scholarly journals Bioinformatics-Aided Identification, Characterization of Fungal Linalool Synthases and Applications in Linalool biosynthesis

2020 ◽  
Author(s):  
Congqiang Zhang ◽  
Xixian Chen ◽  
Raphael Tze Chuen Lee ◽  
Rehka T ◽  
Sebastian Mauer-Stroh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Phylogenetic Analysis ◽  
Metabolic Engineering ◽  
Bioinformatics Analysis ◽  
Personal Care Products ◽  
Divergent Evolution ◽  
Personal Care ◽  
Structural Comparison ◽  
Key Residues

Abstract Enzymes empower chemical industries and are the keystone for metabolic engineering. For example, linalool synthases (LSs) are indispensable for the biosynthesis of linalool, an important fragrance used in 60-80% cosmetic and personal care products. However, plant LSs have low activities while expressed in microbes. Aided by bioinformatics analysis, four linalool/nerolidol synthases (LNSs) from various Agaricomycetes were accurately predicted and validated experimentally. Furthermore, we discovered a novel LS with exceptionally high levels of selectivity and activity from Agrocybe pediades, ideal for linalool bioproduction. It effectively converted glucose into enantiopure (R)-linalool in Escherichia coli, 44-fold and 287-fold more efficient than bacterial and plant counterparts, respectively. Phylogenetic analysis indicated the divergent evolution paths for plant, bacterial and fungal LSs. More critically, structural comparison provided catalytic insights in Ap.LS superior specificity and activity, and mutational experiments validated the key residues responsible for the specificity.

scholarly journals Bioinformatics-aided identification, characterization and applications of mushroom linalool synthases

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Congqiang Zhang ◽  
Xixian Chen ◽  
Raphael Tze Chuen Lee ◽  
Rehka T ◽  
Sebastian Maurer-Stroh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Phylogenetic Analysis ◽  
Metabolic Engineering ◽  
Bioinformatics Analysis ◽  
Personal Care Products ◽  
Divergent Evolution ◽  
Personal Care ◽  
Structural Comparison ◽  
Key Residues ◽  
Linalool Synthase

AbstractEnzymes empower chemical industries and are the keystone for metabolic engineering. For example, linalool synthases are indispensable for the biosynthesis of linalool, an important fragrance used in 60–80% cosmetic and personal care products. However, plant linalool synthases have low activities while expressed in microbes. Aided by bioinformatics analysis, four linalool/nerolidol synthases (LNSs) from various Agaricomycetes were accurately predicted and validated experimentally. Furthermore, we discovered a linalool synthase (Ap.LS) with exceptionally high levels of selectivity and activity from Agrocybe pediades, ideal for linalool bioproduction. It effectively converted glucose into enantiopure (R)-linalool in Escherichia coli, 44-fold and 287-fold more efficient than its bacterial and plant counterparts, respectively. Phylogenetic analysis indicated the divergent evolution paths for plant, bacterial and fungal linalool synthases. More critically, structural comparison provided catalytic insights into Ap.LS superior specificity and activity, and mutational experiments validated the key residues responsible for the specificity.

Synthesis and Characterization of Active Biocarbon Material for Use in Cosmetics and Personal Care Products

2020 ◽  
Vol 841 ◽  
pp. 266-272
Author(s):  
Sannasi Abdullah Palsan ◽  
Jia Yi Lim ◽  
Abdullah Nor Asfaliza
Keyword(s):  
Personal Care Products ◽  
Synthesis And Characterization ◽  
Personal Care ◽  
Optimum Conditions ◽  
Color Analysis ◽  
Impregnation Ratio ◽  
Cosmetic Formulation ◽  
Methylene Blue Number ◽  
Highly Porous

Consumer’s preference towards organic material enriched nature based green products in the cosmetics and personal care industry have intensified over the years. This paper shares the synthesis and characterization of an active organic biocarbon material derived from carbonized powdered coconut shell by bicarbonate cured activation for use in charcoal-based cosmetic and personal care products. The optimum conditions for activation was observed at 800 °C, run for 180 min, with a bicarbonate impregnation ratio of 1:3. Experimental design followed Box-Behnken approach. The increase in iodine (757.30 mg/g) and methylene blue number (111.00 mg/g) are indicative of a highly porous biocarbon material that reflects its excellent adsorption capability. Suitability of the biocarbon material for application in charcoal-based cosmetic formulation mixture was supported through proximate, texture, and color analysis. Overall material characteristics are beneficial for effective adsorbent and exfoliant functions in cleansing, detoxifying, and scrubbing.

scholarly journals Resistance Profiling and Molecular Characterization of Extended-Spectrum/Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli Isolated from Healthy Broiler Chickens in South Korea

2020 ◽  
Vol 8 (9) ◽  
pp. 1434 ◽  
Author(s):  
Hyun-Ju Song ◽  
Dong Chan Moon ◽  
Abraham Fikru Mechesso ◽  
Hee Young Kang ◽  
Mi Hyun Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Phylogenetic Analysis ◽  
Resistance Gene ◽  
Broiler Chickens ◽  
Human Consumption ◽  
E Coli ◽  
Extended Spectrum ◽  
First Time ◽  
Pfge Profiles

We aimed to identify and characterize extended-spectrum β-lactamase (ESBL)-and/or plasmid-mediated AmpC β-lactamase (pAmpC)-producing Escherichia coli isolated from healthy broiler chickens slaughtered for human consumption in Korea. A total of 332 E. coli isolates were identified from 339 cloacal swabs in 2019. More than 90% of the isolates were resistant to multiple antimicrobials. ESBL/pAmpC-production was noted in 14% (46/332) of the isolates. Six of the CTX-M-β-lactamase-producing isolates were found to co-harbor at least one plasmid-mediated quinolone resistance gene. We observed the co-existence of blaCMY-2 and mcr-1 genes in the same isolate for the first time in Korea. Phylogenetic analysis demonstrated that the majority of blaCMY-2-carrying isolates belonged to subgroup D. Conjugation confirmed the transferability of blaCTX-M and blaCMY-2 genes, as well as non-β-lactam resistance traits from 60.9% (28/46) of the ESBL/pAmpC-producing isolates to a recipient E. coli J53. The ISECP, IS903, and orf477 elements were detected in the upstream or downstream regions. The blaCTX-M and blaCMY-2 genes mainly belonged to the IncI1, IncHI2, and/or IncFII plasmids. Additionally, the majority of ESBL/pAmpC-producing isolates exhibited heterogeneous PFGE profiles. This study showed that healthy chickens act as reservoirs of ESBL/pAmpC-producing E. coli that can potentially be transmitted to humans.

scholarly journals The Discovery of Twenty-Eight New Encapsulin Sequences, Including Three in Anammox Bacteria

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
John C. Tracey ◽  
Maricela Coronado ◽  
Tobias W. Giessen ◽  
Maggie C. Y. Lau ◽  
Pamela A. Silver ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Phylogenetic Analysis ◽  
Sample Size ◽  
Small Sample Size ◽  
Small Sample ◽  
Anammox Bacteria ◽  
Freshwater Species ◽  
Cargo Protein

AbstractMany prokaryotes encode protein-based encapsulin nanocompartments, including anaerobic ammonium oxidizing (anammox) bacteria. This study expands the list of known anammox encapsulin systems from freshwater species to include the marine genus Scalindua. Two novel systems, identified in “Candidatus Scalindua rubra” and “Candidatus Scalindua sp. SCAELEC01 167” possess different architectures than previously studied freshwater anammox encapsulins. Characterization of the S. rubra encapsulin confirms that it can self-assemble to form compartments when heterologously expressed in Escherichia coli. BLASTp and HMMER searches of additional genomes and metagenomes spanning a range of environments returned 26 additional novel encapsulins, including a freshwater anammox encapsulin identified in “Candidatus Brocadia caroliniensis”. Phylogenetic analysis comparing these 28 new encapsulin sequences and cargo to that of their closest known relatives shows that encapsulins cluster by cargo protein type and therefore likely evolved together. Lastly, prokaryotic encapsulins may be more common and diverse than previously thought. Through searching a small sample size of all public metagenomes and genomes, many new encapsulin systems were unearthed by this study. This suggests that many additional encapsulins likely remain to be discovered.

scholarly journals Morphologycal and Molecular Partial Histone-H3 Characterization of Bintan Sea Snail Gonggong (Strombus sp.) as a Species Validation

2019 ◽  
Vol 26 (2) ◽  
pp. 56
Author(s):  
Lily Viruly ◽  
Nuri Andarwulan ◽  
Maggy T. Suhartono ◽  
Mala Nurilmala
Keyword(s):  
Phylogenetic Analysis ◽  
Genetic Distance ◽  
Molecular Characterization ◽  
Dna Sequences ◽  
Shell Length ◽  
Bioinformatics Analysis ◽  
Economic Value ◽  
Histone H3 ◽  
Shell Width

Sea snail gonggong is an icon of Tanjungpinang-Riau Islands Province. It is a favorite seafood item in Riau Islands Province, and is high economic value but not known widely yet. Until now, sea snail gonggong has been highly exploited but the research on this snail is very limited. The aim of this study was to morphology and molecular characterization of Bintan gonggong snail (Strombus sp.) as a species validation. Bintan gonggong snail included thick-shelled gonggong and thin-shelled gonggong. Morphology identifcation of species Bintan gonggong snail was based on morphometric variability. Molecular identifcation used partial Histone-H3, MEGA version 6.06, and bioinformatics analysis. The result showed that the morphological identifcation of thick-shelled and thin-shelled gonggong based on shell width, the lip thickness, and total weight signifcantly different, but other variables (i.e shell length, shell depth, aperture length, and gonggong weight) were not signifcantly different (p<0.05). Resulted of a molecular identifcation with phylogenetic analysis that the thin-shelled and thick-shelled Bintan gonggong snails were 1 species and a genetic distance of 1%. They were not species Strombus canarium, Strombus vitatus, and Strombus epidromis. Bintan gonggong snails were Strombus turturella (Leavistrombus turturella). DNA sequences of Bintan gonggong have been registered in Gen-Bank with registration numbers MH348131 (thinshelled gonggong) and MH348132 (thick-shelled gonggong).

scholarly journals Characterization of pharmaceuticals, personal care products, and polybrominated diphenyl ethers in lake sturgeon serum and gametes

2020 ◽  
Vol 266 ◽  
pp. 115051
Author(s):  
Jo A. Banda ◽  
Dan Gefell ◽  
Viktoriya An ◽  
Amber Bellamy ◽  
Zy Biesinger ◽  
...  
Keyword(s):  
Polybrominated Diphenyl Ethers ◽  
Personal Care Products ◽  
Lake Sturgeon ◽  
Personal Care ◽  
Diphenyl Ethers

scholarly journals An Investigation into Incidences of Microbial Contamination in Cosmeceuticals in the UAE: Imbalances between Preservation and Microbial Contamination

Cosmetics ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 92
Author(s):  
Ammar Abdulrahman Jairoun ◽  
Sabaa Saleh Al-Hemyari ◽  
Moyad Shahwan ◽  
Sa’ed H. Zyoud
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
United Arab Emirates ◽  
Microbial Contamination ◽  
Personal Care Products ◽  
Good Manufacturing Practice ◽  
Personal Care ◽  
Research Education ◽  
Mesophilic Bacteria

In recent years, concern about certain personal care products and cosmetics suffering from microbial contamination has increased. In this research, we aimed to determine the types and incidence of the most common microorganisms found in unopened/unused personal care and cosmetic products in the United Arab Emirates (UAE) market. This research involved an analysis of 100 personal care products and cosmetics. For every product, microbial (Candida albicans, Staphylococcusaureus, aerobic mesophilic bacteria, Escherichia coli, yeast and mold, and Pseudomonas aeruginosa) contamination was assessed, and levels were compared with the guidelines used in Europe. Of the total samples, 15% (95% CI: 0.79–22.1) were contaminated by aerobic mesophilic bacteria compared to the maximum microbial limit of 1000 CFU/g. In addition, 13% (95% CI: 0.63–19.7) of the samples were contaminated with yeast and mold compared to the maximum microbial limit of 1000 CFU/g. Of all samples, nine (9%) were contaminated with both aerobic mesophilic bacteria and yeast and mold. However, none of the tested samples were contaminated with Escherichia coli, Staphylococcus aureus, Candida albicans, or Pseudomonas aeruginosa. Manufacturers of cosmetics and personal care products should be developing and implementing best practices regarding quality control/quality assurance in partnership with government regulators. Additionally, there should be greater control of the quality and safety of this type of product regarding good manufacturing practice (GMP), regulation, research, education, and the reporting of adverse events.

Sign in / Sign up

Export Citation Format

Share Document