scholarly journals iTRAQ Based Protein Profile Analysis Revealed Key Proteins Involved in Regulation of Drought-Tolerance During Seed Germination in Adzuki Bean

2021 ◽  
Author(s):  
Xuesong Han ◽  
Fangwen Yang ◽  
Yongguo Zhao ◽  
Hongwei Chen ◽  
Zhenghuang Wan ◽  
...  
Keyword(s):  
Seed Germination ◽  
Drought Tolerance ◽  
Profile Analysis ◽  
Protein Profile ◽  
Adzuki Bean ◽  
Bioactive Substances ◽  
Breeding Programs ◽  
Drought Tolerant ◽  
High Quality Protein ◽  
Germinating Seeds

Abstract Adzuki bean is famous as its high-quality protein, fiber, vitamins, minerals as well as rich bioactive substances. However, it is vulnerable to drought at the germination stage. Up to date, little information is available about the genetic controls of drought tolerance during seed germination in adzuki bean. In this study, differential expression proteins（DEPs）were identified based on iTRAQ technology during seed germination between the drought-tolerant variety 17235 and drought-sensitive variety 17033 in adzuki bean. A total of 2834 proteins were identified in the two adzuki bean in the germinating seeds. Eighty-seven and eighty DEPs were increased and decreased accumulation in variety 17235 compared to 17033 under drought, respectively. Meanwhile, 132/205 and 144/123 DEPs were up- or down-regulated in 17235 and 17033 under drought compared to the control, respectively. GO, KEGG, and PPI analysis revealed that the proteins related to carbohydrate metabolism and energy production showed abundantly increase in response to drought stresses. Ectopic overexpression of one candidate encoding V-ATPase in tobacco enhanced the drought tolerance of plants. The results provide valuable insights into adzuki bean response to drought stress, and the DEPs might be applied to develop drought tolerant adzuki bean in breeding programs.

scholarly journals iTRAQ based protein profile analysis revealed key proteins involved in regulation of drought-tolerance during seed germination in Adzuki bean

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xuesong Han ◽  
Fangwen Yang ◽  
Yongguo Zhao ◽  
Hongwei Chen ◽  
Zhenghuang Wan ◽  
...  
Keyword(s):  
Seed Germination ◽  
Drought Tolerance ◽  
Profile Analysis ◽  
Protein Profile ◽  
Control Group ◽  
Adzuki Bean ◽  
Bioactive Substances ◽  
Legume Crop ◽  
Drought Tolerant ◽  
High Quality Protein

AbstractAdzuki bean is an important legume crop due to its high-quality protein, fiber, vitamins, minerals as well as rich bioactive substances. However, it is vulnerable to drought at the germination stage. However, little information is available about the genetic control of drought tolerance during seed germination in adzuki bean. In this study, some differential expression proteins (DEPs) were identified during seed germination between the drought-tolerant variety 17235 and drought-sensitive variety 17033 in adzuki bean using iTRAQ method. A total of 2834 proteins were identified in the germinating seeds of these two adzuki beans. Compared with the variety 17033, 87 and 80 DEPs were increased and decreased accumulation in variety 17235 under drought, respectively. Meanwhile, in the control group, a few DEPs, including 9 up-regulated and 21 down-regulated proteins, were detected in variety 17235, respectively. GO, KEGG, and PPI analysis revealed that the DEPs related to carbohydrate metabolism and energy production were significantly increased in response to drought stresses. To validate the proteomic function, the ectopic overexpression of V-ATPase in tobacco was performed and the result showed that V-ATPase upregulation could enhance the drought tolerance of tobacco. The results provide valuable insights into genetic response to drought stress in adzuki bean, and the DEPs could be applied to develop biomarkers related to drought tolerant in adzuki bean breeding projects.

Phytohormone cross-talk and antioxidant gadgets tussles osmotic stress in indigenous rice landraces during seed germination

2020 ◽  
Author(s):  
R. Anupriya ◽  
Sugitha Thankappan ◽  
A. Senthil ◽  
D. Rajakumar ◽  
Geetha S ◽  
...  
Keyword(s):  
Drought Stress ◽  
Seed Germination ◽  
Drought Tolerance ◽  
Cross Talk ◽  
Tamil Nadu ◽  
Critical Role ◽  
Hydrolytic Enzyme ◽  
Rice Landraces ◽  
Drought Tolerant ◽  
Root And Shoot Length

Abstract Seed germination plays a critical role in determining rice productivity under drought stress. We evaluated 100 traditional rice landraces originated from different agro-ecological zones of Tamil Nadu along with drought- susceptible (IR 64) and drought- tolerant (IR 64 DRT) checks. Moisture stress was induced using PEG 6000 and screening done over a range of osmotic potentials (-) 10 bars, (-) 12.5 bars and (-)15 bars for a period of 5 d. Physio-morphological traits such as germination rate, survival per cent, root and shoot length, vigor index, RS ratio and relative water content (RWC) were assessed during early drought stress. We observed significant changes in the seed macromolecules, phytohormone levels (GA and IAA), osmolytes and antioxidant responses (catalase and superoxide dismutase) between drought stress and control treatments. Kuliyadichan registered significantly higher IAA and GA (44% and 35% respectively over drought tolerant check IR 64 DRT) at drought stress, whereas all the landraces showed an elevated catalase activity. In PC analysis, first three PCs captured 88.93% of the total variation; significant differences were detected among genotypes with respect to the studied parameters. Six traditional landraces such as Kuliyadichan, Rajalakshmi, Sabhagidhan, Nootripathu, Chandaikar and Mallikar were selected and their inherent drought tolerance was associated with metabolic responses viz., triggered hydrolytic enzyme activities, hormonal cross-talk, ROS signaling and catalase under drought stress compared to drought sensitive IR64. Hence, these genotypes can be used as potential donor candidates towards genetic improvement of drought tolerance in rice.

scholarly journals Drought-Tolerant Barley: II. Root Tip characteristics in Emerging Roots

Agronomy ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 220 ◽  
Author(s):  
Andrea Y. Carter ◽  
Michael J. Ottman ◽  
Gilberto Curlango-Rivera ◽  
David A. Huskey ◽  
Brooke A. D’Agostini ◽  
...  
Keyword(s):  
Seed Germination ◽  
Drought Tolerance ◽  
Root Hair ◽  
Underlying Mechanism ◽  
Cell Number ◽  
Root Tip ◽  
Border Cell ◽  
Empirical Survey ◽  
Drought Tolerant ◽  
And Function

Reduced water resources are of increasingly urgent global concern. One potential strategy to address the crisis is the use of drought tolerant crops in agriculture. Barley varieties developed for reduced irrigation (“Solum” and “Solar”) use significantly less water than conventional varieties (“Cochise” and “Kopious”). The underlying mechanism of this drought tolerance is unknown but root structure and function play a key role in plant water uptake. In this study, an empirical survey compared early root development between drought tolerant and conventional varieties. Traits associated with root meristem-regulated cell division including rate of seed germination, border cell number and root cap mucilage production, and root hair emergence were quantified during root emergence. Preliminary results revealed that drought tolerant varieties exhibited faster seed germination and root hair production than conventional varieties. Border cell number and mucilage production in the drought tolerant varieties also were higher than in the conventional variety “Kopious,” but lower than in “Cochise”. Each trait, if found to be linked to the observed drought tolerance, could yield a simple, rapid, and inexpensive tool to screen for new crop varieties. Further detailed studies are needed.

scholarly journals Validation of SSR Molecular Markers Linked to Drought Tolerant in Some Wheat Cultivars

2018 ◽  
Vol 6 (3) ◽  
pp. 95-109
Author(s):  
Manal Eid
Keyword(s):  
Drought Tolerance ◽  
Ssr Markers ◽  
Dna Sequences ◽  
Molecular Descriptors ◽  
Genetic Relationships ◽  
Wheat Cultivars ◽  
Discrimination Power ◽  
Breeding Programs ◽  
Drought Tolerant ◽  
Germplasm Resource

The present study was carried out to conduct drought tolerance in three wheat cultivars including susceptible (Gemmiza7) and tolerant (Sakha93 and Sahel1). Molecular characterization was done by 26 SSR markers located on chromosome7 which was associated with drought tolerance in many previous studies. 26 SSR markers were polymorphic and thus showed 100% polymorphism. The number of alleles per locus varied from 2 to 3 alleles with an average (2.62). The polymorphism information content (PIC) value ranged from 0.34 to 0.59, with a mean of 0.51. The discrimination power (Dp) value ranged between 0.67 and 0.78 with an average of 0.71 per locus and Heterozygosity (He) value varied from 0.44 to 0.67 with an average of 0.59. The genetic relationships estimated by the polymorphism of SSR markers revealed a greater level of genetic variability in wheat cultivars of wide adaptability and applicability. Whereas an average of combined probability value for the SSR markers was 6.15 x 10-16, suggests the capability of the marker system to distinguish identity and purity of wheat cultivars. In addition to the SSR markers revealed various bands that were either absent or present within tolerant cultivars (Sakha93 and Sahel1) which were altogether absent in susceptible cultivar (Gemmiza7). Also, SSRs of diagnostic and curatorial importance were discerned as ‘stand-alone’ molecular descriptors for barcoding the application of DNA sequences of standardized genetic markers for the identification of wheat cultivars. However, the genetic information in this study could provide useful information to address breeding programs and germplasm resource management.

Root-based Responses of Well-watered and Water-stressed Chickpea (Cicer arietinum L.) Genotypes Varying for Drought Tolerance and Biomass 

2021 ◽  
Author(s):  
Hayati Akman
Keyword(s):  
Drought Tolerance ◽  
Cicer Arietinum ◽  
Root Biomass ◽  
Root Traits ◽  
Grain Legume ◽  
Breeding Programs ◽  
Cicer Arietinum L ◽  
Legume Crop ◽  
Drought Tolerant ◽  
Adaptation Mechanisms

Background: Chickpea is a pivotal grain legume crop and is grown in rain-fed conditions where its production has been challenged by drought. Methods: To understand precisely the root-based responses to well-watered (WW) and water-stressed (WS) treatments, 14 chickpea (Cicer arietinum L.) genotypes differing in drought tolerance and biomass were studied in 100-cm cylinders under glasshouse conditions. Result: The genotypes exhibited significant variations in rooting depths ranging from 84.5 to 100.3 cm and 78.7 to 121 cm in WW and WS treatments, respectively and root biomasses varied from 0.23 to 1.01 g and 0.38 to 0.91 g. The average root biomass of drought-tolerant genotypes was 61.3% in WS treatment and 64.4% in WW treatment higher than that of drought-sensitive genotypes. Moreover, genotype with high biomass revealed greater root biomass and deeper rooting than the genotype with low biomass in both treatments. The root biomass in the deeper soil profile differed between drought-tolerant and drought-sensitive genotypes and was generally greater in WS compared to WW treatment. Overall, screening the variability in root features of chickpea genotypes with varying levels of drought tolerance and biomass contributes to new insights for understanding drought adaptation mechanisms and the improvement of new cultivars with superior root traits in breeding programs. 

scholarly journals Toward a New Use for Carbon Isotope Discrimination in Wheat Breeding

Agronomy ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 385
Author(s):  
Dixon ◽  
Carter
Keyword(s):  
Drought Tolerance ◽  
Carbon Isotope ◽  
Water Use ◽  
Carbon Isotope Discrimination ◽  
Wheat Breeding ◽  
Drought Severity ◽  
Deep Roots ◽  
Breeding Programs ◽  
Isotope Discrimination ◽  
Drought Tolerant

A major obstacle in the effort to develop drought tolerant varieties of wheat (Triticum aestivum L.) is phenotyping. Traits known to contribute to improved drought tolerance, such as water-use behavior, reliance on stem reserve carbohydrates, and the ability to develop deep roots, require time and resource-intensive screening techniques. Plant breeding programs often have many thousands of experimental genotypes, which makes testing for each of these traits impractical. This work proposes that carbon isotope discrimination (∆) analysis of mature grains may serve as a relatively high-throughput approach to identify genotypes exhibiting traits associated with drought tolerance. Using ∆ as a proxy for stomatal conductance and photosynthetic capacity, assumptions can be made regarding fundamental plant physiological responses. When combined with knowledge of the terminal drought severity experienced in a particular environment, genotypes exhibiting conservative and rapid water use, deep roots, and reliance on stem reserve carbohydrates may be identified. Preliminary data in support of this idea are presented. Further verification of this use for grain ∆ will better equip wheat breeding programs to develop more drought tolerant varieties.

Genetics of drought tolerance during seed germination in tomato: inheritance and QTL mapping

Genome ◽  
2003 ◽  
Vol 46 (4) ◽  
pp. 536-545 ◽  
Author(s):  
M R Foolad ◽  
L P Zhang ◽  
P Subbiah
Keyword(s):  
Seed Germination ◽  
Drought Tolerance ◽  
Germination Rate ◽  
Rflp Analysis ◽  
Phenotypic Selection ◽  
Allele Frequencies ◽  
Rflp Markers ◽  
Recurrent Parent ◽  
Marker Allele ◽  
Drought Tolerant

A BC1 population (N = 1000) of an F1 hybrid between a stress-sensitive Lycopersicon esculentum breeding line (NC84173; maternal and recurrent parent) and a germination stress-tolerant Lycopersicon pimpinellifolium accession (LA722) was evaluated for seed germination rate under drought stress (DS) (14% w/v polyethyleneglycol-8000, water potential approximately –680 kPa), and the most rapidly germinating seeds (first 3% to germinate) were selected. The 30 selected BC1 seedlings were grown to maturity and self pollinated to produce BC1S1 progeny seeds. Twenty of the 30 selected BC1S1 progeny families were evaluated for germination rate under DS and their average performance was compared with that of a "nonselected" BC1S1 population of the same cross. Results indicated that selection for rapid germination under DS significantly improved progeny germination rate under DS (selection gain = 19.6%), suggesting a realized heritability of 0.47 for rate of germination under DS in this population. The 30 selected BC1 plants were subjected to restriction fragment length polymorphism (RFLP) analysis, and marker allele frequencies for 119 RFLP markers which spanned 1153 cM of the 12 tomato chromosomes were determined. A distributional extreme marker analysis, which measures statistical differences in marker allele frequencies between a selected and a nonselected population, detected four quantitative trait loci (QTLs) for rate of germination under DS in this population. Of these, two QTLs, located on chromosomes 1 and 9, were contributed by the L. pimpinellifolium donor parent and had larger effects than the other two QTLs, located on chromosomes 8 and 12, which were contributed by the L. esculentum recurrent parent. A few BC1S1 families were identified with all or most of the identified QTLs and with germination rates comparable with that of LA722. These families should be useful for the development of germination drought-tolerant tomato lines using marker-assisted selection (MAS). The overall results indicate that drought tolerance during seed germination in tomato is genetically controlled and potentially could be improved by directional phenotypic selection or MAS.Key words: abiotic stress, distributional extreme analysis, heritability, selective genotyping, stress tolerance.

scholarly journals Identification of seed storage protein markers for drought tolerance in mungbean

2016 ◽  
Vol 7 (2) ◽  
Author(s):  
Swapan Kumar Tripathy ◽  
Priyadarshini Mohanty ◽  
Monalisha Jena ◽  
Sasmita Dash ◽  
Devraj Lenka ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Storage Protein ◽  
Vigna Radiata ◽  
Seed Storage Protein ◽  
Protein Profile ◽  
Seed Storage ◽  
Protein Profiling ◽  
Wild Accession ◽  
Drought Tolerant ◽  
Polypeptide Band

<p><strong>A set of 292 mungbean germplasm accessions including 62 popularly adapted local land races and two wild forms (<em>Vigna radiata</em> var. sublobata), important breeding lines and standard ruling varieties were screened for drought stress tolerance at seedling stage.  Eight genotypes e.g., C. No. 35, OUM 14-1, OUM 49-2, Pusa 9072, OM 99-3, Banapur local B, Nipania munga, Kalamunga 1-A) have been identified to possess drought tolerance.  Globulin seed storage protein profiling was carried out in 19 selected mungbean genotypes comprising eight drought tolerant, seven drought sensitive, two wild forms of mungbean (TCR 20 and TCR 213) and two standard checks (LGG 460 and T 2-1</strong><strong>)</strong><strong> to explore differentially expressed polypeptides. Seed protein profiles revealed 15 scorable polypeptide bands with molecular weights ranging from 10.0 to 102.2kD. A specific 12.8kD polypeptide band was present in all above drought tolerant test genotypes including the wild accession TCR 20. Such a polypeptide band may serve as useful biochemical marker for identification of drought tolerant genotypes in mungbean. </strong></p><p><strong>            Key words: </strong>Genetic diversity, seed storage protein profile, wild and cultivated <em>Vigna radiata.</em><strong> </strong></p>

Evaluation of drought tolerance and screening for drought-tolerant indicators in sweetpotato cultivars

2019 ◽  
Vol 45 (3) ◽  
pp. 419 ◽  
Author(s):  
Hai-Yan ZHANG ◽  
Bei-Tao XIE ◽  
Bao-Qing WANG ◽  
Shun-Xu DONG ◽  
Wen-Xue DUAN ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Drought Tolerant

scholarly journals Exploring Smallholder Farmers’ Preferences for Climate-Smart Seed Innovations: Empirical Evidence from Southern Ethiopia

2021 ◽  
Vol 13 (5) ◽  
pp. 2786
Author(s):  
Shimelis Araya Geda ◽  
Rainer Kühl
Keyword(s):  
Smallholder Farmers ◽  
Preference Heterogeneity ◽  
Southern Ethiopia ◽  
Significant Preference ◽  
Experiment Data ◽  
Breeding Programs ◽  
Drought Tolerant ◽  
The Face ◽  
Mnl Model ◽  
Participatory Research Methods

Rapid plant breeding is essential to overcome low productivity problems in the face of climatic challenges. Despite considerable efforts to improve breeding practices in Ethiopia, increasing varietal release does not necessarily imply that farmers have access to innovative varietal choices. Prior research did not adequately address whether varietal attributes are compatible with farmers’ preferences in harsh environmental conditions. With an agricultural policy mainly aiming to achieve productivity maximization, existing breeding programs prioritize varietal development based on yield superiority. Against this background, we estimated a multinomial logit (MNL) model based on choice-experiment data from 167 bean growers in southern Ethiopia to explore whether farmers’ attribute preferences significantly diverge from those of breeders’ priorities. Four important bean attributes identified through participatory research methods were used. The results demonstrate that farmers have a higher propensity toward drought-tolerant capability than any of the attributes considered. The model estimates further show the existence of significant preference heterogeneity across farmers. These findings provide important insight to design breeding profiles compatible with specific producer segments. We suggest demand-driven breeding innovations and dissemination strategies in order to accelerate the adoption of climate-smart and higher-yielding bean innovations that contribute to achieve the national and global sustainability goals in Ethiopia.

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