Pre-analytical sampling and storage conditions of Amyloid-β peptides in venous and capillary blood

2020 ◽  
Author(s):  
Marlena Walter ◽  
Jens Wiltfang ◽  
Jonathan Vogelgsang
Keyword(s):  
Whole Blood ◽  
Room Temperature ◽  
Amyloid Β ◽  
Storage Conditions ◽  
Capillary Blood ◽  
Research Practice ◽  
Aβ Peptides ◽  
Standard Operating ◽  
The Impact ◽  
And Storage

Abstract BACKGROUND: We analyzed the impact of pre-analytical storage and sampling conditions on plasma Amyloid-β (Aβ) peptides.METHODS: Venous or capillary blood was collected and either stored as plasma or whole blood at room temperature or 4°C. Aβ 40 and Aβ 42 levels were measured using a chemiluminescence sandwich immunoassay.RESULTS: Aβ 40 , Aβ 42 and Aβ 42/40 levels significantly decreased during storage at room temperature in whole blood or plasma, starting at 6 hours after sampling. While Aβ 42 was most prone to the time of storage, Aβ 42/40 was stable up to 24 hours. Storing whole blood or plasma at 4°C led to stable Aβ peptide concentrations up to 72 hours. In addition, Aβ peptides could be measured in capillary blood. While Aβ 40 and Aβ 42 concentrations were slightly lower in capillary blood and started decreasing during storage conditions at 4°C, the Aβ 42/40 ratio remained constant up to 72 hours and was comparable to venous whole blood.CONCLUSION: These findings provide information that need to be respected in pre-analytical standard operating procedures for clinical and research practice to generate most reliable plasma Aβ measurements.

scholarly journals Pre-Analytical Sampling and Storage Conditions of Amyloid-β Peptides in Venous and Capillary Blood

2020 ◽  
Vol 78 (2) ◽  
pp. 529-535
Author(s):  
Marlena Walter ◽  
Jens Wiltfang ◽  
Jonathan Vogelgsang
Keyword(s):  
Blood Test ◽  
Room Temperature ◽  
Screening Tool ◽  
Amyloid Β ◽  
Storage Conditions ◽  
Capillary Blood ◽  
Sample Storage ◽  
Aβ Peptides ◽  
Blood Samples ◽  
And Storage

Previous studies on blood-based biomarkers for Alzheimer’s disease suggest a less invasive blood test might be a valuable screening tool for Alzheimer-specific pathology. Pre-analytical sample storage conditions seem to play an important role on amyloid-β (Aβ) stability, impacting reliability and reproducibility. This study shows that Aβ40, Aβ42, and Aβ42/40 levels significantly and early decrease during storage at room temperature in whole blood or plasma. Storing blood samples at 4°C leads to stable Aβ peptide concentrations up to 72 h. In addition, Aβ peptides can be measured in capillary blood with a stable Aβ42/40 ratio up to 72 h at 4°C.

scholarly journals Impact of Storage Conditions on the Methanogenic Activity of Anaerobic Digestion Inocula

Water ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1321 ◽  
Author(s):  
Sergi Astals ◽  
Konrad Koch ◽  
Sören Weinrich ◽  
Sasha D. Hafner ◽  
Stephan Tait ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Room Temperature ◽  
Storage Time ◽  
Storage Temperature ◽  
Storage Conditions ◽  
Methanogenic Activity ◽  
Specific Methanogenic Activity ◽  
The Impact ◽  
And Storage ◽  
Over Time

The impact of storage temperature (4, 22 and 37 °C) and storage time (7, 14 and 21 days) on anaerobic digestion inocula was investigated through specific methanogenic activity assays. Experimental results showed that methanogenic activity decreased over time with storage, regardless of storage temperature. However, the rate at which the methanogenic activity decreased was two and five times slower at 4 °C than at 22 and 37 °C, respectively. The inoculum stored at 4 °C and room temperature (22 °C) maintained methanogenic activity close to that of fresh inoculum for 14 days (<10% difference). However, a storage temperature of 4 °C is preferred because of the slower decrease in activity with lengthier storage time. From this research, it was concluded that inoculum storage time should generally be kept to a minimum, but that storage at 4 °C could help maintain methanogenic activity for longer.

Should phosphatidylethanol be currently analysed using whole blood, dried blood spots or both?

2019 ◽  
Vol 57 (5) ◽  
pp. 617-622 ◽  
Author(s):  
Van Long Nguyen ◽  
Michael Fitzpatrick
Keyword(s):  
Whole Blood ◽  
Blood Cells ◽  
Dried Blood Spots ◽  
Storage Conditions ◽  
Ethanol Metabolism ◽  
Clinical Use ◽  
Blood Spots ◽  
Comparative Review ◽  
And Storage

Abstract Phosphatidylethanol (PEth) are phospholipids produced through non-oxidative ethanol metabolism. They accumulate in red blood cells and have been traditionally analysed in whole blood as potential biomarkers for moderate to long-term alcohol consumption. More recently, their analysis in dried blood spots has been gaining favour, namely, due to the ease in sampling, transport and storage conditions required. This paper aims at providing a short comparative review between analysing PEth in whole blood and dried blood spots and the potential pitfalls that researchers may face when setting up PEth testing for clinical use.

scholarly journals The Impact of Cold Storage on Adenosine Diphosphate-Mediated Platelet Responsiveness

TH Open ◽  
2020 ◽  
Vol 04 (03) ◽  
pp. e163-e172
Author(s):  
Juergen Koessler ◽  
Philipp Klingler ◽  
Marius Niklaus ◽  
Katja Weber ◽  
Angela Koessler ◽  
...  
Keyword(s):  
Cold Storage ◽  
Whole Blood ◽  
Room Temperature ◽  
Purinergic Receptor ◽  
Adenosine Diphosphate ◽  
Receptor Expression ◽  
Activation Markers ◽  
Inhibitory Pathways ◽  
The Impact ◽  
Induced Aggregation

Abstract Introduction Cold storage of platelets is considered to contribute to lower risk of bacterial growth and to more efficient hemostatic capacity. For the optimization of storage strategies, it is required to further elucidate the influence of refrigeration on platelet integrity. This study focused on adenosine diphosphate (ADP)-related platelet responsiveness. Materials and Methods Platelets were prepared from apheresis-derived platelet concentrates or from peripheral whole blood, stored either at room temperature or at 4°C. ADP-induced aggregation was tested with light transmission. Activation markers, purinergic receptor expression, and P2Y12 receptor function were determined by flow cytometry. P2Y1 and P2X1 function was assessed by fluorescence assays, cyclic nucleotide concentrations by immunoassays, and vasodilator-stimulated phosphoprotein (VASP)-phosphorylation levels by Western blot analysis. Results In contrast to room temperature, ADP-induced aggregation was maintained under cold storage for 6 days, associated with elevated activation markers like fibrinogen binding or CD62P expression. Purinergic receptor expression was not essentially different, whereas P2Y1 function deteriorated rapidly at cold storage, but not P2Y12 activity. Inhibitory pathways of cold-stored platelets were characterized by reduced responses to nitric oxide and prostaglandin E1. Refrigeration of citrated whole blood also led to the attenuation of induced inhibition of platelet aggregation, detectable within 24 hours. Conclusion ADP responsiveness is preserved under cold storage for 6 days due to stable P2Y12 activity and concomitant disintegration of inhibitory pathways enabling a higher reactivity of stored platelets. The ideal storage time at cold temperature for the highest hemostatic effect of platelets should be evaluated in further studies.

Impact of Alloantigens and Storage-associated Factors on Stimulated Cytokine Response in an In Vitro  Model of Blood Transfusion

2002 ◽  
Vol 97 (5) ◽  
pp. 1102-1109 ◽  
Author(s):  
Andreas E. Biedler ◽  
Sven O. Schneider ◽  
Ullrich Seyfert ◽  
Hauke Rensing ◽  
Sasha Grenner ◽  
...  
Keyword(s):  
Whole Blood ◽  
Mononuclear Cells ◽  
Autologous Blood ◽  
Immunosuppressive Effect ◽  
Tnf Alpha ◽  
Major Surgery ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fresh Blood ◽  
The Impact ◽  
And Storage

Background Transfusion of blood may contribute to immunosuppression in major surgery. The authors assessed the impact of alloantigens and storage on function of peripheral blood mononuclear cells cultured in their physiologic environment. Methods Blood units (whole blood, packed erythrocytes) were prepared with or without prestorage leukodepletion and stored for 24-26 days. Blood samples were coincubated with allogeneic fresh blood, autologous, or allogeneic stored blood. Endotoxin-stimulated release of tumor necrosis factor-alpha (TNF-alpha) and interleukin 10 (IL-10) was measured after 24 h of culture by enzyme-linked immunosorbent assay. Results Coincubation with equal amounts of allogeneic fresh blood showed almost no influence on TNF-alpha (-12%, not significant) and IL-10 (+11%, not significant) release. Stored allogeneic whole blood resulted in a significant TNF-alpha depression (-61%) and IL-10 induction (+221%). These effects were diminished but not prevented by prestorage leukodepletion (TNF-alpha -42%, IL-10 +110%) and required the presence of soluble factors (TNF-alpha suppression) and cellular components (IL-10 induction). TNF-alpha decrease and IL-10 increase were in the same order of magnitude (-40%, +134% with, -65%, +314% without leukodepletion) after coincubation with autologous blood. In contrast, allogeneic erythrocytes had only little effects (TNF-alpha -6%, IL-10 +36%) even at this high transfusion equivalent. Conclusion These data suggest that banked whole blood has an immunosuppressive effect that is largely attributable to storage-dependent factors. These factors are partially removed by prestorage leukodepletion, while the contribution of alloantigens is of minor significance. Immunosuppressive effects are least apparent with leukodepleted erythrocytes, suggesting that the presence of plasma during storage is required for the immunosuppressive effect to develop.

scholarly journals Effects of Storage Conditions on Postharvest Qualities in Garlic Bulbs (Allium sativum L.)

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 805C-805
Author(s):  
Sun-Tay Choi ◽  
Ro-Na Bae* ◽  
Dae-Sung Chung ◽  
Seung-Koo Lee
Keyword(s):  
Weight Loss ◽  
Low Temperature ◽  
Pyruvic Acid ◽  
Room Temperature ◽  
Allium Sativum ◽  
Storage Period ◽  
Storage Conditions ◽  
Controlled Atmosphere ◽  
Atmosphere Storage ◽  
And Storage

To investigate quality changes of garlic associated with cultivars and storage conditions, northern type `Seosan' and sub-tropical type `Daeseo' garlics were stored at controlled atmosphere (O2 3%, CO2 5%, -1 ± 1°C) condition, low temperature (-1 ± 1°C), and room temperature (20 ± 5°C). The rate of sprouting, weight loss, enzymatic pyruvic acid content, and degree of greening in crushed garlic were determined during storage. The rate of sprouting was higher in `Daeseo' than in `Seosan' garlic in all storage conditions. Sprouting was effectively suppressed in low temperature and controlled atmosphere storage. Weight loss in `Daeseo' garlic was higher than in `Seosan' garlic. Enzymatic pyruvic acid (EP) contents increased for 3 months storage period, and then decreased gradually as the storage period was prolonged at room or low temperatures. However, EP content decreased dramatically during storage under CA condition in both cultivars. When garlic bulbs were crushed, greening appeared in the garlic stored at low temperature for more than one month. However, greening did not occur in the crushed garlic bulbs stored in CA condition.

scholarly journals DNA Extract was from filter paper stored at room temperature v1

2021 ◽  
Author(s):  
Katie Izenour ◽  
Anwar Kalalah ◽  
Fayez Salib ◽  
Sarah not provided Zohdy
Keyword(s):  
Filter Paper ◽  
Whole Blood ◽  
Room Temperature ◽  
Cold Chain ◽  
Western World ◽  
Blood Collection ◽  
Conventional Pcr ◽  
Liquid Form ◽  
And Storage ◽  
Babesia Spp

Preservation of samples requiring cold-chain storage is an often unavoidable challenge especially when doing laboratory work outside the western world. Samples are a precious commodity and it is imperative to maintain their viability. One method for collection and storage of samples in a liquid form (blood, saliva, serum) at room temperature is on filter paper cards like the Cellabs Tropbio Filter Paper Blood Collection Disks™. Methods and protocols exist for the use and recovery of whole blood from filter paper discs. This protocol describes the wash and recovery of DNA extracted from whole blood using the Qiagen DNeasy Extraction kit and dried on filter paper, re-suspended after several months of room temperature storage and use in conventional PCR. This protocol was used to suspend DNA extracted from the wholeblood of Dogs in Cairo, Egypt. The DNA was extracted using the Qiagen DNEasy Extraction Kit and placed on Cellabs Tropbio Filter Paper Blood Collection Disks™ (filter paper). The DNA dried on the filter paper overnight and was stored in plastic self-closure baggies for several months before being washed off and used in Conventional PCR. The use case described here is of a dog who was PCR positive for Babesia spp. using a sample of whole blood washed from the filter paper as well as dried DNA from filter paper.

Effects of Flavorings, Storage Conditions, and Storage Time on Survival of Staphylococcus aureus in Sürk Cheese

2005 ◽  
Vol 68 (7) ◽  
pp. 1487-1491 ◽  
Author(s):  
TUĞRUL M. MASATCIOĞLU ◽  
YAHYA K. AVŞAR
Keyword(s):  
Staphylococcus Aureus ◽  
Room Temperature ◽  
Storage Time ◽  
Storage Conditions ◽  
Black Pepper ◽  
Ash Content ◽  
Mold Growth ◽  
Storage Duration ◽  
And Storage ◽  
Over Time

The objectives of this study were to determine the cumulative effects of flavorings (chili pepper, thyme, mint, cumin, nutmeg, allspice, clove, cinnamon, black pepper, salt, and hot red pepper paste), storage conditions, and storage time on the survival of Staphylococcus aureus in Sürk cheese and to monitor the associated chemical changes. Sürk cheese, a traditional Turkish cheese, was produced by heating diluted nonfat yogurt and adding flavorings to the resultant acid-heat curd. The cheese was later inoculated with S. aureus, shaped conically, and stored aerobically for mold growth and anaerobically in olive oil for 30 days at room temperature. The moisture content of aerobically stored cheese decreased over time and led to increases in total solids, salt, salt-in-moisture, and ash content during ripening (P &lt; 0.05). The presence or absence of the flavorings had no significant effect, whereas storage conditions and storage duration decreased the survival of S. aureus (P &lt; 0.05).

scholarly journals The influence of geographical origin on honey composition studied by Polish and Slovak honeys

2019 ◽  
Vol 37 (No. 4) ◽  
pp. 232-238 ◽  
Author(s):  
Monika Tomczyk ◽  
Maria Tarapatskyy ◽  
Małgorzata Dżugan
Keyword(s):  
Geographical Origin ◽  
Total Phenolics ◽  
Storage Conditions ◽  
Botanical Origin ◽  
Colour Intensity ◽  
Statistical Tool ◽  
Sugar Profile ◽  
The Impact ◽  
And Storage

Honey composition is mainly affected by botanical origin, however geographical factors as well as beekeeping practice and storage conditions can also influence its quality. The aim of the study was to determine the impact of geographical origin on physicochemical quality and biological activity of honey. For this reason Polish and Slovak varietal honeys, including per each country: 10 multifloral, 5 tilia, 5 rape, 5 acacia and 5 forest were compared according to their physicochemical parameters (free acidity, pH, electrical conductivity, moisture content, and colour intensity), sugar profile, diastase activity, as well as antioxidant activity (DPPH and FRAP tests, as well as photochemiluminescence method). Moreover, total phenolics compounds and flavonoids content were determined. The most significant differences (P &lt; 0.05) between Polish and Slovak counterparts were found for tilia while the lowest for rape honeys. The impact of geographical origin on overall quality of honey was proved by PCA statistical tool.

Generation of Kinins during Preparation and Storage of Leukoreduced Platelet Concentrates.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 947-947
Author(s):  
Marie Eve Moreau ◽  
Louis Thibault ◽  
Anik Désormeaux ◽  
François Marceau ◽  
Marie-Joëlle de Grandmont ◽  
...  
Keyword(s):  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Storage System ◽  
Reference Population ◽  
Storage Conditions ◽  
Blood Collection ◽  
Converting Enzyme Inhibitors ◽  
Pharmacological Characterization ◽  
And Storage

Abstract BACKGROUND. Severe hypotensive reactions can occur following transfusion of blood components, particularly with platelets concentrates (PCs) leukoreduced with negatively- charged filters. Bradykinin (BK)-related peptides were proposed as a possible cause of this side-effect. This study evaluated the effects of leukoreduction and storage conditions on the levels of two kinins (BK and des-Arg9-BK) in PCs. METHODS. Whole blood donations (n=35) were processed using current PRP (platelet-rich plasma) procedure to prepare leukoreduced and unfiltered components by Leukotrap® RC-PL Whole Blood Collection, Filtration and Storage System. PCs and plasma were stored for 7 days at 20–24°C with agitation. Levels of BK and des-Arg9-BK were measured by specific enzyme immunoassays and HPLC at day 0, 2, 5 and 7 of storage. Mechanisms potentially responsible for accumulation of BK and des-Arg9-BK were studied. RESULTS. On day 0, kinins were measured in significantly higher concentrations in leukoreduced (BK: 101 ± 157 pg/mL; des-Arg9-BK: 194 ± 191 pg/mL) vs unfiltered PCs (BK: 71 ± 121 pg/mL; des-Arg9-BK: 98 ± 114 pg/mL). During storage, both kinins peaked on day 5, with concentrations higher than 1 ng/mL in 22% of leukoreduced as well as unfiltered PCs. Physicochemical and pharmacological characterization of immunoreactive kinins confirmed their nature. In vitro activation of the contact system of the corresponding platelet-poor plasma (PPP) showed that a high kinin concentration on day 5 of the storage corresponded to a low kinin-forming capacity of plasma. On day 7, BK was no longer elevated presumably due to its degradation and the depletion of kinin-forming capacity of the plasma in stored PCs. The activity of metallopeptidases that metabolize BK-related peptides in plasma from PCs were at levels similar to those recorded in the plasma of a normal reference population and were unaffected by storage. CONCLUSIONS. Our results indicate that filtration and storage conditions of PCs contribute to generation of pharmacologically relevant bradykinin levels that might pose a risk in susceptible patients. The clinical relevance of such high concentrations of kinins in PCs remains to be established but could potentially be significant especially in patients treated with angiotensin I-converting enzyme-inhibitors that affect the pathway of BK degradation.

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