The Best Commercial Chemical Disinfectant for Swimming Pools Environments Contaminated With Fungi

2020 ◽  
Author(s):  
Parisa Badiee ◽  
Sara Rouein ◽  
Foroozandeh Zaravar ◽  
Fatemeh Ghasemi

Abstract Background: Swimming pools are contaminated with different microorganisms. This study aimed to investigate the fungal contamination of the swimming pools and detect the susceptibility of the isolated fungi to routinely used disinfectants.Methods: Surface and water samples were collected from different parts of 13 indoor swimming pools. Isolated fungal species were identified based on respective colony morphology, microscopic examination, and RFLP-PCR. Susceptibilities of fungal species to common disinfectants used in swimming pools were investigated, according to M38-A and M27A3 protocols.Results: Of the 795 collected specimens, 2211 isolates from 35 fungal species were identified. The most isolated fungi were filamentous hyaline hyphomycetes (especially Aspergillus spp.), and dematiaceous (especially Cladosporium spp.), followed by Mucorales, Candida species and dermatophyte species. The most contaminated places were shoes changing and dressing rooms. Sodium hypochlorite (bleach) and chloroxylenol (Dettol) were found to be effective against all isolated fungi after 2.5 and 5 minutes, respectively. Isopropyl alcohol (Afrooz disinfectant solution) was not an effect on Aspergillus spp., dematiaceous fungi, and Candida spp. after 10 minutes’ exposure. The efficacy of hydrochloric acid (liqueur de Javel) showed the most fungicidal activity against dermatophytes at all times. Conclusions: For efficient cleaning, the disinfectant must remain on the surfaces for an appropriate period. Sodium hypochlorite was efficient antifungal activity in a shorter time for killing all species of fungi. Given the different sensitivity and resistance profile of fungi to disinfectants, regular assessment of the disinfectants used for cleaning the pools is suggested.

Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde


2003 ◽  
Vol 117 (10) ◽  
pp. 811-814 ◽  
Author(s):  
Darlene E. Lubbe ◽  
Johannes J. Fagan

This random survey was to determine the flexible nasopharyngoscope disinfection practice employed by South African otolaryngologists and to establish whether a breach in the disinfection process exists. The study also aimed to identify organisms most likely to be transmitted via endoscopy and to propose a protocol for the disinfection of the flexible nasopharyngoscope.A questionnaire regarding disinfection techniques used for the flexible nasopharyngoscope was sent to 90 otolaryngologists in South Africa. All provinces were equally represented in the survey.Forty-five otolaryngologists out of a total of 90 participated in the study. Many of the otolaryngologists had no access to a flexible nasopharyngoscope and were therefore not included in the study. Fewer than 50 per cent of the 45 surgeons washed the instrument with soap/detergent and water after use. Only 42 per cent of surgeons used a FDA-approved disinfectant, 52 per cent of which immersed the scope for a shorter period than the recommended contact time. Of the 58 per cent using non-FDA-approved products, 33 per cent used only a 70 per cent Isopropyl alcohol wipe, without immersion of the scope in disinfectant solution. The remaining 25 per cent used non-FDA-approved disinfectants either by wiping or limited immersion of the scope. Of the 45 surgeons, 49 per cent used a different method of disinfection for high-risk patients.Strict guidelines have been proposed for the disinfection of this semi-critical device by the Association of Professionals for Infection Control (APIC) and the Centers for Disease Control (CDC). These guidelines are currently not being followed by many South African otolaryngologists. There is therefore a real risk of transmitting infectious diseases, especially tuberculosis, via endoscopy.


2021 ◽  
Vol 11 ◽  
Author(s):  
Yunge Liu ◽  
Lina Wu ◽  
Jina Han ◽  
Pengcheng Dong ◽  
Xin Luo ◽  
...  

The aim of this study was to assess the efficacy of four natural antimicrobial compounds (cinnamaldehyde, eugenol, resveratrol and thymoquinone) plus a control chemical disinfectant (sodium hypochlorite) in inhibiting biofilm formation by Listeria monocytogenes CMCC54004 (Lm 54004) at a minimum inhibitory concentration (MIC) and sub-MICs. Crystal violet staining assay and microscopic examination were employed to investigate anti-biofilm effects of the evaluated compounds, and a real-time PCR assay was used to investigate the expression of critical genes by Lm 54004 biofilm. The results showed that five antimicrobial compounds inhibited Lm 54004 biofilm formation in a dose dependent way. Specifically, cinnamaldehyde and resveratrol showed better anti-biofilm effects at 1/4 × MIC, while sodium hypochlorite exhibited the lowest inhibitory rates. A swimming assay confirmed that natural compounds at sub-MICs suppressed Lm 54004 motility to a low degree. Supporting these findings, expression analysis showed that all four natural compounds at 1/4 × MIC significantly down-regulated quorum sensing genes (agrA, agrC, and agrD) rather than suppressing the motility- and flagella-associated genes (degU, motB, and flaA). This study revealed that sub-MICs of natural antimicrobial compounds reduced biofilm formation by suppressing the quorum sensing system rather than by inhibiting flagella formation.


2014 ◽  
Vol 11 (3) ◽  
pp. 1122-1129
Author(s):  
Baghdad Science Journal

The present study was included the isolation of Keratinophilic fungi from water samples taken from 19 indoor public swimming pools in Baghdad during four season (summer ,fall ,spring ,and winter). Isolation period was September 2011and September 2012 . Samples were collected from different places in swimming pool . Sixteen keratinophilic fungal species, belonging to twenty –one genera were isolated using Surface Dilution Plating (SDP) and Hair Bait Technique (HBT) . Aspergillus genus was the most frequent and occurrence (19.84%), followed by Trichophyton (11.60%) and Fusarium (7.59% ) . The most common fungal infection occurred in summer season (42.16%) , and the most frequent month manifestation of species occurred in August (17%) .


2021 ◽  
Vol 6 (1) ◽  
pp. 168-172
Author(s):  
K. V. Solomakha ◽  
◽  
S. I. Harkavyi

This article is about the importance of sanitary and hygienic examination of water complexes, in particular, swimming pools and water parks. There is an increasing demand for visiting such water complexes in Ukraine, both for sports activities and for leisure activities. The focus of this article is on the sanitary and hygienic examination and survey of pool water, which is treated with sodium hypochlorite (obtained by chemical way). Material and methods. The studies were carried out for 10 weeks, including a series of water samples, which were taken during the quarantine period, when swimmers were prohibited from visiting the pool. This situation made possible to make a comparative hygienic assessment of the effect of workload on the water condition in the pool and the effectiveness of disinfection. There was also a short review and characteristics of hypochlorite A, which was obtained in chemical way, its advantages and disadvantages as a disinfectant. The article analyzes the data obtained during the sanitary and hygienic survey of the pool of the swimming pool of the national technical university, their statistical processing, and comparison with the current regulatory documents of Ukraine and some other states. The data obtained in the course of a series of studies indicated a significant human influence on the state of water in the pool, which once again indicates the importance of health education of the population and visitors in swimming pools and water parks, in particular, the need to take a shower before swimming, after using the toilet, etc.; the need for training the right culture of visiting different water objects. Particular attention should be paid to swimming pools frequented by children, as they often do not have sufficient hygiene skills, or due to age cannot constantly monitor urination, so, as a rule, it`s an acute issue of large amounts of chloramines in swimming pools for children. Conclusion. In particular, a statistically significant difference was obtained in terms of ammonia and ammonium ions (in total), which indicated a significant influence of visitors on this parameter. And, as you know, organic impurities (sweat, urine, cosmetics remains etc.) that get into the water together with the swimmers, react with chlorine and form chloramines, which can negatively affect the health of both the visitors and the staff. This must be taken into account while changing the workload on the pool and choosing the dose of disinfectant


2016 ◽  
Vol 4 (2) ◽  
pp. 132-140
Author(s):  
Rezy Kurnia ◽  
Hidayati Hidayati

Disinfection is needed to avoid transmission of infectious agents. One of disinfectant solution used is sodium hypochlorite. Disinfection should be done without changing the quality of gypsum models, so the technique of mixing a disinfectant solution on gypsum powder can be used as an alternative. The purpose of this study was to determined the effect of mixing sodium hypochlorite to the compressive strength of type III gypsum product. The study was conducted at the Laboratory of Materials and Structures, Faculty of Engineering, Andalas University using laboratory experimental method with 30 pieces of type III gypsum models as samples. Samples were divided into 5 groups, that were mixed using 0.5% sodium hypochlorite, 1% sodium hypochlorite, 2% sodium hypochlorite, 5% sodium hypochlorite and distilled water as a control group. Measurement of the compressive strength was performed with a Universal Testing Machine (UTM). The results showed that the models were mixed with 0.5% sodium hypochlorite, 1% sodium hypochlorite, 2% sodium hypochlorite and 5% sodium hypochlorite reduced the average of the compressive strength compared to the models that are mixed with distilled water. One Way ANOVA test showed that was significantly differences (p<0.05) among groups of 0.5% sodium hypochlorite, 1% sodium hypochlorite, 2% sodium hypochlorite, 5% sodium hypochlorite and distilled water as a control group. It was concluded that there were effect of mixing sodium hypochlorite to reduced the compressive strength of type III gypsum product. Keywords: type III gypsum, compressive strength, sodium hypochlorite


2015 ◽  
Vol 3 (2) ◽  
pp. 59-67
Author(s):  
Siti Coryniken ◽  
Didin Kustantiningtyastuti ◽  
Afriwardi Afriwardi

materials have character that absorb water it is imbibition that can affect the dimensional stability when immersed in disinfectant . The purpose of this study is to see the effect of imbibition at alginate impression material immersed in a disinfectant solution of Sodium Hypochlorite .The method used was experimental with pretest and posttest with control group design . The mold were diameter of 28 mm and height 18 mm .the impression were immersed in sodium hypochlorite 0.5 % and 1 % for 3 minutes , 5 minutes and 10 minutes . Imbibition weight calculation is done by using digital scales.The results showed that the presence of imbibition on impression material alginate that immersed in Sodium Hypochlorite solution for 3 minutes , 5 minutes and 10 minutes . Statistical Test Two Way Repeated Measure ANOVA showed a significant difference ( p< 0.05 ) in minutes imbibition , while the concentration of the solution had a significant difference ( p > 0.05 ) .The conclusion of this study is the alginate impression material has the effect of imbibition to immersion Sodium Hypochlorite solution. Keywords : hydrocolloid Alginate , Sodium Hypochlorite , imbibition effects , immersio


2017 ◽  
Vol 5 (4) ◽  
pp. 160-169
Author(s):  
Onuorah Samuel ◽  
Ginika-Osuorji Joy ◽  
Odibo Frederick ◽  
Ojiagu Nnenna Chinelo

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