scholarly journals DETECT ACTIVITY OF SOME BIOLOGICAL FACTORS TO INDUCE RESISTANCE IN CANTALOUPE PLANT THROUGH PEROXIDASE ENZYME , PHENOLS AND CHLOROPHYLL CONTENTS

2017 ◽  
Vol 48 (5) ◽  
Author(s):  
Hashem & et al.

The aim of this study was to evaluate efficiency of some biological agents to reduce charcoal rot of Cantaloupe which is caused by Macrophomina  phaseolina  under wooden canopy and to detect activity of inducer  agents through Peroxidase enzyme, phenols and chlorophyll contents. Results obtained under Wooden canopy, treatment of combination between T.viride and B.subtilis was superior on other treatments. The disease percentage and disease severity were (0.0), (0.0)% respectively compared to control treatment (pathogen) 88.3 and 76.6% respectively. However treatment of integration between T.viride and B.subtilis was superior on other treatments in antagonism against M.phaseolina and to improve growth parameters of cantaloupe and increased wet weight and dry weight of vegetation and root to (35.0 – 4.0), (13.7 – 2.4) g/ plant respectively. Results indicated that treated plant produced phenols and peroxidase enzyme. Treatments of   T.viride with  B.subtilis or T.viride with  A.chrococcum showed superiority in inducing peroxidase enzyme. The change in light absorbance/ min/ g .Fresh weight of cantaloupe were 81.2 and 80.6 respectively, compared to control treatment (pathogenic fungus) the activity of Peroxidase was 36.9. the same trend reflected to increase activity of phenols in treatments of T.viride with  B.subtilis or T.viride with  A.chrococcum reached (5.21 and 5.00) mg/g. compared with control treatment 3.6 mg/g. The result, also showed that all treatments increased chlorophyll content. Integration between T.viride and  B.subtilis achieved highest chlorophyll content (56.3) Spad.

2019 ◽  
Vol 32 (2) ◽  
pp. 223-229
Author(s):  
Azhar W.A. Al-Maliky ◽  
Awatif N. Jerry ◽  
Faris I. Obead

An experiment was conducted during winter growing season of  2018 /2019 at Al-Qurnah sub-district, Basrah Governorate to study the effect of foliar spraying of folic acid and amino acid cysteine at different concentrations on growth, Minerals, chlorophyll contents and green yield faba bean (Vicia faba cv. Luz de otono). The study included sixteen treatments Consist of four concentration of folic acid (o,10,20 and 30) mg.L-1 in combination with four concentrations of Cysteine (0,10,20 and 30) mg.L-1. The results indicated that spraying with folic acid or cysteine significantly increased all vegetative growth characteristics (shoot height, branch number, leaflet number and fresh and dry weight of shoot system per plant), N, P, K, chlorophyll content of leaves and green pod yield/plant and fresh seed yields /plant. Spraying with folic acid at 30 mg.L.-1 and cysteine at 30 mg.L.-1 gave the greatest values of growth parameters, chlorophyll and yield, whereas 20 mg.L-1 folic acid with 30 mg.L-1 Cysteine was produced the highest values of N, P and K in faba bean leaves as compared with other combination treatments. Control treatment gave the lowest values for growth parameters, chlorophyll, N, P, K and yield.


1996 ◽  
Vol 121 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Ursula Schuch ◽  
Richard A. Redak ◽  
James Bethke

Six cultivars of poinsettia (Euphorbia pulcherrima Wind.), `Angelika White', `Celebrate 2', `Freedom Red', `Lilo Red', `Red Sails', and `Supjibi Red' were grown for 9 weeks during vegetative development under three constant-feed fertilizer treatments, 80,160, or 240 mg N/liter and two irrigation regimes, well-watered (high irrigation) or water deficient (low irrigation). Plants fertilized with 80 or 240 mg N/liter were 10% to 18% shorter, while those fertilized with 160 mg N/liter were 25 % shorter with low versus high irrigation. Leaf area and leaf dry weight increased linearly in response to increasing fertilizer concentrations. Low irrigation reduced leaf area, leaf, stem, and shoot dry weight 3670 to 41%. Cultivars responded similarly to irrigation and fertilizer treatments in all components of shoot biomass production and no interactions between the main effects and cultivars occurred. Stomatal conductance and transpiration decreased with increasing fertilizer rates or sometimes with low irrigation. Highest chlorophyll contents occurred in leaves of `Lilo Red' and `Freedom Red'. Leaves of plants fertilized with 80 mg N/liter were deficient in leaf N and had 40 % to 49 % lower leaf chlorophyll content compared to plants fertilized with 160 or 240 mg N/liter. Irrigation had no effect on leaf N or chlorophyll content. At the end of the experiment leaves of `Supjibi Red' and `Angelika White' contained higher concentrations of soluble proteins than the other four cultivars.


Author(s):  
Y. Rajasekhara Reddy ◽  
G. Ramanandam ◽  
P. Subbaramamma ◽  
A. V. D. Dorajeerao

A field experiment was carried out during rabi season of 2018-2019, at college farm, College of Horticulture, Dr. Y.S.R. Horticultural University, Venkataramannagudem, West Godavari District, Andhra Pradesh. The experiment was laidout in a Randomised Block Design with eleven treatments (viz., T1- NAA @ 50 ppm, T2-NAA @ 100 ppm, T3-GA3 @ 50 ppm,  T4-GA3 @ 100 ppm, T5-Thiourea @ 250 ppm, T6-Thiourea @ 500 ppm, T7-28-Homobrassinolide @ 0.1 ppm, T8-28-Homobrassinolide @ 0.2 ppm, T9-Triacontinol @ 2.5 ppm, T10-Triacontinol @ 5 ppm, T11-(Control) Water spray) and three replications. The treatments were imposed at 30 and 45 DAT in the form of foliar spray. Foliar application of GA3@ 100 ppm (T4) had recorded the maximum plant height (108.20 cm), leaf area (9.53 cm2) and leaf area index (0.74). Foliar application of thiourea @ 250 ppm (T5) had recorded the maximum values with respect to number of primary branches (15.03 plant-1), number of secondary branches (83.40 plant-1), plant spread (1793 cm2 plant-1), fresh weight (376.29 g plant-1), dry weight (103.54 g plant-1) and number of leaves plant-1((298.8). The same treatment (T5) had recorded the highest values with respect to crop growth rate (1.44 gm-2d-1), chlorophyll-a (1.40 mg g-1), chlorophyll-b (0.076 mg g-1) and total chlorophyll contents (1.48 mg g-1) in the leaves.


2020 ◽  
Vol 31 (2) ◽  
pp. 127-137
Author(s):  
Chatarina Lilis Suryani ◽  
◽  
Tutik Dwi Wahyuningsih ◽  
Supriyadi Supriyadi ◽  
Umar Santoso ◽  
...  

Plant leaves are the primary source of natural colorants for food, mainly due to their chlorophyll content. However, the plant types and the degree of leaf maturity determine the quality and quantity of the chlorophyll. This study aimed to determine the best maturity level of pandan (Pandanus amaryllifolius Roxb.) leaves that serves as potential source of chlorophyll for natural food colorants. Eighty three pandan plants obtained from six different farming locations in Bantul Regency, Yogyakarta, Indonesia were used as samples. The leaves were grouped into four levels of maturity using descriptive statistics based on their morphology, anatomy, color, and chlorophyll contents. The results showed that the average number of leaves ranged from 20-24 leaves per plant (at 95% confidence interval), and 96.4% of the plant had a maximum of 24 leaves. The leaf maturity was grouped into (1) young, (2) medium, (3) mature, and (4) over mature, corresponding to leaf number 1-6, 7-12, 13-18, and 19-24, respectively. The higher the leaf maturity, the higher the chlorophyll content. However, the over mature leaves were only slightly different from the mature ones. In addition, pandan leaves have specific flavor and contain carotenoid, phenolic, and flavonoid substances. Anatomically, the mesophyll’s size was greatest in the mature leaves, while the size of chloroplast was not significantly different from medium to over mature leaves. Based on the chlorophyll content and mesophyll size, it was concluded that mature pandan leaves were the best source of chlorophyll, containing chlorophyll of 623.08 mg/100 g dry weight (DW).


HortScience ◽  
2010 ◽  
Vol 45 (9) ◽  
pp. 1378-1383 ◽  
Author(s):  
Youbin Zheng ◽  
Diane Feliciano Cayanan ◽  
Mike Dixon

To determine the optimum feeding nutrient solution concentrations for the production of potted miniature roses (Rosa chineersis minima ‘Fall Festival’) under recirculating subirrigation conditions, plants were grown under four different nutrient solution concentrations [25%, 50%, 75%, and 100% of the full strength with an electrical conductivity (EC) of 1.756 dS·m−1]. Nutrient solution concentrations affected the stem, root, and plant total dry weight and flower and branch number. Under the 75% strength nutrient solution, these growth parameters were equal to or better than the 100% strength solution. No difference was detected in the chlorophyll content of leaves from plants that received the 50%, 75%, and 100% strength solutions during the experiment but at Day 35; only the 25% treatment had significantly lower leaf chlorophyll content than the other treatments. There were no treatment effects on the measured total foliar nutrient contents [except potassium (K)] of plants under the 75% strength solution compared with those under the 100% treatment, but nitrogen (N), phosphorus (P), and/or iron (Fe) of plants under the 25% strength solutions were below that of the acceptable range. Interveinal chlorosis and/or reddish leaves and branches were also apparent in plants under the 25% and 50% strength solutions. It is suspected that these are symptoms of N, P, and Fe deficiencies caused by the reduced nutrient solution concentrations and increased pH of the growing substrate. There were significant depletions of N and P nutrients in the 25% and 50% strength solutions at the end of the experiment, which was consistent with visual symptoms and deficiencies. Nutrient salts accumulated in the top section of the growing substrate under all treatments, but no phytotoxic effects were observed. The EC values for the top third of the growing substrate were much higher than those of the bottom two-thirds. EC for the top layer of the 100% treatment exhibited a fourfold increase compared with the bottom layer of that treatment. The NO3–, K, magnesium, and calcium for the top layer of the 100% treatment were 235%, 149%, 287%, and 245%, respectively, higher compared with the bottom layer of the 100% treatment. It was concluded that the nutrient solution concentrations typically used for potted miniature rose production in most of the Canadian greenhouses under recirculating subirrigation conditions can be safely reduced to 75% and produce better plants.


Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1299
Author(s):  
Luca Regni ◽  
Daniele Del Buono ◽  
Begoña Miras-Moreno ◽  
Biancamaria Senizza ◽  
Luigi Lucini ◽  
...  

Biostimulants are becoming increasingly popular in agriculture for their ability to induce beneficial effects in crops, paving the way towards the identification of new materials with biostimulant potential. This study evaluated the potential of different concentrations of an aqueous extract (0.25%, 0.50%, and 1.00%, dry weight/water volume, respectively) obtained from duckweed (Lemna minor L.) to stimulate olive plants. Leaf net photosynthesis (Pn), leaf transpiration rate (E), stomatal conductance (gs), sub-stomatal CO2 concentration (Ci), chlorophyll content and other plant growth parameters were investigated. As a result, the extract improved Pn, gs, Ci, chlorophyll content and plant biomass production (leaf fresh and dry weight). Furthermore, the duckweed extract generally increased the uptake of nitrogen (N), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe) and zinc (Zn), while it did not influence the content of sodium (Na), manganese (Mn) and copper (Cu). The untargeted metabolomic profiling of the extract revealed the presence of signalling compounds (including phytohormones), phenolics and glutathione. Such broad diversity of bioactives may support the stimulatory potential observed in olive. In summary, this study revealed for the first time that duckweed could be seen as a promising species to obtain extracts with biostimulant properties in olive trees.


2020 ◽  
Vol 8 (2) ◽  
pp. 40-53
Author(s):  
Basel Natsheh ◽  
Nawaf Abu-Khalaf

The experiment was conducted during the period from March to the end of April 2019 to observe the effect of different fertilizer regimes on the growth, quality and yield of lettuce under field conditions. The experiment was consisting of four treatments as well as a control without fertilizer T1, compost T2, compost tea T3 and chemical fertilizer T4, in each treatment 50 replicates were planted. The measurements included wet and dry weight for shoot and root, plant length, and chlorophyll percentage. The study revealed that lettuce's best growth values were obtained with the use of eco-friendly organic fertilizer accompanied by more than control one and chemical fertilizer. The average maximum length of plants after 60 days were recorded in T2 (42.3 cm) > T3 (37.3 cm), T4 (35.9 cm) and > T1 (23.8 cm). The results indicated that compost treated plant (T2 and tea compost T3) had highest chlorophyll content, i.e. 42.3 and 40.6%, respectively, as compared to chemical fertilizers T4 (37.3%) and the lowest chlorophyll content was in control T1 (30.9%). The values for wet shoot and root after 60 days were: 1575.2, 81.5 gm T2 > 1367.6, 64.9 gm T3 > 1251.1, 53.4 gm T4 and 612.1, 43.7 gm T1, respectively. The values for dry shoot and root after 60 days were: 131.5, 8.2 gm, T2 > 112.7, 6.4 gm, T3 > 101.9, 5.3 gm T4 and > 51, 4.1 gm T1, respectively.


2015 ◽  
Vol 55 (2) ◽  
pp. 126-135
Author(s):  
Magd El-Morsi Awad El-Morsi ◽  
Montaser Fawzy Abdel-Monaim

Abstract Root rot and wilt disease complex was detected in several fig (Ficus carica L.), grapevine (Vitis vinifera L.), and pomegranate (Punica granatum L.) transplants in nurseries and new orchards of the El-Kharga, Baris, Balate, El-Dakhla, and El-Farafrah districts, of the New Valley governorate, Egypt. The percentage of root rot/wilt incidence and severity on fig, grapevine, and pomegranate transplants in the surveyed districts differed. The average percentages of root rot/wilt incidence and severity, in the surveyed districts, were 41.26, 31.42% in fig, 38.2, 29.5% in grapevine, and 32.1, 23.7% in pomegranate transplants, respectively. The most frequently isolated fungi from rotted roots of fig, grapevine, and pomegranate transplants were Fusarium oxysporum, Rhizoctonia solani, and Macrophomena phaseolina. In pathogenicity tests, all the tested fungi were pathogenic to fig, grapevine, and pomegranate transplants. Under laboratory conditions, all of the following tested bio-agents: Azotobacter sp., Bacillus cereus, B. megaterium, and B. subtilis, were able to inhibit growth of the causal pathogens to different degrees. The effect of these bio-agents individually and/or mixed, when used as a soil drench treatment, were varied in reducing the incidence and severity of root rot/wilt diseases in fig, grapevine, and pomegranate transplants under greenhouse conditions. The mixed bio-agents gave the highest protection against root rot/wilt diseases compared with the individually used of bio-agents. All treatments significantly increased plant height, number of leaves/transplant, leaf area, fresh and dry weight/transplant compared with the control treatment.


2016 ◽  
Vol 19 (s1) ◽  
pp. 35-39 ◽  
Author(s):  
Andrea Tilly-Mándy ◽  
Anna Radó-Takács ◽  
Z. Rab ◽  
Péter Honfi

AbstractThree kinds of recently developed plant growing media and organic fertilizers were studied separately or in combinations inTagetes patulaL. ‘Csemő’ production at the Szent István University, Budapest in 2014. Plant height and width; fresh weight and fresh/dry weight rate, chlorophyll content and peroxidase enzyme activity were detected. Highest and widest plants were obtained in BRT®GreenMoss, GreenMoss in combination with 10% BRT®Evergreen and in common growing mixture combined with 30% BRT®Evergreen in combination with Fainsoil Bioactivator treatment. All treatments increased fresh weight and chlorophyll content.


2017 ◽  
Vol 30 (2) ◽  
pp. 72-82
Author(s):  
Abdulnabi A.A. Matrood ◽  
Azher H. Al-Taie

The agricultural production processes currently targeted reducing chemical fungicides usage and increasing bio-agent application through controlling diseases alone or integrating it with other factors. The study aimed to investigate the induction of systemic resistance by multi bio-agents represented by mycorrhizal fungi Glomus mosseae, G. intradicas and Trichoderma harizanum against pathogenic fungus Rhizoctonia solani which caused wilt disease and growth defoliation to Okra seedling. Three isolate of R. solani were recorded on root of Okra seedling, named (local - Batra). Isolate no. (3) was more virulence than other isolates in damping off disease in the pre and post emergence. Results also showed that G. mosseae and G. intradicas with T. harizanum had a positive influence in reducing detrimental effect of R. solani in all growth parameters (e.g. fresh and dry weight of root) on disease severity on Okra plant caused by R. solani. Bio-agents (G. mosseae,G. intradicas and T. harizanum) increased resistance in Okra plants by raising production of enzymescatalase and Peroxidase.this experiment was revealed that using a complex of bio-agent’s factors were greatly increase the efficiency of biological control than using each of them individually. We conclude that the broad diversity of rhizosphere micro-organisms as well as the confronting between the bio-chemical and physical changes could be reflected the variations in the metabolic secondary products that could inhibit pathogens.


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