Effects of Different Surfactants to Petroleum Hydrocarbons Degradation of Mixed-bacteria

Author(s):  
Jia-qi Cui ◽  
Ya-qi Li ◽  
Qing-sheng He ◽  
Bing-zhi Li ◽  
Ying-jin Yuan ◽  
...  

Abstract Surfactant-enhanced remediation (SER) had been widely applied to remove petroleum hydrocarbon (PH) pollutants, but the degradation mechanism that how to affect the hydrocarbon metabolite ability of microorganism under added surfactant is raring. In this work, the combination bacteria and surfactant were selected to remove PH, and the degradation bottleneck concentration of single-bacteria was 10000.00 mg/L according to the PH degradation rate. However, the degradation efficient of mixed-bacteria was further increased in adding surfactant condition.. Among them, the PH degradation rate of Kocuria rosea + Bacillus odyssey and Microbacterium. sp + B. odyssey had been respectively reached to 81.58±6.29% (added tween-80) and 88.35±7.58% (added rhamnolipid) under above bottleneck concentration. Compared with non-added surfactant, the relative abundances of global overmaps, amino acid metabolism, and carbohydrate metabolism were increased in adding tween-80 condition. However, when added rhamnolipid, the relative abundances of ABC transporters, two-component system, and bacteria chemotaxis had been exhibited enhancement obviously, and aimed to improve of transportation, absorption and degradation of PH. Additionally, the gene abundance of alkB and nah was also significantly increased with above condition. Meanwhile, the gene abundance of alkB and nah was also significantly enhanced by above condition. Sum up, this work offers an important information to insights into the changes of mixed-bacteria function during different systems to degrade PH.

2021 ◽  
Author(s):  
Godwin James Udo ◽  
Nnanake-Abasi O. Offiong ◽  
Alfreda Nwadinigwe ◽  
Clement O. Obadimu ◽  
Aniedi E. Nyong ◽  
...  

2013 ◽  
Vol 361-363 ◽  
pp. 908-911
Author(s):  
Peng Fei Xiao ◽  
Tie Xue You ◽  
Yu Zhen Song ◽  
Shan Ying ◽  
Jian Qiao Wang

Wood-rotting fungus,Phlebia lindtneriGB 1027, was tested in toxicity assays with three surfactants in order to select surfactants for degradation assays of chlordane. Tween 80 and Triton X-100 appeared to have lower effect on the fungal growth on solid medium, while higher effect of fungal growth was observed in solid medium with SDS. Tween 80 had positive effects both on the chlordane degradation and the fungal growth. When fungus was incubated on PDB liquid medium with Tween 80 of 10 CMC after 20 d, 78.6% of chlordane was removed. In the treatments with Triton X-100, this strain showed comparatively greatest degradation rate (70.8%) of chlordane at a concentration of 2 CMC. However, when Triton X-100 concentration was higher than 2 CMC (5 and 10 CMC), the enhancement for the biodegradation of chlordane decreased.


2012 ◽  
Author(s):  
Mohd Ariffin Abu Hassan ◽  
Dionissios Mantzavinos ◽  
Ian S. Metcalfe

Kesan frekuensi rendah (20 kHz) penyinaran ultrasonik untuk penyingkiran Linear Alkylbenzene Sulphonates (LAS) daripada larutan berair telah dikaji. Penyinaran ultrasonik terhadap tiga kepekatan berbeza LAS iaitu 500 μgmol/l, 750 μgmol/l and 1000 μgmol/l telah dijalankan. Kesemua eksperimen telah dijalankan pada suhu 30°C, pada frekuensi 20 kHz, kuasa pada 125 W dan masa eksperimen selama 120 minit tanpa pengawalan terhadap pH. Hasil kajian mendapati OH· radikal mendominasi proses pengdegradasian LAS. Kadar degradasi awal bertambah dengan bertambahnya kepekatan LAS di dalam skop kajian. Penghasilan H2O2 didapati rendah dengan proses penyinaran ultrasonik terhadap LAS pada kepekatan LAS yang tinggi. Keputusan penyinaran ultrasonik terhadap LAS dengan kehadiran Br¯ sebagai pemakan radikal membuktikan bahawa OH· radikal mendominasi pengdegradasian LAS. Kata kunci: Surfaktan, ultrasonik, sisa air, jumlah karbon organik (TOC) The effect of low frequency (20 kHz) ultrasonic irradiation on the removal of sodium Linear Alkylbenzene Sulphonates (LAS) from aqueous solutions has been investigated. Sonication of three different initial concentrations of LAS, 500 μgmol/l, 750 μgmol/l and 1000 μgmol/l, were performed. All experiments used a temperature of 30°C, frequency of 20 kHz, power of 125 W, a batch time of 120 min and the pH was left uncontrolled. It was found that the main degradation of LAS at micromolar concentrations proceeded via a reaction with OH· radicals. The initial degradation rate increased with an increase in the surfactant concentration over the whole concentration range studied. The sonolysis of LAS showed a strong inhibition of H2O2 production at a higher concentration. Sonication of LAS in the presence of Br¯ suggested that OH· radicals induced degradation pathway was the dominating sonochemical degradation mechanism. Key words: Surfactants, ultrasonic, wastewater, total organic carbon (TOC)


2009 ◽  
Vol 9 (5) ◽  
pp. 477-483 ◽  
Author(s):  
H. Tran ◽  
G. M. Evans ◽  
Y. Yan ◽  
A. V. Nguyen

Photocatalytic degradation of geosmin and 2-methylisoborneol (MIB), which are two taste and odour compounds commonly found in drinking water supply sources, was investigated using an immobilised TiO2 photoreactor. It was found that the degradation of geosmin and MIB followed similar pseudo-first-order kinetics with reaction rate constants being approximately 0.025 min−1 for typical geosmin and MIB concentrations of 250 and 500 ng/L. The normalised formal quantum efficiency was calculated to be in the range of 162–182 L/mol. Influence of additives (i.e. sodium bicarbonate and alcohols) on the degradation process was also investigated. It was found that there was a small reduction in the degradation rate constants of geosmin and MIB with increasing sodium bicarbonate concentration. At 50 mg/L sodium bicarbonate the degradation rate constants decreased by approximately 5%. Similarly, for methanol and ethanol concentrations up to 35 and 50 mg/L, respectively, these constants were found to also decrease. While addition of sodium bicarbonate and alcohols was seen to have relatively small negative effects on the photocatalytic degradation performance, the magnitude of their influence was consistent with the hypothesis that the degradation mechanism of geosmin and MIB was predominately that of attack involving HO∙ radicals.


2020 ◽  
Vol 842 ◽  
pp. 98-104
Author(s):  
Jia Li ◽  
Hui Wang ◽  
Zhong Han Li ◽  
Ting Ting Zhao ◽  
Tian Tian Wang ◽  
...  

Thermal degradation of the composite constituted by high density polyethylene (HDPE) and microencapsulated red phosphorus (MRP) were studied using thermogravimetric (TG) data obtained at different heating rates. The kinetic models and parameters of the thermal degradation of MRP/HDPE composite were evaluated by FWO, KAS and IKP method. It indicates that the activation energy E of 4 % MRP/HDPE composite is higher than HDPE for three methods. MRP could improve the thermal stability and slow down the thermal degradation of HDPE. With adding MRP, the degradation mechanism of HDPE is changed and the degradation rate decreases.


2011 ◽  
Vol 414 ◽  
pp. 88-92
Author(s):  
Xiao Nan Sun ◽  
An Ping Liu ◽  
Wen Ting Sun ◽  
Shu Chang Jin

Petroleum contamination has become one of the major soil contaminations. Aiming at petroleum hydrocarbon contamination, the multi-group opposite experiments is set; this paper use some petroleum hydrocarbon-decomposing bacteria to remedy the soil contaminated by different carbon chain petroleum hydrocarbons. Compare and study the remedial results, and study the growth of the bacteria in the decomposing process. The Study shows that the degradation rate of the bacteria to short-chain petroleum hydrocarbons is relatively high; Within 40 days without nutrient substance, degradation rate of bacteria to gasoline and diesel is 80%, degradation rate of bacteria to aromatics and lubricants is 50%, the trend of bacteria’s growth curve and the degradation rate curve of each component are approximate.


Author(s):  
Yiling Zhang ◽  
Dehong Kong ◽  
Sheng Sun ◽  
Zhiguo Luo ◽  
Peng Luo

Bioremediation of soil contaminated by pipeline spills in the Daquin region of China is presented in this paper. The objective of the study was to determine the nutrient ratio required for the bioremediation of soil contaminated by Daqing crude oil. Several experiments were conducted using different proportions of C:N:P:K to choose the optimum ratio of nutrients to be applied for bioremediation, C is supplied from the crude oil in the contaminated soil. The moisture content, porosity, PH and temperature of the contaminated soil were adjusted for optimum conditions. The experimental bioremediation technology was conducted in a treatment unit that was 0.5 m in length, 0.7 m in width, and 1.5 m in height. The results showed that the degradation rate of Total Petroleum Hydrocarbons (TPH) increased with increased concentrations of crude oil in the soil. At the beginning of the bioremediation experiments, the degradation rate of THP is high, but it gradually slowed over the course of the experiment. The degradation rate of TPH averaged 98% over 8 months.


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