scholarly journals Adsorbed Leucaena Protein on Citrate Modified Fe3O4 Nanoparticles: Synthesis, Characterization, and Its Application As Magnetic Coagulant

2020 ◽  
Author(s):  
Hans Kristianto ◽  
Edwin Reynaldi ◽  
Susiana Prasetyo ◽  
Asaf K Sugih
Keyword(s):  
Protein Adsorption ◽  
Leucaena Leucocephala ◽  
Lower Cost ◽  
Trisodium Citrate ◽  
Settling Time ◽  
Citrate Concentration ◽  
Adsorbed Protein ◽  
Coagulant Dosage ◽  
Before And After ◽  
Natural Coagulants

Abstract Natural coagulants from plants resources have gained a lot of attention as it is renewable, biodegradable, non-hazardous, lower cost, and less sludge generated compared to chemical coagulants. However there are still some drawbacks, namely long settling time and possible increase of dissolved organic carbon (DOC) in the treated water. In this paper we tried to address these drawbacks by utilizing citrate modified Fe3O4 to adsorb protein from Leucaena leucocephala as the active coagulating agent. The effect of trisodium citrate concentration and protein adsorption pH to the adsorbed protein was investigated. It was found that the trisodium citrate concentration of 0.5 M and pH 4.0 gave the highest protein adsorption. The obtained magnetic coagulant was furthermore characterized using SEM, XRD, FTIR, and TEM, to observe the characteristics before and after protein adsorption. Furthermore the effect of pH (2-10) and coagulant dosage (60-600 mg. L-1) to the removal of synthetic Congo red wastewater and sludge volume formation was investigated. It was found that pH 3.0 was the best pH for coagulation due to charge neutralization mechanism of leucaena protein. Furthermore the highest removal was obtained at dosage 420mg. L-1 with 80% removal. This result was comparable with crude extract of leucaena with half settling time (20 min) and lower increase of permanganate value.

scholarly journals Adsorbed Leucaena protein on citrate modified Fe3O4 nanoparticles: synthesis, characterization, and its application as magnetic coagulant

2020 ◽  
Author(s):  
Hans Kristianto ◽  
Edwin Reynaldi ◽  
Susiana Prasetyo ◽  
Asaf K Sugih
Keyword(s):  
Protein Adsorption ◽  
Lower Cost ◽  
Trisodium Citrate ◽  
Settling Time ◽  
Treated Water ◽  
Citrate Concentration ◽  
Dissolved Organics ◽  
Coagulant Dosage ◽  
Before And After ◽  
Natural Coagulants

Abstract Natural coagulants from plants resources have gained a lot of attention as it is renewable, biodegradable, non-hazardous, lower cost, and less sludge generated compared to chemical coagulants. However there are still some drawbacks, namely long settling time and possible increase of dissolved organic carbon in the treated water. In this paper we tried to address these drawbacks by utilizing citrate modified Fe3O4 to adsorb protein from Leucaena leucocephala as the active coagulating agent. The effect of trisodium citrate concentration and protein adsorption pH to the adsorbed protein was investigated. It was found that the trisodium citrate concentration of 0.5 M and pH 4.0 gave the highest protein adsorption. The obtained magnetic coagulant was furthermore characterized using SEM, XRD, FTIR, and TEM, to observe the characteristics before and after protein adsorption. Furthermore the effect of pH (2.0 to 10.0) and coagulant dosage (60 to 600 mg L-1) to the removal of synthetic Congo red wastewater and sludge volume formation was investigated. It was found that pH 3.0 was the best pH for coagulation due to charge neutralization mechanism of leucaena protein. Furthermore the highest removal was obtained at dosage 420 mg L-1 with 80% removal. This result was comparable with crude extract of leucaena with half settling time (20 min) and lower increase of permanganate value, indicating lower increase of dissolved organics in the treated water.

scholarly journals Adsorbed Leucaena protein on citrate modified Fe3O4 nanoparticles: synthesis, characterization, and its application as magnetic coagulant

2020 ◽  
Author(s):  
Hans Kristianto ◽  
Edwin Reynaldi ◽  
Susiana Prasetyo ◽  
Asaf K Sugih
Keyword(s):  
Protein Adsorption ◽  
Lower Cost ◽  
Trisodium Citrate ◽  
Settling Time ◽  
Treated Water ◽  
Citrate Concentration ◽  
Dissolved Organics ◽  
Coagulant Dosage ◽  
Before And After ◽  
Natural Coagulants

Abstract Natural coagulants from plants resources have gained a lot of attention as it is renewable, biodegradable, non-hazardous, lower cost, and less sludge generated compared to chemical coagulants. However there are still some drawbacks, namely long settling time and possible increase of dissolved organic carbon in the treated water. In this paper we tried to address these drawbacks by utilizing citrate modified Fe3O4 to adsorb protein from Leucaena leucocephala as the active coagulating agent. The effect of trisodium citrate concentration and protein adsorption pH to the adsorbed protein was investigated. It was found that the trisodium citrate concentration of 0.5 M and pH 4.0 gave the highest protein adsorption. The obtained magnetic coagulant was furthermore characterized using SEM, XRD, FTIR, and TEM, to observe the characteristics before and after protein adsorption. Furthermore the effect of pH (2.0 to 10.0) and coagulant dosage (60 to 600 mg L-1) to the removal of synthetic Congo red wastewater and sludge volume formation was investigated. It was found that pH 3.0 was the best pH for coagulation due to charge neutralization mechanism of leucaena protein. Furthermore the highest removal was obtained at dosage 420 mg L-1 with 80% removal. This result was comparable with crude extract of leucaena with half settling time (20 min) and lower increase of permanganate value, indicating lower increase of dissolved organics in the treated water.

scholarly journals Adsorbed leucaena protein on citrate modified Fe3O4 nanoparticles: synthesis, characterization, and its application as magnetic coagulant

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Hans Kristianto ◽  
Edwin Reynaldi ◽  
Susiana Prasetyo ◽  
Asaf K. Sugih
Keyword(s):  
Electron Microscopy ◽  
Protein Adsorption ◽  
Trisodium Citrate ◽  
Settling Time ◽  
Treated Water ◽  
Citrate Concentration ◽  
Transmission Electron ◽  
Dissolved Organics ◽  
Coagulant Dosage ◽  
Before And After

AbstractNatural coagulants from plants resources have gained a lot of attention as it is renewable, biodegradable, non-hazardous, lower cost, and less sludge generated compared to chemical coagulants. However there are still some drawbacks, namely long settling time and possible increase of dissolved organic carbon in the treated water. In this paper we tried to address these drawbacks by utilizing citrate modified Fe3O4 to adsorb protein from Leucaena leucocephala as the active coagulating agent. The effect of trisodium citrate concentration and protein adsorption pH to the adsorbed protein was investigated. It was found that the trisodium citrate concentration of 0.5 M and pH 4.0 gave the highest protein adsorption. The obtained magnetic coagulant was furthermore characterized using Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared Spectroscopy, and Transmission Electron Microscopy to observe the characteristics before and after protein adsorption. Furthermore, the effect of pH (2 to 10) and coagulant dosage (60 to 600 mg L− 1) to the removal of synthetic Congo red wastewater and sludge volume formation was investigated. It was found that pH 3 was the best pH for coagulation due to charge neutralization mechanism of leucaena protein. Furthermore the highest removal was obtained at dosage 420 mg L− 1 with 80% removal. This result was comparable with crude extract of leucaena with half settling time (20 min) and lower increase of permanganate value, indicating lower increase of dissolved organics in the treated water.

scholarly journals Adsorbed leucaena protein on citrate modified Fe3O4 nanoparticles: synthesis, characterization, and its application as magnetic coagulant

2020 ◽  
Author(s):  
Hans Kristianto ◽  
Edwin Reynaldi ◽  
Susiana Prasetyo ◽  
Asaf K Sugih
Keyword(s):  
Electron Microscopy ◽  
Protein Adsorption ◽  
Trisodium Citrate ◽  
Settling Time ◽  
Treated Water ◽  
Citrate Concentration ◽  
Transmission Electron ◽  
Dissolved Organics ◽  
Coagulant Dosage ◽  
Before And After

Abstract Natural coagulants from plants resources have gained a lot of attention as it is renewable, biodegradable, non-hazardous, lower cost, and less sludge generated compared to chemical coagulants. However there are still some drawbacks, namely long settling time and possible increase of dissolved organic carbon in the treated water. In this paper we tried to address these drawbacks by utilizing citrate modified Fe3O4 to adsorb protein from Leucaena leucocephala as the active coagulating agent. The effect of trisodium citrate concentration and protein adsorption pH to the adsorbed protein was investigated. It was found that the trisodium citrate concentration of 0.5 M and pH 4.0 gave the highest protein adsorption. The obtained magnetic coagulant was furthermore characterized using Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared Spectroscopy, and Transmission Electron Microscopy to observe the characteristics before and after protein adsorption. Furthermore, the effect of pH (2 to 10) and coagulant dosage (60 to 600 mg L-1) to the removal of synthetic Congo red wastewater and sludge volume formation was investigated. It was found that pH 3 was the best pH for coagulation due to charge neutralization mechanism of leucaena protein. Furthermore the highest removal was obtained at dosage 420 mg L-1 with 80% removal. This result was comparable with crude extract of leucaena with half settling time (20 min) and lower increase of permanganate value, indicating lower increase of dissolved organics in the treated water.

A look into the interaction of metal oxide thin films with biological media: Albumin and Fibrinogen adsorption

2012 ◽  
Vol 1376 ◽  
Author(s):  
P. Silva-Bermudez ◽  
S. Muhl ◽  
M. Rivera ◽  
S. E. Rodil
Keyword(s):  
Surface Roughness ◽  
Surface Energy ◽  
Protein Adsorption ◽  
Photoelectron Spectroscopy ◽  
Ex Situ ◽  
Polar Component ◽  
Metal Oxide Thin Films ◽  
Adsorbed Protein ◽  
Major Chemical ◽  
Fibrinogen Adsorption

ABSTRACTIn the present work, the adsorption of albumin and fibrinogen on Ta, Nb, Ti and Zr oxidesthin films deposited on Si (100) wafers by magnetron sputtering was studied in order to get a better understanding of the correlation among the surface properties of these oxides and the protein adsorption phenomena on their surfaces. The surface energy, hydrophobicity, chemical composition, roughness and atomic order of the films were characterized. The films were immersedfor 45 minutes in single protein solutions; either albumin or fibrinogenand the adsorbed protein layer on the films was studied ex-situ in a dry ambient using bothX-ray photoelectron spectroscopy and atomic force microscopy.The adsorption of albumin and fibrinogen on the films modified the surface morphology and decreased the surface roughness for all the four different metal oxides. The XPS results confirmed the presence of the protein on the surface of the films and showed that the two proteins studied were adsorbed without undergoing a major chemical decomposition. A correlation between the surface roughness,the polar component of the surface energy of the films and the atomic percentage of nitrogen on the films after protein adsorption, an indirect signal of the amount of protein adsorbed, was found for albumin and fibrinogen adsorption on Ta, Nb and Ti oxides; the largest the roughness or the polar component the largest amount of adsorbed protein.

scholarly journals Performance of Natural Coagulants in the Treatment of Vegetable Oil Industrial Effluent

2020 ◽  
pp. 12-21
Author(s):  
C. F. Okey- Onyesolu ◽  
C. C. Okoye ◽  
C. N. Ude ◽  
O. D. Onukwuli ◽  
O. E. Achugbu
Keyword(s):  
Vegetable Oil ◽  
Industrial Wastewater ◽  
Industrial Effluent ◽  
Chemical Properties ◽  
Proximate Analysis ◽  
Chemical Compositions ◽  
Fish Scale ◽  
Coagulant Dosage ◽  
Natural Coagulants ◽  
Egg Shell

The potentials of natural coagulants (egg shell and fish scale) in the treatment of vegetable oil industrial wastewater were investigated. The coagulants were characterized to determine their chemical compositions, functional groups and morphology using proximate analysis method, Fourier Infra-red spectroscope (FTIR) and Scanning Electron Microscope (SEM) respectively. The industrial wastewater was equally characterized to determine its heavy metal composition and physico-chemical properties. The effects of various process parameters such as pH, coagulants dosage, time and temperature were examined during the treatment of the waste water using the natural coagulants. The proximate analysis shows that Fish Scale Coagulant (FSC) contains more carbohydrate, 42.30% while Egg Shell Coagulant (ESC) has protein content of 39.65%. It was discovered that the coagulating properties of both FSC and ESC improve after extraction due to presence of isocyanates, isothiocyadilimides, azides, ketenes, aldehydes and ketone. The turbidity of the wastewater was very high (365NTU) above the WHO recommended value. The process variables have significant effects on the coagulation with best removal efficiency of 88.54% for ESC and 74.12% for FSC at pH of 4.0 (ESC) and pH of 7.0(FSC); 0.5 g/L (ESC) and 0.04 g/L (FSC) coagulant dosage and temperature of 40°C. Therefore, both coagulants are effective in treating vegetable oil effluent but egg shell coagulant was found to be a better coagulant. Further research on optimization, kinetics and thermodynamics of the coagulation process using ESC and FSC should be carried out.

scholarly journals Variation in the prices of oncology medicines across Europe and the implications for the future

2021 ◽  
Vol 10 (2) ◽  
pp. 72-82
Author(s):  
Brian Godman ◽  
Steven Simoens ◽  
Amanj Kurdi ◽  
Gisbert Selke ◽  
John Yfantopoulos ◽  
...  
Keyword(s):  
Population Size ◽  
Patent Protection ◽  
Lower Cost ◽  
European Countries ◽  
Health Authorities ◽  
Before And After ◽  
Per Capita ◽  
Oral Oncology ◽  
Over Time ◽  
Ageing Populations

Introduction/Objectives: Health authorities are facing increasing challenges to the sustainability of their healthcare systems because of the growing expenditures on medicines, including new, high-priced oncology medicines, and changes in disease prevalence in their ageing populations. Medicine prices in European countries are greatly affected by the ability to negotiate reasonable prices. Concerns have been expressed that prices of patented medicines do not fall sufficiently after the introduction of lower-cost generic oncology medicines. The objective of this study was to examine the associations over time in selected European countries between the prices of oral oncology medicines, population size, and gross domestic product (GDP) before and after the introduction of generic versions. Evidence of periodic reassessments of the price, value, and place in treatment of these medicines was also looked for. The goal of this review was to stimulate debate about possible improvements in approaches to reimbursement negotiations. Methodology: Analysis was performed of reimbursed prices of three oral oncology medicines (imatinib, erlotinib and fludarabine) between 2013 and 2017 across Europe. Correlations were explored between GDP, population size, and prices. Findings were compared with previous research regarding prices of generic oral oncology medicines. Results: The prices of imatinib, erlotinib and fludarabine varied among European countries, and there was limited price erosion over time in the absence of generics. There appeared to be no correlation between population size and price, but higher prices of on-patent oral cancer medicines were seen among countries with higher GDP per capita. Conclusion: Limited price erosion for patented medicines contributed to increases in oncology medicine budgets across the region. There was also a concerning lack of evidence re-assessments of the price, value, and place in treatment of patented oncology medicines following the loss of patent protection of standard medicines. The use of such proactive re-assessments in negotiating tactics might positively impact global expenditures for oncology medicines.

Sensing of Protein Adsorption by Composites Consisting of Silver Nanoparticles and Hydroxyapatite

2010 ◽  
Vol 76 ◽  
pp. 90-99
Author(s):  
Chikara Ohtsuki ◽  
Yuji Ichikawa ◽  
Hiroyuki Shibata ◽  
Tsukasa Torimoto ◽  
Ill Yong Kim
Keyword(s):  
Silver Nanoparticles ◽  
Protein Adsorption ◽  
Phosphate Buffer Solution ◽  
Trisodium Citrate ◽  
Localized Surface Plasmon ◽  
Surface Charges ◽  
Buffer Solution ◽  
Promising Candidate ◽  
Hydroxyapatite Powder ◽  
The Matrix

The sensing of protein adsorption by silver nanoparticles/hydroxyapatite composites was investigated using a phosphate buffer solution containing bovine serum albumin (BSA) or lysozyme (LSZ). The adsorption of BSA and LSZ on the composites prepared without using trisodium citrate was similar to plain hydroxyapatite, whereas composites prepared with trisodium citrate showed lower BSA adsorption and higher LSZ adsorption than plain hydroxyapatite powder. Because the ability of the adsorption is mainly governed by surface charges of the powders, the usage of trisodium citrate during the preparation is assumed to produce high negative charges on the surface. The protein adsorption resulted in peak shifts in localized surface plasmon resonance (LSPR) spectra. The peak shifts clearly corresponded to the concentration of the surrounding proteins up to the point of saturation of adsorption on the hydroxyapatite. The silver nanoparticles/hydroxyapatite composites are promising candidate materials for detection of protein adsorption by measurement of LSPR peak shifts, that may be attributed to changes in the dielectric properties of the matrix fluid surrounding the silver nanoparticles.

Preparation of Cellulose Acetate Ultra-Fine Fibers Containing Quasi-Spherical Gold Nanoparticles by Electrospinning Method

2011 ◽  
Vol 391-392 ◽  
pp. 400-403
Author(s):  
Dong Mei Zhao ◽  
Li Guo Sun ◽  
Li Li Lv ◽  
Jian Li
Keyword(s):  
Electron Microscopy ◽  
Gold Nanoparticles ◽  
Cellulose Acetate ◽  
Trisodium Citrate ◽  
X Ray Diffraction ◽  
Au Nps ◽  
Transmission Electron ◽  
Electrospinning Method ◽  
Before And After ◽  
Spherical Gold Nanoparticles

Quasi-spherical gold nanoparticles(Au NPs) prepared by trisodium citrate reduction of HAuCl4were dispersed into cellulose acetate(CA) ultra-fine fibers by electrospinning. The optical properties of Au NPs before and after electrospinning were measured by UV-vis spectrometer. The morphology and distribution of Au NPs in CA ultra-fine fibers were observed by transmission electron microscopy (TEM). The morphology and diameter of Au NPs/CA fibers were studied by scanning electron microscopy (SEM). The crystallinity change of CA fiber before and after adding Au NPs was characterized by X-ray diffraction (XRD).

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