Calcium and phosphorus co-doped carbon dots enhance osteogenic differentiation for calvarial defect repair in situ

Abstract Calvarial bone defect remains a clinical challenge due to the lack of efficient osteo-inductive agent. Herein, a novel calcium and phosphorus codoped carbon dot (Ca/P-CD) for bone regeneration was synthesized using phosphoethanolamine and calcium gluconate as precursors. The resultant Ca/P-CDs exhibited ultra-small size, stable excitation dependent emission spectra and favorable dispersibility in water. Moreover, Ca/P-CDs with good biocompatibility rapidly entered the cytoplasm through endocytosis and increased the expression of bone differentiation genes. After mixing with temperature-sensitive hydrogel, Ca/P-CDs were injected in situ into calvarial defect and promoted the repair of bone injury. These Ca/P-CDs provide a new treatment method for the bone repair and expend the application in the biomedical fields.

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Regeneration of Bone Defects Using Bioactive Glass Combined with Adipose-derived Mesenchymal Stem Cells. An experimental in vivo study

Revista de Chimie ◽

10.37358/rc.19.6.7259 ◽

2019 ◽

Vol 70 (6) ◽

pp. 1983-1987

Author(s):

Cristian Trambitas ◽

Anca Maria Pop ◽

Alina Dia Trambitas Miron ◽

Dorin Constantin Dorobantu ◽

Flaviu Tabaran ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bioactive Glass ◽

Bone Repair ◽

Bone Defects ◽

Calvarial Bone ◽

Specific Protocol ◽

Repair Mechanisms ◽

Male Wistar Rats

Large bone defects are a medical concern as these are often unable to heal spontaneously, based on the host bone repair mechanisms. In their treatment, bone tissue engineering techniques represent a promising approach by providing a guide for osseous regeneration. As bioactive glasses proved to have osteoconductive and osteoinductive properties, the aim of our study was to evaluate by histologic examination, the differences in the healing of critical-sized calvarial bone defects filled with bioactive glass combined with adipose-derived mesenchymal stem cells, compared to negative controls. We used 16 male Wistar rats subjected to a specific protocol based on which 2 calvarial bone defects were created in each animal, one was filled with Bon Alive S53P4 bioactive glass and adipose-derived stem cells and the other one was considered control. At intervals of one week during the following month, the animals were euthanized and the specimens from bone defects were histologically examined and compared. The results showed that this biomaterial was biocompatible and the first signs of osseous healing appeared in the third week. Bone Alive S53P4 bioactive glass could be an excellent bone substitute, reducing the need of bone grafts.

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The Selection of Treatment Modality for Breast Ductal Carcinoma In Situ: Experience From a Single Institution

Cancer Control ◽

10.1177/1073274821997426 ◽

2021 ◽

Vol 28 ◽

pp. 107327482199742

Author(s):

Kai-yun You ◽

Zhuo-fei Bi ◽

Yu-jia Ma ◽

Yong-lin Mao ◽

Wei-liang Zou ◽

...

Keyword(s):

Local Recurrence ◽

Ductal Carcinoma In Situ ◽

Adjuvant Radiotherapy ◽

Carcinoma In Situ ◽

Ductal Carcinoma ◽

Multivariable Analysis ◽

Tumor Grade ◽

Treatment Method ◽

Free Survival

Purpose: Although breast conservation surgery(BCS) followed by adjuvant radiotherapy is now the mainstream treatment method for breast ductal carcinoma in situ(DCIS), mastectomy is still performed in some patients who refuse to undergo radiation. However, the most effective treatment method for these patients is still unknown. In the current study, we aimed to compare the survival rates between mastectomy and BCS plus adjuvant radiotherapy in patients with DCIS. Materials and Methods: We performed a retrospective study of 333 patients with DCIS from May 2004 to December 2016. There were 209 patents who were treated with BCS and adjuvant radiotherapy, while the remaining of 124 patients underwent mastectomy. The disease-free survival (DFS) and local recurrence-free survival(LRFS) rates were compared between the 2 treatment groups. Cox proportional hazards regression was performed to explore factors associated with DFS and LRFS. Results: The 10-year local recurrence(LR) rates in the mastectomy and BCS plus adjuvant radiotherapy groups were 2.6% and 7.5%, respectively. There was no difference in the LR rate between the 2 groups. Furthermore the DFS rate was also similar between the mastectomy and BCS plus adjuvant radiotherapy groups. Based on the multivariable analysis, age and tumor grade were significantly correlated with the LRFS and DFS rates. In the subgroup analysis based on the factors of age and tumor grade, patients with a tumor grade of III who underwent mastectomy had better LRFS and DFS rates compared to those who received BCS plus radiotherapy. Conclusion: In patients with DCIS, the long-term efficacy was similar between mastectomy and BCS followed by adjuvant radiotherapy. However, in the subgroup of patients with grade III tumors, mastectomy seems to offer a better LRFS and DFS than BCS plus radiotherapy.

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Influence of Calcination Temperature on Crystal Growth and Optical Characteristics of Eu3+ Doped ZnO/Zn2SiO4 Composites Fabricated via Simple Thermal Treatment Method

Crystals ◽

10.3390/cryst11020115 ◽

2021 ◽

Vol 11 (2) ◽

pp. 115

Author(s):

Suhail Huzaifa Jaafar ◽

Mohd Hafiz Mohd Zaid ◽

Khamirul Amin Matori ◽

Sidek Hj. Ab Aziz ◽

Halimah Mohamed Kamari ◽

...

Keyword(s):

Thermal Treatment ◽

Band Gap ◽

Calcination Temperature ◽

Emission Intensity ◽

Emission Spectra ◽

Treatment Method ◽

Absorption Bands ◽

Vis Spectroscopy ◽

Doped Zno ◽

Zno Crystal

This research paper proposes the usage of a simple thermal treatment method to synthesis the pure and Eu3+ doped ZnO/Zn2SiO4 based composites which undergo calcination process at different temperatures. The effect of calcination temperatures on the structural, morphological, and optical properties of ZnO/Zn2SiO4 based composites have been studied. The XRD analysis shows the existence of two major phases which are ZnO and Zn2SiO4 crystals and supported by the finding in the FT-IR. The FESEM micrograph further confirms the existence of both ZnO and Zn2SiO4 crystal phases, with progress in the calcination temperature around 700–800 °C which affects the existence of the necking-like shape particle. Absorption humps discovered through UV-Vis spectroscopy revealed that at the higher calcination temperature effects for higher absorption intensity while absorption bands can be seen at below 400 nm with dropping of absorption bands at 370–375 nm. Two types of band gap can be seen from the energy band gap analysis which occurs from ZnO crystal and Zn2SiO4 crystal progress. It is also discovered that for Eu3+ doped ZnO/Zn2SiO4 composites, the Zn2SiO4 crystal (5.11–4.71 eV) has a higher band gap compared to the ZnO crystal (3.271–4.07 eV). While, for the photoluminescence study, excited at 400 nm, the emission spectra of Eu3+ doped ZnO/Zn2SiO4 revealed higher emission intensity compared to pure ZnO/Zn2SiO4 with higher calcination temperature exhibit higher emission intensity at 615 nm with 700 °C being the optimum temperature. The emission spectra also show that the calcination temperature contributed to enhancing the emission intensity.

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The Vyredox and Nitredox Methods of in situ Treatment of Groundwater

Water Science & Technology ◽

10.2166/wst.1988.0093 ◽

1988 ◽

Vol 20 (3) ◽

pp. 149-163 ◽

Cited By ~ 30

Author(s):

Carol Braester ◽

Rudolf Martinell

Keyword(s):

Water Treatment ◽

Surface Water ◽

Traditional Method ◽

Treatment Method ◽

Nitrate Treatment ◽

The Past ◽

The World ◽

Nitrate And Nitrite ◽

Iron And Manganese

Nearly one fifth of all water used in the world is obtained from groundwater. The protection of water has become a high priority goal. During the last decades pollution of water has become more and more severe. Today groundwater is more and more used in comparison with surface water. Recently we have seen accidents, which can pollute nearly all surface water very quickly. Generally the groundwater is easier to protect, as well as cheaper to purify, and above all it is of better quality than the surface water. During the past two decades, alternatives to the traditional method of treating the water in filters have been developed, that is in situ water treatment i.e. the VYREDOX and NITREDOX methods. The most common problem regarding groundwater is too high content of iron and manganese, which can be reduced with the VYREDOX method. In some areas today there are severe problems with pollution by hydrocarbons and nitrate as well, and with modification of the VYREDOX treatment method it is used for hydrocarbon and nitrate treatment as well. The method to reduce the nitrate and nitrite is known as the NITREDOX method.

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Direct contribution of skeletal muscle mesenchymal progenitors to bone repair

Nature Communications ◽

10.1038/s41467-021-22842-5 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Anais Julien ◽

Anuya Kanagalingam ◽

Ester Martínez-Sarrà ◽

Jérome Megret ◽

Marine Luka ◽

...

Keyword(s):

Skeletal Muscle ◽

Bone Healing ◽

Bone Repair ◽

Fibrotic Response ◽

Mesenchymal Progenitors ◽

Fibrotic Tissue ◽

Bone Injury ◽

Single Cell Rna Sequencing ◽

Musculoskeletal Trauma

AbstractBone regenerates by activation of tissue resident stem/progenitor cells, formation of a fibrous callus followed by deposition of cartilage and bone matrices. Here, we show that mesenchymal progenitors residing in skeletal muscle adjacent to bone mediate the initial fibrotic response to bone injury and also participate in cartilage and bone formation. Combined lineage and single-cell RNA sequencing analyses reveal that skeletal muscle mesenchymal progenitors adopt a fibrogenic fate before they engage in chondrogenesis after fracture. In polytrauma, where bone and skeletal muscle are injured, skeletal muscle mesenchymal progenitors exhibit altered fibrogenesis and chondrogenesis. This leads to impaired bone healing, which is due to accumulation of fibrotic tissue originating from skeletal muscle and can be corrected by the anti-fibrotic agent Imatinib. These results elucidate the central role of skeletal muscle in bone regeneration and provide evidence that skeletal muscle can be targeted to prevent persistent callus fibrosis and improve bone healing after musculoskeletal trauma.

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In situ strategy for bone repair by facilitated endogenous tissue engineering

Colloids and Surfaces B Biointerfaces ◽

10.1016/j.colsurfb.2015.08.019 ◽

2015 ◽

Vol 135 ◽

pp. 581-587 ◽

Cited By ~ 9

Author(s):

Jingdi Chen ◽

Yujue Zhang ◽

Panpan Pan ◽

Tiantang Fan ◽

Mingmao Chen ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Repair

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NIR-II Ratiometric Lanthanide-Dye Hybrid Nanoprobes Doped Bioscaffolds for In Situ Bone Repair Monitoring

Nano Letters ◽

10.1021/acs.nanolett.1c04356 ◽

2022 ◽

Author(s):

Peng Pei ◽

Hongxing Hu ◽

Ying Chen ◽

Shangfeng Wang ◽

Jing Chen ◽

...

Keyword(s):

Bone Repair

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The Formation of an Epitaxial-Graphene Cap Layer for Post-Implantation High Temperature Annealing of SiC and its In Situ Removal by Si-Vapor Etching

Materials Science Forum ◽

10.4028/www.scientific.net/msf.679-680.777 ◽

2011 ◽

Vol 679-680 ◽

pp. 777-780 ◽

Cited By ~ 4

Author(s):

Shoji Ushio ◽

Ayumu Adachi ◽

Kazuhiro Matsuda ◽

Noboru Ohtani ◽

Tadaaki Kaneko

Keyword(s):

High Temperature ◽

Graphene Layer ◽

Treatment Method ◽

Etch Depth ◽

High Temperature Annealing ◽

Large Area ◽

Vapor Flux ◽

Precise Control ◽

Post Implantation

As a new graphene functionality applicable to post-implantation high temperature annealing of SiC, a method of in situ formation and removal of large area epitaxial few-layer graphene on 4H-SiC(0001) Si-face is proposed. It is demonstrated that the homogeneous graphene layer formed by Si sublimation can be preserved without the decomposition of the underlying SiC substrate even in the excess of 2000 oC in ultrahigh vacuum. It is due to the existence of the stable (6√3×6√3) buffer layer at the interface. To ensure this cap function, the homogeneity of the interface must be guaranteed. In order to do that, precise control of the initial SiC surface flatness is required. Si-vapor etching is a simple and versatile SiC surface pre/post- treatment method, where thermally decomposed SiC surface is compensated by a Si-vapor flux from Si solid source in the same semi-closed TaC container. While this Si-vapor etching allows precise control of SiC etch depth and surface step-terrace structures, it also provides a “decap” function to remove of the graphene layer. The surface properties after the each process were characterized by AFM and Raman spectroscopy.

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