽

Nurhajati Hakim ◽

Teguh Budi Prasetyo

Keyword(s):

Organic Acids ◽

Organic Acid ◽

Acid Production ◽

Rice Field ◽

Soil Surface ◽

Field Capacity ◽

Dry Weight ◽

Organic Acid Production

The research about “Incubation of titonia for organic acid production in rice field” has been conducted in soil laboratory Agriculture Collage Andalas University Padang, since January to August 2005. The objective of this research was to determine the best method of using titonia to produce organic acids in rice field. The research had 2 factors, 3 series of titonia application and 5 levels of titonia dossages with 3 replications. The series are: A1 = Titonia was mixed with soil and then, the soil was directly flooded; A2 = Titonia was mixed with soil and then, let them stay on soil surface for 3 weeks before being flooded; A3 = Titonia was mixed with soil and then, the soil was moistened until field capacity for 3 weeks before being flooded. The second factor was titonia dossages (0,000; 1,250; 2,500; 3,750; 5,000 g dry weight of titonia per kg soil). The result showed that organic acids of the soil had been changed as a consequence of titonia application and the incubation with soil. The highest organic acid production was found at combination of titonia incubation with soil at field capacity for 3 weeks, that was malat acid (184-489 mgl-1). The others detected were citrate, tartarate, malate, and benzoat acids, butt fenolate acids was not detected. Keywords: incubation, organic acids, rice field, titonia.

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DETECT ACTIVITY OF SOME BIOLOGICAL FACTORS TO INDUCE RESISTANCE IN CANTALOUPE PLANT THROUGH PEROXIDASE ENZYME , PHENOLS AND CHLOROPHYLL CONTENTS

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v48i5.333 ◽

2017 ◽

Vol 48 (5) ◽

Author(s):

Hashem & et al.

Keyword(s):

Chlorophyll Content ◽

Growth Parameters ◽

Pathogenic Fungus ◽

Dry Weight ◽

Macrophomina Phaseolina ◽

Control Treatment ◽

Chlorophyll Contents ◽

Peroxidase Enzyme ◽

Induce Resistance ◽

Treated Plant

The aim of this study was to evaluate efficiency of some biological agents to reduce charcoal rot of Cantaloupe which is caused by Macrophomina phaseolina under wooden canopy and to detect activity of inducer agents through Peroxidase enzyme, phenols and chlorophyll contents. Results obtained under Wooden canopy, treatment of combination between T.viride and B.subtilis was superior on other treatments. The disease percentage and disease severity were (0.0), (0.0)% respectively compared to control treatment (pathogen) 88.3 and 76.6% respectively. However treatment of integration between T.viride and B.subtilis was superior on other treatments in antagonism against M.phaseolina and to improve growth parameters of cantaloupe and increased wet weight and dry weight of vegetation and root to (35.0 – 4.0), (13.7 – 2.4) g/ plant respectively. Results indicated that treated plant produced phenols and peroxidase enzyme. Treatments of T.viride with B.subtilis or T.viride with A.chrococcum showed superiority in inducing peroxidase enzyme. The change in light absorbance/ min/ g .Fresh weight of cantaloupe were 81.2 and 80.6 respectively, compared to control treatment (pathogenic fungus) the activity of Peroxidase was 36.9. the same trend reflected to increase activity of phenols in treatments of T.viride with B.subtilis or T.viride with A.chrococcum reached (5.21 and 5.00) mg/g. compared with control treatment 3.6 mg/g. The result, also showed that all treatments increased chlorophyll content. Integration between T.viride and B.subtilis achieved highest chlorophyll content (56.3) Spad.

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Bioleaching Ability of Fungi Isolated from an Indonesian Sulfurous River Sediment

Indonesian Journal of Chemistry ◽

10.22146/ijc.44810 ◽

2020 ◽

Vol 20 (4) ◽

pp. 810

Author(s):

Serafica Btari Christiyani Kusumaningrum ◽

I Wayan Warmada ◽

Wahyu Wilopo ◽

Endah Retnaningrum

Keyword(s):

Organic Acids ◽

Organic Acid ◽

High Performance ◽

River Sediment ◽

Low Cost ◽

Absorption Spectrometry ◽

Manganese Concentration ◽

Environmental Damage ◽

Organic Acid Production ◽

Phenotypic Characters

The unique characteristics of sulfurous river sediment located in Ungaran, Indonesia, are a reservoir of novel fungi with manganese bioleaching properties. Fungi are known to produce metabolic organic acids that have a potential for the industrial application of leaching metal from the ores. This application has high advantages, including low cost, low energy, and creates minimal environmental damage. Therefore, this research was performed to analyze the manganese bioleaching activities of two fungal isolates (KA2B2 and KB4B) from Indonesian sulfurous river sediment on pyrolusite and determine their phenotypic characters. These activities were investigated in terms of changes in fungal biomass, soluble manganese concentration, pH reduction, and organic acid production during 16 days of leaching. Soluble manganese concentrations were measured by atomic absorption spectrometry (AAS), whereas organic acid concentrations were analyzed by high-performance liquid chromatography (HPLC). According to bioleaching investigations, KA2B2 strain was more efficient than KB4B1 strain in extracting manganese from 0.02 g/cm3 pyrolusite. It also produced higher levels of organic acids, such as oxalic acid and citric acid, than KB4B1 strain, proving that strain of KA2B2 could be used to extract manganese from pyrolusite. Based on the phenotypic characters, both strains were identified as genus Penicillium.

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Disruption of a putative mitochondrial oxaloacetate shuttle protein in Aspergillus carbonarius results in secretion of malic acid at the expense of citric acid production

BMC Biotechnology ◽

10.1186/s12896-019-0572-0 ◽

2019 ◽

Vol 19 (1) ◽

Author(s):

Lei Yang ◽

Tore Linde ◽

Abeer H. Hossain ◽

Mette Lübeck ◽

Peter J. Punt ◽

...

Keyword(s):

Citric Acid ◽

Organic Acids ◽

Organic Acid ◽

Malic Acid ◽

Acid Production ◽

Mitochondrial Membrane ◽

Citric Acid Production ◽

Aspergillus Carbonarius ◽

Transcription Analysis ◽

Organic Acid Production

Abstract Background In filamentous fungi, transport of organic acids across the mitochondrial membrane is facilitated by active transport via shuttle proteins. These transporters may transfer different organic acids across the membrane while taking others the opposite direction. In Aspergillus niger, accumulation of malate in the cytosol can trigger production of citric acid via the exchange of malate and citrate across the mitochondrial membrane. Several mitochondrial organic acid transporters were recently studied in A. niger showing their effects on organic acid production. Results In this work, we studied another citric acid producing fungus, Aspergillus carbonarius, and identified by genome-mining a putative mitochondrial transporter MtpA, which was not previously studied, that might be involved in production of citric acid. This gene named mtpA encoding a putative oxaloacetate transport protein was expressed constitutively in A. carbonarius based on transcription analysis. To study its role in organic acid production, we disrupted the gene and analyzed its effects on production of citric acid and other organic acids, such as malic acid. In total, 6 transformants with gene mtpA disrupted were obtained and they showed secretion of malic acid at the expense of citric acid production. Conclusion A putative oxaloacetate transporter gene which is potentially involved in organic acid production by A. carbonarius was identified and further investigated on its effects on production of citric acid and malic acid. The mtpA knockout strains obtained produced less citric acid and more malic acid than the wild type, in agreement with our original hypothesis. More extensive studies should be conducted in order to further reveal the mechanism of organic acid transport as mediated by the MtpA transporter.

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Root organic acid exudates and properties of rhizosphere soils of white spruce (Picea glauca) and subalpine fir (Abies lasiocarpa)

Canadian Journal of Soil Science ◽

10.4141/cjss08021 ◽

2009 ◽

Vol 89 (3) ◽

pp. 287-300 ◽

Cited By ~ 26

Author(s):

M. M.S. Tuason ◽

J. M. Arocena

Keyword(s):

Organic Acids ◽

Organic Acid ◽

Root Exudates ◽

Picea Glauca ◽

Chemical Properties ◽

Exchange Capacity ◽

Soil Samples ◽

Abies Lasiocarpa ◽

Soil Extracts ◽

Exchangeable Ca

Rhizosphere processes, such as the production and release of organic acids, contribute to differences in properties between rhizosphere (R) and nonrhizosphere (NR) soils. We compared the organic acid composition/concentrations in the R and NR soil extracts of Picea glauca and Abies lasiocarpa, and in the root exudates of the same tree species. We also evaluated the chemical properties and mineral composition of the soil samples. Higher concentrations of organic acids were observed in R than in NR soils with aliphatic acids predominating in both soil extracts and root exudates. Acetic, formic, and protocatechuic acids were the predominant aliphatic and aromatic organic acids in R and NR soil extracts. Several organic acids in R soil extracts were not detected in NR soil extracts, with the reverse being true as well. Malonic and oxalic acids were predominant in the root exudates of both plant species. The total organic acid concentration was higher in P. glauca than in A. lasiocarpa. Glutaric and isocitric acids were found only in A. lasiocarpa, while gluconic, succinic, and protocatechuic acids were exclusive to P. glauca. Acetic and formic acids were detected in all R soil extracts, but not in root exudates. Glutaric acid (in P. glauca) and gluconic/succinic acids (A. lasiocarpa) were undetectable in root exudates, but were present in the corresponding R soil extracts. Soil pH was lower in the R than NR soil of P. glauca. Cation exchange capacity, and exchangeable Ca2+ and K+ were higher in R than in NR soils except for the A. lasiocarpa LFH horizon. Chlorite, mica, kaolinite, and vermiculite were detected in all soil samples. Smectite was detected more frequently in R than NR soils. Our results suggest that high amounts of organic acids might have played a significant role in the transformation of mica and chlorite into smectite in R soils.Key words: Rhizosphere, root exudates, organic acids

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The Effects of Foliar Spraying of Folic Acid and Cysteine on Growth, Chemical Composition of Leaves and Green Yield of Faba Bean (Vicia faba L.)

Basrah Journal of Agricultural Sciences ◽

10.37077/25200860.2019.212 ◽

2019 ◽

Vol 32 (2) ◽

pp. 223-229

Author(s):

Azhar W.A. Al-Maliky ◽

Awatif N. Jerry ◽

Faris I. Obead

Keyword(s):

Folic Acid ◽

Vicia Faba ◽

Faba Bean ◽

Growth Parameters ◽

Dry Weight ◽

Control Treatment ◽

Branch Number ◽

Shoot Height ◽

Chlorophyll Contents ◽

Combination Treatments

An experiment was conducted during winter growing season of 2018 /2019 at Al-Qurnah sub-district, Basrah Governorate to study the effect of foliar spraying of folic acid and amino acid cysteine at different concentrations on growth, Minerals, chlorophyll contents and green yield faba bean (Vicia faba cv. Luz de otono). The study included sixteen treatments Consist of four concentration of folic acid (o,10,20 and 30) mg.L-1 in combination with four concentrations of Cysteine (0,10,20 and 30) mg.L-1. The results indicated that spraying with folic acid or cysteine significantly increased all vegetative growth characteristics (shoot height, branch number, leaflet number and fresh and dry weight of shoot system per plant), N, P, K, chlorophyll content of leaves and green pod yield/plant and fresh seed yields /plant. Spraying with folic acid at 30 mg.L.-1 and cysteine at 30 mg.L.-1 gave the greatest values of growth parameters, chlorophyll and yield, whereas 20 mg.L-1 folic acid with 30 mg.L-1 Cysteine was produced the highest values of N, P and K in faba bean leaves as compared with other combination treatments. Control treatment gave the lowest values for growth parameters, chlorophyll, N, P, K and yield.

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Evaluación de la producción de ácidos orgánicos por Streptomyces spp. y solubilización de tres fuentes de fósforo por la cepa T3A

Revista Colombiana de Biotecnología ◽

10.15446/rev.colomb.biote.v17n1.41667 ◽

2015 ◽

Vol 17 (1) ◽

pp. 111-121 ◽

Cited By ~ 1

Author(s):

Gloria Carolina Prieto Correal ◽

Luis Daniel Prada Salcedo ◽

Claudia Liliana Cuervo Patiño ◽

Marcela Franco Correa

Keyword(s):

Organic Acids ◽

Organic Acid ◽

Acid Production ◽

Growth Promotion ◽

Morphological Characteristics ◽

Organic Acid Production ◽

Streptomyces Spp ◽

Colombian Andes ◽

Palabras Clave ◽

Ph Range

Título en ingles: Evaluation of organic acid production by Streptomyces spp. and solubilization of three phosphorus sources by strain T3A Título corto: Producción de ácidos orgánicos por Streptomyces spp. y solubilización de fosfatos por la cepa T3A. Título corto en ingles: Organic acid production by Streptomyces spp. and solubilization by strain T3AResumen: Quince aislamientos de actinobacterias solubilizadoras de fósforo obtenidas a partir de suelos de los andes orientales colombianos fueron identificadas por sus características morfológicas y por la secuenciación del gen 16S ADNr. El análisis BLASTN de las 15 secuencias obtenidas mostró que los aislamientos pertenecían al género Streptomyces. Paralelamente, los aislamientos fueron sometidos a la detección de ácidos orgánicos, durante el proceso de solubilización de fósforo con la presencia mayoritaria de los ácidos oxálico, cítrico y glucónico. Dentro de las cepas evaluadas Streptomyces sp. T3A fue seleccionada para ser evaluada bajo diferentes fuentes de fósforo inorgánico debido a los resultados de evaluaciones cualitativas y cuantitativas realizadas previamente, en las cuales mostró una actividad solubilizadora de fósforo significativamente alta. Los resultados evidenciaron la capacidad de ésta actinobacteria para solubilizar diferentes fuentes de fosfatos insolubles con valores de 122 mgP·L-1 paraCa3(PO4)2, 14 mgP·L-1 para AlPO4 y 19,6 mgP·L-1 para roca fosfórica. También los ensayos revelaron que la actividad se mantiene en un rango de pH de 5 a 8 con las mismas fuentes de fosfatos evaluadas. Los resultados presentados contribuyen al avance en la caracterización de estas bacterias como promotoras de crecimiento vegetal con el fin de presentarlos como un recurso clave a nivel de biotecnología agrícola. Palabras clave: Streptomyces, Solubilización de fósforo, ácidos orgánicos, actinobacteria, HPLC. Abstrac: Fifteen isolates of Eastern Cordillera of the Colombian Andes were identified by morphological characteristics and 16S rDNA gene sequence. The BLAST analysis of 15 sequences shows that isolates belong to Streptomyes. Also we detected the organic acids in the solubilization process mainly oxalic acid, citric acid and gluconic acid. Streptomyces sp. (T3A) was selected in preliminary qualitative and quantitative assays by the high phosphorus solubilizing activity; in this work we evaluate this strain with different forms of inorganic phosphate. The results evidenced the capacity of this actinobacteria to solubilize phosphorous showed 122 mgP•L-1 Ca3(PO4)2, 14 mgP•L-1 AlPO4 and 19,6 mgP•L-1 for rock phosphate. Also the assays revealed that the activity was maintained between a pH range of 5 to 8 with the same sources of insoluble phosphates evaluated. These results contribute to characterize these strains as plant growth promotion bacteria and as key source in agricultural biotech. Key words: Streptomyces, phosphate solubilization, organic acids, actinobacteria, HPLC. Recibido: octubre 10 de 2014 Aprobado: abril 28 de 2015

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Screening of a highly inhibitor-tolerant bacterial strain for 2,3-BDO and organic acid production from non-detoxified corncob acid hydrolysate

AMB Express ◽

10.1186/s13568-019-0879-1 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Jing Wu ◽

Yu-Jie Zhou ◽

Wen Zhang ◽

Ke-Ke Cheng ◽

Hong-Juan Liu ◽

...

Keyword(s):

Organic Acids ◽

Organic Acid ◽

Acid Production ◽

Enterobacter Cloacae ◽

Fermentation Inhibitors ◽

Organic Acid Production ◽

Inhibitor Tolerance ◽

Acid Hydrolysate ◽

Lignocellulose Hydrolysate ◽

Corncob Acid Hydrolysate

Abstract Fermentation of chemicals from lignocellulose hydrolysate is an effective way to alleviate environmental and energy problems. However, fermentation inhibitors in hydrolysate and weak inhibitor tolerance of microorganisms limit its development. In this study, atmospheric and room temperature plasma mutation technology was utilized to generate mutant strains of Enterobacter cloacae and screen for mutants with high inhibitor tolerance to acid hydrolysate of corncobs. A highly inhibitor-tolerant strain, Enterobacter cloacae M22, was obtained after fermentation with non-detoxified hydrolysate, and this strain produced 24.32 g/L 2,3-butanediol and 14.93 g/L organic acids. Compared with that of the wild-type strain, inhibitor tolerance was enhanced twofold with M22, resulting in improvement of 2,3-butanediol and organic acid production by 114% and 90%, respectively. This work presents an efficient method to screen for highly inhibitor-tolerant strains and evidence of a novel strain that can produce 2,3-butanediol and organic acids using non-detoxified acid hydrolysate of corncobs.

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Pseudomonas-aided zinc application improves the productivity and biofortification of bread wheat

Crop and Pasture Science ◽

10.1071/cp17441 ◽

2018 ◽

Vol 69 (7) ◽

pp. 659 ◽

Cited By ~ 26

Author(s):

Abdul Rehman ◽

Muhammad Farooq ◽

Muhammad Naveed ◽

Levent Ozturk ◽

Ahmad Nawaz

Keyword(s):

Grain Yield ◽

Organic Acid ◽

Bread Wheat ◽

Root Exudates ◽

Acid Production ◽

Foliar Application ◽

Seed Priming ◽

Organic Acid Production ◽

Soil Application ◽

Zn Concentration

Zinc (Zn) deficiency reduces the grain yield and quality of wheat. Plant-growth-promoting bacteria may help to improve plant Zn availability. This study evaluated the influence of inorganic Zn, with and without Zn-solubilising bacteria (Pseudomonas sp. MN12), on performance and grain biofortification of wheat. Zinc was supplied with and without Pseudomonas to two bread wheat (Triticum aestivum L.) cultivars (Lasani-2008 and Faisalabad-2008) via four treatments: soil application (5.0 mg kg–1 soil), foliar application (0.025 m), seed priming (0.5 m) and seed coating (1.25 g kg–1 seed). Hydroprimed seeds were taken as control. Inoculation with Pseudomonas improved photosynthesis, yield, biofortification of grains and organic acid production in root exudates. However, inoculation was more effective when applied in combination with different Zn application methods. Maximum improvement in plant photosynthetic assessment and grain yield was recorded with Pseudomonas + Zn seed priming, followed by Pseudomonas + soil application of Zn. These two combinations also enhanced organic acid production in root exudates of wheat. Soil and foliar application of Zn with Pseudomonas enhanced Zn concentration in whole grain, embryo, aleurone and endosperm. Combined application of Pseudomonas and Zn (soil and foliar) reduced phytate concentration and [phytate] : [Zn] ratio and increased the bioavailable Zn in wheat grain compared with the control. In conclusion, the combination Pseudomonas inoculation along with Zn seed priming and soil application was more effective in improving grain yield of wheat, whereas soil- and foliar-applied Zn with Pseudomonas inoculation was better in enhancing grain Zn concentration and bioavailability.

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Commercially available organic manures on growth, yield and nutrient content of Burmese Grapes (Baccaurea ramiflora)

Journal of Biodiversity Conservation and Bioresource Management ◽

10.3329/jbcbm.v7i1.57124 ◽

2022 ◽

Vol 7 (1) ◽

pp. 63-72

Author(s):

MR Munna ◽

SA Trisa ◽

MM Islam ◽

MJ Islam ◽

DC Pal ◽

...

Keyword(s):

Leaf Area Index ◽

Leaf Area ◽

Plant Height ◽

Chemical Properties ◽

Growth Yield ◽

Dry Weight ◽

Control Treatment ◽

Fresh Weight ◽

Area Index ◽

Organic Manures

A pot experiment was carried out in the net house of the Department of Soil, Water and Environment, University of Dhaka to evaluate the effectS of different organic manures on the growth performance, biomass production, NPKS content in leaf, stem and root of Burmese grapes (Baccaurea ramiflora) and some chemical properties of post- harvest soil. Two-month old Burmese grapes seedlings were planted in different organic manure-treated soils (viz. ACI, BGF, Bhola, Green Life, Kazi, Modern, Mazim, Payel, Payel Earth, and Shebok). A significant variation was observed among the treatments. Compared to control treatment, application of organic manures exhibited a significant influence on the physiological growth, viz. plant height (30.4 - 34.06 cm), leaf area (64.2 - 71.30 cm2/plant), leaf area index (0.17 - 0.19), fresh weight (32.2 - 53.06 g/plant), and dry weight (22.09 - 41.24 g/plant) after 120 days of the experiment. The highest plant height, leaf area, leaf area index, fresh weight, and dry weight were recorded in Kazi, BGF, Payel and Payel Earth, respectively. Similarly, the concentrations of N, P, K, and S in leaf, stem and root, and their uptakes were found significantly higher than the control. The highest N, P, K and S uptake by leaf, stem and root ranged from 37.66 - 41.69 kg/ha, 0.70 - 0.92 g/ha, 66.19 - 84.926 kg/ha and 6.82 - 10.11 kg/ha, respectively. Protein content was the highest in Payel in leaf (8%) and root (5%), and stem (7%) in Modern manure treatment. The overall results revealed that Kazi and Payel manures might be better for raising strong and healthy Burmese grape seedlings. J. Biodivers. Conserv. Bioresour. Manag. 2021, 7(1): 63-72

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