Application of Cryopreservation Technique in the Preservation of Rat Limbs

2020 ◽  
Author(s):  
Yu Tian ◽  
Nan Li ◽  
Wei Wang ◽  
Na Li
Keyword(s):  
Animal Experiments ◽  
Storage Conditions ◽  
Sprague Dawley ◽  
Uw Solution ◽  
Tissue Morphology ◽  
University Of Wisconsin ◽  
Sprague Dawley Rats ◽  
Nerve Bundles ◽  
Healthy Adult Male ◽  
The University

Abstract Objective: This study aims to observe the physiological and pathological changes of severed fingers (limbs) under different storage conditions through animal experiments, and to screen out the best preservation conditions. Medthods: Sixty healthy adult male Sprague-Dawley rats were selected and evenly divided into 4 preservation groups, including conventional low-temperature dry (CLTD), the university of wisconsin (UW) solution, cryopreservation and cryopreservation + UW solution preservation group. After harvesting the limbs, were preservated for 72 h and 7 days, respectively. Then the limbs were thawed and replanted in situ. Sciatic nerves were collected for hematoxylin and eosin (HE) staining, and observed the changes in tissue morphology. Results: Replantation was successful in 11 out of 15 rats (73%) in cryopreservation + UW group, and the walking function of the 9 (82%) rats in cryopreservation + UW group were significantly better than that of the cryopreservation preservation group. In addition, the HE staining results shown that the CLTD group nerve bundles were morphologically damaged, and there were more acellular structures and tissue fragments; the UW group nerve bundles were less injured and the perineurium was more complete and orderly; The nerve bundles in the cryopreservation group and cryopreservation + UW group are tightly arranged and the tissue morphology is regular; Compared with the cryopreservation + UW group, the complete of the cryopreservation group is not well. Conclusions: The cryopreservation technology combined with UW solution is a new and effective method for the severed limbs preserving.

R426 – Muscle-Nerve-Muscle Neurotization of the Rat Larynx

2008 ◽  
Vol 139 (2_suppl) ◽  
pp. P186-P186
Author(s):  
Bradford A Bader ◽  
Sang Su Oh ◽  
Matthew Owen Old ◽  
Joseph M Corey ◽  
Eva L Feldman ◽  
...  
Keyword(s):  
Vocal Fold ◽  
Neck Surgery ◽  
Nerve Graft ◽  
Motor Endplate ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Group A ◽  
Muscle Nerve ◽  
Nerve Muscle ◽  
The University

Problem The ability to reliably restore physiologic movement to a paralyzed vocal fold is not yet in hand. Muscle-nerve-muscle (M-N-M) neurotization is a process whereby axons sprouting from within an innervated muscle are directed toward a denervated muscle via a neural conduit. Given the paired laryngeal musculature, M-N-M neurotization holds promise for restoring physiologic vocal fold movement in cases of unilateral paralysis. This study is aimed at exploring the effectiveness of M-N-M neurotization in a rat model. Methods Twelve Sprague-Dawley rats were divided equally into Control and M-N-M groups. The left recurrent laryngeal nerve was transected in both groups, and in the M-N-M group a nerve graft was interposed between the innervated right thyroarytenoid (TA) muscle and the denervated left TA muscle via bilateral laryngeal windows. Vocal fold mobility was serially documented pre- and post-operatively by video-assisted endoscopy. The rats will be sacrificed at 6 weeks and immunohistochemistry used to quantify percent nerve-motor endplate contact in the left TA muscles. Myelinated axons per cross-section of the nerve graft will be counted. Results The initial surgical and endoscopic procedures were successful in all rats. Immunohistochemical data, endoscopic findings, and axon counts in the M-N-M grafts will be presented as measures of reinnervation, with comparisons between groups for percent nerve-motor endplate contact and endoscopy data. Conclusion M-N-M grafts can be performed in a rat laryngeal model and the degree of successful neurotization can be determined by this analysis paradigm. Significance This study contributes new data toward the eventual goal of restoring movement to the paralyzed vocal fold. Support This project is supported by the University of Michigan's Program for Neurology Research and Discovery and the Department of Otolaryngology - Head and Neck Surgery.

Alterations in rat adipose tissue morphology induced by a low-temperature environment

1982 ◽  
Vol 242 (2) ◽  
pp. E93-E96 ◽  
Author(s):  
W. H. Miller ◽  
I. M. Faust
Keyword(s):  
Adipose Tissue ◽  
Sprague Dawley ◽  
High Fat ◽  
Fat Depots ◽  
High Carbohydrate ◽  
Tissue Morphology ◽  
Age Related ◽  
Sprague Dawley Rats ◽  
Temperature Environment ◽  
Rat Adipose Tissue

Rats raised in the cold showed an unusual pattern of adipose tissue morphology. Male Sprague-Dawley rats were maintained in a 5 degrees C environment for up to 24 wk and the cellularity of their major adipose depots was determined. Normal age-related increases in adipocyte number were absent in two major fat depots (retroperitoneal and inguinal), whereas there was a supranormal increase in a third (epididymal). This pattern of hyperplasia contrasts sharply with that seen in rats fed highly palatable high-fat or high-carbohydrate diets in which retroperitoneal depots show the most hyperplasia and epididymal pads the least. Such variations of response across depots suggest that the features of adipose tissue responsible for adipocyte proliferation in the various depots may not be homogeneous both in their nature and in their distribution.

PITUITARY RESPONSE TO METOCLOPRAMIDE IN THE RAT

1977 ◽  
Vol 74 (1) ◽  
pp. 155-156 ◽  
Author(s):  
V. S. FANG ◽  
D. A. ZIMO ◽  
R. BYYNY
Keyword(s):  
New York ◽  
Anterior Pituitary ◽  
Pituitary Hormones ◽  
University Of Chicago ◽  
Sprague Dawley ◽  
Prolactin Release ◽  
Anterior Pituitary Hormones ◽  
Potent Stimulator ◽  
Sprague Dawley Rats ◽  
The University

Department of Medicine, The University of Chicago, Chicago, Illinois 60637, U.S.A. (Received 14 February 1977) Metoclopramide is a potent stimulator of prolactin release in man (Delitala, Masala, Alagna & Devilla, 1975; Judd, Lazarus & Smythe, 1976; McCallum, Sowers, Hershman & Sturdevant, 1976); its possible effect on other anterior pituitary hormones is not known. We have carried out two experiments with male Sprague-Dawley rats, 130–150 g in weight; the results presented here demonstrate that metoclopramide specifically stimulates the release of prolactin. Groups of five rats were injected s.c. with 0·9% saline (5 ml/kg body wt) containing 0-10 mg metoclopramide/kg (supplied by A. H. Robins Co., Richmond, Virginia). Thirteen minutes later the rats were immobilized with ketamine (supplied by Bristol Laboratories, Syracuse, New York), 100 mg/kg, i.p.; ketamine does not affect the level of prolactin in the plasma of rats (Lawson & Gala, 1974). Blood was drawn by heart puncture within 2

scholarly journals PENURUNNYA KECEMASAN TIKUS OVARIEKTOMI SETELAH LATIHAN INVERVAL DENGAN INTENSITAS TINGGI

2019 ◽  
Vol 9 (2) ◽  
pp. 1-8
Author(s):  
Saidah Rauf
Keyword(s):  
Open Field Test ◽  
Animal Experiments ◽  
Ovariectomized Rats ◽  
Depression And Anxiety ◽  
Sprague Dawley ◽  
Interval Exercise ◽  
Sprague Dawley Rats ◽  
High Intensity Interval ◽  
Keywords Anxiety

It is well established that a decrease of estrogen during natural or surgical menopause (ovariectomy)could influence female anxiety behaviour both in human and animal experiments. Several studies haveproved that exercise act as a potential therapy to reduce depression and anxiety behaviours. The currentstudy analyzed the effects of High-Intensity Interval Exercise (HIIE) to anxiety-related behaviours ofrats after long-term ovaries removal. Fifteen Sprague-Dawley rats (twelve weeks old) were used in thisstudy. All rats were divided into sham-operated, ovariectomy, and ovariectomy with HIIE (OVXHIIE). The HIIE group was treated for 7 weeks of interval exercise that started after twelve weeks ofovariectomized using treadmill. Anxiety related behaviour were represented by frequencies toentrances centre arena, freezing and grooming duration on open field test. A significant decrease(p<0.05) in the frequencies to enter open arena was detected in ovariectomized rats compared to shamand ovariectomy with HIIE, while reversed effect has observed in freezing duration. The present resultsshow that HIIE may help to counteract the ovariectomized effect on anxiety-related behaviours of rats.The HIIE may become the promising therapy to treat anxiety behaviour on menopause condition. Keywords: Anxiety, interval exercise, ovariectomy, menopause

scholarly journals Remote Inflammatory Preconditioning Alleviates Lipopolysaccharide-Induced Acute Lung Injury via Inhibition of Intrinsic Apoptosis in Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Yong Liu ◽  
Jiahang Xu ◽  
Liang Zhao ◽  
Jing Cheng ◽  
Baojun Chen
Keyword(s):  
Oxidative Stress ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Animal Experiments ◽  
Intrinsic Apoptosis ◽  
Sprague Dawley ◽  
Sod Activity ◽  
Lung Protection ◽  
Sprague Dawley Rats ◽  
The Right

Background. Acute lung injury (ALI) always leads to severe inflammation. As inflammation and oxidative stress are the common pathological basis of endotoxin-induced inflammatory injury and ischemic reperfusion injury (IRI), we speculate that remote ischemic preconditioning (RIPC) can be protective for ALI when used as remote inflammatory preconditioning (RInPC). Method. A total of 21 Sprague-Dawley rats were used for the animal experiments. Eighteen rats were equally and randomly divided into the control (NS injection), LPS (LPS injection), and RInPC groups. The RInPC was performed prior to the LPS injection via tourniquet blockage of blood flow to the right hind limb and adopted three cycles of 5 min tying followed by 5 min untying. Animals were sacrificed 24 hours later. There were 2 rats in the LPS group and 1 in the RInPC group who died before the end of the experiment. Supplementary experiments in the LPS and RInPC groups were conducted to ensure that 6 animals in each group reached the end of the experiment. Results. In the present study, we demonstrated that the RInPC significantly attenuated the LPS-induced ALI in rats. Apoptotic cells were reduced significantly by the RInPC, with the simultaneous improvement of apoptosis-related proteins. Reduction of MPO and MDA and increasing of SOD activity were found significantly improved by the RInPC. Increasing of TNF-α, IL-1β, and IL-6 induced by the LPS was inhibited, while IL-10 was significantly increased by RInPC, compared to the LPS group. Conclusion. RInPC could inhibit inflammation and attenuate oxidative stress, thereby reducing intrinsic apoptosis and providing lung protection in the LPS-induced ALI in rats.

scholarly journals Methylene Blue Attenuates Lung Injury Induced by Hindlimb Ischemia Reperfusion in Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Liangrong Wang ◽  
Baihui Chen ◽  
Bi Lin ◽  
Yuzhu Ye ◽  
Caiying Bao ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Lung Injury ◽  
Lung Tissue ◽  
Morphological Changes ◽  
Ischemia Reperfusion ◽  
Lavage Fluid ◽  
Sprague Dawley ◽  
Sod Activity ◽  
Sprague Dawley Rats ◽  
Healthy Adult Male

Objective. This study was aimed to investigate the protective effect of methylene blue against lung injury induced by reperfusion of ischemic hindlimb in a rat model. Methods. Twenty-four healthy adult male Sprague-Dawley rats were equally randomized into three groups: sham (SM) group, ischemia reperfusion (IR) group, and methylene blue (MB) group. Rats in both IR and MB groups were subjected to 4 h of ischemia by clamping the left femoral artery and then followed by 4 h of reperfusion. Treatment with 1% methylene blue (50 mg/kg) was administrated intraperitoneally at 10 min prior to reperfusion in the MB group. After 4 h of reperfusion, malondialdehyde (MDA) level, myeloperoxidase (MPO), and superoxide dismutase (SOD) activities in lung tissue were detected; inflammatory cytokines, including IL-1β and IL-6, were measured in bronchoalveolar lavage fluid (BALF); correspondingly, the morphological changes and water content in both gastrocnemius muscle and lung samples were evaluated. Results. Hindlimb IR caused remarkable morphological abnormalities and edema in both muscle and lung tissues. SOD activity was decreased, both the MPO activity and MDA level in lung tissue, as well as IL-1β and IL-6 levels in BALF, were increased in the IR group (p<0.05). Compared with the IR group, SOD activity was increased, whereas MPO activity and MDA level in lung tissue and IL-1β and IL-6 levels in BALF were decreased in the MB group (p<0.05). Also, the histological damage and edema in both lung and muscle tissues were significantly attenuated by the treatment of methylene blue. Conclusion. Methylene blue attenuates lung injury induced by hindlimb IR in rats, at least in part, by inhibiting oxidative stress.

scholarly journals Pre-Clinical Pharmacokinetic Characterization, Tissue Distribution, and Excretion Studies of Novel Edaravone Oral Prodrug, TEJ-1704

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1406
Author(s):  
Dong Wook Kang ◽  
Ju Hee Kim ◽  
Kyung Min Kim ◽  
Seok-jin Cho ◽  
Hee-Woon Jang ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Tissue Distribution ◽  
Animal Experiments ◽  
Oral Formulation ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Beagle Dogs ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Oral Prodrug

Edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one) is a free radical scavenger approved for the treatment of amyotrophic lateral sclerosis, a fatal neuromuscular disease. Edaravone is administered as an intravenous infusion over 60 min for several treatment cycles. To ease the burden of patients and caregivers, the oral formulation of edaravone has been developed. The purpose of this study was to evaluate pharmacokinetics and tissue distribution of TEJ-1704, an edaravone oral prodrug, in male Sprague Dawley rats and beagle dogs. Animal experiments were conducted using Sprague Dawley rats and beagle dogs to evaluate pharmacokinetics, tissue distribution, and excretion of TEJ-1704. Blood, tissues, cerebrospinal fluid, urine, and feces samples were collected at designated sampling time after intravenous (IV) or oral (PO) administration of edaravone or TEJ-1704. A modified bioanalysis method was developed to quantify edaravone in samples including plasma, tissues, cerebrospinal fluid, urine, and feces. The bioanalysis method was validated and successfully applied to pharmacokinetics, tissue distribution, and excretion studies of the novel edaravone prodrug. Although plasma Cmax of TEJ-1704 was low, groups administered with TEJ-1704 had high AUCinf, suggesting continuous metabolism of TEJ-1704 into edaravone. Groups treated with TEJ-1704 also showed lower CSF distribution than the control groups. After the administration of TEJ-1704, the majority of edaravone was distributed to the heart, lung, and kidney. It was excreted equally via urine and feces. The pharmacokinetics, tissue distribution, and excretion of TEJ-1704, a novel edaravone oral prodrug, were successfully characterized. Additional studies are needed to fully understand the difference between TEJ-1704 and edaravone and determine the potency of TEJ-1704.

scholarly journals A Comparison of the Preservation of Mouse Adipose Tissue-Derived Mesenchymal Stem Cells Using the University of Wisconsin Solution and Hank’s Balanced Salt Solution

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Saifun Nahar ◽  
Yoshiki Nakashima ◽  
Chika Miyagi-Shiohira ◽  
Takao Kinjo ◽  
Naoya Kobayashi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cell Viability ◽  
Salt Solution ◽  
Tissue Mass ◽  
Uw Solution ◽  
University Of Wisconsin ◽  
Preservation Solutions ◽  
The University

Preservation of adipose tissue before the isolation of cells is one of the most important steps in maintaining the cell viability of adipose tissue-derived mesenchymal stem cells (ADSCs) for clinical use. Hank’s balanced salt solution (HBSS) is one of the main ADSC preservation solutions used clinically. However, this step is known to lead to decreased cell viability. The University of Wisconsin (UW) solution is recognized by transplant physicians as an excellent organ preservation solution. We aimed to investigate the effectiveness of UW solution in preservation of the viability of ADSCs. We collected adipose tissue from the inguinal fat pad of mice and compared preservation in UW solution and HBSS overnight by measuring cell viability after isolation. We found that the number of viable cells harvested per gram of adipose tissue mass was higher in UW solution- than HBSS-preserved tissue.

Ultrastructural Investigation of Spontaneous Pituitary Adenomas in Aging Sprague-Dawley Rats

1982 ◽  
Vol 40 ◽  
pp. 216-217
Author(s):  
D. J. McComb ◽  
J. Beri ◽  
F. Zak ◽  
K. Kovacs
Keyword(s):  
Microscopic Study ◽  
Pituitary Adenomas ◽  
Wistar Rats ◽  
Microscopic Level ◽  
Sprague Dawley ◽  
Prolactin Cells ◽  
Light Microscopic Level ◽  
Ultrastructural Investigation ◽  
Sprague Dawley Rats ◽  
Long Evans

Investigation of the spontaneous pituitary adenomas in rat have been limited mainly to light microscopic study. Furth et al. (1973) described them as chromophobic, secreting prolactin. Kovacs et al. (1977) in an ul trastructural investigation of adenomas of old female Long-Evans rats, found that they were composed of prolactin cells. Berkvens et al. (1980) using immunocytochemistry at the light microscopic level, demonstrated that some spontaneous tumors of old Wistar rats could contain GH, TSH or ACTH as well as PRL.

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