scholarly journals Effects of resistance exercise on lipolysis pathway in obese pre and postmenopausal women

2020 ◽  
Author(s):  
Kyu-Min Park ◽  
Seung-Taek Lim ◽  
Kun-Young Sung ◽  
Sunghwun Kang
Keyword(s):  
Resistance Exercise ◽  
Hormone Sensitive Lipase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fatty Tissue ◽  
Serum Samples ◽  
Protein Levels ◽  
Menopausal Women ◽  
Subcutaneous Fatty Tissue ◽  
Hormone Sensitive ◽  
Post Menopausal

Abstract Background and objectives: The purpose of study was to examine the effects of regular resistance exercise for 12 weeks on lipolysis pathway in pre- and post- menopausal women with obesity. Methods: Twenty-three pre- and post- menopausal women with body fat percentages of 30% or more divided into pre- menopausal group (n=9) and post- menopausal group (n=14). All subjects participated in resistance exercise training for 12 weeks. Anthropometric and physical fitness tests were performed on all participants. Protein analyses were performed with subcutaneous fatty tissue extracted, and the samples were analyzed of relevant protein levels changes by using Western blotting. All serum samples were submitted for enzyme-linked immunosorbent assay measurements of adipocyte factors. Results: After 12 weeks between pre- menopausal and post- menopausal groups adipose triglyceride lipase (ATGL), monoacylglycerol lipase (MGL) and perilipin (PLIN) protein levels were significantly lower in the post- menopausal group than in the pre- menopausal group. Hormone-sensitive lipase (HSL) protein levels were significantly higher in the post- menopausal group than in the pre- menopausal group. In addition, leptin concentration was significantly decreased after resistance exercise in the post- menopausal group. Adiponectin concentration was significantly increased after resistance exercise in the both groups. Conclusions: This study indicates that regular resistance exercise to change of leptin and adiponectin might be release of reduction of % fat, and driving overall greater change ATGL, HSL, MGL and PLIN levels in subcutaneous fatty tissue in the obese post- menopausal group more than obese pre- menopausal group.

scholarly journals Effects of regulatory exercise on lipolysis pathway in obese pre and postmenopausal women

2020 ◽  
Author(s):  
Kyu-Min Park ◽  
Seung-Taek Lim ◽  
Kun-Young Sung ◽  
Sunghwun Kang
Keyword(s):  
Protein Expression ◽  
Resistance Exercise ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fatty Tissue ◽  
Serum Samples ◽  
Resistance Exercise Training ◽  
Menopausal Women ◽  
Fitness Tests ◽  
Subcutaneous Fatty Tissue ◽  
Post Menopausal

Abstract Background The purpose of study was to examine the effects of regulatory resistance exercise for 12 weeks on lipolysis pathway in pre- and post- menopausal women with obesity. Methods Twenty-three pre- and post- menopausal women with body fat percentages of 30% or more divided into pre- menopausal group (n = 9) and post- menopausal group (n = 14). All subjects participated in resistance exercise training for 12 weeks. All participant’s anthropometric measurements and physical fitness tests were performed. Protein analyses were performed with subcutaneous fatty tissue extracted, and the samples were analyzed of relevant protein expression changes by using Western blotting. All serum samples were submitted for enzyme-linked immunosorbent assay measurements of adipocyte factors. Results After 12 weeks between pre- menopausal and post- menopausal groups ATGL, MGL and PLIN protein expression were significantly lower in the post- menopausal group than in the pre- menopausal group. HSL protein expression were significantly higher in the post- menopausal group than in the pre- menopausal group. In addition, leptin concentration was significantly decreased, and adiponectin concentration was significantly increased after resistance exercise in the post- menopausal group more than pre- menopausal group. Conclusions In this study indicates that regular resistance exercise to change of leptin and adiponectin might be release of overall decreased ATGL, HSL, MGL and PLIN expression in subcutaneous fatty tissue, and driving reduction of % fat in the obese post- menopausal group more than obese pre- menopausal group.

scholarly journals Effects of regulatory exercise on lipolysis pathway in obese pre and postmenopausal women

2020 ◽  
Author(s):  
Kyu-Min Park ◽  
Seung-Taek Lim ◽  
Kun-Young Sung ◽  
Sunghwun Kang
Keyword(s):  
Protein Expression ◽  
Resistance Exercise ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fatty Tissue ◽  
Serum Samples ◽  
Resistance Exercise Training ◽  
Menopausal Women ◽  
Fitness Tests ◽  
Subcutaneous Fatty Tissue ◽  
Post Menopausal

Abstract Background and objectives: The purpose of study was to examine the effects of regulatory resistance exercise for 12 weeks on lipolysis pathway in pre- and post- menopausal women with obesity. Methods: Twenty-three pre- and post- menopausal women with body fat percentages of 30% or more divided into pre- menopausal group (n=9) and post- menopausal group (n=14). All subjects participated in resistance exercise training for 12 weeks. All participant’s anthropometric measurements and physical fitness tests were performed. Protein analyses were performed with subcutaneous fatty tissue extracted, and the samples were analyzed of relevant protein expression changes by using Western blotting. All serum samples were submitted for enzyme-linked immunosorbent assay measurements of adipocyte factors. Results: After 12 weeks between pre- menopausal and post- menopausal groups ATGL, MGL and PLIN protein expression were significantly lower in the post- menopausal group than in the pre- menopausal group. HSL protein expression were significantly higher in the post- menopausal group than in the pre- menopausal group. In addition, leptin concentration was significantly decreased, and adiponectin concentration was significantly increased after resistance exercise in the post- menopausal group more than pre- menopausal group. Conclusions: In this study indicates that regular resistance exercise to change of leptin and adiponectin might be release of overall decreased ATGL, HSL, MGL and PLIN expression in subcutaneous fatty tissue, and driving reduction of % fat in the obese post- menopausal group more than obese pre- menopausal group.

scholarly journals Effect of Resistance Exercise on the Lipolysis Pathway in Obese Pre- and Postmenopausal Women

2021 ◽  
Vol 11 (9) ◽  
pp. 874
Author(s):  
Sunghwun Kang ◽  
Kyu-Min Park ◽  
Kun-Young Sung ◽  
Yuning Yuan ◽  
Seung-Taek Lim
Keyword(s):  
Adipose Tissue ◽  
Resistance Exercise ◽  
Postmenopausal Women ◽  
Body Fat ◽  
Lipolytic Activity ◽  
Premenopausal Women ◽  
Hormone Sensitive Lipase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fatty Tissue ◽  
Protein Levels

Physical exercise may stimulate lipolytic activity within adipose tissue. Furthermore, resistance exercise may contribute to the more efficient reduction in adipose tissue mass and prevent the accumulation thereof in obese women. The purpose of this study was to examine the effects of regular resistance exercise for 12 weeks on the lipolysis pathway in women with obesity. Twenty-three pre- and postmenopausal women with body fat percentages of 30% or more were divided into the premenopausal group (n = 9) and the postmenopausal group (n = 14). All subjects participated in resistance exercise training for 12 weeks. Anthropometric and physical fitness tests were performed on all participants. Protein analyses were performed on extracted subcutaneous fatty tissue, and changes in the relevant protein levels in the samples were analyzed by Western blotting. All serum samples were submitted for enzyme-linked immunosorbent assay measurements of adipocyte factors. After 12 weeks, the adipose triglyceride lipase, monoacylglycerol lipase, and perilipin1 protein levels were significantly lower in the postmenopausal group than in the premenopausal group. The hormone-sensitive lipase protein levels were significantly higher in the postmenopausal group than in the premenopausal group. In addition, leptin concentrations were significantly decreased after resistance exercise in the postmenopausal group. Adiponectin concentrations were significantly increased after resistance exercise in both groups. These findings indicate that regular resistance exercise is effective in reducing the weight and body fat of obese premenopausal women, and in the secretion of adiponectin. On the other hand, postmenopausal women were found to have redeced weight and body fat, and were found to be positive for the secretion of adipokine factors. In addition, positive changes in lipolysis pathway factors in adipose tissue promote lipid degradation and reduce fat mass. Thus, regular resistance exercise shows positive changes in the lipolysis pathway more effectively in weight and body fat reduction in postmenopausal women than in premenopausal women.

scholarly journals miR-128-3p inhibits apoptosis and inflammation in LPS-induced sepsis by targeting TGFBR2

Open Medicine ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. 274-283
Author(s):  
Peng Yang ◽  
Jianhua Han ◽  
Shigeng Li ◽  
Shaoning Luo ◽  
Xusheng Tu ◽  
...  
Keyword(s):  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Quantitative Polymerase Chain Reaction ◽  
Luciferase Reporter ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Aberrant Expression ◽  
Protein Levels ◽  
Apoptosis And Inflammation ◽  
Hk2 Cells

Abstract Background Sepsis is a systemic inflammatory response that can lead to the dysfunction of many organs. The aberrant expression of miRNAs is associated with the pathogenesis of sepsis. However, the biological functions of miR-128-3p in sepsis remain largely unknown, and its mechanism should be further investigated. This study aimed to determine the regulatory network of miR-128-3p and TGFBR2 in lipopolysaccharide (LPS)-induced sepsis. Methods The expression levels of miR-128-3p and transforming growth factor beta receptors II (TGFBR2) were detected by quantitative polymerase chain reaction (qPCR). The protein levels of TGFBR2, Bcl-2, Bax, cleaved caspase 3, Smad2, and Smad3 were measured by western blot. Cell apoptosis was analyzed by flow cytometry. Cytokine production was detected by enzyme-linked immunosorbent assay (ELISA). The binding sites of miR-128-3p and TGFBR2 were predicted by Targetscan online software and confirmed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. Results The level of miR-128-3p was decreased, and TGFBR2 expression was increased in serum samples of sepsis patients and LPS-induced HK2 cells. Overexpression of miR-128-3p or knockdown of TGFBR2 ameliorated LPS-induced inflammation and apoptosis. Moreover, TGFBR2 was a direct target of miR-128-3p, and its overexpression reversed the inhibitory effects of miR-128-3p overexpression on inflammation and apoptosis in LPS-induced HK2 cells. Besides, overexpression of miR-128-3p downregulated TGFBR2 to suppress the activation of the Smad signaling pathway. Conclusion miR-128-3p could inhibit apoptosis and inflammation by targeting TGFBR2 in LPS-induced HK2 cells, which might provide therapeutic strategy for the treatment of sepsis.

scholarly journals Relationship between serum-based biomarkers & Magnetic Resonance Imaging measures following mild traumatic brain injury in collegiate athletes post return to play

Neurology ◽  
2019 ◽  
Vol 93 (14 Supplement 1) ◽  
pp. S25.1-S25
Author(s):  
Taylor Susa ◽  
Marguerite Moore ◽  
Joshua Carlson
Keyword(s):  
Gray Matter Volume ◽  
Collegiate Athletes ◽  
Positive Association ◽  
Time Frame ◽  
Return To Play ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Serum Samples ◽  
Protein Levels ◽  
Mri Scans

ObjectiveThis study analyzed MRI and serum samples from 30 participants across two groups to explore the relationship between protein levels and MRI scans in post return-to-play collegiate athletes following concussion.BackgroundRecently, there has been an increase in concussion research on their effects on different protein levels in serum (a derived portion of blood) between concussed and control groups. Recent research examining serum biomarkers in concussion have found elevated levels of many proteins, but overall have mixed results in correlation with MRI. However, these studies have not focused on the lingering effects that exist in post return-to-play.Design/MethodsThe first group (n = 15) consisted of recently cleared to return-to-play collegiate athletes after experiencing a sports-related concussion. The second group (n = 15) was collegiate athlete controls matched on age, sex, and sport. Serum samples were collected to assess the levels of proteins following post return-to-play. These proteins were evaluated using Enzyme-Linked Immunosorbent Assay kits (ELIASA).ResultsAn overall BDNF effect was observed between groups (p < 0.05), the concussed group exhibited significantly higher levels of serum BDNF compared to the control group. A positive association between BDNF and gray matter volume (GMV) was observed at a 250 voxel cluster level in both the right (pFDR = 0.015) and left cerebellum region (pFDR = 0.045) across groups. A negative association between BDNF and GMV in both groups was observed in the brainstem (p = 0.029) and the precuneus (p = 0.017) areas. A differential relationship between group and BDNF on GMV was observed (p = 0.022) in the prefrontal cortex.ConclusionsPrevious research has not examined the post return-to-play effects in neuroplasticity specific proteins, nor the time frame of injury in comparison to controls with MRI. Serum-based biomarkers and MRI grant a better depiction of what is occurring during post return-to-play.

scholarly journals Effects of the Human Immunodeficiency Virus-Protease Inhibitor, Ritonavir, on Basal and Catecholamine-Stimulated Lipolysis

2005 ◽  
Vol 90 (6) ◽  
pp. 3251-3261 ◽  
Author(s):  
Diane C. Adler-Wailes ◽  
Hanguan Liu ◽  
Faiyaz Ahmad ◽  
Ningping Feng ◽  
Constantine Londos ◽  
...  
Keyword(s):  
Protein Expression ◽  
Protease Inhibitor ◽  
Hormone Sensitive Lipase ◽  
Hiv Protease ◽  
Intracellular Camp ◽  
Cellular Mechanisms ◽  
Protein Levels ◽  
Immunodeficiency Virus ◽  
Hormone Sensitive ◽  
Phenylisopropyl Adenosine

Several of the aspartic acid protease inhibitors used to treat HIV infection increase basal lipolysis in adipocytes, but the cellular mechanisms leading to this augmentation are not well understood. We therefore studied the effects of chronic exposure to the HIV protease inhibitor, ritonavir, on the lipolytic cascade in 3T3-L1 adipocytes. Treatment of 3T3-L1 adipocytes with ritonavir for 14 d (during and after differentiation) enhanced basal, isoproterenol (Iso)-stimulated, and cAMP analog-stimulated lipolysis. Enhancement of lipolysis was observed after Iso at concentrations between 0.1 and 10 μm. Despite a significant decrease in cyclic nucleotide phosphodiesterase (PDE)3B activity and protein levels, there were no changes in Iso-stimulated intracellular cAMP, protein kinase A (PKA) expression, or PKA activity. Ritonavir-augmented lipolysis was also observed under conditions that reversed the effect on PDE3B activity via preincubation with 1 μm (-)-N6-(2-phenylisopropyl)adenosine. In ritonavir-treated cells, protein expression of the lipid droplet-protective protein, perilipin, was significantly decreased, whereas there was no change in hormone-sensitive lipase. Activation of ERK1/2 by Iso did not play a role in the augmentation. We conclude that ritonavir decreases PDE3B and perilipin protein expression and affects both basal and catecholamine-stimulated lipolysis in 3T3-L1 adipocytes primarily through actions at sites downstream of PKA.

Fasting upregulates adipose triglyceride lipase and hormone-sensitive lipase levels and phosphorylation in mouse kidney

2015 ◽  
Vol 93 (3) ◽  
pp. 262-267 ◽  
Author(s):  
Phillip M. Marvyn ◽  
Ryan M. Bradley ◽  
Emily B. Button ◽  
Emily B. Mardian ◽  
Robin E. Duncan
Keyword(s):  
Fatty Acids ◽  
Hormone Sensitive Lipase ◽  
Binding Motif ◽  
Adipose Triglyceride Lipase ◽  
Triglyceride Lipase ◽  
Protein Levels ◽  
Fasted State ◽  
Esterified Fatty Acids ◽  
Glomerular Filtrate ◽  
Hormone Sensitive

Circulating non-esterified fatty acids (NEFA) rise during fasting and are taken up by the kidneys, either directly from the plasma or during re-uptake of albumin from glomerular filtrate, and are stored as triacylglycerol (TAG). Subsequent utilization of stored fatty acids requires their hydrolytic release from cellular lipid droplets, but relatively little is known about renal lipolysis. We found that total [3H]triolein hydrolase activity of kidney lysates was significantly increased by 15% in the fasted state. Adipose triglyceride lipase (Atgl) and hormone-sensitive lipase (Hsl) mRNA expression was time-dependently increased by fasting, along with other fatty acid metabolism genes (Pparα, Cd36, and Aox). ATGL and HSL protein levels were also significantly induced (by 239 ± 7% and 322 ± 8%, respectively). Concomitant with changes in total protein levels, there was an increase in ATGL phosphorylation at the AMPK-regulated serine 406 site in the 14-3-3 binding motif, and an increase in HSL phosphorylation at serines 565 and 660 that are regulated by AMPK and PKA, respectively. Using immunofluorescence, we further demonstrate nearly ubiquitous expression of ATGL in the renal cortex with a concentration on the apical/lumenal surface of some cortical tubules. Our findings suggest a role for ATGL and HSL in kidney lipolysis.

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