Expression profiling of LncRNA and miRNA in SDF-1-induced articular chondrocyte degeneration
Abstract Background With the in-depth exploration of the gene regulation network associated with the pathogenesis of osteoarthritis (OA), lncRNA has been found to play a major role in regulating the development of osteoarthritis. In this study, the expressions of miRNAs and lncRNAs in chondrocytes (2 days) of SDF-1-induced articular chondrocyte degeneration model and in normal chondrocytes were detected and the difference between them was visualized. The bioinformatics analysis was performed in parallel to elucidate the interactions between miRNAs and protein molecules. Results It was found that 186 lncRNA changes had significant statistical differences, of which 88 lncRNA were up-regulated and 98 lncRNA were down-regulated. A total of 684 miRNA had significant statistical differences in their expression changes. Gene Ontology and Kyoto Encyclopedia of Genes were performed for the gene set enrichment analysis to determine the key biological processes and pathways. The protein-protein interaction (PPI) network indicated that CXCL10, ISG15, MYC, MX1, OASL, FIICT1, RSAD2, MX2, IFI44, and LBST2 are the ten core genes. The PPI network identified the most important functional modules to elucidate the differential expression of miRNA. Conclusions These data may provide new insights into the molecular mechanisms of osteoarthritis chondrocyte degeneration, and the identification of lncRNA and miRNA can provide potential therapeutic targets for the diagnosis and differential diagnosis of osteoarthritis.