scholarly journals Parallel analysis of global garlic gene expression and alliin content following leaf wounding

2020 ◽  
Author(s):  
Xuqin Yang ◽  
Yiren SU ◽  
jiaying wu ◽  
wen wan ◽  
Huijian chen ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Allium Sativum ◽  
Biosynthetic Pathways ◽  
Important Food ◽  
Transcriptomic Data ◽  
Mature Leaves ◽  
Economic Significance ◽  
Different Parts ◽  
Over Time

Abstract Background Allium sativum (garlic) is both an important food and medicinal plant of economic significance. This plant is rich in sulfides, especially alliin, which is a precursor for the synthesis of allicin. At present, there are few reports on the determination of alliin content in different parts of garlic under abiotic stress. Results Our data determining the accumulation of alliin in different organs showed that the content of alliin in garlic root was the lowest level recorded, while the content of alliin within a garlic bud was the highest level determined. Further, alliin levels decreased in mature leaves following wounding. Further, transcriptomic data generated over time following wounding of mature garlic leaves showed genes integral to the biosynthetic pathways of cysteine (CYS) and serine (SER) formation were significantly up-regulated. Conclusions This differential expression could underpin the accumulation of alliin and its precursors in garlic. Thus, our results provide a platform to help elucidate the biosynthetic pathway alliin biosynthesis.

scholarly journals Parallel analysis of global garlic gene expression and alliin content following leaf wounding

2020 ◽  
Author(s):  
Xuqin Yang ◽  
Yiren SU ◽  
jiaying wu ◽  
wen wan ◽  
Huijian chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Food Source ◽  
Allium Sativum ◽  
Parallel Analysis ◽  
Biosynthetic Pathways ◽  
Transcriptome Data ◽  
Important Food ◽  
Mature Leaves ◽  
Important Food Source ◽  
Over Time

Abstract Background: Allium sativum (garlic) is an economically important food source and medicinal plant rich in sulfides and other protective substances such as alliin, the precursor of allicin biosynthesis. Cysteine, serine and sulfur is the precursor of alliin biosynthesis. However, little is known about the alliin content under abiotic stress or the mechanism by which its synthesized. Results: The findings revealed that the content of alliin was lowest in the garlic roots, and highest in the buds. Furthermore, alliin levels decreased in mature leaves following wounding. Transcriptome data generated over time after wounding further revealed significant up-regulation of genes integral to the biosynthetic pathways of cysteine and serine in mature garlic leaves. Conclusions: The findings suggest that differential expression of cysteine, serine and sulfide-related genes underlies the accumulation of alliin and its precursors in garlic, providing a basis for further analyses of alliin biosynthesis.

scholarly journals Parallel analysis of global garlic gene expression and alliin content following leaf wounding

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Xuqin Yang ◽  
Yiren Su ◽  
Jiaying Wu ◽  
Wen Wan ◽  
Huijian Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Food Source ◽  
Allium Sativum ◽  
Parallel Analysis ◽  
Biosynthetic Pathways ◽  
Transcriptome Data ◽  
Important Food ◽  
Mature Leaves ◽  
Important Food Source ◽  
Over Time

Abstract Background Allium sativum (garlic) is an economically important food source and medicinal plant rich in sulfides and other protective substances such as alliin, the precursor of allicin biosynthesis. Cysteine, serine and sulfur is the precursor of alliin biosynthesis. However, little is known about the alliin content under abiotic stress or the mechanism by which it is synthesized. Results The findings revealed that the content of alliin was lowest in the garlic roots, and highest in the buds. Furthermore, alliin levels decreased in mature leaves following wounding. Transcriptome data generated over time after wounding further revealed significant up-regulation of genes integral to the biosynthetic pathways of cysteine and serine in mature garlic leaves. Conclusions The findings suggest that differential expression of cysteine, serine and sulfide-related genes underlies the accumulation of alliin and its precursors in garlic, providing a basis for further analyses of alliin biosynthesis.

Determination of Eleutherosides and β-Glucan Content from Different Parts and Cultivating Areas of A. senticosus and A. koreanum

2013 ◽  
Vol 42 (12) ◽  
pp. 2082-2087 ◽  
Author(s):  
Young-Hyun Kim ◽  
Da-Bin Bae ◽  
Jong Seok Lee ◽  
Sun-Ok Park ◽  
Sang-Jong Lee ◽  
...  
Keyword(s):  
Different Parts

scholarly journals Expression Level of Mature miR172 in Wild Type and StSUT4-Silenced Plants of Solanum tuberosum Is Sucrose-Dependent

2021 ◽  
Vol 22 (3) ◽  
pp. 1455
Author(s):  
Varsha Garg ◽  
Aleksandra Hackel ◽  
Christina Kühn
Keyword(s):  
Solanum Tuberosum ◽  
Wild Type ◽  
Potato Plants ◽  
Mature Leaves ◽  
In Vitro Plantlets ◽  
Long Time ◽  
Short Time ◽  
Cut Leaves

In potato plants, the phloem-mobile miR172 is involved in the sugar-dependent transmission of flower and tuber inducing signal transduction pathways and a clear link between solute transport and the induction of flowering and tuberization was demonstrated. The sucrose transporter StSUT4 seems to play an important role in the photoperiod-dependent triggering of both developmental processes, flowering and tuberization, and the phenotype of StSUT4-inhibited potato plants is reminiscent to miR172 overexpressing plants. The first aim of this study was the determination of the level of miR172 in sink and source leaves of StSUT4-silenced as well as StSUT4-overexpressing plants in comparison to Solanum tuberosum ssp. Andigena wild type plants. The second aim was to investigate the effect of sugars on the level of miRNA172 in whole cut leaves, as well as in whole in vitro plantlets that were supplemented with exogenous sugars. Experiments clearly show a sucrose-dependent induction of the level of mature miR172 in short time as well as long time experiments. A sucrose-dependent accumulation of miR172 was also measured in mature leaves of StSUT4-silenced plants where sucrose export is delayed and sucrose accumulates at the end of the light period.

Parametric-Based Determination of Cylindrical Variations in Geometric Tolerancing

1999 ◽  
Vol 122 (3) ◽  
pp. 549-555 ◽  
Author(s):  
C. Carolina Ba´rcenas ◽  
Paul M. Griffin
Keyword(s):  
Geometric Dimensioning And Tolerancing ◽  
Geometric Tolerancing ◽  
Dimensioning And Tolerancing ◽  
Surface Variation ◽  
Cylindrical Parts ◽  
Different Parts

The ANSI Y14.5M Geometric Dimensioning and Tolerancing Standards have been used for many years by practitioners for size and form verification of manufactured parts. However, different parts may satisfy these tolerances and yet perform quite differently because of surface variations not specified in the standards. We present a statistically-based technique for the determination of the type of surface variation for cylindrical parts. This technique is based on a parametric-based part representation used for fitting. The method is illustrated with examples. [S1087-1357(00)00403-2]

Determination of the eddy turn-over time in the solar wind

2007 ◽  
Author(s):  
K. Bamert ◽  
R. Kallenbach ◽  
M. Hilchenbach ◽  
C. W. Smith
Keyword(s):  
Solar Wind ◽  
Turn Over ◽  
Over Time

Identification and measurement of testosterone in plasma and follicular fluid of the mare, using gas chromatography–mass spectrometry associated with isotope dilution

1983 ◽  
Vol 97 (1) ◽  
pp. 51-56 ◽  
Author(s):  
P. Silberzahn ◽  
L. Dehennin ◽  
I. H. Zwaïn ◽  
P. Leymarie
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Follicular Fluid ◽  
Biosynthetic Pathway ◽  
Mammalian Species ◽  
Gas Chromatography Mass Spectrometry ◽  
Plasma Testosterone ◽  
Quantitative Measurements ◽  
Chromatography Mass Spectrometry

Testosterone has been identified by mass spectrometry in blood and follicular fluid aspirated from mature Graafian follicles of mares. Quantitative measurements made by gas chromatography–mass spectrometry have validated the determination of plasma testosterone made by radioimmunoassay. However, because of high levels of epitestosterone (17α-hydroxyandrost-4-en-3-one) in the follicular fluid, radioimmunoassay overestimates the true concentrations of testosterone. The occurrence of testosterone in mare follicular fluid at a concentration which is two orders of magnitude higher than that in peripheral plasma suggests that the follicle may contribute to the production of circulating testosterone. A biosynthetic pathway for oestradiol-17β which involves testosterone is therefore likely to occur in the mare ovary as in many other mammalian species.

scholarly journals Determination of Chemical Content and some Physical Properties and Meat Pigments (Myoglobin, Meta Myoglobin and Oxymyoglobin) in Different Parts of Slaughtered Animals

2019 ◽  
Vol 32 ◽  
pp. 302-319
Author(s):  
Khadeeja S.J. Al-Husseiny ◽  
Maryam T. Khrebish
Keyword(s):  
The Body ◽  
Water Holding Capacity ◽  
Fresh Meat ◽  
Ph Values ◽  
Significant Difference ◽  
Chemical Content ◽  
Meat Samples ◽  
Water Holding ◽  
Different Parts

The current study aimed to estimate the pigments of some muscles parts taken from cows, sheep and chicken (thigh, chest and back). The chemical content including moisture, protein, lipids and ash, as well as the pH and the water holding capacity have been evaluated. Results showed that the moisture differed among three animals with high percentage of moisture, ash and lipid in back in compared with other parts of cows. while significant difference in the percentage of ash of back with other parts and in protein in chest with other parts of sheep. The significant differences were recorded in percentage of ash of three parts of chicken, also significant differences between chest and back. The water holding capacity of fresh meat samples taken from thigh, chest and back of cows, sheep and chicken significantly differ among samples. pH values which reflect a confect in water holding capacity of meat samples taken from different parts of the body and from different animal. In addition, there was a significant differences in the percentage of the presences of myoglobin, metmyoglobin and oxymyoglobin in different samples taken from different parts of the slaughtered animals.

ANALYSIS OF POSTPARTUM LACTATIONAL AMENORRHOEA IN RELATION TO BREAST-FEEDING: SOME METHODOLOGICAL AND PRACTICAL ASPECTS

2001 ◽  
Vol 33 (4) ◽  
pp. 529-549 ◽  
Author(s):  
Y. LE STRAT ◽  
J. C. THALABARD
Keyword(s):  
Breast Feeding ◽  
Statistical Modelling ◽  
Inhibitory Effect ◽  
Precise Determination ◽  
Data Sets ◽  
Reproductive Axis ◽  
Discrete Nature ◽  
Programming Skills ◽  
Over Time

A large multicentre epidemiological study was carried out by WHO between 1991 and 1995 to analyse the duration of lactational amenorrhoea in relation to breast-feeding. The main results of this analysis, which used classical statistical modelling, have been already published. However, some specific aspects of the postpartum fertility amenorrhoea and breast-feeding covariates, and more specifically the observed progressive exhaustion of the breast-feeding inhibitory effect on the reproductive axis, may justify a closer look at the validity of the statistical tools. Indeed, as has already been emphasized, analysis of large longitudinal data sets in reproduction often faces three difficulties: (i) the precise determination of the event of interest, (ii) the way to handle the time evolution of both the studied variables and their effect on the event of interest and (iii) the often discrete nature of the data and the associated problem of tied events. The first objective of the present work was to give additional insights into the estimation and quantification of the dynamics of the effect of breast-feeding over time, considering this covariate either as fixed or time-dependent. The second objective was to show how to perform the analyses using corresponding adapted procedures in widely available statistical packages, without the need for acquiring particular programming skills.

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