scholarly journals Identification, Molecular Profiling, Docking Studies and determination of In-vivo Anti-Inflammatory Potential of Teucrium Stocksianum Bioss Fixed Oil (FO)

2021 ◽  
Author(s):  
Syed Muhammad Mukarram Shah ◽  
Syed Muhammad Hassan Shah ◽  
Rehman Zafar ◽  
Mohammad Ijaz Khan ◽  
Fida Hussain ◽  
...  
Keyword(s):  
Body Weight ◽  
Arachidonic Acid ◽  
Acute Toxicity ◽  
Chemical Constituents ◽  
Saturated Fatty Acids ◽  
Prostaglandin E ◽  
Paw Edema ◽  
Teucrium Stocksianum ◽  
Edema Model ◽  
Anti Inflammatory

Abstract Background: Considering the positive and valuable upshots of the naturally occurring ingredients as the complementary treatment for many ailments, this study investigates the Teucrium Stocksianum Bioss Fixed Oil (FO) for the treatment of inflammation.Methods: Different plants of the Teucrium genera have been employed occasionally for the management of different disorders. Teucrium stocksianum (Lamiaceae) is traditionally use as antipyretic along with remedy of inflammation, diabetes and tumors. It also occupies the capacity to purify the blood. The aim of this study is to determine the chemical constituents and anti-inflammatory potentials of Teucrium stocksianum fixed oil (FO). Qualitative and quantitative analysis of fixed oil were performed via GC-MS (Gas Chromatography coupled with Mass Spectrometer). Preliminary inflammation antagonistic activity was calculated in mice using the carrageenan-induced paw edema model while the mechanism behind this inflammatory antagonistic effect was determined by employing various inflammogens including arachidonic acid, prostaglandins E2 and leukotriene via paw edema model.Results: The acute toxicity of fixed oil was determined at 3, 6 and 10 ml/kg body weight of the mouse. The GC-MS analysis documented 21 various unsaturated and saturated fatty acids elements. The methyl esters of octadecadienoic and linoleic acids were found predominantly at 22.60% and 23.84% respectively. In preliminary screening, FO demonstrated substantial anti-inflammatory capacity (67.87%, **P ˂ 0.01) in carrageenan induced paw edema model at 3 ml/kg body weight. The activity reached a peak value at the 3rd hour and continued persistent until the 5th hour of sample administration. FO displayed dose-dependent inhibition against all inflammogens at 3 ml/kg at 3rd h of the FO administration. These have shown marvelous protection i.e. 64.92%, 68.75%and 58.3% against arachidonic acid, prostaglandin E2 and leukotriene accordingly, at 3 ml/kg. The arachidonic acid antagonist (Caffeic acid) demonstrated 69.43% activity at 100 mg/kg after 3rd h of its administration while in the acute toxicity test, FO has shown no grass lethality at 10 ml/kg dose.Conclusion: According to our findings, the FO of T. stocksianum exhibits inflammation antagonistic potential through both lipoxygenase (LOX) and cyclooxygenase (COX) enzyme inhibition strategies that intensely sustenance the traditional practice of T. stocksianum in the management of multiple pathological inflammatory situations. The computed binding energies of the molecules exposed have synergistic potential to avert inflammation.

scholarly journals Anti-Inflammatory and Antimicrobial Activities of Compounds Isolated from Distichochlamys benenica

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Ty Viet Pham ◽  
Hanh Nhu Thi Hoang ◽  
Hoai Thi Nguyen ◽  
Hien Minh Nguyen ◽  
Cong Thang Huynh ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Antimicrobial Activities ◽  
Coumaric Acid ◽  
Paw Edema ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Bioactive Properties ◽  
Edema Model ◽  
Anti Inflammatory ◽  
Available Information

Distichochlamys benenica is a native black ginger that grows in Vietnam. In point of fact, there is limitation of available information in the literature making mention of the chemical constituents and bioactive properties of this plant. This study is aimed at isolating trans-o-coumaric acid (1), trans-cinnamic acid (2), and borneol (3) from the rhizomes of D. benenica Q.B.Nguyen & Škorničk and evaluate the anti-inflammatory and antimicrobial activities of 1-3 using the carrageenan paw edema model and the dilution broth method, respectively. This revealed that 1 was as effective as diclofenac in reducing the intensity of the edema development. The in silico research showed that the activity of 1 might be derived from inhibiting COX-2 by generating h-bonds at the positions of Arg 120, Tyr 355, and Arg 513 residues. The antimicrobial activities against Gram-positive strains (Staphylococcus aureus and Bacillus subtilis) were comparable, with the minimum inhibitory concentrations ranging from 1.52 to 3.37 mM. This is the first study of the bioactivity of compounds isolated from D. benenica Q.B.Nguyen & Škorničk. Our results suggest that 1 may be a nature-derived compound which demonstrates the anti-inflammatory properties and inhibit the proliferation of several Gram-positive bacteria.

scholarly journals SYNTHESIS AND PRELIMINARY PHARMACOLOGICAL EVALUATION OF NEW NAPROXEN ANALOGUES HAVING 1, 2, 4-TRIAZOLE-3-THIOL

2017 ◽  
Vol 9 (7) ◽  
pp. 66
Author(s):  
Monther F. Mahdi ◽  
Noor H. Naser ◽  
Nethal H. Hammud
Keyword(s):  
Aromatic Aldehydes ◽  
Heterocyclic Ring ◽  
Inflammatory Activity ◽  
Control Group ◽  
Paw Edema ◽  
Dose Equivalent ◽  
Edema Model ◽  
Anti Inflammatory ◽  
Derivatives Of

Objective: The objective of this search was to synthesize a new naproxen analogues having a 1,2,4-triazole-3-thiol heterocyclic ring, and preliminary pharmacological assessment of the anti-inflammatory activity of the synthesized compounds. Methods: The synthesis of naproxen analogues that having 1,2,4-triazole-3-thiol heterocyclic ring occur through esterification of naproxen, and then its reaction with hydrazine hydrate, and carbon disulfide, finally different aromatic aldehydes reacted with triazole derivatives of naproxen containing amino group to produce schiff bases.Results: In vivo acute anti-inflammatory activity of the synthesize compounds (Va-Vd) was evaluated in rats using egg-white induced edema model of inflammation in a dose equivalent to (50 mg/kg) of naproxen. All tested compounds were produced a significant reduction in paw edema with respect to the effect of propylene glycol 50% v/v (control group). Compound Vd produced superior anti-inflammatory activity compared to naproxen.Conclusion: The results obtained in this work give evidence about the valid synthesis of 1,2,4 triazole-3-thiol derivatives of naproxen, which reacted with different aldehydes to yield several schiff bases. The incorporation of benzaldehyde possess para-electron donating group (para-hydroxyl benzaldehyde) will increase the anti-inflammatory activity of naproxen.

scholarly journals Retraction Note: Release-Active Dilutions of Diclofenac Enhance Anti-inflammatory Effect of Diclofenac in Carrageenan-Induced Rat Paw Edema Model

Inflammation ◽  
2020 ◽  
Author(s):  
Sachin S. Sakat ◽  
Kamaraj Mani ◽  
Yulia O. Demidchenko ◽  
Evgeniy A. Gorbunov ◽  
Sergey A. Tarasov ◽  
...  
Keyword(s):  
Paw Edema ◽  
Rat Paw Edema ◽  
Edema Model ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Inflammatory Effect

scholarly journals Antioxidant and Anti-Inflammatory Effects of Genus Gynura: A Systematic Review

2020 ◽  
Vol 11 ◽  
Author(s):  
Jiah Ning Tan ◽  
Shamin Mohd Saffian ◽  
Fhataheya Buang ◽  
Zakiah Jubri ◽  
Ibrahim Jantan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Systematic Review ◽  
Clinical Studies ◽  
Glycogen Synthase ◽  
Chemical Constituents ◽  
Future Research ◽  
Prostaglandin E ◽  
Pharmacokinetic Studies ◽  
Related Factor ◽  
Anti Inflammatory

Background:Gynura species have been used traditionally to treat various ailments, such as fever, pain, and to control blood glucose level. This systematic review critically discusses studies regarding Gynura species that exhibited antioxidant and anti-inflammatory effects, thus providing perspectives and instructions for future research of the plants as a potential source of new dietary supplements or medicinal agents.Methods: A literature search from internet databases of PubMed, Scopus, Science Direct, e-theses Online Service, and ProQuest was carried out using a combination of keywords such as “Gynura,” “antioxidant,” “anti-inflammatory,” or other related words. Research articles were included in this study if they were experimental (in vitro and in vivo) or clinical studies on the antioxidant or anti-inflammatory effects of Gynura species and if they were articles published in English.Results: Altogether, 27 studies on antioxidant and anti-inflammatory effects of Gynura species were selected. The antioxidant effects of Gynura species were manifested by inhibition of reactive oxygen species production and lipid peroxidation, modulation of glutathione-related parameters, and enzymatic antioxidant production or activities. The anti-inflammatory effects of Gynura species were through the modulation of inflammatory cytokine production, inhibition of prostaglandin E2 and nitric oxide production, cellular inflammatory-related parameters, and inflammation in animal models. The potential anti-inflammatory signaling pathways modulated by Gynura species are glycogen synthase kinase-3, nuclear factor erythroid 2-related factor 2, PPARγ, MAPK, NF-κB, and PI3K/Akt. However, most reports on antioxidant and anti-inflammatory effects of the plants were on crude extracts, and the chemical constituents contributing to bioactivities were not clearly understood. There is a variation in quality of studies in terms of design, conduct, and interpretation, and in-depth studies on the underlying mechanisms involved in antioxidant and anti-inflammatory effects of the plants are in demand. Moreover, there is limited clinical study on antioxidant and anti-inflammatory effects of Gynura species.Conclusion: This review highlighted antioxidant and anti-inflammatory effects of genus Gynura and supported their traditional uses to treat oxidative stress and inflammatory-related diseases. This review is expected to catalyze further studies on genus Gynura. However, extensive preclinical data need to be generated from toxicity and pharmacokinetic studies before clinical studies can be pursued for their development into clinical medicines to treat oxidative stress and inflammatory conditions.

scholarly journals PHYTOCHEMICAL SCREENING AND EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF AERIAL PART EXTRACTS OF PLANTAGO MAJOR L.

2017 ◽  
Vol 10 (9) ◽  
pp. 307
Author(s):  
Jyoti Vandana ◽  
Gupta A K ◽  
Alok Mukerjee
Keyword(s):  
Aerial Part ◽  
Iridoid Glycosides ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Plantago Major ◽  
Phytochemical Screening ◽  
Paw Edema ◽  
Edema Model ◽  
Anti Inflammatory ◽  
Wistar Albino Rats

  Objective: This study aims to perform phytochemical screening and investigate anti-inflammatory activity of extract from aerial part of the plant Plantago major L.Methods: Preliminary phytochemical screening of plant extract was carried out using standard qualitative procedures and anti-inflammatory activity was evaluated using carrageenan-induced paw edema model in male Wistar albino rats using plethysmometer. Diclofenac sodium (10 mg/kg, b.w.) was used as standard drug.Results: Preliminary phytochemical screening of the different extracts revealed that they were rich in secondary metabolite compounds such as carbohydrates, tannins, steroids, flavonoids, terpenoids, glycosides, fats and oils, alkaloids, phenolic compounds, and iridoid glycosides. The anti-inflammatory activity was evaluated using carrageenan-induced paw edema models in Wistar albino rats. The anti-inflammatory activity of methanolic extract (ME) was found to be dose dependent in carrageenan-induced paw edema model. The ME has shown significant (p<0.05) inhibition of paw edema, 61.11% and 55.55% on 4th hr at the doses of 900 and 600 mg/kg, respectively.Conclusion: The results of the present study demonstrate that ME of the aerial parts of the plant possess significant (p<0.05) anti-inflammatory potential.

scholarly journals Inhibitory Effects of Traditional Herbal Formula Pyungwi-San on Inflammatory ResponseIn VitroandIn Vivo

2013 ◽  
Vol 2013 ◽  
pp. 1-19 ◽  
Author(s):  
Ji Young Cha ◽  
Ji Yun Jung ◽  
Jae Yup Jung ◽  
Jong Rok Lee ◽  
Il Je Cho ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Inflammatory Mediators ◽  
Raw 264.7 Cells ◽  
Prostaglandin E ◽  
Herbal Formula ◽  
Paw Edema ◽  
Cox 2 ◽  
Anti Inflammatory

Pyungwi-san (PWS) is a traditional basic herbal formula. We investigated the effects of PWS on induction of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), pro-inflammatory cytokines (interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α)) and nuclear factor-kappa B (NF-κB) as well as mitogen-activated protein kinases (MAPKs) in lipopolysaccharide-(LPS-) induced Raw 264.7 cells and on paw edema in rats. Treatment with PWS (0.5, 0.75, and 1 mg/mL) resulted in inhibited levels of expression of LPS-induced COX-2, iNOS, NF-κB, and MAPKs as well as production of prostaglandin E2(PGE2), nitric oxide (NO), IL-6, and TNF-αinduced by LPS. Our results demonstrate that PWS possesses anti-inflammatory activities via decreasing production of pro-inflammatory mediators through suppression of the signaling pathways of NF-κB and MAPKs in LPS-induced macrophage cells. More importantly, results of the carrageenan-(CA-) induced paw edema demonstrate an anti-edema effect of PWS. In addition, it is considered that PWS also inhibits the acute edematous inflammations through suppression of mast cell degranulations and inflammatory mediators, including COX-2, iNOS and TNF-α. Thus, our findings may provide scientific evidence to explain the anti-inflammatory properties of PWSin vitroandin vivo.

Lipopolysaccharide-induced paw edema model for detection of cytokine modulating anti-inflammatory agents

2004 ◽  
Vol 4 (7) ◽  
pp. 901-909 ◽  
Author(s):  
Bhargavi N.L Vajja ◽  
Suresh Juluri ◽  
Manju Kumari ◽  
Labonyamoy Kole ◽  
Ranjan Chakrabarti ◽  
...  
Keyword(s):  
Paw Edema ◽  
Edema Model ◽  
Anti Inflammatory
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