Association of MMP9 Polymorphism With Myocardial Infarction
Abstract Myocardial Infarction (MI) is the most common type of coronary heart disease characterized by pain, nausea, sweating, shortness of breath and abnormal heartbeat. Current study was conducted to identify the susceptibility of the MMP-9 gene among families affected with myocardial infarction in the Pakistani population. A family clustering study based on 5 families having MI patients was conducted. Blood samples from MI patients and their family members were collected for further genetic analysis. Patients were with a mean BMI of ±30.2 kg/m2 which indicates that obese were at high risk of disease development. The mean age for diagnosis of disease was ±50 years, in which all patients had a positive family history. Most patients were diabetic with hypertension, most of them were smokers and their exposure to air pollution was higher. Genomic DNA was isolated from the blood through manual extraction. Primers were optimized and genotyping was done by polymerase chain reaction (PCR) which was followed by DNA sequencing and restriction fragment length polymorphism (RFLP). As a result of polymorphism, A into G and C into T conversions were identified on rs17576 and rs3918242 polymorphic sites on the MMP-9 gene respectively. In conclusion smoking, hypertension, diabetes, exposure to air pollution and polymorphism of rs17576 and rs3918242 were significantly associated with the onset of MI in the Pakistani population and males were at higher risk. Further studies should be conducted on large scale to evaluate the association of MMP-9 polymorphism with MI.