A Method for Assessing Axial and Temporal Effects of the Leaf Sheath on the Flexural Stiffness of the Maize Stem
Abstract Background: The leaf sheath of many plants has been observed to influence both stiffness of the stem and ultimate strength. The leaf sheath has been implicated in studies of maize “greensnap” (or “brittle-snap”) failure. However, but the influence of the sheath is still not well understood and few methods exist for studying the influence of the sheath. The goal of this study was to develop a method for assessing longitudinal and temporal patterns of sheath influence on flexural stiffness. This metric of flexural stiffness was chosen because it is non-destructive and has been shown to be highly predictive of bending strength. Results: A three-point bending test method was successfully developed for assessing the influence of the sheath on flexural stiffness. The method relies upon comparisons between pairs of tests at the same location (sheath present vs. absent). The influence of the sheath was statistically significant in all varieties tested. The test method provided insights into the longitudinal and spatial variation of sheath influence: sheath influence appears to be closely related to maturity since both spatial and temporal patterns of influence mirror the sigmoidal maturation patterns previously observed in maize stalks. Conclusions: The paired nature of this test method increases statistical significance while the non-destructive feature of this test allows for multiple tests along the length of the stalk. This method can be used to provide new insightsregarding how the leaf sheath influences stalk flexibility (and therefore strength). Preliminary results indicate that the influence of the sheath changes over the life span of the plant in parallel with maturation patterns. However, further studies will be needed to confirm this hypothesis more broadly and to study additional issues such as heritability and the influce of genotype and environment on sheath influence.