scholarly journals Parecoxib alleviates the inflammatory effect of leukocyte-rich platelet-rich plasma in normal rabbit tendons

2020 ◽  
Author(s):  
Ming Zhou ◽  
Ning Wang ◽  
Gang Wang ◽  
Zishan Jia ◽  
Xiaolei Qi
Keyword(s):  
Inflammatory Response ◽  
Patellar Tendon ◽  
Platelet Rich Plasma ◽  
White Blood Cells ◽  
Nsaid Group ◽  
Significant Difference ◽  
Cell Components ◽  
Hematoxylin And Eosin ◽  
Inflammatory Drugs ◽  
Group 2

Abstract Background: Platelet-rich plasma (PRP) is widely used to treat tendon injuries. Its therapeutic effect varies depending on the different cell components, and white blood cells (WBCs) may play an important role in this phenomenon. The purpose of this study was to evaluate how PRP with different concentrations of WBCs affect normal rabbit tendon and assess whether non-steroidal anti-inflammatory drugs (NSAIDs) can suppress the catabolic effects of WBCs.Methods: Sixteen adult New Zealand White rabbits were used. Blood samples were collected from each rabbit, and PRP was extracted following two different protocols to obtain leukocyte-poor PRP (LP-PRP) and leukocyte-rich PRP (LR-PRP). LP-PRP or LR-PRP was injected into the patellar tendon of each rabbit, while normal saline (NS) was injected as control. In LR-PRP + NSAID group, Parecoxib was administered after LR-PRP injection. For each group, 2 rabbits were euthanatized at day 5 and 14. The patellar tendons were collected and stained with hematoxylin and eosin. A semi-quantitative approach was used to assess the inflammatory response and tendon destruction based on the evaluation of the WBCs, vascularization, fiber structure, and fibrosis.Results: The LR-PRP group exhibited a higher total tendon score than the LP-PRP group at day 5 after PRP injection, but there was no significant difference between the two groups at day 14. For the NSAID group, the tendon score was lower than that of the LR-PRP group both at day 5 and 14.Conclusion: LR-PRP can promote a higher inflammatory response than LP-PRP in the normal rabbit patellar tendon, and this effect can be suppressed by NSAIDs.

scholarly journals Parecoxib alleviates the inflammatory effect of leukocyte-rich platelet-rich plasma in normal rabbit tendons

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Ming Zhou ◽  
Ning Wang ◽  
Gang Wang ◽  
Zishan Jia ◽  
Xiaolei Qi
Keyword(s):  
Inflammatory Response ◽  
Patellar Tendon ◽  
Platelet Rich Plasma ◽  
White Blood Cells ◽  
Nsaid Group ◽  
Significant Difference ◽  
Cell Components ◽  
Hematoxylin And Eosin ◽  
Inflammatory Drugs ◽  
Group 2

Abstract Background Platelet-rich plasma (PRP) is widely used to treat tendon injuries. Its therapeutic effect varies depending on the different cell components, and white blood cells (WBCs) may play an important role in this phenomenon. The purpose of this study was to evaluate how PRP with different concentrations of WBCs affect normal rabbit tendon and assess whether non-steroidal anti-inflammatory drugs (NSAIDs) can suppress the catabolic effects of WBCs. Methods Sixteen adult New Zealand White rabbits were used. Blood samples were collected from each rabbit, and PRP was extracted following two different protocols to obtain leukocyte-poor PRP (LP-PRP) and leukocyte-rich PRP (LR-PRP). LP-PRP or LR-PRP was injected into the patellar tendon of each rabbit, while normal saline (NS) was injected as control. In LR-PRP + NSAID group, Parecoxib was administered after LR-PRP injection. For each group, 2 rabbits were euthanatized at day 5 and 14. The patellar tendons were collected and stained with hematoxylin and eosin. A semi-quantitative approach was used to assess the inflammatory response and tendon destruction based on the evaluation of the WBCs, vascularization, fiber structure, and fibrosis. Results The LR-PRP group exhibited a higher total tendon score than the LP-PRP group at day 5 after PRP injection, but there was no significant difference between the two groups at day 14. For the NSAID group, the tendon score was lower than that of the LR-PRP group both at day 5 and 14. Conclusion LR-PRP can promote a higher inflammatory response than LP-PRP in the normal rabbit patellar tendon, and this effect can be suppressed by NSAIDs.

scholarly journals Parecoxib alleviates the inflammatory effect of leukocyte-rich platelet-rich plasma in normal rabbit tendons

2020 ◽  
Author(s):  
Ming Zhou ◽  
Ning Wang ◽  
Gang Wang ◽  
Zishan Jia ◽  
Xiaolei Qi
Keyword(s):  
Inflammatory Response ◽  
Patellar Tendon ◽  
Platelet Rich Plasma ◽  
White Blood Cells ◽  
Nsaid Group ◽  
Significant Difference ◽  
Cell Components ◽  
Hematoxylin And Eosin ◽  
Inflammatory Drugs ◽  
Group 2

Abstract Background: Platelet-rich plasma (PRP) is widely used to treat tendon injuries. Its therapeutic effect varies depending on the different cell components, and white blood cells (WBCs) may play an important role in this phenomenon. The purpose of this study was to evaluate how PRP with different concentrations of WBCs affect normal rabbit tendon and assess whether non-steroidal anti-inflammatory drugs (NSAIDs) can suppress the catabolic effects of WBCs. Methods: Sixteen adult New Zealand White rabbits were used. Blood samples were collected from each rabbit, and PRP was extracted following two different protocols to obtain leukocyte-poor PRP (LP-PRP) and leukocyte-rich PRP (LR-PRP). LP-PRP or LR-PRP was injected into the patellar tendon of each rabbit, while normal saline (NS) was injected as control. In LR-PRP + NSAID group, Parecoxib was administered after LR-PRP injection. For each group, 2 rabbits were euthanatized at day 5 and 14. The patellar tendons were collected and stained with hematoxylin and eosin. A semi-quantitative approach was used to assess the inflammatory response and tendon destruction based on the evaluation of the WBCs, vascularization, fiber structure, and fibrosis. Results: The LR-PRP group exhibited a higher total tendon score than the LP-PRP group at day 5 after PRP injection, but there was no significant difference between the two groups at day 14. For the NSAID group, the tendon score was lower than that of the LR-PRP group both at day 5 and 14. Conclusion: LR-PRP can promote a higher inflammatory response than LP-PRP in the normal rabbit patellar tendon, and this effect can be suppressed by NSAIDs.

scholarly journals Parecoxib alleviates the inflammatory effect of leukocyte-rich platelet-rich plasma in normal rabbit tendons

2020 ◽  
Author(s):  
Ming Zhou ◽  
Ning Wang ◽  
Gang Wang ◽  
Zishan Jia ◽  
Xiaolei Qi
Keyword(s):  
Inflammatory Response ◽  
Patellar Tendon ◽  
Platelet Rich Plasma ◽  
White Blood Cells ◽  
Nsaid Group ◽  
Significant Difference ◽  
Cell Components ◽  
Hematoxylin And Eosin ◽  
Inflammatory Drugs ◽  
Group 2

Abstract Background: Platelet-rich plasma (PRP) is widely used to treat tendon injuries. Its therapeutic effect varies depending on the different cell components, and white blood cells (WBCs) may play an important role in this phenomenon. The purpose of this study was to evaluate how PRP with different concentrations of WBCs affect normal rabbit tendon and assess whether non-steroidal anti-inflammatory drugs (NSAIDs) can suppress the catabolic effects of WBCs.Methods: Sixteen adult New Zealand White rabbits were used. Blood samples were collected from each rabbit, and PRP was extracted following two different protocols to obtain leukocyte-poor PRP (LP-PRP) and leukocyte-rich PRP (LR-PRP). LP-PRP or LR-PRP was injected into the patellar tendon of each rabbit, while normal saline (NS) was injected as control. In LR-PRP + NSAID group, Parecoxib was administered after LR-PRP injection. For each group, 2 rabbits were euthanatized at day 5 and 14. The patellar tendons were collected and stained with hematoxylin and eosin. A semi-quantitative approach was used to assess the inflammatory response and tendon destruction based on the evaluation of the WBCs, vascularization, fiber structure, and fibrosis.Results: The LR-PRP group exhibited a higher total tendon score than the LP-PRP group at day 5 after PRP injection, but there was no significant difference between the two groups at day 14. For the NSAID group, the tendon score was lower than that of the LR-PRP group both at day 5 and 14.Conclusion: LR-PRP can promote a higher inflammatory response than LP-PRP in the normal rabbit patellar tendon, and this effect can be suppressed by NSAIDs.

scholarly journals Effect of Oral Losartan on Orthobiologics: Implications for Platelet-Rich Plasma and Bone Marrow Concentrate—A Rabbit Study

2020 ◽  
Vol 21 (19) ◽  
pp. 7374
Author(s):  
Gilberto Y. Nakama ◽  
Sabrina Gonzalez ◽  
Polina Matre ◽  
Xiaodong Mu ◽  
Kaitlyn E. Whitney ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteochondral Defect ◽  
Platelet Rich Plasma ◽  
Control Group ◽  
Bone Marrow Concentrate ◽  
Significant Difference ◽  
Losartan Group ◽  
Group 2 ◽  
And Control ◽  
Tgf Β1

Recent efforts have focused on customizing orthobiologics, such as platelet-rich plasma (PRP) and bone marrow concentrate (BMC), to improve tissue repair. We hypothesized that oral losartan (a TGF-β1 blocker with anti-fibrotic properties) could decrease TGF-β1 levels in leukocyte-poor PRP (LP-PRP) and fibrocytes in BMC. Ten rabbits were randomized into two groups (N = 5/group): osteochondral defect + microfracture (control, group 1) and osteochondral defect + microfracture + losartan (losartan, group 2). For group 2, a dose of 10mg/kg/day of losartan was administrated orally for 12 weeks post-operatively. After 12 weeks, whole blood (WB) and bone marrow aspirate (BMA) samples were collected to process LP-PRP and BMC. TGF-β1 concentrations were measured in WB and LP-PRP with multiplex immunoassay. BMC cell populations were analyzed by flow cytometry with CD31, CD44, CD45, CD34, CD146 and CD90 antibodies. There was no significant difference in TGF-β1 levels between the losartan and control group in WB or LP-PRP. In BMC, the percentage of CD31+ cells (endothelial cells) in the losartan group was significantly higher than the control group (p = 0.008), while the percentage of CD45+ cells (hematopoietic cells-fibrocytes) in the losartan group was significantly lower than the control group (p = 0.03).

scholarly journals Effect of Platelet-Rich Plasma (PRP) versus Autologous Whole Blood on Pain and Function Improvement in Tennis Elbow: A Randomized Clinical Trial

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Seyed Ahmad Raeissadat ◽  
Leyla Sedighipour ◽  
Seyed Mansoor Rayegani ◽  
Mohammad Hasan Bahrami ◽  
Masume Bayat ◽  
...  
Keyword(s):  
Whole Blood ◽  
Tennis Elbow ◽  
Platelet Rich Plasma ◽  
Autologous Blood ◽  
Pressure Pain Threshold ◽  
Lateral Epicondylitis ◽  
Autologous Whole Blood ◽  
Significant Difference ◽  
Group 2 ◽  
And Function

Background. Autologous whole blood and platelet-rich plasma (PRP) have been both suggested to treat chronic tennis elbow. The aim of the present study was to compare the effects of PRP versus autologous whole blood local injection in chronic tennis elbow. Methods. Forty patients with tennis elbow were randomly divided into 2 groups. Group 1 was treated with a single injection of 2 mL of autologous PRP and group 2 with 2 mL of autologous blood. Tennis elbow strap, stretching, and strengthening exercises were administered for both groups during a 2-month followup. Pain and functional improvements were assessed using visual analog scale (VAS), modified Mayo Clinic performance index for the elbow, and pressure pain threshold (PPT) at 0, 4, and 8 weeks. Results. All pain and functional variables including VAS, PPT, and Mayo scores improved significantly in both groups 4 weeks after injection. No statistically significant difference was noted between groups regarding pain scores in 4-week follow-up examination (P>0.05). At 8-week reevaluations, VAS and Mayo scores improved only in PRP group (P<0.05). Conclusion. PRP and autologous whole blood injections are both effective to treat chronic lateral epicondylitis. PRP might be slightly superior in 8-week followup. However, further studies are suggested to get definite conclusion.

scholarly journals FEATURES OF HEMATOLOGICAL INDICES IN PATIENTS WITH IMPAIRED CARDIAC FUNCTION IN UP-TO-DATE CLINICAL PRACTICE

2021 ◽  
Vol 19 (4) ◽  
Author(s):  
V.K. Tashchuk ◽  
R.A. Nesterovska ◽  
V.O. Kalarash
Keyword(s):  
New York ◽  
White Blood Cells ◽  
Heart Association ◽  
Functional Class ◽  
Hematological Indices ◽  
Neutrophil Lymphocyte Ratio ◽  
Versus Group ◽  
Significant Difference ◽  
Group 2 ◽  
Group 1

Purpose – to investigate the distribution of hematological indices in patients withcardiac insufficiency.Material and methods. Data of 26 case histories with diagnosis of IHD have beenanalyzed. Patients with stable angina pectoris of II-III functional class (FC), Diffusecardiosclerosis, complicated in 17 patients with syndromic manifestations of HF II-IIIFC according to New York Heart Association (NYHA), made up group 1, and 9 patientswithout CH -group 2. Of the group 1 surveyed, there were 8 men and 9 women. Ratioindex of leucocytes and erythrocyte sedimentation rate (ESR): L/ESR=L×ESR/100;Neutrophil-lymphocyte ratio index (N/Li); Lymphocyte to monocyte ratio index (Li / Mo);Lymphocyte to eosinophil index (Li/ E) was used among hematological markers.Results. Analyzing the haemogram data, it was found that group 1 patients, unlike group2 patients, had a significantly higher overall white blood cell count (7,96±1,73) × 109and (4,22 ± 0,24) × 109; p<0,05 due to the number of neutrophils (69,41 ± 6,21) % ascompared to group 2 (51,78±1,79)%; p<0,05 as well as a lower level of lymphocyteswas defined in group 1 (22,06±4,07)% versus group 2 (38,55±1,01)%; p<0,05. In genderstudies, men show elevated levels of eosinophils (4,12±0,83)%, as opposed to women(1,56±0,73) %; p<0,05 and lower level of lymphocytes (18,38±1,69)% versus (25,33±2,24)%; p<0,05 and women had a high level of total white blood cells (9,36±0,66) ×109 thanmen (6,36±0,99)×109; p<0,05, in particular lymphocytes. Analysis of hematologicalindices showed that there was a statistically significant difference in determining theN / Li index, which was increased in group 1 patients (3,28±0,78) у.о versus group 2(1,34±0,05) у.о, p<0,05.Conclusion. In gender comparisons, there is an increase in lymphocytes among womencompared to men, so we can assume that women are less susceptible to systemicinflammation. The increase in the N/Li index ratio is due to severe cardiovascularconsequences among patients with coronary heart disease complicated by heart failure.

scholarly journals Comparative Analysis of Biofilm Removal Efficacy by Multisonic Ultracleaning System and Passive Ultrasonic Activation

Materials ◽  
2019 ◽  
Vol 12 (21) ◽  
pp. 3492 ◽  
Author(s):  
Hae Won Choi ◽  
Seong Yeon Park ◽  
Mo Kwan Kang ◽  
Won Jun Shon
Keyword(s):  
Negative Control ◽  
Ultrasonic Activation ◽  
Treatment Groups ◽  
Mandibular Molars ◽  
Maxillary Molars ◽  
Biofilm Removal ◽  
Significant Difference ◽  
Hematoxylin And Eosin ◽  
Group 3 ◽  
Group 2

The purpose of this study was to compare disinfection and the biofilm removal efficacy of the GentleWave System (Sonendo, Inc., Laguna Hills, CA, USA) with passive ultrasonic activation method. Forty-seven freshly extracted human molars were inoculated with Enterococcus faecalis and cultured for five weeks to establish biofilm. Eight molars were tested for confirmation of infection. Four of the eight teeth were not inoculated in order to provide a negative control. The remaining 39 inoculated molars were randomly separated into three treatment groups (n = 13 per group): Group 1—no treatment, Group 2—conventional rotary instrumentation and passive ultrasonic activation, and Group 3—minimal instrumentation and the GentleWave System treatment. Roots were subsequently prepared per standard histological tissue processing procedures. Modified Brown and Brenn stained sections and Hematoxylin and Eosin stained sections were visualized at 4× and 13.5× magnification using a stereomicroscope. The sections were scored and blindly analyzed by two independent evaluators, including a histopathologist, to evaluate the presence of biofilm on canal wall. A significant difference was found between Group 2 and Group 3 in both apical and middle regions (p = 0.001) of the mesial roots of mandibular molars and mesiobuccal roots of maxillary molars. Group 3 revealed significantly less biofilm than the controls (p = 0.003). The GentleWave System demonstrated significantly greater reduction in biofilm within the mesial roots of mandibular molars and mesiobuccal roots of maxillary molars than those treated with conventional rotary instrumentation and passive ultrasonic activation protocol.

scholarly journals Orthosiphon versus Placebo in Nephrolithiasis with Multiple Chronic Complaints: A Randomized Control Trial

2009 ◽  
Vol 6 (4) ◽  
pp. 495-501 ◽  
Author(s):  
Amorn Premgamone ◽  
Pote Sriboonlue ◽  
Srinoi Maskasem ◽  
Wattana Ditsataporncharoen ◽  
Bungornsri Jindawong
Keyword(s):  
Myofascial Pain ◽  
White Blood Cells ◽  
Analog Scale ◽  
Significant Difference ◽  
Before And After ◽  
The Mean ◽  
Randomized Control ◽  
Group 2 ◽  
Primary Measure ◽  
Group 1

Nephrolithiasis in the communities of Northeast Thailand frequently presents with multiple chronic health complaints, i.e. myofascial pain, back pain, dyspepsia, arthralgia, headache, fatigue, frank paresthesia, dysuria and any of these aggravated by purine-rich food (PRF). We assessed the efficacy of Orthosiphon in treating subjects with at least two active symptoms and negative for urine white blood cells. Subjects were randomly allocated to two groups. Crude extract of Orthosiphon given in a capsule (equivalent to 1.6–1.8 g of dried leaves of Orthosiphon) two times a day to Group 1 (n= 36) and a placebo to Group 2 (n= 40) for 14 days. The medication for each subject was packed and its code kept secret until the data analysis. Both groups were asked not to consume any of 25 purine-rich foods (PRFs) during treatment. The primary measure was the reduced sum of active severity symptoms as recorded using the visual analog scale before and after therapy (i.e. on day 7 and 14). The data on 76 subjects were processed. The mean of the total scores (95% CI) of the symptoms in each group were decreased significantly (P< 0.001); 185.6 (153.3, 218.0) to 94.7 (58.2, 131.2) in the Orthosiphon group and 196.1 (164.4, 227.8) to 89.6 (62.8, 116.5) in the placebo group. When comparing between groups, no statistically significant difference was found. The mean consumption in PRFs was significantly decreased (P< 0.001) in both groups; however, Orthosiphon did not have additional benefit over placebo at 7 and 14 days of treatment during which they reduced these foods.

scholarly journals Biocompatibility of acetazolamide pastes in the subcutaneous tissue of rats

2009 ◽  
Vol 20 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Graziela Garrido Mori ◽  
Ivaldo Gomes de Moraes ◽  
Daniele Clapes Nunes ◽  
Lithiene Ribeiro Castilho ◽  
Wilson Roberto Poi
Keyword(s):  
Inflammatory Response ◽  
Light Microscopy ◽  
Propylene Glycol ◽  
Inflammatory Process ◽  
Subcutaneous Tissue ◽  
Similar Concentration ◽  
Gutta Percha ◽  
Significant Difference ◽  
Hematoxylin And Eosin ◽  
Main Component

This aim of this study was to investigate the biocompatibility of two experimental acetazolamide (AZ)-based pastes in the subcutaneous tissue of rats. Both pastes contained AZ as the main component in similar concentration. The vehicle in experimental paste 1 was saline, while experimental paste 2 was prepared with propylene glycol. Sixty polyethylene tubes were sealed at one end with gutta-percha (GP), which served as a control. Half of the tubes were filled with paste 1 and half with paste 2. The tubes were implanted in the subcutaneous tissue of 15 rats, being 4 tubes for each animal. The animals were killed 7, 15 and 45 days after surgery and the specimens were processed in laboratory. The histological sections were stained with hematoxylin and eosin and were analyzed by light microscopy. Scores were assigned to level of inflammatory process: 1- none; 2- mild; 3- moderate; 4- severe. The data were analyzed statistically by the Kruskal-Wallis test (p≤0.05). Paste 1 produced an inflammatory process at 7 days. However, the intensity of this inflammation decreased with time and was nearly absent at 45 days. No statistically significant difference (p>0.05) was observed between the control (GP) and paste 1. However, paste 2 produced inflammatory response at all study periods and differed significantly (p<0.05) from the control. In conclusion, in the present study, the experimental AZ-based paste 1 was considered as biocompatible as the control matrial (GP), while experimental paste 2 was irritating to rat subcutaneous tissue.

A Critical Investigation into the Existence of Circulating Platelet Aggregates

1986 ◽  
Vol 56 (01) ◽  
pp. 045-049 ◽  
Author(s):  
A R Saniabadi ◽  
G D O Lowe ◽  
R Madhok ◽  
K Spowart ◽  
B Shaw ◽  
...  
Keyword(s):  
Whole Blood ◽  
Ex Vivo ◽  
Platelet Rich Plasma ◽  
Venous Blood ◽  
White Blood Cells ◽  
Blood Platelet ◽  
Blood Collection ◽  
Significant Difference ◽  
Platelet Aggregates

SummaryBy a method of counting single platelets in diluted whole blood, platelet aggregates were quantified ex-vivo. Four groups: 20 thrombotic patients, 10 non-thrombotic patients, 10 healthy old controls and 10 healthy young controls were included in the study. Using a 19 gauge needle, with and without tubing, venous blood was taken into buffered EDTA, as a disaggregating agent and buffered EDTA-formalin, as the fixative. The amount of platelet aggregates quantified was affected by the quality of venepuncture or the rate of blood flow through the needle, but was unaffected by the presence of the tubing. There was no statistically significant difference between the four groups, in terms of the platelet aggregates quantified, but scanning electron microscopy revealed the presence of irreversible aggregates, composed of platelet red and white blood cells, in the blood of a greater number of thrombotic patients than non-thrombotic or healthy controls. Platelet aggregates were also quantified in aliquots of platelet rich plasma, and were found to be significantly greater than the corresponding values in whole blood. The difference appeared to be due to increased viscosity of the plasma, induced by the fixative which reduces platelet mobility during centrifugation. It is concluded that the platelet aggregates which disaggregate in bufffered EDTA may represent an artifact of blood collection; the irreversible aggregates are suspected to represent the in vivo circulating aggregates.

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