scholarly journals Effects of slurry reflux on the stability and microbial community structure of corn stalk anaerobic digestion system

2021 ◽  
Author(s):  
Ling Zhao ◽  
Yang Gao ◽  
Jiamin Zhao ◽  
Jiaxing Sun ◽  
Zhen Wang ◽  
...  
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Buffer Capacity ◽  
High Throughput Sequencing ◽  
Organic Load ◽  
Corn Stalk ◽  
Load Rate ◽  
The Stability ◽  
Archaea Community ◽  
Organic Load Rate

Abstract Anaerobic digestion(AD) of corn stalks with slurry reflux and non-reflux was compared and evaluated,to clarify the effects of slurry reflux on AD. The reflux of slurry increased methane production by 45.8% and also improved the buffer capacity and adaptability to high organic load rate(OLR). The results of high-throughput sequencing showed that slurry reflux increased the richness of bacterial and archaea community, and decreased the diversity of the microbial community. Slurry reflux also reduced the tendency of the enrichment of Chloroflexi and the relative abundance of Methanothrix. The increase of OLR changed the main methanogenic pathway in the anaerobic digestion system, slurry reflux could slow down this trend.

Storage of biodegradable polymers by an enriched microbial community in a sequencing batch reactor operated at high organic load rate

2005 ◽  
Vol 80 (11) ◽  
pp. 1306-1318 ◽  
Author(s):  
Davide Dionisi ◽  
Mario Beccari ◽  
Simona Di Gregorio ◽  
Mauro Majone ◽  
Marco Petrangeli Papini ◽  
...  
Keyword(s):  
Microbial Community ◽  
Biodegradable Polymers ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Organic Load ◽  
Load Rate ◽  
Organic Load Rate

Effect of ethanol pre-fermentation on organic load rate and stability of semi-continuous anaerobic digestion of food waste

2020 ◽  
Vol 299 ◽  
pp. 122587 ◽  
Author(s):  
Xinxin Ma ◽  
Miao Yu ◽  
Na Song ◽  
Banghua Xu ◽  
Ming Gao ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Food Waste ◽  
Organic Load ◽  
Load Rate ◽  
Organic Load Rate

Experimental Studies on Anaerobic Digestion of Straw Stalk Mixed with Pig Dung

2014 ◽  
Vol 955-959 ◽  
pp. 2774-2778
Author(s):  
Xiao Fei Sun ◽  
Shuang Ying Zheng ◽  
Xue Tong Liu ◽  
Hui Ye
Keyword(s):  
Anaerobic Digestion ◽  
Volume Fraction ◽  
Ph Value ◽  
Experimental Studies ◽  
Ammonia Nitrogen ◽  
Gas Production ◽  
Total Alkalinity ◽  
Organic Load ◽  
Load Rate ◽  
Organic Load Rate

A series of anaerobic digestion experiments on straw stalk mixed with pig dung was carried out at mid-temperature (35°C) by using the self-designed continuous stirring tank reactor (CSTR) in different organic load rates (OLR). Anaerobic digestion indexes, such as pH, ammonia-nitrogen, volatile fatty acid (VFA), total alkalinity, quantity of gas production and production rate of methane under the different organic load were studied and the aerogenic capability of the system and its stability were analyzed, so as to find the optimal organic load rate for anaerobic digestion reaction. The results show that: (1) With the OLR increases from 0.5-2.0g VS/(L·d) step by step, the pH value is always maintained 6.7-7.2; the average daily gas-production quantity is 869-3232 mL; the average ammonia-nitrogen concentration in the anaerobic digestion process has kept 522-850mg/L; the VFA concentration has remained between 1520-3420mg/L; the average total alkalinity is among 2152-2441mg/L . (2) The anaerobic digestion system is stable when the OLR increases from 0.5-2.0g VS/(L·d), and the optimal organic load rate is 1.5g VS/(L·d) for volume fraction of methane.

scholarly journals Effects of Slurry Reflux on the Stability and Microbial Community Structure of Corn Stalk Anaerobic Digestion System

2021 ◽  
Author(s):  
ling zhao ◽  
yang gao ◽  
jiamin zhao ◽  
jiaixng sun ◽  
zhen wang ◽  
...  
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
High Throughput Sequencing ◽  
Loading Rate ◽  
Ph Value ◽  
Community Diversity ◽  
System Stability ◽  
Organic Loading Rate ◽  
Organic Loading ◽  
The Stability

Abstract Anaerobic digestion (AD) of corn stalks with slurry reflux and non-reflux was compared and evaluated，to clarify the effects of slurry reflux on AD. It was found that slurry reflux increased cumulative methane production by 45.80% and improved system stability. With the increase of organic loading rate (OLR), pH value and oxidation-reduction potential (ORP) of reflux group remained 7.16±0.23 and -338.71±9.22. High-throughput sequencing results showed that slurry reflux slowed down the decrease of microbial community diversity, and the richness of bacterial community increased by 9.16%. The dominant microorganisms were Bacteroidetes and Methanothrix in reflux group, the relative abundances were 32.41% and 41.75%, respectively. The increase of organic loading rate (OLR) altered the main methane-producing pathway of the AD system, and slurry reflux can delay this trend.

Characterization and Comparison of Biomass from Mesophilic and Thermophilic Fixed Bed Anaerobic Digesters

1992 ◽  
Vol 25 (7) ◽  
pp. 203-212 ◽  
Author(s):  
M. Soto ◽  
R. Méndez ◽  
J. M. Lema
Keyword(s):  
Kinetic Parameters ◽  
Fixed Bed ◽  
Organic Load ◽  
Biomass Distribution ◽  
Anaerobic Digesters ◽  
Long Period ◽  
Load Rate ◽  
Attached Biomass ◽  
Different Levels ◽  
Organic Load Rate

Two lab-scale mesophilic (MAF) and thermophilic (TAF) anaerobic filters treating effluents from a mussel cooking factory were operated at their maximum organic load rate (OLR) for a long period of time. The biomass profiles and the distribution of occluded and attached biomass were determined. Biomass distribution in the MAF was more homogeneous along the filter. Most of the biomass in the TAF was attached while both attached and occluded biomass was observed in the MAF. The hydrolytic, acidogenic, acetogenic and methanogenic activities of sludges at different levels were determined. An attempt is made to explain the behaviour of both reactors as a function of these activities and other kinetic parameters.

scholarly journals Removal of nutrients and organic pollutants from domestic wastewater treatment by sponge-based moving bed biofilm reactor

2019 ◽  
Vol 25 (5) ◽  
pp. 652-658
Author(s):  
Huynh Tan Nhut ◽  
Nguyen Tri Quang Hung ◽  
Tran Cong Sac ◽  
Nguyen Huynh Khanh Bang ◽  
Tran Quang Tri ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Treatment Plan ◽  
Domestic Wastewater ◽  
Biofilm Reactor ◽  
Organic Load ◽  
Moving Bed Biofilm Reactor ◽  
Moving Bed ◽  
Domestic Wastewater Treatment ◽  
Load Rate ◽  
Organic Load Rate

This study evaluates the efficiency of domestic wastewater treatment via Sponge-Based Moving Bed Biofilm Reactor (S-MBBR). The laboratory-based treatment plan uses polyurethane sponge with a specific surface area was 260 m<sup>2</sup>/m<sup>3</sup> as a carrier. The treatment plan operated under four different organic load rate: OLR1 = 0.4 kg BOD/m<sup>3</sup>.day; OLR2 = 0.6 kg BOD/m<sup>3</sup>.day; OLR3 = 0.8 kg BOD/m<sup>3</sup>.day; and OLR4 = 1.0 kg BOD/m<sup>3</sup>.day. During 80 d of the experiment, the highest treatment efficiency was at the organic load rate of 0.4 kg BOD/m<sup>3</sup>.day, with COD, SS, TN and TP were found to be 85.0 ± 12.9%, 85.7 ± 5.3%, 68.9 ± 1.7%, and 40.3 ± 0.2%, respectively. In which, the influent SS concentration were from 117.3 to 126.0 mg/L, the effluent concentration were in ranged 18.0 to 34.22 mg/L, respectively. The values of influent and effluent COD were 298.8 ± 12.88 and 44.8 ± 3.78 mg/L in turn. The OLR1 influent TN, TP concentrations were respectively 47.9 ± 2.11 and 3.6 ± 0.15 mg/L; the effluent TN, TP concentration were 14.9 ± 0.18 and 2.2 ± 0.06 mg/L, respectively. The study suggests that the effluent is within the allowable limits of National technical regulation on domestic wastewater (Column B1), indicating the applicability of S-MBBR for the domestic wastewater treatment plant.

scholarly journals Impact of Operational Parameters on Bacterial Community in a Full-Scale Municipal Wastewater Treatment Plant

2012 ◽  
Vol 61 (1) ◽  
pp. 41-49 ◽  
Author(s):  
AGNIESZKA CYDZIK-KWIATKOWSKA ◽  
MAGDALENA ZIELIŃSKA ◽  
IRENA WOJNOWSKA-BARYŁA
Keyword(s):  
Wastewater Treatment ◽  
Bacterial Community ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Organic Load ◽  
Municipal Wastewater Treatment ◽  
Load Rate ◽  
Organic Load Rate ◽  
Ammonia Oxidising Bacteria

A bacterial community in activated sludge from a full-scale municipal wastewater treatment plant was monitored throughout the year with the use of FISH, RISA and DGGE techniques. In the investigated range of temperatures (11.9-21.6 degrees C), a rise in temperature resulted in a lower total bacteria richness, while organic load rate changes from 0.09 to 0.21 g COD x g TSS(-1) x d(-1) were positively correlated with the number of bands in RISA patterns. The most diverse pattern (29 different bands) was characteristic for the activated sludge sample collected at the end of January at wastewater temperature of 11.9 degrees C. The ammonia-oxidising bacteria community did not change during the study, and comprised of 4 different bacterial populations with one dominant species closely related to Nitrosospira sp. REGAU (GenBank accession number AY635572.1). The percentage of ammonia-oxidising bacteria in the activated sludge varied from 6.2 to 19.5% and depended on temperature (R = 0.61, p = 0:02) and organic load rate (R = -0.55, p = 0.04).

scholarly journals Correlating Microbial Community Characteristics with Environmental Factors along a Two-Stage Biological Aerated Filter

Water ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3317
Author(s):  
Yuchen An ◽  
Songmin Li ◽  
Xiaoling Wang ◽  
Yuyang Liu ◽  
Ruonan Wang
Keyword(s):  
Microbial Community ◽  
Environmental Factors ◽  
High Throughput Sequencing ◽  
Pollutant Removal ◽  
Organic Load ◽  
Biological Aerated Filter ◽  
Community Characteristics ◽  
Two Stage ◽  
Degrading Bacteria ◽  
Aerated Filter

The purification effect of a biological aerated filter (BAF) mainly comes from the microorganisms in the reactor. Understanding the correlation between microbial community characteristics and environmental factors along the filter has great significance for maintaining good operation and improving the removal efficiency of the filter. A two-stage BAF was employed to treat domestic sewage under organic loads of 1.02 and 1.55 kg/m3·d for 15 days each. 16S rDNA high-throughput sequencing technology and redundancy analysis were applied to explore the correlation between microbial community characteristics and environmental variables. The results showed that: (1) the crucial organic-degrading bacteria in the A-stage filter were of the genus Novosphingobium, which had a significant increase in terms of relative abundance at sampling outlet A3 (135 cm of the filling height) after the increase of organic load; (2) the microbial communities at different positions in the B-stage filter were similarly affected by environmental factors, and the main bacteria associated with nitrogen removal in the B-stage filter were Zoogloea and Rhodocyclus; and (3) to improve the pollutant removal performance of this two-stage biological aerated filter, a strategy of adding an internal circulation in the B-stage filter can be adopted.

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