scholarly journals Anti-Diabetic, Anti-Inflammatory and Antioxidant Properties of Four Underutilized Ethnomedicinal Plants: An in Vitro Approach

2020 ◽  
Author(s):  
Sudeshna Datta ◽  
Tapan Seal
Keyword(s):  
Phenolic Content ◽  
Protein Denaturation ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Chelating Activity ◽  
Metal Chelating ◽  
Anti Inflammatory

Abstract Background : In the present study, antidiabetic, anti-inflammatory activities and antioxidant properties in four diverse dissolvable concentrates of four lesser known ethnomedicinal plants viz. Apluda mutica, Mikania micrantha, Kyllinga nemoralis and Cleome rutidosperma were investigated. Methods: The benzene, chloroform, methanol and 70% aqueous (aq.) ethanol extract of these plants were tried for antioxidant by various established in vitro systems like total phenolic content, DPPH free radical scavenging, ABTS radical cation scavenging, metal chelating activity. Anti-diabetic and anti-inflammatory activities were explored by quantifying α-amylase, α-glucosidase and protein denaturation inhibitory activities of the investigated plants. Results: Among the various solvents 70% aq. ethanol extract of M. micrantha had the highest total phenolic content (230.450 ± 0.12mg GAE/g extract), DPPH & ABTS radical scavenging activity, FRAP value (4.122± 0.004 μM Trolox equivalent/ g dry extract), anti-lipid peroxidation capacity, reducing power and metal chelating activity. The highest amount of total flavonoid was detected in the 70% aq. ethanol extract of C. rutidosprema (71.050 ± 0.058 mg RE /g extract. Similarly M. micrantha also exhibited significantly lower IC 50 values for the percentage inhibition of α-amylase (IC50 58.44 ± 0.012 µg/ml) and α-glucosidase (IC50 113.31 ± 0.010 µg/ml) compared to acarbose (IC50 = 53.8 ± 0.009 µg/ml ; IC50 = 79.48 ± 0.006 µg/ml respectively) (p ≤ 0.05). Anti-inflammatory activity was determined by using protein denaturation assay and M. micrantha showed significant lower IC50 value for the inhibition of protein denaturation (IC50= 89.27 ± 0.008 µg/ml) compared to other plants under investigation. Quantification of polyphenolics by HPLC showed the presence of different phenolic acids in varying amounts. Conclusion: Therefore, the results indicate that these plants were shown to contain a remarkable amount of different bio-active compounds, thus confirming their involvement in several biological activities and to serve as a potential antioxidative, anti-inflammatory and anti-diabetic agent in food and pharmaceutical industries.

scholarly journals Evaluation of the In Vitro Wound-Healing Activity and Phytochemical Characterization of Propolis and Honey

2020 ◽  
Vol 10 (5) ◽  
pp. 1845 ◽  
Author(s):  
Alexandra M. Afonso ◽  
Joana Gonçalves ◽  
Ângelo Luís ◽  
Eugenia Gallardo ◽  
Ana Paula Duarte
Keyword(s):  
Wound Healing ◽  
Phenolic Compounds ◽  
Protein Denaturation ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Natural Substances ◽  
Anti Inflammatory ◽  
Free Radical Scavenging Assay ◽  
Wound Healing Activity

Honey and propolis are natural substances produced by Apis mellifera that contain flavonoids, phenolic acids, and several other phytochemicals. The aim of this study was to phytochemically characterize three different types of honey and propolis, both separately and mixed, and to evaluate their wound-healing activity. Total phenolic compounds and flavonoids were determined using the Folin–Ciocalteu’s and aluminum chloride colorimetric methods, respectively. The antioxidant activity was evaluated by both the DPPH free radical scavenging assay and β-carotene bleaching test, and the anti-inflammatory activity was determined by a protein denaturation method. To evaluate the wound-healing activity of the samples, NHDF cells were subjected to a wound scratch assay. The obtained results showed that dark-brown honey presents a higher concentration of phenolic compounds and flavonoids, as well as higher antioxidant and anti-inflammatory activities. Propolis samples had the highest concentrations in bioactive compounds. Examining the microscopic images, it was possible to verify that the samples promote cell migration, demonstrating the wound-healing potential of honey and propolis.

scholarly journals Phytochemical Screening and In Vitro Antioxidant and Anti-inflammatory Activities of Aerial Parts of Euphorbia hirta L.

2021 ◽  
Vol 42 (1) ◽  
pp. 115-124
Author(s):  
Deepak Basyal ◽  
Astha Neupane ◽  
Durga Prasad Pandey ◽  
Shiva Pandeya
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Euphorbia Hirta ◽  
Anti Inflammatory

Euphorbia hirta L (Euphorbiaceae) also called asthma herb has long been prescribed in traditional medicine because it exhibits diverse pharmacological actions due to the presence of alkaloids, flavonoids, polyphenols, triterpenoids, and saponins. The present study is aimed at the study of phytochemical and antioxidant activity and anti-inflammatory screening of E. hirta. Extraction of dried powder was performed followed by phytochemical screening using color reactions. Total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were estimated by Folin-Ciocalteu and Aluminum chloride method respectively. The antioxidant activity was studied by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method.  Anti-inflammatory activity was studied by using protein denaturation in vitro bioassay. Phytochemical screening showed the presence of flavonoids, alkaloids, and phenolic compounds. TPC, TFC and antioxidant activity (IC50) of the extract were found as 288.10 mg gallic acid equivalent per gram (GAE/g), 29.36 mg quercetin equivalent per gram (QE/g) and 32.23 µg/mL (p<0.05) respectively. Diclofenac sodium and E. hirta extract showed the maximum inhibition of 91.28% and 68.20% respectively at the concentration of 1000 µg/mL compared with control (p>0.05). The phenolic compounds and flavonoids exert antioxidant and anti-inflammatory activities because of their scavenging ability. The demonstrated antioxidant and anti-inflammatory activities may be the rationale behind some of its folkloric uses and also may be responsible for some of its pharmacological effects. Thus, E. hirta can be considered a good source of antioxidants and anti-inflammatory actions, which might be beneficial for combating oxidative stress.

scholarly journals In vitro evaluation of the antioxidant, anti-Propioni bacterium acne and antityrosinase effects of Equisetum ramosissimum (Jordanian horsetail)

2020 ◽  
Vol 19 (10) ◽  
pp. 2147-2152
Author(s):  
Noor Sabah Abed Savaya ◽  
Reem Adnan Issa ◽  
Wamidh Hadi Talib
Keyword(s):  
Gallic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Screening Tests ◽  
Total Phenolic ◽  
Aqueous Methanol

Purpose: The investigation of Equisetum ramosissimum Desf. (Equisetaceae) extracts for total phenolic content, potential antioxidant properties, and antibacterial activity against Propioni bacterium acne and antityrosinase effects.Methods: The aerial parts of the E. ramosissimum from Jordan were extracted by maceration and Soxhlet methods, using solvents of different polarities. The composition of the extracts were qualitatively screened using standard phytochemical tests. Quantitatively, total phenolic content (mg/mL, equivalent to gallic acid), ABTS free radical scavenging activity (IC50), anti-P. acne (MIC and MBC), and tyrosinase inhibitory effects (IC50) were also determined.Results: The aqueous-methanol Soxhlet extract contained the highest total phenolic content (0.675 mg/mL gallic acid equivalents). Besides, phytochemical screening tests revealed the presence of phenols, flavonoids, tannins, alkaloids and saponins in the aqueous methanol Soxhlet extract, contributing to the antioxidant (IC50 = 0.125) and antityrosinase (IC50 = 1.125) effects. This extract also showed potent antimicrobial effects against P. acne (MIC = 6.250 mg/mL; MBC = 12.500 mg/mL). Other extracts, including ethanol, water and ethyl acetate, showed lower total phenolic content with moderate and weak biological activity.Conclusion: E. ramosissimum is a promising plant species to be considered for antioxidant, antiacne, and antityrosinase effects. However, further testing (including invivo, histological examination, and highperformance liquid chromatography (HPLC) analysis) is necessary to understand more about its mechanisms of action. Keywords: Antioxidant, Anti-Propioni bacterium acne, Antityrosinase, Equisetum ramosissimum, Total phenolic content, Jordanian horsetail

scholarly journals In-vitro Antioxidant and Anti-inflammatory Activities of Quisqualis indica Linn. Leaves Extract

2019 ◽  
pp. 1-13
Author(s):  
Pallavi Pal ◽  
Ajeet Singh
Keyword(s):  
Protein Denaturation ◽  
Radical Scavenging Activity ◽  
Diclofenac Sodium ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Standard Drug ◽  
Anti Inflammatory

Aim: In this study antioxidant and anti-inflammatory effect of ethanolic extract of Quisqualis indica leaves was evaluated. Study Design: In-vitro analysis of Quisqualis indica leaf extract. Place and Duration of Study: Molecular Biology laboratory, Department of Biotechnology, G.B Pant Engineering College, Pauri, between July 2015 and July 2016. Methods: Non-enzymatic and enzymatic assays such as DPPH (1, 1diphenyl-2-picryl hydrazyl), FRAP assay, superoxide dismutase SOD (EC 1.15.1.1), catalase (EC 1.11.1.6), for radical scavenging activity of ethanolic extracts of Quisqualis indica Linn. plant leaves had done. For estimation of anti-inflammatory action, two methods were employed: protein denaturation method and membrane stabilization method. Results: Ethanolic extract of leaves on higher concentration had better antioxidant potential when compared with reference standard ascorbic acid. They exhibited strong antioxidant radical scavenging activity values for ethanolic extract of leaves. Results of anti-inflammatory method suggested better potential values for ethanolic extract and compared with standard drug diclofenac sodium respectively. A significant relationship between antioxidant, anti-inflammatory capacity and total phenolic content was examined, indicating that phenolic compounds are the major contributors for the antioxidant and anti-inflammatory properties of this plant. Conclusion: Ethanolic extract of Q. indica exhibited strong anti-inflammatory and antioxidant activity and this can be used for designing novel drug inhibitors with better efficacy.

Antioxidant properties and consumer acceptability of pearl millet – tiger nut biscuits

2015 ◽  
Vol 45 (6) ◽  
pp. 818-828 ◽  
Author(s):  
Olufunmilayo Sade Omoba ◽  
Oluwadamilola Olamiiposi Dada ◽  
Sule Ola Salawu
Keyword(s):  
Pearl Millet ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Consumer Acceptability ◽  
Potential Health ◽  
Content Type ◽  
Flour Blends

Purpose – This paper aims to focus on the antioxidant properties and consumer acceptability of biscuits produced from pearl millet (PM)–tiger nut (TN) composite blends, with the aim of encouraging the use of these under-utilised food crops in producing value-added products with nutraceutical potential. Design/methodology/approach – PM grains and TN seeds were processed into flour and blended at ratios: 15:85 (A), 20:80 (B), 25:75 (C), 30:70 (D), 35:65 (E), 40:60 (F), 45:55 (G), of PM to TN using response surface methodology. The antioxidant properties of the biscuits produced from the flour blends were determined using standard methods, and data obtained were subjected to analysis of variance (ANOVA); differences of means were separated using Duncan multiple range test. The biscuits were subjected to sensory evaluation using 50 untrained panellist and results statistically analysed. Findings – The total phenolic content (TPC-mg/GAE) of PM–TN flour blends ranged from 2.65-4.95 (A-G), with an average TPC of 3.85. In PM–TN biscuits, total phenolic values ranged from 1.20-3.42 (A-G), with an average of 2.39. The 2,2′-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activities (μmolTEAC/g) of PM–TN flour blends ranged from 26.7-36.4 (A-G), and the values for biscuits ranged from 28.5-38.6 (A-G). There was a highly significant correlation, r = 0.811(p < 0.001) and r = 0.913 (p < 0.001) between phenolic content and antioxidant activity for flour and biscuit, respectively. The effect of in vitro digestion of biscuits on the antioxidant assays viz-a-viz: TPC and ABTS radical scavenging activities were also investigated. The order of phenolic release was enzymic > aqueous > ethanolic digest for TPC, while for ABTS, the order of release was enzymic > ethanolic > aqueous. The highest values for antioxidant properties were found in the simulated in vitro enzyme digested biscuits. Consumer acceptability revealed that the overall acceptability scores of the biscuits were not significantly different except for biscuit sample G. Originality/value – The paper has demonstrated the antioxidant potentials and consumer acceptability of PM–TN biscuits. It also shows the effect of in vitro digestion on the antioxidant activities of the biscuit and its potential health benefit, as a source of antioxidant.

scholarly journals Phenolic content and antioxidant activity of Echinocystis lobata (Mich.) Torr. ET Gray (Cucurbitaceae)

10.5219/1579 ◽  
2021 ◽  
Vol 15 ◽  
pp. 784-791
Author(s):  
Yulia Vinogradova ◽  
Olga Shelepova ◽  
Olena Vergun ◽  
Olga Grygorieva ◽  
Jan Brindza
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Echinocystis Lobata

Echinocystis lobata (Mich.) Torr. et Gray extracts were investigated for their bioactive compounds and their potential for natural antioxidant properties. In vitro antioxidant activity of methanol, ethanol, and water extracts were evaluated by 1,1-diphenyl-picrylhydrazyl (DPPH) radical scavenging activity. Total phenolic content (TPC) and total flavonoid content (TFC) of fruits and seeds were determined. TPC is a fairly constant value and varies weakly both among the different organs of Echinocystis lobata and among the geographical locations of the populations studied. Like other invasive species, Echinocystis lobata is superior to many other plants in terms of the total amount of flavonoids contained in its fruits and seeds. The dried fruits contain more flavonoids than the seeds. Seeds from Slovak populations have higher flavonoid content than those from Russian populations. The results demonstrated that Echinocystis lobata can be a good source of natural antioxidants and have a great potential for commercialization, especially in the pharmaceutical industry.

scholarly journals Effects of Long-Term Storage on Radical Scavenging Properties and Phenolic Content of Kombucha from Black Tea

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5474 ◽  
Author(s):  
Chiara La Torre ◽  
Alessia Fazio ◽  
Paolino Caputo ◽  
Pierluigi Plastina ◽  
Maria Cristina Caroleo ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Ph Value ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Black Tea ◽  
In Vitro Models ◽  
Total Phenolic ◽  
Organoleptic Characteristics ◽  
Radical Scavenging Properties

Kombucha is a fermented beverage. Its consumption has significantly increased during the last decades due to its perceived beneficial effects. For this reason, it has become a highly commercialized drink that is produced industrially. However, kombucha is still also a homemade beverage, and the parameters which, besides its organoleptic characteristics, define the duration of its potential beneficial properties over time, are poorly known. Therefore, this study aimed to determine the effect of 9-month storage at 4 °C with 30-day sampling on the pH, total phenolic, and flavonoid contents, free radical scavenging properties of kombucha fermented from black tea. Our results highlighted that, after four months, the phenolic content decreased significantly from the initial value of 234.1 ± 1.4 µg GAE mL−1 to 202.9 ± 2.1 µg GAE mL−1, as well its antioxidant capacity tested by two in vitro models, DPPH, and ABTS assays. Concomitantly, the pH value increased from 2.82 to 3.16. The novel findings of this pilot study revealed that kombucha from sugared black tea can be stored at refrigerator temperature for four months. After this period the antioxidant properties of kombucha are no longer retained.

scholarly journals IN VITRO EVALUATION OF ANTIOXIDANT POTENTIAL AND TOTAL PHENOLIC CONTENT OF BARLERIA LONGIFLORA LEAF EXTRACTS

2019 ◽  
pp. 212-215
Author(s):  
JOTHIMUNIYANDI M ◽  
JAYACHITRA A
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Metal Chelating ◽  
Reducing Ability

Objective: The aim of this present study is to estimate the antioxidant potential found in the leaves of Barleria longiflora that belongs to the family Acanthacea. Methods: Antioxidant activity of six different solvent extracts system of B. longiflora leaves was assayed for 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical activity, scavenging of hydrogen peroxide (H2O2), ferric reducing antioxidant activity, metal chelating ability assay, and total reducing ability. Total phenolic content of the extracts was determined by Folin–Ciocalteu method. Results: The radical scavenging activity was evaluated by DPPH of ethanol extract at concentration of 100 μg/mg was found to be 56.5% followed by methanol 48.4%, whereas maximum scavenging of H2O2 was observed in ethanol 83.4% followed by chloroform 70.8%. Ethanolic extract of B. longiflora leaves showed the highest value in ferric reducing antioxidant power assay 74.8%, metal chelating activity 61.6%, and total reducing ability 0.76±0.02 when compared to the standard ascorbic acid. Conclusion: The results suggest that the antioxidant potential of the ethanol extract has the highest activity in compared to other five extracts of B. longiflora leaves.

scholarly journals Comparison of Phenolic Metabolites in Purified Extracts of Three Wild-Growing Herniaria L. Species and Their Antioxidant and Anti-Inflammatory Activities In Vitro

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 530
Author(s):  
Solomiia Kozachok ◽  
Joanna Kolodziejczyk-Czepas ◽  
Svitlana Marchyshyn ◽  
Krzysztof Kamil Wojtanowski ◽  
Grażyna Zgórka ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Mononuclear Cells ◽  
Phenolic Content ◽  
Interleukin 2 ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Human Blood Plasma ◽  
Inhibitory Effects ◽  
Anti Inflammatory

The work is aimed at phytochemical characterization and In Vitro evaluation of antioxidant actions, anti-inflammatory effects, and cytotoxicity of purified extracts from three rupturewort (Herniaria L.) species, i.e., Herniaria glabra (HG), H. polygama (HP), and H. incana herb (HIh). The total phenolic content established in the purified extracts (PEs) of HIh, HP, and HG was 29.6, 24.0, and 13.0%, respectively. Thirty-eight non-saponin metabolites were identified using LC-HR-QTOF-ESI-MS; however, only 9 were common for the studied Herniaria species. The most abundant phenolic compound in HG-PE was narcissin (7.4%), HP-PE shared 3 major constituents, namely cis-2-hydroxy-4-methoxycinnamic acid 2-O-β-glucoside (cis-GMCA, 5.8%), narcissin (5.4%), and rutin (5.3%). Almost half of HIh phenolic content (14.7%) belonged to oxytroflavoside A (7-O-methylkaempferol-3-O-[3-hydroxy-3-methylglutaryl-(1→6)]-[α-rhamnopyranosyl-(1®2)]-β-galactopyranoside). Antioxidant properties of the Herniaria PEs were evaluated employing an experimental model of human blood plasma, exposed to the peroxynitrite-induced oxidative stress. The assays demonstrated significant reduction of oxidative damage to protein and lipid plasma components (estimated by measurements of 3-nitrotyrosine, protein thiol groups, thiobarbituric acid-reactive substances), and moderate protection of its non-enzymatic antioxidant capacity. Anti-inflammatory properties of the Herniaria PEs were evaluated In Vitro as inhibitory effects against cyclooxygenases (COX-1 and -2) and concanavalin A-induced inflammatory response of the peripheral blood mononuclear cells (PBMCs). None of the studied plants showed inhibitory effects on COXs but all purified extracts partly reduced the release of interleukin 2 (IL-2) and tumor necrosis factor-alpha (TNF-α) from PBMCs, which suggested their prospective ability to up-regulate inflammatory response of the cells. The purified extract from H. glabra turned out to be the most efficient suppressor of PBMCs’ inflammatory response. Additionally, cytotoxicity of purified Herniaria extracts on PBMCs was ruled out based on In Vitro studies.

scholarly journals Hepatoprotective studies on methanolic extract fractions of Lindernia ciliata and development of qualitative analytical profile for the bioactive extract

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Praneetha Pallerla ◽  
Narsimha Reddy Yellu ◽  
Ravi Kumar Bobbala
Keyword(s):  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Liver Homogenate ◽  
Reducing Power ◽  
Hepatoprotective Activity ◽  
Total Phenolic ◽  
Effective Fraction

Abstract Background The objective of the study is to evaluate the hepatoprotective activity of methanolic extract fractions of Lindernia ciliata (LC) and development of qualitative analytical profile of the bioactive fraction using HPLC fingerprinting analysis. All the fractions of methanolic extract of Lindernia ciliata (LCME) are assessed for their total phenolic, flavonoid contents and in vitro antioxidant properties by using DPPH, superoxide, nitric oxide, hydroxyl radical scavenging activities and reducing power assay. Acute toxicity study was conducted for all the fractions and the two test doses 50 and 100 mg/kg were selected for the hepatoprotective study. Liver damage was induced in different groups of rats by administering 3 g/kg.b.w.p.o. paracetamol and the effect of fractions were tested for hepatoprotective potential by evaluating serum biochemical parameters and histology of liver of rats. The effective fraction was evaluated for its antihepatotoxic activity against D-Galactosamine (400 mg/kg b.w. i.p.) and in vivo antioxidant parameters viz., Glutathione (GSH), Melondialdehyde (MDA) and Catalase (CAT) levels are estimated using liver homogenate. Results Among all the fractions, butanone fraction of LCME, (BNF-LCME) has shown better hepatoprotective activity and hence it is selected to evaluate the antihepatotoxicity against D-GaIN. The activity of BNF-LCME is well supported in in vitro and in vivo antioxidant studies and may be attributed to flavonoidal, phenolic compounds present in the fraction. Hence, BNF-LCME was subjected to the development of qualitative analytical profile using HPLC finger printing analysis. Conclusions All the fractions of LCME exhibited significant hepatoprotective activity and BNF-LCME (50 mg/kg) was identified as the most effective fraction.

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