scholarly journals Mucosal Immune Responses and Total Carotenoid Content of Juvenile Male Guppy Fish (Poecilia Reticulata) Fed With Different Herbal Supplements ​

Author(s):  
Seyed Nesa Mousavi ◽  
Hamid Mohammadiazarm ◽  
Fatemeh Azadbakht

Abstract In this experiment, the influences of dietary garlic, onion, and sweet red pepper meals as herbal supplements were studied on some immunological parameters, total carotenoid content, and growth performance of male guppy fish (Poecilia reticulata) in the juvenile stage. The experimental diets were fed to fish as a control (0), and three other diets contained 20 g kg− 1 garlic, or 20 g kg− 1 red onion, and or 20 g kg− 1 sweet red pepper powders. The male guppies with the early weight of 0.642g ± 0.07 were stocked in twelve circular tanks (40 L capacity) at density of 30 fish per tank. Fish were fed to satiation the diets thrice daily. The results showed that dietary herbal additives induced a significant increase (P < 0.05) in some mucosal immunological parameters contained alternative complement (ACH50), total immunoglobulin (Ig), lysozyme, and total protein compared to control, which were the highest in the group with 20 g kg− 1 garlic powder. Also, various dietary herbal additives resulted in higher (P < 0.05) total carotenoids levels of lateral skin body and caudal fin of fish than control, which were the highest in the group with 20 g kg− 1 sweet red pepper. The growth performance and feeding indices enhanced significantly (P < 0.05) in experimental groups compared to the control. As a result, used herbal supplements had beneficial effects on immunological parameters and total carotenoid content of fish. So, the highest immunity and coloration was obtained separately at 20 g kg− 1 respectively with garlic and sweet red pepper meals.

2012 ◽  
Vol 24 (1) ◽  
pp. 33-39
Author(s):  
Wanda Wadas ◽  
Halina Mioduszewska ◽  
Krzysztof Kalinowski

Effects of selected agronomical factors on the content of health-promoting compounds in spaghetti squash (Cucurbita pepoL. subsp.pepo)The study examined the effects of the sowing date (5, 15 and 25 May) and plant spacing (1 × 0.6 m, 1 × 0.8 m and 1 × 1 m) on the content of carotenoids and L-ascorbic acid in the fruits of two cultivars of spaghetti squash (‘Makaronowa Warszawska' and ‘Pyza’). The field experiment was carried out in central-eastern Poland. Fully mature spaghetti squash fruits were harvested from the beginning of September to the beginning of October. The content of total carotenoids and β-carotene was the greatest in the fruits of plants sown on 15 May. The sowing date did not affect the content of L-ascorbic acid. Increasing the plant spacing from 1 × 0.6 m to 1 × 1 m resulted in an increase of total carotenoid content, particularly for the spaghetti squash sown on 25 May. Plant spacing did not have a significant effect on the content of β-carotene and L-ascorbic acid. Fruits of the ‘Makaronowa Warszawska’ with light orange flesh contained more total carotenoids and β-carotene than fruits of the ‘Pyza’ with cream-coloured flesh. The content of L-ascorbic acid in the fruits of both cultivars did not differ significantly.


Author(s):  
Alena Andrejiová ◽  
Alžbeta Hegedűsová ◽  
Miroslav Šlosár ◽  
Silvia Barátová

The winter squash is an important source of antioxidants, especially carotenoids. The aim of submitted research work was to determine the effect of genotype, storage and different methods of technological processing (baking, boiling and sterilization) on the content of ascorbic acid and total carotenoids in fruits of winter squash (Cucurbita moschata Duch. ex Poir.). The small-plot field experiment was established at Slovak University of Agriculture in Nitra in 2013. Five cultivars of winter squash (‘Liscia’, ‘Orange’, ‘Hannah’, ‘UG 205 F1’ and ‘Waltham’) were examined in experiment. The total carotenoids content in the pulp of fresh fruits was ranged from 9.33 to 15.10 mg.100 g−1. Its highest value was determined in case of ‘Orange’ variety. The storage and the thermal treatment of fruit pulp in case of baking had positive impact from the total carotenoid content point of view. The baking resulted in the increase of its value in winter squash. On the contrary, sterilization tended to the decrease of total carotenoid content in edible part of squash. The total carotenoids content in the baking pulp was ranged from 14.27 to 31.87 mg.100 g−1. The vitamin C content before storage and technological processing ranged in interval from 13.88 to 18.69 mg.100 g−1. Particular thermal methods of processing and storage resulted in decrease of vitamin C content in the pulp of all winter squash varieties.


2015 ◽  
Vol 43 (3) ◽  
pp. 255-260
Author(s):  
Gyanendra Kumar Rai ◽  
Rajesh Kumar ◽  
Ranjeet Ranjan Kumar ◽  
Sheetal Dogra

Significant differences (p ? 0.05) were observed among the cherry tomato lines for the principal antioxidants, viz. total carotenoids, lycopene and vitamin-C. Vitamin-C content ranged from 17.62 - 46.16 mg/100 g, the total carotenoid content ranged from 3.86 - 6.66 mg/100 g and lycopene content ranged from 2.83 - 5.26 mg/100 g on fresh weight basis. The total phenolics, another important class of antioxidants, also differed significantly amongst the cherry tomato line (12.41-31.17 mg gallic acid equivalent/100 g). Significant variation (p ? 0.05) was also observed for pH and titrable acidity. The pH varied from 4.15 - 4.52 and anhydrous citric acid ranged from 0.050 up to 0.323%. The total soluble solids were 3.41 - 5.16%. The maximum vitamin-C content was recorded in VRCT-6 (46.16 mg/100 g) closely followed by VRCT-7 (45.51 mg/100 g) and VRCT-15 (44.71 mg/100 g), whereas maximum total carotenoid content were recorded in VRCT-16 (6.66 mg/100 g) followed by VRCT-7 (6.48 mg/100 g) and VRCT-1(6.36 mg/100 g), respectively. On the other hand, maximum lycopene content was estimated in VRCT-16 (5.26 mg/100 g) followed by VRCT-3 (5.23 mg/100 g) and VRCT-14 (4.73 mg/100 g). Maximum acidity and total water soluble solids were recorded in VRCT-9 (0.323%) and VRCT-7 (5.16%), respectively. DOI: http://dx.doi.org/10.3329/bjb.v43i3.21591 Bangladesh J. Bot. 43(3): 255-260, 2014 (December)


Beverages ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 43
Author(s):  
Stavros Lalas ◽  
Aggeliki Alibade ◽  
Eleni Bozinou ◽  
Dimitris P. Makris

In this study, peach pomace (PP) moisture reduction using conventional oven-drying was investigated by implementing the Box-Behnken experimental design, considering two major process variables, time—t and temperature—T. The purpose was the optimisation of the process to obtain PP extracts as rich as possible in total carotenoids (TCn). It was shown that effective moisture removal up to a final level of approximately 24%, could be achieved after 8.27 h (496 min) at 70 °C. Under these optimised drying conditions, the maximum carotenoid yield was 84.57 ± 8.56 μg CtE g−1 dm. This yield was by almost 63% lower than that achieved using fresh (non-dried) samples. Temperatures higher than 70 °C were demonstrated to be even more detrimental in this regard, yet from the model built, it was made clear that prolonged drying time may bring about a more pronounced negative effect on the total carotenoid yield. The drop in total carotenoid content of PP as a result of drying was accompanied by a significant decline in the antiradical activity of PP extracts.


Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 196
Author(s):  
Ancuta Nartea ◽  
Benedetta Fanesi ◽  
Pasquale Massimiliano Falcone ◽  
Deborah Pacetti ◽  
Natale Giuseppe Frega ◽  
...  

The effect of steam and sous-vide oven procedures on liposoluble antioxidants of colored cauliflower (orange and purple) was assessed for the first time and compared with domestic practice (boiling). In raw samples, the total carotenoid content was 10-fold higher in Cheddar than in Depurple (20.9 ± 2.1 vs. 2.3 ± 0.5 mg/kg dry weight), whereas the level of tocopherols was similar (28.5 ± 4.4 vs. 33 ± 5.2 mg/kg dry weight). The Cheddar liposoluble antioxidant matter contained violaxanthin, neoxanthin, α-carotene and δ-tocopherol, not detected in Depurple. All tests increased the bioactive compounds extractability with steam oven and sous-vide displaying similar effects, lower than boiling. In boiled Cheddar cauliflower, the total carotenoids and tocopherols contents increased with cooking time until they were 13-fold and 6-fold more than in raw cauliflower, respectively. Conversely, in the Depurple variety, contents increased by half with respect to the orange variety. However, from a nutritional point of view, no differences were revealed among the three different cooking treatments in terms of vitamin A and E levels expressed in μg/100 g of fresh vegetable because of the higher water content of boiled samples that must be considered when evaluating the effect of thermal treatment on cauliflower nutritional traits.


2011 ◽  
Vol 32 (3) ◽  
pp. 279-287 ◽  
Author(s):  
Sanghdeep Gautam ◽  
Jaswant Singh ◽  
Aditya Pant

Effect of UV-B radiations on the pigments of two Antarctic lichens of Schirmacher Oasis, East Antarctica Antarctic plants experience UV-B stress and for their survival they have been showing various adaptive strategies. The first line of defence is to screen UV-B radiation before it reaches the cell, then to minimize damage within the cells through other protective strategies, and finally to repair damage once it has occurred. A fifteen days experiment was designed to study lichen: Dermatocarpon sp. and Acarospora gwynnii under natural UV and below UV filter frames in the Indian Antarctic Station Maitri region of Schirmacher Oasis, East Antarctica. Changes in UV absorbing compounds, total phenolics, total carotenoids and chlorophyll content were studied. The change in total phenolics and total carotenoid content was significant in both Dermatocarpon sp. and A. gwynnii indicating that the increase in UV absorbing compounds, total phenolics and total carotenoid content act as a protective mechanism against the deleterious effect of UV-B radiations, whereas the change in chlorophyll content was not significant in both lichen species.


HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 398C-398
Author(s):  
Marisa Wall ◽  
Cynthia Waddell ◽  
Paul Bosland ◽  
Stephanie Walker

The β-carotene and total carotenoid content of different Capsicum fruit types and species were analyzed using HPLC. This information is useful for breeding high carotenoid chiles (New Mexican type) for the food industry, and also provides nutritional data for the range of fruit types within the Capsicum genus. Fresh fruit from 25 accessions and dried fruit from 39 accessions were evaluated in 1996 and 1997. β-carotene levels varied from 0 to 16.6 mg/100 g fresh weight, and carotenoid levels were from 0.1 to 89.6 mg/100 g in red ripe fruit in 1996. The range of values for β-carotene was similar in 1997, but a wider range in total carotenoids (0.4 to 117.3 mg/100 g fresh weight) was observed. Fresh fruit (100 g) of the cultivars `Greenleaf Tabasco', `Pulla', `Guajillo', `NuMex Conquistador', `Ring-O-Fire', and `Thai Dragon' contained greater amounts of β-carotene than the RDA for vitamin A for the average adult. For dried Capsicum entries, New Mexican, aji, pasilla, ancho, and guajillo types had the highest levels of β-carotene. In 1996, β-carotene levels among the dried Capsicum germplasm ranged from 0 to 739.2 μg/g dry weight, and carotenoid levels were from 21.3 to 6,225.9 μg/g. Values were higher in 1997, and ranged from 23.7 to 1,198.1 μg/g dry weight for β-carotene and from 76.9 to 10,120.6 μg/g for total carotenoids. A pasilla type (C. annuum) had the highest total carotenoid content among the dried entries in both years.


2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Adriána Maťová ◽  
Alžbeta Hegedűsová ◽  
Alena Andrejiová ◽  
Ondrej Hegedűs ◽  
Marcel Golian ◽  
...  

Cucurbita moschata Duch. is a seasonal crop, appreciated for its nutrimental and bioactive components especially carotenoids. From the whole edible plant, fruit is most often consumed which can be stored and also processed. Six varieties—Liscia, Matilda, Orange, Serpentine, UG 205 F1, and Waltham—of Cucurbita moschata Duch. were investigated, in order to analyze and evaluate the changes in total carotenoids content in the pulp during storage and after the effect of heat treatments—freezing, baking, and cooking (water boiling). The average total carotenoids content ranged from 3.32 mg/100 g (Serpentine) to 9.35 mg/100 g fresh matter (FM) (Orange). After 60 days of storage, a slight increase in the total carotenoids content of all analyzed varieties was observed. The values of the monitored varieties ranged from 4.18 mg/100 g FM (Serpentine), which represents a 26% increase to 10.96 mg/100 g FM (Orange), where a 17% increase was observed. After 120 days of storage, the results were mixed. In some varieties (Liscia and Matilda), the total carotenoid content decreased, while in the varieties Serpentine, UG 205 F1, and Waltham, its content increased slightly (11%, 3%, and 11%), but the content of the Orange variety remained unchanged. The total carotenoids increment after 60 and 120 days of storage in the dry matter (DM) was statistically significant, as well as the effect of the variety. The total carotenoid content of cooked samples of all varieties increased, from 119.78 mg/100 g DM (Waltham) to 255.19 mg/100 g DM (Orange). After baking, an average of 12% increase in the total content of carotenoids was recorded, after freezing, a decrease in its level of 5% was indicated. The findings of this work show that Cucurbita moschata Duch. is a good source of carotenoids, even after several weeks of storage and after exposure to heat treatments.


2020 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Hanny Wijaya ◽  
Natania Natania ◽  
Mei Diana Sonatha ◽  
Qurrata A'yuni ◽  
Cindy Caroline

Red fruits’ oil contains 12,000 ppm carotenoid compounds and among them β-karoten was found as the major carotenoid compound. However, the application of red fruits’ oil as the food ingredient is rarely investigated due to its low solubility in aqua-phase, phenolic aroma and bitter aftertaste. Therefore , emulsification of red fruits’ oil in water (oil in water, o/w emulsion) using CMC (carboxymethyl cellulose) as the emulsifier and stabilizer agent considered as alternative to  overcome those limitations. In the present work, the optimum concentration of red fruits’ oil emulsion added into Java tea-based  functional drink increased the  total carotenoid content,  gave red color and might enhanced its antioxidant activity. The optimum formula was performed  by  Response Surface Methodology approach utilizing Design Expert 10.0.7® (DX10) Trial software whereas the emulsion concentration of red fruits’ oil as the variable factor. Red fruits’ oil emulsion which has been prepared  from 20% red fruits’ oil and 1.50% CMC as emulsifier and stabilizer was having total carotenoid content of 72.93±1.33 ppm. The optimum concentration of red fruits’ oil emulsion added to  Java-tea based functional drink  was 5.86%. The addition of red fruits’ oil emulsion increased the total carotenoid content from 0.37±0.13 ppm to5.28±0.05 ppm. The results showed that red fruits’ oil emulsion could be used as red colorant agent in the functional drink while increasing the total carotenoid content, however, reduced the flavor sensory acceptance in high amount


HortScience ◽  
2001 ◽  
Vol 36 (4) ◽  
pp. 746-749 ◽  
Author(s):  
Marisa M. Wall ◽  
Cynthia A. Waddell ◽  
Paul W. Bosland

The β-carotene and total carotenoid content of either fresh or dried tissue of fruits of a total of 57 cultivars of six Capsicum species were analyzed using high performance liquid chromatography (HPLC). β-Carotene levels in ripe fruit varied from 0 to 166 μg·g-1 fresh weight, and carotenoid levels were from 1 to 896 μg·g-1 in ripe fruit in 1996. The range of values for β-carotene was similar in 1997, but that for total carotenoids was wider (4 to 1173 μg·g-1 fresh weight). Fresh fruit of the cultivars Greenleaf Tabasco, Pulla, Guajillo, NuMex Conquistador, Ring-O-Fire, and Thai Dragon contained greater amounts of β-carotene per 100 g fresh weight than the recommended dietary allowance (RDA) for vitamin A for the average adult. For dried Capsicum entries, New Mexican, aji, pasilla, and ancho types had the highest levels of β-carotene. In 1996, β-carotene levels among the dried Capsicum germplasm ranged from 2 to 739 μg·g-1 dry weight, and carotenoid levels from 111 to 6226 μg·g-1. Values were higher in 1997, ranging from 24 to 1198 μg·g-1 dry weight for β-carotene and from 187 to 10,121 μg·g-1 for total carotenoids. A pasilla type (C. annuum L.) had the highest total carotenoid content among the dried entries in both years.


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