Fluorescent Immunological Paper-based Assay for Exosome detection

Abstract This paper reports the development of fluorescent-linked immunosorbent paper-based assay for exosome detection. The paper-based device was fabricated with sandwich lamination for easy handling and was coated with exosome-specific antibody as a biosensing platform to detect exosome sample from the cell culture media. This assay employed fluorescent detection which is followed by tagging fluorophore-conjugated detecting antibody on exosome samples. The fluorescent readout was evaluated and quantified from image processing software. This assay can detect high concentration of exosome samples (~ 1010 exosome/mL). However, this assay has encountered various challenges. First, the exosome concentration prepared from cell culture media from cancer-derived ovarian and mesothelial cell lines may be insufficient to reach detectable range. Second, chemical contamination from exosome isolation kits may affect assay sensitivity. Therefore, assay optimization and minimizing chemical contamination are required which could enhance assay specificity and sensitivity.

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Cell culture media: NMR approaches to evaluating quality and nutrient consumption

Planta Medica ◽

10.1055/s-0036-1596271 ◽

2016 ◽

Vol 81 (S 01) ◽

pp. S1-S381

Author(s):

KB Killday ◽

AS Freund ◽

C Fischer ◽

KL Colson

Keyword(s):

Cell Culture ◽

Culture Media ◽

Cell Culture Media ◽

Nutrient Consumption

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Targeted metabolomics in the cell culture media reveals increased uptake of branched amino acids by breast cancer cells

Analytical Biochemistry ◽

10.1016/j.ab.2021.114192 ◽

2021 ◽

pp. 114192

Author(s):

Fang Kou ◽

Bangjie Zhu ◽

Wenbin Zhou ◽

Chunming Lv ◽

Yu Cheng ◽

...

Keyword(s):

Breast Cancer ◽

Amino Acids ◽

Cell Culture ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Culture Media ◽

Targeted Metabolomics ◽

Cell Culture Media ◽

Branched Amino Acids

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Selection and preliminary application of DNA aptamer targeting A549 excreta in cell culture media

Microchemical Journal ◽

10.1016/j.microc.2021.106811 ◽

2021 ◽

pp. 106811

Author(s):

Yuanbin Guo ◽

Ming Shi ◽

Xiujuan Liu ◽

Huagang Liang ◽

Liming Gao ◽

...

Keyword(s):

Cell Culture ◽

Culture Media ◽

Dna Aptamer ◽

Cell Culture Media ◽

Preliminary Application

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Benchmarking of commercially available CHO cell culture media for antibody production

Applied Microbiology and Biotechnology ◽

10.1007/s00253-015-6514-4 ◽

2015 ◽

Vol 99 (11) ◽

pp. 4645-4657 ◽

Cited By ~ 59

Author(s):

David Reinhart ◽

Lukas Damjanovic ◽

Christian Kaisermayer ◽

Renate Kunert

Keyword(s):

Cell Culture ◽

Antibody Production ◽

Culture Media ◽

Cho Cell ◽

Cell Culture Media ◽

Cho Cell Culture

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Intestinal and Hepatic Uptake of Dietary Peroxidized Lipids and Their Decomposition Products, and Their Subsequent Effects on Apolipoprotein A1 and Paraoxonase1

Antioxidants ◽

10.3390/antiox10081258 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1258

Author(s):

Xueting Jiang ◽

Pragney Deme ◽

Rajat Gupta ◽

Dmitry Litvinov ◽

Kathryn Burge ◽

...

Keyword(s):

Cell Culture ◽

Culture Media ◽

Degradation Products ◽

Apolipoprotein A1 ◽

Paraoxonase 1 ◽

Pon1 Activity ◽

Atherosclerotic Cardiovascular Disease ◽

Metabolic Fate ◽

Cell Culture Media ◽

Decomposition Products

Both pro- and antiatherosclerotic effects have been ascribed to dietary peroxidized lipids. Confusion on the role of peroxidized lipids in atherosclerotic cardiovascular disease is punctuated by a lack of understanding regarding the metabolic fate and potential physiological effects of dietary peroxidized lipids and their decomposition products. This study sought to determine the metabolic fate and physiological ramifications of 13-hydroperoxyoctadecadienoic acid (13-HPODE) and 13-HODE (13-hydroxyoctadecadienoic acid) supplementation in intestinal and hepatic cell lines, as well as any effects resulting from 13-HPODE or 13-HODE degradation products. In the presence of Caco-2 cells, 13-HPODE was rapidly reduced to 13-HODE. Upon entering the cell, 13-HODE appears to undergo decomposition, followed by esterification. Moreover, 13-HPODE undergoes autodecomposition to produce aldehydes such as 9-oxononanoic acid (9-ONA). Results indicate that 9-ONA was oxidized to azelaic acid (AzA) rapidly in cell culture media, but AzA was poorly absorbed by intestinal cells and remained detectable in cell culture media for up to 18 h. An increased apolipoprotein A1 (ApoA1) secretion was observed in Caco-2 cells in the presence of 13-HPODE, 9-ONA, and AzA, whereas such induction was not observed in HepG2 cells. However, 13-HPODE treatments suppressed paraoxonase 1 (PON1) activity, suggesting the induction of ApoA1 secretion by 13-HPODE may not represent functional high-density lipoprotein (HDL) capable of reducing oxidative stress. Alternatively, AzA induced both ApoA1 secretion and PON1 activity while suppressing ApoB secretion in differentiated Caco-2 cells but not in HepG2. These results suggest oxidation of 9-ONA to AzA might be an important phenomenon, resulting in the accumulation of potentially beneficial dietary peroxidized lipid-derived aldehydes.

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