scholarly journals Evaluation of the Genotoxicity in Fish Erythrocytes to Diagnose the Water Quality of Two Amazonian Estuaries Using the Micronucleus Test and Comet Assay

2021 ◽  
Author(s):  
CLAUDIA ANTONIA CAMPOS RODRIGUES DE OLIVEIRA ◽  
Paulo Sergio dos Santos Souto ◽  
Dulcideia da Conceição Palheta ◽  
Marcelo de Oliveira Bahia ◽  
Lorena Araújo da Cunha ◽  
...  
Keyword(s):  
Comet Assay ◽  
Micronucleus Test ◽  
Genetic Material ◽  
Aquatic Organisms ◽  
Brazilian Amazon ◽  
Genotoxic Effects ◽  
Blood Samples ◽  
Genomic Damage ◽  
And Control

Abstract Genotoxicity studies in coastal ecosystems have been a priority in Environmental Risk Assessment (ERA). This research aimed to study the genotoxicity by the micronucleus test and comet assay in two Brazilian Amazon estuaries (anthropized and control) using Plagioscion squamosissimus as a bioindicator. Blood samples were collected from 54 specimens. No significant genotoxic effects were detected in the cells analyzed, although the highest occurrence was observed in anthropized site. The percentage of genomic damage differed between the sites studied, being always higher in anthropizes site as well. Of the nucleoids analyzed in this site, on average 28±14.42% of the cells were classified in the highest damage class (4). The fish analyzed in the present study are direct influence of xenobiont agents capable of producing damage to the genetic material of aquatic organisms in both sites and, consequently, may bring consequences still little reported in studies of morphophysiological alterations in humans.

scholarly journals Evaluation of the genotoxicity in fish erythrocytes to diagnose the water quality of two Amazonian estuaries using the micronucleus test and comet assay

2021 ◽  
Author(s):  
CLAUDIA ANTONIA CAMPOS RODRIGUES DE OLIVEIRA ◽  
Paulo Sergio dos Santos Souto ◽  
Dulcideia da Conceição Palheta ◽  
Marcelo de Oliveira Bahia ◽  
Lorena Araújo da Cunha ◽  
...  
Keyword(s):  
Comet Assay ◽  
Micronucleus Test ◽  
Genetic Material ◽  
Aquatic Organisms ◽  
Brazilian Amazon ◽  
Genotoxic Effects ◽  
Blood Samples ◽  
Genomic Damage ◽  
And Control

Abstract Genotoxicity studies in coastal ecosystems have been a priority in Environmental Risk Assessment (ERA). This research aimed to study the genotoxicity by the micronucleus test and comet assay in two Brazilian Amazon estuaries (anthropized and control) using Plagioscion squamosissimus as a bioindicator. Blood samples were collected from 54 specimens. No significant genotoxic effects were detected in the cells analyzed, although the highest occurrence was observed in anthropized site. The percentage of genomic damage differed between the sites studied, being always higher in anthropizes site as well. Of the nucleoids analyzed in this site, on average 28 ± 14.42% of the cells were classified in the highest damage class (4). The fish analyzed in the present study are direct influence of xenobiont agents capable of producing damage to the genetic material of aquatic organisms in both sites and, consequently, may bring consequences still little reported in studies of morphophysiological alterations in humans.

scholarly journals Genotoxic Effect in Autoimmune Diseases Evaluated by the Micronucleus Test Assay: Our Experience and Literature Review

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Olivia Torres-Bugarín ◽  
Nicole Macriz Romero ◽  
María Luisa Ramos Ibarra ◽  
Aurelio Flores-García ◽  
Penélope Valdez Aburto ◽  
...  
Keyword(s):  
Autoimmune Diseases ◽  
Micronucleus Test ◽  
Genetic Material ◽  
Genotoxic Effect ◽  
Invasive Test ◽  
Genome Damage ◽  
Organ Specific ◽  
Genomic Damage

Autoimmune diseases (AD) are classified into organ-specific, systemic, and mixed; all forms of AD share a high risk for cancer development. In AD a destructive immune response induced by autoreactive lymphocytes is started and continues with the production of autoantibodies against different targets; furthermore apoptosis failure and loss of balance in oxidative stress as a consequence of local or systemic inflammation are common features seen in AD as well. Micronucleus (MN) assay can be performed in order to evaluate loss of genetic material in a clear, accurate, fast, simple, and minimally invasive test. The MN formation in the cytoplasm of cells that have undergone proliferation is a consequence of DNA fragmentation during mitosis and the appearance of small additional nuclei during interphase. The MN test, widely accepted forin vitroandin vivogenotoxicity research, provides a sensitive marker of genomic damage associated to diverse conditions. In here, we present a review of our work and other published papers concerning genotoxic effect in AD, identified by means of the MN assay, with the aim of proposing this tool as a possible early biomarker for genotoxic damage, which is a consequence of disease progression. Additionally this biomarker could be used for follow-up, to asses genome damage associated to therapies.

Nicotine derived genotoxic effects in human primary parotid gland cells as assessed in vitro by comet assay, cytokinesis-block micronucleus test and chromosome aberrations test

2014 ◽  
Vol 28 (5) ◽  
pp. 838-846 ◽  
Author(s):  
Christian Ginzkey ◽  
Gudrun Steussloff ◽  
Christian Koehler ◽  
Marc Burghartz ◽  
Agmal Scherzed ◽  
...  
Keyword(s):  
Comet Assay ◽  
Parotid Gland ◽  
Chromosome Aberrations ◽  
Micronucleus Test ◽  
Genotoxic Effects ◽  
Gland Cells

Genotoxic effects of the fungicide thiophanate-methyl on Podarcis sicula assessed by micronucleus test, comet assay and chromosome analysis

Ecotoxicology ◽  
2011 ◽  
Vol 20 (4) ◽  
pp. 885-891 ◽  
Author(s):  
T. Capriglione ◽  
S. De Iorio ◽  
F. Gay ◽  
A. Capaldo ◽  
M. C. Vaccaro ◽  
...  
Keyword(s):  
Comet Assay ◽  
Micronucleus Test ◽  
Chromosome Analysis ◽  
Genotoxic Effects ◽  
Podarcis Sicula ◽  
Thiophanate Methyl

scholarly journals New approaches to the assessment of the quality of genetic material bulls

2017 ◽  
Vol 19 (74) ◽  
pp. 99-102
Author(s):  
M. Savelieva ◽  
A. Suchko
Keyword(s):  
Liquid Nitrogen ◽  
Genetic Material ◽  
Freezing And Thawing ◽  
Control Groups ◽  
The Real ◽  
Second Stage ◽  
Cellular Debris ◽  
Physiological Indexes ◽  
And Control

On  the first stage  experiments  are conducted  by the aim of establishment  on  how  many higher the real indexes of fresh sperm and dilute protective environments after moving away of cellular debris, extraneous cages and dead cages. On the second stage, comparison of basic physiological indexes of the fertilization faction got from deconservation sperm and compared to the corresponding indexes of з sperm was conducted, that was not subject to the special cleansing procedure. Difference  between research and control groups, in relation to survivability of sperm and index of absolute survivability of sperm was statistically reliable (Р < 0,001), as for fresh sperm so for sperm, that carried freezing and  thawing in liquid nitrogen. Faction of sperm, that has impregnating ability potentially, as fresh sperm so that was subject to freezing and thawing substantially differ in the row of biological indexes of sperm,  that creates  the necessity  of introduction of new additional descriptions for the analysis of sperm. Index  of  survivability  of  sperm, that potentially has impregnating ability at  deconservation  sperm rose on three hours or on a 32,8 percent’s . Index of absolute survivability of sperm, that potentially has impregnating ability rose on 8,19 conditional units. The modernized method of estimation of quality of sperm of bulls is offered. It is  set that  treatment of sperm with the  use of gradients of closeness exceeds the indexes of activity, to  survivability and absolute survivability as at  fresh standards of sperm so at  deconservation standards of sperm, that gives an opportunity more objectively  to describe quality of sperm. Results over of researches of biological quality of  sperm of bulls of meat blackly-pied and red-pied suckling breed are brought depending on the method of treatment of tests. Additional  descriptions of analysis sperm are entered, they can become the major criteria of estimation of full value and impregnating ability.

scholarly journals Dental curing light: non-ionizing electromagnetic radiation and its possible genotoxic effects

2020 ◽  
Vol 9 (10) ◽  
pp. e4609108884
Author(s):  
Michele de Sampaio Sousa ◽  
Laryssa Roque da Silva ◽  
Danniel Cabral Leão Ferreira ◽  
Fabrício Pires Moura do Amaral ◽  
Rosemarie Brandim Marques ◽  
...  
Keyword(s):  
Comet Assay ◽  
Rattus Norvegicus ◽  
Micronucleus Test ◽  
Clinical Care ◽  
Positive Control ◽  
Distilled Water ◽  
Test Results ◽  
Control Groups ◽  
Genotoxic Effects ◽  
Curing Light

Objective: In this scientific study, we aimed to evaluate genotoxic effects in rats (Rattus norvegicus), related to different periods of exposures to the LED curing light. Methodology: For the genotoxicity evaluation, the rats received lights from the LED photopolymerizer for 40 sec, 10 min and 7-and-a-half minutes, while the negative and positive control groups were treated with distilled water and cyclophosphamide by intraperitoneal, respectively. A sample of peripheral blood was collected from the animals for the comet assay. The bone marrow was collected from each rat for the micronucleus test. Results: It was observed that in the comet assay and micronucleus test, the animals exposed to LED for 10 min, showed genotoxic damage, and they have not presented toxicity degree in the periods of 40 sec and 7-and-a-half minutes. Conclusion: It is possible to conclude that, there was genotoxic effects on the animals' teeth when exposed to the LED curing light in 10 min. However, in the periods of 40 sec, and 7- and-a-half minutes, have been not observed genotoxic effects. This means these times are safe for professional dentists in clinical care.

Enhancement of genotoxic effects in the comet assay with human blood samples by aphidicolin

2004 ◽  
Vol 153 (3) ◽  
pp. 303-310 ◽  
Author(s):  
Günter Speit ◽  
Petra Schütz ◽  
Heike Hoffmann
Keyword(s):  
Comet Assay ◽  
Human Blood ◽  
Genotoxic Effects ◽  
Blood Samples ◽  
Human Blood Samples

scholarly journals Comet assay as a method used in research on DNA damage caused by pesticides in fish

2021 ◽  
Vol 77 (01) ◽  
pp. 6482-2021
Author(s):  
Księżarczyk M. ◽  
Leśniak P. ◽  
Arciszewski M. B. ◽  
Valverde Piedra J. L.
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Research Method ◽  
Genetic Material ◽  
Aquatic Organisms ◽  
Assay Method ◽  
Negative Effects ◽  
Chemical Agents ◽  
Harmful Substances ◽  
Cellular Dna

The comet assay method is a research technique for detecting damage to cellular DNA due to active physical or chemical agents. The comet assay is based on electrophoretic migration of genetic material contained in the cell’s nucleus. This research method is commonly used in many different fields, such as toxicology, environmental protection, and pharmacology. In recent years, the comet assay has attracted considerable attention from scientists studying the effects of harmful substances on the genetic material in the cell’s nucleus. The presence of pesticides in the environment is a threat to animals, because of the negative effects of pesticides on cells and their genetic material. Therefore, the aim of this paper, based on the available literature, was to describe the use of the comet assay in assessing the genotoxicity of pesticides to cells of aquatic organisms, as well as to describe the methodology and potential complications of this procedure.

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