scholarly journals Microbiological Properties of Beejamrit, an Ancient Indian Traditional Knowledge, Uncover a Dynamic Plant-Beneficial Microbial Network

2021 ◽  
Author(s):  
Shibasis Mukherjee ◽  
Suchana Sain ◽  
Md Nasim Ali ◽  
Rupak Goswami ◽  
N Ravisankar ◽  
...  
Keyword(s):  
Plant Growth ◽  
Seed Treatment ◽  
Low Cost ◽  
Scientific Evidence ◽  
Foliar Spray ◽  
Plant Growth Promoting Rhizobacteria ◽  
Cow Dung ◽  
Beneficial Bacteria ◽  
Traditional Practice ◽  
Microbiological Properties

Abstract Beejamrit is an ancient organic formulation commonly used as a seed treatment in organic and natural farming in India. This low-cost input is primarily composed of cow dung, cow urine, and forest soil, which is often supplemented with limestone. In organic agriculture, it is a traditional practice among the farming community to incubate the seeds in Beejamrit prepared overnight before sowing in the field. However, a lack of substantial scientific evidence has been a major limitation to promote this farmers' innovative practice. On the other hand, growing data suggests that cow-based formulations are mostly enriched with microbial sources, including several plant growth-promoting rhizobacteria that are capable of synthesizing plant growth regulators. However, the microbiological properties of Beejamrit and their temporal changes over different periods of decomposition are largely unexplored. In this study, we aim to analyze the decomposition rate of Beejamrit over 7-consecutive days of incubation. This study further elucidates the microbial niche and their dynamics in Beejamrit, including the plant-beneficial bacteria. It was observed that the population of plant-beneficial bacteria such as the free-living nitrogen fixers and the phosphate solubilizers proliferates progressively up to 4-days of incubation. Similarly, the indolic compounds, including indole acetic acid, were enriched to the highest concentration in Beejamrit after 4-days of decomposition. Together, our analyses establish that Beejamrit provides a dynamic, microbe-based metabolic network and acts as a plant growth regulator. In conclusion, this study recommends the Beejamrit preparation after 4-days of incubation in the seed treatment and foliar spray to receive its optimum benefits.

scholarly journals Perlakuan Rizobakteri Pemacu Pertumbuhan Tanaman (RPPT) dengan Beberapa Tingkat Kerapatan Inokulum Rizobakteri Terhadap Viabilitas dan Vigor Benih Cabai Merah Kadaluarsa (Capsicum annuum L.)

2020 ◽  
Vol 4 (1) ◽  
pp. 229-238
Author(s):  
Dayang Rahmanita Simanjuntak ◽  
Halimursyadah Halimursyadah ◽  
Syamsuddin Syamsuddin
Keyword(s):  
Plant Growth ◽  
Capsicum Annuum ◽  
Seed Treatment ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Seed Vigor ◽  
Inoculum Density ◽  
Plant Growth Promoting ◽  
Actinobacillus Suis ◽  
Growth Promoting

Abstrak. Biological seed treatment merupakan salah satu perlakuan benih menggunakan mikroorganisme seperti rizobakteri pemacu pertumbuhan tanaman (RPPT). Penelitian ini bertujuan untuk mengetahui jenis rizobakteri dan kerapatan inokulum yang dapat meningkatkan viabilitas dan vigor benih cabai kadaluarsa. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan 2 faktor dan 3 ulangan. Faktor pertama adalah jenis rizobakteri (R) terdiri atas lima taraf yaitu R1: Necercia sp; R2:Bacillus polymixa; R3: Actinobacillus suis; R4: Azotobacter sp; R5: Pseudomonas capacia. Faktor kedua adalah kerapatan inokulum rizobakteri terdiri dari tiga taraf yaitu K1: 107 cfu/ml; K2: 108 cfu/ml; K3: 109 cfu/ml. Hasil penelitian ini menunjukkan bahwa perlakuan benih menggunakan rizobakteri jenis Necercia sp dengan kerapatan inokulum 108 cfu/ml nyata meningkatkan vigor benih pada tolok ukur  indeks vigor yaitu 40% dan Pseudomonas capacia dengan kerapatan inokulum 109 cfu/ml juga merupakan kombinasi perlakuan terbaik dalam meningkatkan berat kering kecambah normal yaitu 69,33 mg.Treatment Of Plant Growth Promoting Rhizobacteria (PGPR)With Multiple Levels of Rhizobacteria Inoculum Density On Viability and Vigor Of Expired Red Chilli Seeds (Capsicum annuum L.Abstract. Biological seed treatment is one of the seed treatment using microorganisms such as plant growth-promoting rhizobacteria (PGPR). This study aims to determine the type of rhizobacteria and inoculum density that can increase the viability and vigor of expired chili seeds. This research uses Completely Randomized Design (CRD) factorial pattern with 2 factors and 3 replications. The first factor is the type of rhizobacteria (R) consists of five levels, namely R1: Necercia sp; R2: Bacillus polymixa; R3: Actinobacillus suis; R4: Azotobacter sp; R5: Pseudomonas capacia. The second factor is the density of rhizobacteria inoculum consisting of three levels namely K1: 107 cfu/ml; K2: 108 cfu/ml; K3: 109 cfu/ml. The results of this study showed that the seed treatment using the Necercia sp-type rizobacteria with 108 cfu/ml inoculum density significantly increased the seed vigor on the vigor index benchmark of 40% and Pseudomonas capacia with 109cfu/ml inoculum density was also the best treatment combination in increasing dry weight normal sprout is 69,33 mg. 

Survival and colonization of rhizobacteria in a tomato transplant system

2003 ◽  
Vol 49 (6) ◽  
pp. 383-389 ◽  
Author(s):  
Zhinong Yan ◽  
M S Reddy ◽  
Joseph W Kloepper
Keyword(s):  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Seed Treatment ◽  
Bacillus Pumilus ◽  
Growth Promotion ◽  
Lateral Roots ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Colonization ◽  
Initial Inoculum ◽  
Application Method

Plant-growth-promoting rhizobacteria (PGPR) are used on crops most often as seed treatments; however, an alternative application method for transplanted vegetables is mixing PGPR into the soilless medium in which the transplants are grown. Studies were undertaken to compare root colonization and persistence of rifampicin-resistant mutants of PGPR strains Bacillus pumilus SE34 and Pseudomonas fluorescens 89B61, SE34r and 89B61r, on tomato as a function of application method. When the bacteria were incorporated into Promix(tm) soilless medium at log 6, 7, and 8 colony- forming units/g, populations of strain SE34r per gram of medium maintained the initial inoculum densities, while populations of 89B61r decreased approximately one to two orders of magnitude by 4 weeks after planting. The populations of each PGPR strain colonizing roots after application into the soilless medium showed a similar pattern at 6 weeks as that at 4 weeks after planting, with higher populations on the whole roots and lateral roots than on the taproots. Strain SE34r but not 89B61r moved upwards and colonized the phyllosphere when incorporated into the soilless medium. Following application as seed treatment, populations of SE34r were significantly higher on upper roots and on the taproot than were populations following application through the soilless medium. Conversely, populations were higher on lower roots and lateral roots following application through the soilless medium than were populations following application as seed treatment. While strain SE34 enhanced plant growth with application both to the medium and as seed treatment, the level of growth promotion was significantly greater with application in the soilless medium. The results indicate that PGPR can be successfully incorporated into soilless media in vegetable transplant production systems.Key words: rhizobacteria, plant colonization, Bacillus pumilus, Pseudomonas fluorescens.

scholarly journals Gibberellin and cytokinin effects on soybean growth

2003 ◽  
Vol 60 (3) ◽  
pp. 537-541 ◽  
Author(s):  
Vagner Maximino Leite ◽  
Ciro Antonio Rosolem ◽  
João Domingos Rodrigues
Keyword(s):  
Plant Growth ◽  
Root Growth ◽  
Leaf Area ◽  
Vegetative Growth ◽  
Seed Treatment ◽  
Dry Matter ◽  
Foliar Application ◽  
Foliar Spray ◽  
Stem Diameter ◽  
Joint Application

Soybean is an important crop in Brazil. Nonetheless, there are no reports on the use of plant growth regulator potential in relation to this crop in the national literature. To better understand the role of these compounds, a pot experiment was carried out to study effects of GA3 and cytokinin on the vegetative growth of the soybean. GA3 (50 mg L-1) was applied as seed treatment, leaving plants with water application as control. GA3 (100 mg L-1) and cytokinin (30 mg L-1) were sprayed on leaves at the physiological stage V3/V4, and 15 days after, cytokinin (30 mg L-1), also as foliar spray. Seed treatment decreased plant emergence and initial soybean root growth, but as the season progressed, differences in root growth disappeared; plants were shorter, and presented a decrease in the number of nodes, in stem diameter, in leaf area and in dry matter yield. Conversely, foliar application of GA3 led to an increase in plant height, first node height and stem diameter. Leaf area and dry matter production also increased as a result of GA3 foliar application. There was no effect of exogenous gibberellin and cytokinin on the number of soybean leaves, number of stem branches and root dry matter. Joint application of gibberellin and cytokinin tended to inhibit gibberellin effects. Cytokinin applied to leaves during soybean vegetative growth was not effective in modifying any of the evaluated plant growth variables.

scholarly journals Role of Plant Growth-Promoting Rhizobacteria (PGPR) and Bio-Control Agents (BCAs) in Crop Production

2020 ◽  
Vol 7 (3) ◽  
pp. 144-150
Author(s):  
Sujata Kumari ◽  
◽  
Narender K. Bharat ◽  
Ashok K. Thakur ◽  
◽  
...  
Keyword(s):  
Plant Growth ◽  
Seed Treatment ◽  
Crop Production ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Diseases ◽  
Environment Friendly ◽  
Plant Growth Promoting ◽  
Growth Promoting

The bioagents like Plant Growth Promoting Rhizobacteria (PGPR) and Biocontrol Agents (BCAs) play a crucial role in plant growth promotion, nutrient uptake and suppression of biotic and abiotic stresses. Different researchers have applied these bioagents by various means either through seed treatment or through soil application to prevent various plant diseases. Thus, these non-chemical environment friendly tools can be exploited to enhance crop production.

scholarly journals Influence of Seed Hardening and Foliar Spraying of PGRs on Morpho-physiological Parameters in Green Gram (Vigna radiata L.)

2021 ◽  
Author(s):  
R.S. Bhadane ◽  
K.R. Prajapati ◽  
S.D. Rajput ◽  
Kalyanrao Patil
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Growth Parameters ◽  
Low Cost ◽  
Foliar Spray ◽  
Physiological Parameters ◽  
Green Gram ◽  
Yield Potential ◽  
Sowing Seed

Background: Pre-sowing seed hardening and foliar spray with plant growth regulators is an easy, low cost and low risk technique and also an alternative approach recently used to mitigate the effect of abiotic stresses in agricultural production. Methods: The mung bean var. GAM-5 was treated with seed hardening treatments and also for foliar spray using CaCl2 2% and 1%, CCC 500 mg/L, CCC 1000 mg/L, NAA 25 mg/L, NAA 50 mg/L. during summer season of 2015-16 and 2016-17. The trial was laid out in RBD with three replications and sixteen treatment combinations. Result: The results indicated a significant improvement in morpho-physiological growth parameters, dry matter production and thereby yield potential increased due to the application of plant growth regulators and agrochemical under field conditions. Among the different treatments, seed hardening with 2% CaCl2 + 1% foliar spraying at 30 DAS (T11) treatment significantly improved most of morpho-physiological parameters and thereby yield in green gram followed by the seed hardening treatments of Cycocel 1000 mg/L + foliar spraying at 30 DAS (T13) and seed hardening with NAA 50 mg/L + foliar spraying at 30 DAS (T15).

scholarly journals IMPROVING THE QUALITY OF ORNAMENTAL BULBOUS WITH PLANT GROWTH-PROMOTING RHIZOBACTERIA (PGPR)

2021 ◽  
pp. 225-263
Author(s):  
Domenico Prisa ◽  
Alessandra Benati
Keyword(s):  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Root Weight ◽  
Beneficial Bacteria ◽  
Effective Microorganisms ◽  
Soil Microbial ◽  
Potted Plants ◽  
Growing Medium ◽  
Plant Growth Promoting ◽  
Growth Promoting

The aim of this work was to use Plant growth promoting rhizobacteria (PGPR) for the improvement of cultivation and agronomic and pathogen protection characteristics of ornamental bulbous plants such as Tulip (fam. Liliacee), Iris (fam. Iridacee), Freesia (fam. Iridacee) and Narcissus (fam. Amarillidacee). The experiments, started in November 2020, were conducted in the greenhouses of CREA-OF in Pescia (Pt), Tuscany, Italy. The experimental groups were: i) group control irrigated with water and substrate previously fertilized; ii) group with Effective microorganisms irrigated with water and substrate previously fertilized; iii) group with beneficial bacteria (TNC Bactorrs13) irrigated with water and substrate previously fertilized; iv) group with beneficial bacteria (Tarantula powder Advanced nutrients ) irrigated with water and substrate previously fertilized. The trial showed a significant improvement in the agronomic parameters analysed on plants obtained from Narcissus, Iris, Tulip and Freesia bulbs treated with microorganisms. In particular, there was an increase in plant height, vegetative and root weight, bulb weight and diameter, and flower duration. In addition, the use of microorganisms in the growing medium showed a significant increase in the biocontrol of Botrytis cinerea. The treatment with Effective microorganisms (EM) showed the most significant results for all agronomic parameters analysed. The improvement in plant growth brought about by the activity of bacteria is a key aspect of achieving sustainable agricultural goals in the future. More research is needed in this field to identify new soil microbial strains that can be used for the formulation of new products applicable for improving the quality and resistance to biotic and abiotic stresses of potted plants. KEY-WORDS: Ornamental bulbs; Microorganisms; Sustainable agriculture; Flowers; Pot plants

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