scholarly journals Inhibition of Biofilm Formation and Related Gene Expression of Listeria monocytogenes in Response to Four Natural Antimicrobial Compounds and Sodium Hypochlorite

2021 ◽  
Vol 11 ◽  
Author(s):  
Yunge Liu ◽  
Lina Wu ◽  
Jina Han ◽  
Pengcheng Dong ◽  
Xin Luo ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Quorum Sensing ◽  
Sodium Hypochlorite ◽  
Biofilm Formation ◽  
Natural Compounds ◽  
Antimicrobial Compounds ◽  
Natural Antimicrobial ◽  
Low Degree ◽  
Chemical Disinfectant ◽  
Dose Dependent

The aim of this study was to assess the efficacy of four natural antimicrobial compounds (cinnamaldehyde, eugenol, resveratrol and thymoquinone) plus a control chemical disinfectant (sodium hypochlorite) in inhibiting biofilm formation by Listeria monocytogenes CMCC54004 (Lm 54004) at a minimum inhibitory concentration (MIC) and sub-MICs. Crystal violet staining assay and microscopic examination were employed to investigate anti-biofilm effects of the evaluated compounds, and a real-time PCR assay was used to investigate the expression of critical genes by Lm 54004 biofilm. The results showed that five antimicrobial compounds inhibited Lm 54004 biofilm formation in a dose dependent way. Specifically, cinnamaldehyde and resveratrol showed better anti-biofilm effects at 1/4 × MIC, while sodium hypochlorite exhibited the lowest inhibitory rates. A swimming assay confirmed that natural compounds at sub-MICs suppressed Lm 54004 motility to a low degree. Supporting these findings, expression analysis showed that all four natural compounds at 1/4 × MIC significantly down-regulated quorum sensing genes (agrA, agrC, and agrD) rather than suppressing the motility- and flagella-associated genes (degU, motB, and flaA). This study revealed that sub-MICs of natural antimicrobial compounds reduced biofilm formation by suppressing the quorum sensing system rather than by inhibiting flagella formation.

Quorum Sensing – A Stratagem for Conquering Multi-Drug Resistant Pathogens

2020 ◽  
Vol 26 ◽  
Author(s):  
Madison Tonkin ◽  
Shama Khan ◽  
Mohmmad Younus Wani ◽  
Aijaz Ahmad
Keyword(s):  
Drug Resistance ◽  
Quorum Sensing ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Cell Communication ◽  
Natural Compounds ◽  
Quorum Sensing Inhibitors ◽  
Communication Technique ◽  
Multicellular Organisms ◽  
Chemical Strategies

: Quorum sensing is defined as cell to cell communication between microorganisms, which enables microorganisms to behave as multicellular organisms. Quorum sensing enables many collaborative benefits such as synchronisation of virulence factors and biofilm formation. Both quorum sensing as well as biofilm formation encourage the development of drug resistance in microorganisms. Biofilm formation and quorum sensing are causally linked to each other and play role in the pathogenesis of microorganisms. With the increasing drug resistance against the available antibiotics and antifungal medications, scientists are combining different options to develop new strategies. Such strategies rely on the inhibition of the communication and virulence factors rather than on killing or inhibiting the growth of the microorganisms. This review encompasses the communication technique used by microorganisms, how microorganism resistance is linked to quorum sensing and various chemical strategies to combat quorum sensing and thereby drug resistance. Several compounds have been identified as quorum sensing inhibitors and are known to be effective in reducing resistance as they do not kill the pathogens but rather disrupt their communication. Natural compounds have been identified as anti-quorum sensing agents. However, natural compounds present several related disadvantages. Therefore, the need for the development of synthetic or semi-synthetic compounds has arisen. This review argues that anti-quorum sensing compounds are effective in disrupting quorum sensing and could therefore be effective in reducing microorganism drug resistance.

Quorum Sensing Signalling and Biofilm Formation of Brewery-Derived Bacteria, and Inhibition of Signalling by Natural Compounds

2014 ◽  
Vol 69 (5) ◽  
pp. 617-627 ◽  
Author(s):  
O. Priha ◽  
V. Virkajärvi ◽  
R. Juvonen ◽  
R. Puupponen-Pimiä ◽  
L. Nohynek ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Natural Compounds

scholarly journals Efficacy of Synthetic Furanones on Listeria monocytogenes Biofilm Formation

Foods ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 647 ◽  
Author(s):  
Pedro Rodríguez-López ◽  
Andrea Emparanza Barrenengoa ◽  
Sergio Pascual-Sáez ◽  
Marta López Cabo
Keyword(s):  
Listeria Monocytogenes ◽  
Biofilm Formation ◽  
Epifluorescence Microscopy ◽  
Dependent Manner ◽  
Antifouling Activity ◽  
Aisi 316 ◽  
Novel Strategy ◽  
And Control ◽  
Acylated Homoserine Lactones ◽  
Dose Dependent

Furanones are analogues of acylated homoserine lactones with proven antifouling activity in both Gram-positive and Gram-negative bacteria though the interference of various quorum sensing pathways. In an attempt to find new strategies to prevent and control Listeria monocytogenes biofilm formation on stainless steel (SS) surfaces, different concentrations of six synthetic furanones were applied on biofilms formed by strains isolated from food, environmental, and clinical sources grown onto AISI 316 SS coupons. Among the furanones tested, (Z-)-4-Bromo-5-(bromomethylene)-2(5H)-furanone and 3,4-Dichloro-2(5H)-furanone significantly (p < 0.05) reduced the adhesion capacity (>1 log CFU cm−2) in 24 h treated biofilms. Moreover, individually conducted experiments demonstrated that (Z-)-4-Bromo-5-(bromomethylene)-2(5H)-furanone was able to not only significantly (p < 0.05) prevent L. monocytogenes adhesion but also to reduce the growth rate of planktonic cells up to 48 h in a dose-dependent manner. LIVE/DEAD staining followed by epifluorescence microscopy visualisation confirmed these results show an alteration of the structure of the biofilm in furanone-treated samples. Additionally, it was demonstrated that 20 µmol L−1 of 3,4-Dichloro-2(5H)-furanone dosed at 0, 24 and 96 h was able to maintain a lower level of adhered cells (>1 log CFU cm−2; p < 0.05). Since furanones do not pose a selective pressure on bacteria, these results represent an appealing novel strategy for the prevention of L. monocytogenes biofilm grown onto SS.

scholarly journals Rearrangement of Thiodepsipeptides by S–N Acyl Shift Delivers Homodetic Autoinducing Peptides

2021 ◽  
Author(s):  
Bengt H. Gless ◽  
Benjamin Svejdal Bejder ◽  
Martin S. Bojer ◽  
Hanne Ingmer ◽  
Christian Adam Olsen
Keyword(s):  
Listeria Monocytogenes ◽  
Quorum Sensing ◽  
Clostridium Perfringens ◽  
Biofilm Formation ◽  
Group Behavior ◽  
Peptide Ligands ◽  
Gram Positive Bacteria ◽  
Chemically Induced ◽  
Modified Peptides ◽  
Autoinducing Peptides

Group behavior in many bacteria relies on chemically induced communication called quorum sensing (QS), which plays important roles in regulation of colonization, biofilm formation, and virulence. In Gram-positive bacteria, QS is often mediated by cyclic ribosomally synthesized and posttranslationally modified peptides (RiPPs). In staphylococci for example, most of these so-called autoinducing peptides (AIPs) contain a conserved thiolactone functionality, which has been predicted to constitute a structural feature of AIPs from other species as well. Here, we show that pentameric AIPs from <i>Lactobacillus plantarum, Clostridium perfringens, </i>and<i> Listeria monocytogenes </i>that were previously presumed to be thiolactone-containing structures readily rearrange to become homodetic cyclopeptides. This finding has implications for the developing understanding of the cross-species communication of bacteria and may help guide the discovery of peptide ligands to perturb their function.

scholarly journals Tackling Antibiotic Resistance with Compounds of Natural Origin: A Comprehensive Review

Biomedicines ◽  
2020 ◽  
Vol 8 (10) ◽  
pp. 405 ◽  
Author(s):  
Francisco Javier Álvarez-Martínez ◽  
Enrique Barrajón-Catalán ◽  
Vicente Micol
Keyword(s):  
Antimicrobial Agents ◽  
New Technologies ◽  
Protein Biosynthesis ◽  
Natural Compounds ◽  
Network Pharmacology ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Antimicrobial Compounds ◽  
Natural Antimicrobial ◽  
Drug Resistant Bacteria

Drug-resistant bacteria pose a serious threat to human health worldwide. Current antibiotics are losing efficacy and new antimicrobial agents are urgently needed. Living organisms are an invaluable source of antimicrobial compounds. The antimicrobial activity of the most representative natural products of animal, bacterial, fungal and plant origin are reviewed in this paper. Their activity against drug-resistant bacteria, their mechanisms of action, the possible development of resistance against them, their role in current medicine and their future perspectives are discussed. Electronic databases such as PubMed, Scopus and ScienceDirect were used to search scientific contributions until September 2020, using relevant keywords. Natural compounds of heterogeneous origins have been shown to possess antimicrobial capabilities, including against antibiotic-resistant bacteria. The most commonly found mechanisms of antimicrobial action are related to protein biosynthesis and alteration of cell walls and membranes. Various natural compounds, especially phytochemicals, have shown synergistic capacity with antibiotics. There is little literature on the development of specific resistance mechanisms against natural antimicrobial compounds. New technologies such as -omics, network pharmacology and informatics have the potential to identify and characterize new natural antimicrobial compounds in the future. This knowledge may be useful for the development of future therapeutic strategies.

scholarly journals Genistein Inhibits the Pathogenesis of Aeromonas hydrophila by Disrupting Quorum Sensing Mediated Biofilm Formation and Aerolysin Production

2021 ◽  
Vol 12 ◽  
Author(s):  
Jing Dong ◽  
Defu Zhang ◽  
Jianrong Li ◽  
Yongtao Liu ◽  
Shun Zhou ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Aeromonas Hydrophila ◽  
Biofilm Formation ◽  
Cell Injury ◽  
Foodborne Pathogen ◽  
Opportunistic Pathogen ◽  
Dependent Manner ◽  
Novel Drugs ◽  
Encoding Gene ◽  
Dose Dependent

Aeromonas hydrophila is an opportunistic pathogen that is responsible for a variety of infectious diseases both in human and animals, particularly aquatic animals. Moreover, the pathogen has become a foodborne pathogen by transmitting from seafood to human. The abuse of antibiotics in aquaculture results in the emergence of antibiotic resistance and treatment failure. Therefore, novel approaches are urgently needed for managing resistant A. hydrophila associated infections. Aerolysin, an essential virulence factor of pathogenic A. hydrophila strain, has been identified as target developing novel drugs against pathogenesis of A. hydrophila. In the present study, genistein, without anti-A. hydrophila activity, was identified that could decrease the production of aerolysin and biofilm formation at a dose-dependent manner. Transcription of aerolysin encoding gene aerA and quorum sensing related genes ahyI and ahyR was significantly down-regulated when co-cultured with genistein. Cell viability studies demonstrated that genistein could significantly improve aerolysin mediated A549 cell injury. Furthermore, genistein could provide a remarkable protection to channel catfish infected with A. hydrophila. These findings indicate that targeting quorum sensing and virulence can be a useful approach developing drugs against A. hydrophila infections in aquaculture. Moreover, genistein can be chosen as a promising candidate in developing drugs against A. hydrophila.

scholarly journals ANTI-QUORUM SENSING POTENTIAL OF LIMONIA ACIDISSIMA (L.) AGAINST VIBRIO HARVEYI KUMB-VA4

2018 ◽  
Vol 11 (3) ◽  
pp. 303
Author(s):  
Srinivasan P ◽  
Rajalakshmi M ◽  
Dinesh Kumar S
Keyword(s):  
Quorum Sensing ◽  
Ethyl Acetate ◽  
Molecular Characterization ◽  
Metabolic Activity ◽  
Biofilm Formation ◽  
Vibrio Harveyi ◽  
Dependent Manner ◽  
Biofilm Inhibition ◽  
Limonia Acidissima ◽  
Dose Dependent

 Objective: This study aims to investigate the quorum-sensing inhibition (QSI) potential of Limonia acidissima L. against the biofilm forming Vibrio harveyi isolated from freshwater fish.Methods: The present study evaluated the anti-QS activity of the L. acidissima methanol and ethyl acetate (LA-M and LA-EA) fruit extracts using Chromobacterium violaceum ATCC 12472 (wild) and C. violaceum CV026 (mutant) as biomonitor strains and biofilm formation using the crystal violet assay. Vibrio sp. were isolated from freshwater-cultured fishes and screened for biofilm formation property. Strong biofilm forming isolate were subjected to molecular characterization. Limonia fruit pulp was subjected to methanol and ethyl acetate extraction using cold percolation method and yield was calculated. In parallel to determining the QSI properties of the extract, minimum inhibitory concentration (MIC), biofilm inhibition concentration (BIC), antibiofilm properties, and metabolic activity of LA-M and LA-EA against the biofilm forming V. harveyi KUMB-VA4 was determined.Results: The results of the present study demonstrated that the overall yield of methanol and ethyl acetate extract was 12.84% and 9.3% (w/w), respectively. Strong biofilm forming Vibrio isolate KUMB-VA4 was obtained from infected freshwater fishes and was subjected to molecular characterization. MIC of LA-M was 1510 μg/ml and LA-EA was observed to be 3000 μg/ml against the test pathogen, respectively. Biofilm inhibition assay revealed a BIC of LA-M at 250 μg/ml and LA-EA at 500 μg/ml. Both the plant extracts significantly reduced the biofilm formation of V. harveyi KUMB-VA4 and the metabolic activity in a dose-dependent manner. Light microscopy and scanning electron microscopy revealed that LA-M and LA-EA significantly altered 68.6% and 54.5% of the biofilm architecture at BIC. The QSI assay revealed that LA-M effectively reduced the violacein production of the biomonitor strains at sub-BIC (100–500 μg/ml) to 80% than LA-EA (43%) in a strong dose-dependent fashion.Conclusions: The present study revealed the QSI property of Limonia acidissima against the biofilm forming V. harveyi isolated from infected fish.

scholarly journals Rearrangement of Thiodepsipeptides by S–N Acyl Shift Delivers Homodetic Autoinducing Peptides

2021 ◽  
Author(s):  
Bengt H. Gless ◽  
Benjamin Svejdal Bejder ◽  
Martin S. Bojer ◽  
Hanne Ingmer ◽  
Christian Adam Olsen
Keyword(s):  
Listeria Monocytogenes ◽  
Quorum Sensing ◽  
Clostridium Perfringens ◽  
Biofilm Formation ◽  
Group Behavior ◽  
Peptide Ligands ◽  
Gram Positive Bacteria ◽  
Chemically Induced ◽  
Modified Peptides ◽  
Autoinducing Peptides

Group behavior in many bacteria relies on chemically induced communication called quorum sensing (QS), which plays important roles in regulation of colonization, biofilm formation, and virulence. In Gram-positive bacteria, QS is often mediated by cyclic ribosomally synthesized and posttranslationally modified peptides (RiPPs). In staphylococci for example, most of these so-called autoinducing peptides (AIPs) contain a conserved thiolactone functionality, which has been predicted to constitute a structural feature of AIPs from other species as well. Here, we show that pentameric AIPs from <i>Lactobacillus plantarum, Clostridium perfringens, </i>and<i> Listeria monocytogenes </i>that were previously presumed to be thiolactone-containing structures readily rearrange to become homodetic cyclopeptides. This finding has implications for the developing understanding of the cross-species communication of bacteria and may help guide the discovery of peptide ligands to perturb their function.

scholarly journals Effect of different conditions on Listeria monocytogenes biofilm formation and removal

2018 ◽  
Vol 36 (No. 3) ◽  
pp. 208-214 ◽  
Author(s):  
Pasquale Russo ◽  
Agni Hadjilouka ◽  
Luciano Beneduce ◽  
Vittorio Capozzi ◽  
Spiros Paramithiotis ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Listeria Monocytogenes ◽  
Sodium Hypochlorite ◽  
Biofilm Formation ◽  
Benzalkonium Chloride ◽  
Food Products ◽  
Growth Media ◽  
Hydrophilic Surfaces ◽  
Different Temperatures ◽  
Process Line

Listeria monocytogenes poses a major risk for the safety of food products due to the ability to persist in food products and process line surfaces as biofilm. In this work, we investigated the L. monocytogenes biofilms in relation to development factors and possible control under different conditions. In particular, the ability of six strains of L. monocytogenes from vegetable and animal sources to form biofilms was evaluated on glass or polystyrene substrates under different temperatures (15, 30 and 37°C) and availability of nutrients, by using rich (BHI) or poor (HTM) growth media. Moreover, the effectiveness of three commonly used sanitizers (benzalkonium chloride, sodium hypochlorite and hydrogen peroxide) was compared to eradicate established biofilms. Our results showed that starved conditions, hydrophilic surfaces, and high temperatures increased the L. monocytogenes ability to produce biofilms. In general, benzalkonium chloride was the most effective chemical to remove established biofilms.

Sign in / Sign up

Export Citation Format

Share Document