Epibrassinolide Does Not Enhance Heat Shock Tolerance and Antioxidant Activity in Moth Bean
Moth bean (Vigna aconitifolia Jacqu. Marecbal cv. Jaadia) seeds were germinated in 0, 0.1, 1, or 2 μm EBL. After 72 hours, seedlings were exposed to 22 or 48C for 90 minutes. At 48C, EBL increased total electrolyte, K+, and sugar leakage from the seedlings relative to the control. Following exposure to 48C, EBGtreated seedlings bad higher malondialdebyde concentrations than controls, indicating that EBL enhanced high-temperature-induced lipid peroxidation. At 48C, EBL increased ascorbic acid oxidase activity but decreased superoxide dismutase activity relative to the control. Taken collectively, these data do not support a hypothesis that brassinosteroids confer beat shock tolerance to moth bean. Chemical name used: 24-epibrassinolide (EBL).