scholarly journals CLONAL PROPAGATION OF LIATRIS PYCNOSTACHYA MICHX BY IN VITRO CULTURE OF AXILLARY BUDS

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1137c-1137
Author(s):  
Azza Tawfik ◽  
P. E. Read ◽  
S. S. Salac
Keyword(s):  
In Vitro Culture ◽  
Bacterial Contamination ◽  
Clonal Propagation ◽  
Shoot Length ◽  
Axillary Buds ◽  
Shoot Number ◽  
Bud Growth ◽  
Number Of Leaves ◽  
Axillary Bud Growth

A method is described for obtaining explants free of bacterial contamination and for clonal propagation by in vitro culture of liatris axillary buds. Axillary bud growth was stimulated by removal of the shoot tips of greenhouse grown stock plants. Prior to using this approach, extreme bacterial contamination occured when explants were taken from stock plants that had not been decapitated. However, these axillary buds (0.3-0.5 cm long) were successfully established free of bacterial contamination when excised, surface disinfested and cultured on Murashige & Skoog (MS) medium supplemented with various levels of benzyladenine (BA) or kinetin and gibberellic acid (GA3). The highest number of leaves and greatest shoot length were produced by buds cultured on a medium supplemented with 1.0 mg/l BA plus 1.5 mg/l GA3. Shoot number was increased on medium containing 1.0 or 2.0 mg/l BA plus 0.5 mg/l GA3. Kinetin significantly increased the leaf number of the buds but there was no effect of kinetin on the shoot length or number. Shoots formed roots in a medium supplemented with 3 mg/l indole-3-butyric acid (IBA) plus 9 mg/l GA3. The plantlets were transferred to vermiculite and acclimatized successfully under intermittent mist in a greenhouse.

scholarly journals Effect of different levels of NAA on in vitro growth and development of shoots of Dendrobium orchid

1970 ◽  
Vol 34 (3) ◽  
pp. 411-416 ◽  
Author(s):  
MS Parvin ◽  
ME Haque ◽  
F Akhter ◽  
M Moniruzzaman ◽  
ABM Khaldun
Keyword(s):  
Growth And Development ◽  
Shoot Proliferation ◽  
Shoot Length ◽  
In Vitro Growth ◽  
Shoot Number ◽  
Number Of Leaves ◽  
Shoot Weight ◽  
Different Levels ◽  
In Vitro Shoot Proliferation

The present study was conducted to investigate the effect of growth regulator NAA on in vitro shoot proliferation, rooting, and plantlet establishment. Among the different concentrations of NAA, the best increase in shoot weight (0.25 g) and shoot number (8.83) were observed from 0.1 mg/I NAA. The highest shoot length (2.60 cm), number of leaves (4.83), number of roots (5.15), and root length (2.67 cm) were obtained with 0.2 mg/I NAA at 60 DAT. Key Words: Dendrobium orchid, NAA, MS media. DOI: 10.3329/bjar.v34i3.3966 Bangladesh J. Agril. Res. 34(3) : 411-416, September 2009

scholarly journals In vitro clonal propagation and efficient acclimatization with use of hydrogel of intensively sweet medicinal plant Lippia dulcis Trev.

2020 ◽  
Vol 66 (4) ◽  
pp. 25-31
Author(s):  
Magdalena Tomaszewska-Sowa
Keyword(s):  
Leaf Area ◽  
Growth Regulators ◽  
Clonal Propagation ◽  
Shoot Length ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation ◽  
Number Of Leaves ◽  
In Vitro Clonal Propagation

Summary Introduction: The leaves of Lippia dulcis contain high amounts of hernandulcin. It is one thousand fold sweeter than sucrose, however, it hardly contains any calories. Objective: The aim of this research was to optimalisation of micropropagation and acclimatization of L dulcis Methods: The nodal explants were inoculated on phytohormone-free MS medium. After 6 weeks the explants were inoculated onto the MS medium with different plant growth regulators. Well-developed rooted plantlets were adapted to ex vitro conditions using hydrogel. Results: On the medium with BAP and NAA the highest number of shoots were produced. The higest average shoot length, number of the leaves and the leaf area were recorded on the medium with GA3. Adding IBA increased the number of roots. The addition of hydrogel enhanced the acclimatization efficiency. Conclusions: There was observed a positive, stimulating influence of growth regulators on mass propagation and increase in the number of leaves and the leaf area and influence of hydrogel on the development of plantlets during acclimatization.

Rapid clonal propagation of rhubarb by in vitro culture of shoot-tips

1979 ◽  
Vol 11 (3) ◽  
pp. 241-246 ◽  
Author(s):  
Joseph Roggemans ◽  
Marie-Christine Claes
Keyword(s):  
In Vitro Culture ◽  
Clonal Propagation ◽  
Shoot Tips

In vitro propagation of the bay laurel (Laurus nobilis.L) in Morocco

2014 ◽  
Vol 4 (3) ◽  
pp. 96-103
Author(s):  
Abdelali Chourfi ◽  
Tajelmolk Alaoui ◽  
Ghizlane Echchgadda
Keyword(s):  
Seed Germination ◽  
In Vitro Culture ◽  
In Vitro Propagation ◽  
Aerial Part ◽  
Basal Medium ◽  
Axillary Buds ◽  
Laurus Nobilis

Laurus nobilis L. is among the species which are most threatened by massive degradation in Morocco. The multiplication by seed or by cuttings gives very low percentages of recovery that is insufficient to meet the demand of growing market. In vitro culture proves to be a tremendous asset to solve this problem. Our work has focused on the study of seed germination of this species and its multiplication from microcuttings. Finally, we studied the ac-climatization ability of the plantlets resulting from this germination. The study of the germination, via the further measurement of the length of the aerial part and the roots and the number of axillary buds for nine weeks, showed that the MS basal medium was more efficient than media 1/2M.S and WPM. Among the eight tested hormones, IAA yielded the best growth of the plantlets. Hormonal combination of NAA and kinetin resulted into a per-centage of the greatest success in reaching 67 % micropropagation. The study also revealed that the MS basal medium in the presence of the IAA plants can acclimate most easily in two types of substrates with improved development in the peat alone.

scholarly journals In vitro callus initiation and regeneration of potato

1970 ◽  
Vol 34 (3) ◽  
pp. 449-456 ◽  
Author(s):  
AU Haque ◽  
MA Ehsanul Samad ◽  
TL Shapla
Keyword(s):  
Key Words ◽  
Root Length ◽  
Shoot Length ◽  
Leaf Explant ◽  
Callus Initiation ◽  
Number Of Leaves ◽  
Interaction Term ◽  
Shoot Tip Explants ◽  
Internode Explants

The first experiment involving different explants and concentrations of 2,4-D and kinetin showed highly significant differences for length and weight of callus formed except interaction of callus weight. Leaf explant appeared to be best of all for callus length and weight when 1.0 mg/L 2,4-D + 0.25 mg/L kinetin concentration was used. Similarly, different explants versus different concentrations of BAP/GA3/IAA showed significant differences for shoot length and leaf number per plantlet and also for root length. However, interaction term confirmed node and node/internode explants produced better results in shoot length and number of leaves per plantlet when concentrations 1 .0 mg/L BAP + 0.1 mg/L GA3 and 1.0 mg/L BAP + 0.2 mg/L GA3, 1.0 mg/L BAP + 0.4 mg/L GA3, respectively, were used. Similarly, internode explants produced better results for root length after 21 days plantlet-1 when concentration of 1.0 mg/L IAA + 0.25 mg/L GA3 was used. Shoot tip explants also produced better results in root length after 28 days plantlet-1 when concentrations 1.0 mg/L IAA + 0.25 mg/L GA3 were used. Key Words: In vitro, callus initiation, regeneration, potato. DOI: 10.3329/bjar.v34i3.3971 Bangladesh J. Agril. Res. 34(3) : 449-456, September 2009

scholarly journals Biosynthesis and characterization of silver nanoparticles using Ochradenus arabicus and their physiological effect on Maerua oblongifolia raised in vitro

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hassan O. Shaikhaldein ◽  
Fahad Al-Qurainy ◽  
Mohammad Nadeem ◽  
Salim Khan ◽  
Mohamed Tarroum ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Chlorophyll Content ◽  
Total Protein ◽  
Dry Weight ◽  
Shoot Length ◽  
Antioxidant Enzyme Activities ◽  
Fresh Weight ◽  
Wide Range ◽  
Shoot Number

Abstract Silver nanoparticles (AgNPs) are presently the most commonly generated engineered nanomaterials and are found in a wide range of agro-commercial products. The present study was designed to synthesize AgNPs biologically using Ochradenus arabicus leaves and investigate their effect on the morphophysiological properties of Maerua oblongifolia raised in vitro. Physicochemical methods (ultraviolet–visible spectroscopy, Fourier transform infrared spectroscopy, and transmission electron microscopy were performed for characterization and for obtaining microphotographs of the AgNPs. Shoots of M. oblongifolia (2–3 cm) grown in Murashige and Skoog medium supplemented with different concentrations of AgNPs (0, 10, 20, 30, 40, or 50 mg L−1) were used. Following 6 weeks of in vitro shoot regeneration, the shoot number, shoot length, leaf number, fresh weight, dry weight, chlorophyll content, total protein, proline level, and antioxidant enzyme activities of the plants were quantified. We found that 20 mg L−1 AgNPs increased the shoot number, shoot length, fresh weight, dry weight, and chlorophyll content of the plants. The maximum total protein was recorded in plants that were administered the lowest dose of AgNPs (10 mg L−1), while high concentrations of AgNPs (40 and 50 mg L−1) increased the levels of proline and the enzymes superoxide dismutase and catalase. Our results indicate that green-synthesized AgNPs may be of agricultural and medicinal interest owing to their effects on plants in vitro.

scholarly journals Transcriptomic and physiological analyses of rice seedlings under different nitrogen supplies provide insight into the regulation involved in axillary bud outgrowth

2020 ◽  
Author(s):  
Rongna Wang ◽  
Junjie Qian ◽  
Zhongming Fang ◽  
Jihua Tang
Keyword(s):  
Growth Rate ◽  
Morphological Changes ◽  
Expression Profiles ◽  
Axillary Buds ◽  
Axillary Bud ◽  
Rice Seedlings ◽  
Transcriptomic Data ◽  
Bud Growth ◽  
Axillary Bud Growth ◽  
Axillary Bud Outgrowth

Abstract Background: N is an important macronutrient required for plant development and significantly influences axillary bud outgrowth, which affects tillering and grain yields of rice. However, how different N concentrations affect axillary bud growth at the molecular and transcriptional levels remains unclear. Results: In this study, morphological changes in the axillary bud growth of rice seedlings under different N concentrations ranging from low to high levels were systematically observed. To investigate the expression of N-induced genes involved in axillary bud growth, we used RNA-seq technology to generate mRNA transcriptomic data from two tissue types, basal parts and axillary buds, of plants grown under six different N concentrations. In total, 10,221 and 12,180 DEGs induced by LN or HN supplies were identified in the basal parts and axillary buds, respectively, via comparisons to expression levels under NN level. Analysis of the coexpression modules from the DEGs of the basal parts and axillary buds revealed an abundance of related biological processes underlying the axillary bud growth of plants under N treatments. Among these processes, the activity of cell division and expansion was positively correlated with the growth rate of axillary buds of plants grown under different N supplies. Additionally, TFs and phytohormones were shown to play crucial roles in determining the axillary bud growth of plants grown under different N concentrations. Further validation of OsGS1;2 and OsGS2 , the rice mutants of which presented altered tiller numbers, validated our transcriptomic data. Conclusion: These results indicate that different N concentrations affect the axillary bud growth rate, and our study revealed comprehensive expression profiles of genes that respond to different N concentrations, providing an important resource for future studies attempting to determine how axillary bud growth is controlled by different N supplies.

scholarly journals In vitro Multiple Shoot Regeneration from Petunia hybrida

2019 ◽  
Vol 7 (10) ◽  
pp. 1554
Author(s):  
Rebaz Rasul Habas ◽  
Musa Turker ◽  
Fethi Ahmet Ozdemir
Keyword(s):  
Petunia Hybrida ◽  
Survival Rates ◽  
Basal Medium ◽  
Multiple Shoot ◽  
Shoot Length ◽  
Peat Moss ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Shoot Number

An efficient plant regeneration protocol was developed from in vitro germinated seeds of Petunia hybrida an ornamentally important plant in the family Solanaceae. Shoot tip and node explants of Petunia hybrida were cultured on MS basal medium supplemented with different concentrations and combinations of Benzyl amino purine (BAP), 1-Naphthaleneacetic acid (NAA), Indole-3-butyric acid (IBA) and Gibberellic acid (GA3). The highest shoot length was obtained from MS medium supplemented with 1 mg/l BAP + 1 mg/l NAA. The highest shoot number (3 shoots/explant) were obtained from MS medium supplemented with 0.6 mg/l BAP + 0.5 mg/l IBA. The isolated shoots were transferred to MS basal medium supplemented with different concentrations of GA3 ranging from 0.05, 0.2, 0.5 and 1 mg/l for shoot elongation. The highest shoot length (5.75 cm) was recorded from the MS medium supplemented with 0.2 mg/l GA3 +0.2 mg/l BAP. Rooting of regenerated shoots were achieved on MS medium supplemented with 0.1-1 mg/1 IBA and NAA. The regenerated shoots with well developed roots were successfully acclimatized and established in pots containing sterilized peat moss and grown under laboratory conditions with 70% survival rates.

scholarly journals 606 In Vitro Propagation of Some Strawberry Cultivars in Response to Kind and Concentration of Some Cytokinin Compounds

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 501D-501
Author(s):  
M.H. Aboul-Nasr ◽  
S.Z. Elagamy ◽  
A.M. Kassim
Keyword(s):  
In Vitro Propagation ◽  
Callus Formation ◽  
Shoot Length ◽  
Control Treatment ◽  
Fresh Weight ◽  
Shoot Height ◽  
Murashige And Skoog Medium ◽  
Number Of Leaves ◽  
Strawberry Cultivars

Three strawberry cultivars were used in this study. Runners from the three cultivars were grown on Murashige and Skoog medium supplemented with benzylaminpurine or kinetin at four concentrations (2, 1, 0.5, and 0.25 mg·L-1 in addition to the control treatment). The use of cytokinin, regardless of type and concentration, promoted the explant proliferation, shoot height, number of leaves, and plantlet fresh weight. Callus formation was enhanced by BA application, especially with `Pajaro'. However, `Chandler' did not form at all using cytokinin. Produced plantlets had lower nutrient constituents (N, P, K, Ca, and Na) when grown on media containing cytokinin compared to the control. Based on these results, cytokinin is recommended to get more shoots per explant. Furthermore, using the lowest concentration (0.25 mg·L-1) will produce height proliferation, greater shoot length, and more leaves.

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