scholarly journals Characterization of Bordetella Bronchiseptica Isolated from Rabbits in Fujian, China

2020 ◽  
Author(s):  
Jinxiang Wang ◽  
Shikun Sun ◽  
Yanfeng Chen ◽  
Dongjin Chen ◽  
Lei Sang ◽  
...  
Keyword(s):  
Respiratory Disease ◽  
Animal Species ◽  
Bordetella Bronchiseptica ◽  
Economic Losses ◽  
Fujian Province ◽  
Pcr Screening ◽  
Resistance Rates ◽  
First Time ◽  
Sequence Types

Abstract Background Bordetella bronchiseptica can infect many animal species, and is a potential zoonotic pathogen that can also infect humans. In rabbits, infection of B. bronchiseptica is associated with respiratory disease, which causes economic losses to the rabbit farming. Fujian Province is a traditional importance rabbit farming area in China. However, no literature about the epidemiology and characteristics of B. bronchiseptica in rabbits in Fujian Province has been reported.Results A total of 219 B. bronchiseptica isolates were recovered from the 833 lung samples of dead rabbits with respiratory disease. The 219 isolates were typed into 11 sequence types (STs) including 5 known STs (ST6, ST10, ST12, ST14 and ST33) and 6 new STs (ST88, ST89, ST90, ST91, ST92 and ST93) by using multi-locus sequence typing (MLST). Surprisingly, all the 219 isolates carried the 5 virulence genes of fhaB, prn, cyaA, dnt and bteA in the PCR screening. Moreover, the isolates resistance to cefixime, ceftizoxime, cefatriaxone and ampicillin were detected, and the resistance rates to the 4 kinds of drug were 33.33, 31.05, 11.87 and 3.20%, respectively.Conclusions In the present study, we showed for the first time that B. bronchiseptica is widespread in rabbits in Fujian Province, and that B. bronchiseptica is an important pathogen associating with respiratory disease in rabbits in Fujian Province. Moreover, it should be alert to the potential occurrence of transmission events between rabbits and humans because the B. bronchiseptica strain of ST12 that can infect humans were also isolated from rabbits in Fujian Province.

scholarly journals Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China

2019 ◽  
Author(s):  
Jinxiang Wang ◽  
Lei Sang ◽  
Shikun Sun ◽  
Yanfeng Chen ◽  
Dongjin Chen ◽  
...  
Keyword(s):  
Respiratory Disease ◽  
Pasteurella Multocida ◽  
Sequence Type ◽  
Economic Losses ◽  
Pcr Screening ◽  
Resistance Rates ◽  
And Control ◽  
First Time ◽  
Sequence Types

Abstract Background: Pasteurella multocida is one of the important pathogens that infect rabbits, causing major economic losses in commercial rabbit farming. In this study, 205 P. multocida isolates recovered from lungs of dead rabbits with respiratory disease were defined by capsular serogroups, lipopolysaccharide (LPS) genotypes and multi-locus sequence types, screened virulence factors and antimicrobial susceptibility. Results: The 205 isolates were assigned into 2 capsular types, A and D, and 2 LPS genotypes, L3 and L6. When combining capsular types with LPS genotypes, 4 serotypes were detected. A:L3 (51.22%, 105/205) was the most predominant serotype, followed by A:L6 (24.88%, 51/205), D:L6 (19.02%, 39/205) and D:L3 (4.88%, 10/205). The 205 isolates were grouped into 3 sequence types, ST10, ST11 and ST12. ST12 (56.10%, 115/205) was the most prevalent sequence type, followed by ST10 (24.88%, 51/205) and ST11 (19.02%, 39/205). In the 205 isolates, virulence associated genes ptfA , fur , hgbB , ompA , ompH and oma87 were positive in the PCR screening, whereas the toxA and tbpA genes were negative. Notably, the 156 capsular serogroup A isolates carried the pmHAS gene. All the 205 isolates were susceptible to most of the used antibiotics, except for streptomycin, gentamycin, kanamycin and ceftriaxone, and the resistance rates of which were 27.80%, 15.61%, 9.27% and 2.44%, respectively. Conclusions: This study, for the first time, described the prevalence and characteristics of P. multocida causing respiratory disease in rabbits in Fujian Province, which might be useful for tracking the epidemic strains and development of efficient vaccines and methods to prevent and control the pathogen.

scholarly journals Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China

2019 ◽  
Author(s):  
Jinxiang Wang ◽  
Lei Sang ◽  
Shikun Sun ◽  
Yanfeng Chen ◽  
Dongjin Chen ◽  
...  
Keyword(s):  
Respiratory Disease ◽  
Pasteurella Multocida ◽  
Sequence Type ◽  
Economic Losses ◽  
Pcr Screening ◽  
Resistance Rates ◽  
And Control ◽  
First Time ◽  
Sequence Types

Abstract Background: Pasteurella multocida is one of the important pathogens that infect rabbits, causing major economic losses in commercial rabbit farming. In this study, 205 P. multocida isolates recovered from lungs of dead rabbits with respiratory disease were defined by capsular serogroups, lipopolysaccharide (LPS) genotypes, multi-locus sequence types and screened virulence factors by using PCR assays, and tested antimicrobial susceptibility. Results: The 205 isolates were assigned into 2 capsular types, A and D, and 2 LPS genotypes, L3 and L6. When combining capsular types with LPS genotypes, 4 serotypes were detected. A:L3 (51.22%, 105/205) was the most predominant serotype, followed by A:L6 (24.88%, 51/205), D:L6 (19.02%, 39/205) and D:L3 (4.88%, 10/205). The 205 isolates were grouped into 3 sequence types, ST10, ST11 and ST12. ST12 (56.10%, 115/205) was the most prevalent sequence type, followed by ST10 (24.88%, 51/205) and ST11 (19.02%, 39/205). In the 205 isolates, virulence associated genes ptfA, fur, hgbB, ompA, ompH and oma87 were positive in the PCR screening, whereas the toxA and tbpA genes were negative. Notably, the 156 capsular serogroup A isolates carried the pmHAS gene. All the 205 isolates were susceptible to most of the used antibiotics, except for streptomycin, gentamycin, kanamycin and ceftriaxone, and the resistance rates of which were 27.80%, 15.61%, 9.27% and 2.44%, respectively. Conclusions: This study, for the first time, described the prevalence and characteristics of P. multocida causing respiratory disease in rabbits in Fujian Province, which might be useful for tracking the epidemic strains and development of efficient vaccines and methods to prevent and control the pathogen.

scholarly journals Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Jinxiang Wang ◽  
Lei Sang ◽  
Shikun Sun ◽  
Yanfeng Chen ◽  
Dongjin Chen ◽  
...  
Keyword(s):  
Respiratory Disease ◽  
Pasteurella Multocida ◽  
Sequence Type ◽  
Economic Losses ◽  
Pcr Screening ◽  
Resistance Rates ◽  
And Control ◽  
First Time ◽  
Sequence Types

Abstract Background Pasteurella multocida is one of the important pathogens that infect rabbits, causing major economic losses in commercial rabbit farming. In this study, 205 P. multocida isolates recovered from lungs of dead rabbits with respiratory disease were defined by capsular serogroups, lipopolysaccharide (LPS) genotypes, multi-locus sequence types and screened virulence factors by using PCR assays, and tested antimicrobial susceptibility. Results The 205 isolates were assigned into 2 capsular types, A and D, and 2 LPS genotypes, L3 and L6. When combining capsular types with LPS genotypes, 4 serotypes were detected. A:L3 (51.22%, 105/205) was the most predominant serotype, followed by A:L6 (24.88%, 51/205), D:L6 (19.02%, 39/205) and D:L3 (4.88%, 10/205). The 205 isolates were grouped into 3 sequence types, ST10, ST11 and ST12. ST12 (56.10%, 115/205) was the most prevalent sequence type, followed by ST10 (24.88%, 51/205) and ST11 (19.02%, 39/205). In the 205 isolates, virulence associated genes ptfA, fur, hgbB, ompA, ompH and oma87 were positive in the PCR screening, whereas the toxA and tbpA genes were negative. Notably, the 156 capsular serogroup A isolates carried the pmHAS gene. All the 205 isolates were susceptible to most of the used antibiotics, except for streptomycin, gentamycin, kanamycin and ceftriaxone, and the resistance rates of which were 27.80, 15.61, 9.27 and 2.44%, respectively. Conclusions This study, for the first time, described the prevalence and characteristics of P. multocida causing respiratory disease in rabbits in Fujian Province, which might be useful for tracking the epidemic strains and development of efficient vaccines and methods to prevent and control the pathogen.

scholarly journals Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China

2019 ◽  
Author(s):  
Jinxiang Wang ◽  
Lei Sang ◽  
Shikun Sun ◽  
Yanfeng Chen ◽  
Dongjin Chen ◽  
...  
Keyword(s):  
Respiratory Disease ◽  
Pasteurella Multocida ◽  
Virulence Genes ◽  
Sequence Type ◽  
Economic Losses ◽  
Resistance Rates ◽  
And Control ◽  
First Time ◽  
Sequence Types

Abstract Background Pasteurella multocida is one of the important pathogens infects rabbits, causing major economic losses in commercial rabbit farming. In this study, 205 P. multocida isolates recovered from lungs of dead rabbits with respiratory disease were defined capsular serogroups, lipopolysaccharide (LPS) genotypes and multi-locus sequence types, screened virulence factors, and tested antimicrobial susceptibility. Results The 205 isolates were assigned into 2 capsular types, A and D, and 2 LPS genotypes, L3 and L6. When combining capsular types with LPS genotypes, 4 serotypes were detected. A:L3 (51.22%, 105/205) was the most predominant serotype, followed by A:L6 (24.88%, 51/205), D:L6 (19.02%, 39/205), and D:L3 (4.88%, 10/205). The 205 isolates were grouped into 3 sequence types, ST10, ST11 and ST12. ST12 (56.10%, 115/205) was the most prevalent sequence type, followed by ST10 (24.88%, 51/205) and ST11 (19.02%, 39/205). In the 205 isolates, the virulence genes of ptfA, fur, hgbB, ompA, ompH and oma87 were positive, whereas the toxA and tbpA genes were negative. Notably, the 156 capsular serogroup A isolates carried pmHAS gene. All the 205 isolates were susceptible to most of the used antibiotics, except for streptomycin, gentamycin, kanamycin and ceftriaxone, and the resistance rates of which were 27.80%, 15.61%, 9.27% and 2.44%, respectively. Conclusions This study, for the first time, described the prevalence and characteristics of P. multocida causing respiratory disease in rabbits in Fujian Province, which might be useful for tracking the epidemic strains and development of efficient vaccines and methods to prevent and control the pathogen.

scholarly journals Characterisation of Bordetella bronchiseptica isolated from rabbits in Fujian, China

2020 ◽  
Vol 148 ◽  
Author(s):  
J. Wang ◽  
S. Sun ◽  
Y. Chen ◽  
D. Chen ◽  
L. Sang ◽  
...  
Keyword(s):  
Respiratory Diseases ◽  
Animal Species ◽  
Virulence Genes ◽  
Bordetella Bronchiseptica ◽  
Chain Reaction ◽  
Fujian Province ◽  
Zoonotic Pathogen ◽  
Polymerase Chain ◽  
Preventive Methods ◽  
Sequence Types

Abstract Bordetella bronchiseptica is a potential zoonotic pathogen, which mainly causes respiratory diseases in humans and a variety of animal species. B. bronchiseptica is one of the important pathogens isolated from rabbits in Fujian Province. However, the knowledge of the epidemiology and characteristics of the B. bronchiseptica in rabbits in Fujian Province is largely unknown. In this study, 219 B. bronchiseptica isolates recovered from lung samples of dead rabbits with respiratory diseases in Fujian Province were characterised by multi-locus sequencing typing, screening virulence genes and testing antimicrobial susceptibility. The results showed that the 219 isolates were typed into 11 sequence types (STs) including five known STs (ST6, ST10, ST12, ST14 and ST33) and six new STs (ST88, ST89, ST90, ST91, ST92 and ST93) and the ST33 (30.14%, 66/219), ST14 (26.94%, 59/219) and ST12 (16.44%, 36/219) were the three most prevalent STs. Surprisingly, all the 219 isolates carried the five virulence genes (fhaB, prn, cyaA, dnt and bteA) in the polymerase chain reaction screening. Moreover, the isolates were resistant to cefixime, ceftizoxime, cefatriaxone and ampicillin at rates of 33.33%, 31.05%, 11.87% and 3.20%, respectively. This study showed the genetic diversity of B. bronchiseptica in rabbits in Fujian Province, and the colonisation of the human-associated ST12 strain in rabbits in Fujian Province. The results might be useful for monitoring the epidemic strains, developing preventive methods and preventing the transmission of epidemic strains from rabbits to humans.

scholarly journals Genetic Characterization of a Novel Mutant of Peste Des Petits Ruminants Virus Isolated fromCapra ibexin China during 2015

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Zixiang Zhu ◽  
Xiaocui Zhang ◽  
Gulizhati Adili ◽  
Jiong Huang ◽  
Xiaoli Du ◽  
...  
Keyword(s):  
Genomic Sequences ◽  
Economic Losses ◽  
N Gene ◽  
Peste Des Petits Ruminants ◽  
Capra Ibex ◽  
V Protein ◽  
First Time ◽  
Lineage Iv ◽  
New Mutations

Peste des petits ruminants virus (PPRV) is the causative agent of peste des petits ruminants (PPR). The spread of PPR often causes severe economic losses. Therefore, special attention should be paid to the surveillance of PPR emergence, spread, and geographic distribution. Here we describe a novel mutant of PPRV China/XJBZ/2015 that was isolated fromCapra ibexin Xinjiang province in China 2015. The sequence analysis and phylogenetic assessment indicate that China/XJBZ/2015 belongs to lineage IV, being closely related to China/XJYL/2013 strain. Interestingly, the V protein sequence of China/XJBZ/2015 showed lower homology with other Chinese PPRVs isolated during 2013 to 2014 (94%~95%), whereas it shared 100% identity with three Tibet strains isolated in China 2007. The 3′ UTR, V gene, and C gene were determined to be highly variable. Besides, 29 PPR genomic sequences available in GenBank were analyzed in this study. It is the first time to use PPRV genomic sequences to classify the different lineages which confirmed the lineage clustering of PPRVs using N gene 255 bp fragments and F gene 322 bp fragments. In conclusion, our findings indicate that the PPRVs continue to evolve in China, and some new mutations have emerged.

scholarly journals Comparative Genomic Analysis and Phenotypic Characterization of Bronchoscope-Associated Klebsiella aerogenes

2021 ◽  
Vol 70 (3) ◽  
pp. 409-412
Author(s):  
FANG HUANG ◽  
SHUANG LI ◽  
LAN LOU ◽  
JUNJUN MO ◽  
HAO XU
Keyword(s):  
Antimicrobial Agents ◽  
Genomic Analysis ◽  
Phenotypic Characterization ◽  
Comparative Genomic ◽  
Klebsiella Aerogenes ◽  
Clinical Procedures ◽  
First Time ◽  
Sequence Types ◽  
Level Resistance

Bronchoscopes have been linked to outbreaks of nosocomial infections. The phenotypic and genomic profiles of bronchoscope-associated Klebsiella aerogenes isolates are largely unknown. In this work, a total of 358 isolates and 13 isolates were recovered from samples after clinical procedures and samples after decontamination procedures, respectively, over the five months. Antimicrobial susceptibility testing found seven K. aerogenes isolates exhibiting a low-level resistance to antimicrobial agents. Among seven K. aerogenes isolates, we found five sequence types (STs) clustered into three main clades. Collectively, this study described for the first time the phenotypic and genomic characteristics of bronchoscope-associated K. aerogenes.

scholarly journals Characterization of community and hospital Staphylococcus aureus isolates in Southampton, UK

2010 ◽  
Vol 59 (9) ◽  
pp. 1084-1088 ◽  
Author(s):  
S. M. Green ◽  
P. Marsh ◽  
N. Ahmad ◽  
J. M. C. Jefferies ◽  
S. C. Clarke
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Analysis ◽  
Community Settings ◽  
Type Iv ◽  
Mrsa Strains ◽  
The Uk ◽  
Healthcare Associated ◽  
First Time ◽  
Sequence Types

Staphylococcus aureus infections are a burden to healthcare systems. There remains a lack of understanding on the relative contributions of S. aureus infection in the healthcare and community settings. In this study, 59 S. aureus isolates were selected for molecular analysis. The mobile variant staphylococcal cassette chromosome mec type IV was present in both healthcare-associated meticillin-resistant S. aureus (HA-MRSA) and community-associated MRSA (CA-MRSA), as was the Panton–Valentine leukocidin gene. PFGE identified 24 distinct clonal groups whilst multi-locus sequence typing identified 26 different sequence types, including four with new combinations of alleles. This is the first time, to our knowledge, that a selection of CA and HA MSSA and MRSA strains have been subjected to molecular analysis and comparison in the UK. Definitions for CA-MRSA need further debate as the movement of strains between healthcare and community settings is confounding the use of epidemiological definitions.

scholarly journals Characterisation of Staphylococcus aureus isolated from rabbits in Fujian, China

2019 ◽  
Vol 147 ◽  
Author(s):  
J. Wang ◽  
L. Sang ◽  
S. Sun ◽  
Y. Chen ◽  
D. Chen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Virulence Factors ◽  
Antimicrobial Susceptibility ◽  
Virulence Genes ◽  
Susceptibility Test ◽  
Antimicrobial Susceptibility Test ◽  
Fujian Province ◽  
Genetic Characteristics ◽  
Resistance Rates ◽  
Sequence Types

Abstract Staphylococcus aureus has been recognised as one of the important zoonotic pathogens. However, knowledge about the epidemiology and genetic characteristics of S. aureus in rabbits was limited. The aim of this study was to determine the characteristics of 281 S. aureus isolated from dead rabbits of nine rabbit farms in Fujian Province, China. All the isolates were characterised by multi-locus sequencing typing, detection of virulence factors and antimicrobial susceptibility test. The results showed that the 281 isolates were grouped into two sequence types, ST121 (13.52%, 38/281) and ST398 (86.48%, 243/281). Surprisingly, the ST121 strains were only recovered from the lung samples from one of the nine rabbit farms studied. In the 281 isolates, the virulence genes of nuc, hla, hlb, clfA, clfB and fnbpA were positive, whereas the sea, seb, tsst, eta and etb genes were negative. Notably, the 38 ST121 isolates carried the pvl gene. All the 281 isolates were methicillin-susceptible S. aureus, and the isolates were susceptible to most of the used antibiotics, except for streptomycin, kanamycin, azithromycin and penicillin, and the resistance rates of which were 23.84%, 19.57%, 16.01% and 11.03%, respectively. This study first described the epidemiology and characteristics of S. aureus in rabbits in Fujian Province, which will help in tracking the evolution of epidemic strains and preventing the rabbit–human transmission events.

scholarly journals High β-Lactam and Quinolone Resistance of Enterobacteriaceae from the Respiratory Tract of Sheep and Goat with Respiratory Disease

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2258
Author(s):  
Hazim O. Khalifa ◽  
Atef Oreiby ◽  
Amer Ali Abd El-Hafeez ◽  
Amira Abd El Latif ◽  
Takashi Okanda ◽  
...  
Keyword(s):  
Respiratory Disease ◽  
Multidrug Resistant ◽  
Resistance Mechanisms ◽  
Quinolone Resistance ◽  
Resistant Bacteria ◽  
Aminoglycoside Resistance ◽  
Gram Negative ◽  
Sheep And Goats ◽  
Resistance Rates ◽  
First Time

During the last decade’s increase of antimicrobial resistance (AMR) in animals, animal-human transmission has become a major threat. Therefore, the present study aimed to evaluate the genetic basis of AMR in Gram-negative bacteria recovered from sheep and goats with respiratory disease. Nasal and ocular swabs were collected from 69 diseased animals, and 76 Gram-negative bacterial isolates were identified from 59 animals. All isolates were checked phenotypically for resistance and genotypically for different resistance mechanisms, including β-lactam, quinolone, and aminoglycoside resistance. Our results demonstrated that 9.2% (95% CI 4.5–17.8%) of the isolates were multidrug-resistant, with high resistance rates to β-lactams and quinolones, and 11.8% (95% CI 6.4–21%) and 6.6% (95% CI 2.8–14.5%) of the isolates were phenotypically positive for AmpC and ESBL, respectively. Genotypically, blaTEM was the most identified β-lactamase encoding gene in 29% (95% CI 20–40%) of the isolates, followed by blaSHV (14.5%, 95% CI 8.3–24.1%) and blaCTX-M (4%, 95% CI 1.4–11%). Furthermore, 7.9% (95% CI 3.7–16.2%) of the isolates harbored plasmid-mediated quinolone resistance gene qnrS. Our study revealed for the first time to our knowledge high β-lactam and quinolone resistance associated with the bacteria recovered from sheep and one goat with respiratory disease. Furthermore, different antimicrobial resistant determinants were identified for the first time from animals in Africa, such as blaLEN-13/55, blaTEM-176 and blaTEM-198/214. This study highlights the potential role of sheep and goats in disseminating AMR determinants and/or resistant bacteria to humans. The study regenerates interest for the development of a One Health approach to combat this formidable problem.

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