scholarly journals THERMOTOLERANCE AND ANTIOXIDANT ACTIVITY OF MOTH BEAN SEEDLINGS AS INFLUENCED BY 24-EPIBRASSINOLIDE

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1082d-1082
Author(s):  
Abha Upadhyaya ◽  
Tim D. Davis ◽  
Narendra Sankhla
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
High Temperature ◽  
Cellular Damage ◽  
Antioxidant Systems ◽  
Acid Oxidase ◽  
Vigna Aconitifolia ◽  
Moth Bean

Seeds of moth bean (Vigna aconitifolia Jacqu. Marechal cv. Jaadia) were germinated in the presence of 0, 0.1, 1, or 2 μm 24-epibrassinolide (EBL). After 72 h, cotyledons were excised and the seedlings exposed to 22 or 48 °C for 90 min. At 48 °C EBL increased total electrolyte, K+, and sugar leakage relative to the untreated control. Following exposure to 48 °C, EBL-treated seedlings had higher malondialdehyde concentrations than controls indicating that EBL enhanced high temperature-induced lipid peroxidation. At 48 °C, EBL increased ascorbic acid oxidase activity and decreased superoxide dismutase activity relative to the control. Taken together, these data do not support the hypothesis that brassinosteroids confer thermotolerance to plants. On the contrary, EBL increased high temperature-induced damage and reduced the activity of some antioxidant systems that may protect against stress-induced cellular damage.

scholarly journals Epibrassinolide Does Not Enhance Heat Shock Tolerance and Antioxidant Activity in Moth Bean

HortScience ◽  
1991 ◽  
Vol 26 (8) ◽  
pp. 1065-1067 ◽  
Author(s):  
Abha Upadhyaya ◽  
Tim D. Davis ◽  
Narendra Sankhla
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
High Temperature ◽  
Heat Shock ◽  
Oxidase Activity ◽  
Acid Oxidase ◽  
Vigna Aconitifolia ◽  
Moth Bean

Moth bean (Vigna aconitifolia Jacqu. Marecbal cv. Jaadia) seeds were germinated in 0, 0.1, 1, or 2 μm EBL. After 72 hours, seedlings were exposed to 22 or 48C for 90 minutes. At 48C, EBL increased total electrolyte, K+, and sugar leakage from the seedlings relative to the control. Following exposure to 48C, EBGtreated seedlings bad higher malondialdebyde concentrations than controls, indicating that EBL enhanced high-temperature-induced lipid peroxidation. At 48C, EBL increased ascorbic acid oxidase activity but decreased superoxide dismutase activity relative to the control. Taken collectively, these data do not support a hypothesis that brassinosteroids confer beat shock tolerance to moth bean. Chemical name used: 24-epibrassinolide (EBL).

scholarly journals In-vitro and In-vivo Antioxidant Activity of Ethanol Leaf Extract of Justicia carnea

2020 ◽  
pp. 48-60
Author(s):  
Udedi Stanley Chidi ◽  
Ani Onuabuchi Nnenna ◽  
Asogwa Kingsley Kelechi ◽  
Maduji Fitzcharles Chijindu ◽  
Okafor Clinton Nebolisa
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Leaf Extract ◽  
Dpph Radical ◽  
Β Carotene ◽  
In Vitro Antioxidant Activity

This study investigated the in-vitro antioxidant activity of ethanol leaf extract of Justicia carnea and its effect on antioxidant status of alloxan-induced diabetic albino rats. The in-vitro antioxidant activity was assayed by determining the total phenol, flavonoids, ascorbic acid, β-carotene and lycopene contents and by using 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical, reducing antioxidant power and inhibition of lipid peroxidation antioxidant systems. Oxidative stress was produced in rats by single intraperitoneal injection of 150 mg/kg alloxan and serum concentration of malonaldehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were determined. Five experimental groups of rats (n=6) were used for the study. Two groups of diabetic rats received oral daily doses of 100 and 200 mg/kg Justicia carnea leaf extract respectively while gilbenclamide (5 mg/ml); a standard diabetic drug was also given to a specific group for 14 days. From the result, the leaf extract contained a higher concentration of flavonoids followed byphenols, ascorbic acid, lycopene and β-carotene. The extract displayed more potent reducing power ability with EC50 of 40 µg/ml compared to BHA (EC50 of 400µg/ml). The percentage DPPH radical scavenging activity of the extract was also higher with EC50 of 200µg/ml and increased with increase in concentration while BHA had EC50of 320µg/ml. The inhibition of lipid peroxidation also increased with increase in concentration with EC50 of 58µg/ml and comparable with BHA (EC50=60µg/ml). The effect of the plant extract on antioxidant enzyme activities was concentration-dependent. Administration of 100mg/kg of the plant extract resulted in a significant decrease (p<0.05) in serum MDA concentration, while 200 mg/kg of the extract caused a significant (p˂0.05) increase in superoxide dismutase (SOD) and catalase activities with a non-significant increase (p>0.05) in the serum level of MDA when compared with the diabetic untreated group. These findings suggest that ethanol leaf extract of Justicia carnea have antioxidant properties and could handle diabetes-induced oxidative stress.

scholarly journals THE STATE OF THE ANTIOXIDANT SYSTEM OF THE INTERNAL ORGANS IN RATS DURING BURN DISEASE

2014 ◽  
Vol 13 (3) ◽  
pp. 51-55
Author(s):  
L. G. Netyukhailo ◽  
T. A. Sukhomlin ◽  
Ya. A. Basarab ◽  
V. V. Bondarenko ◽  
S. V. Kharchenko
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Salivary Glands ◽  
Antioxidant System ◽  
The State ◽  
Antioxidant Systems ◽  
Free Radical Processes ◽  
Burn Disease ◽  
Radical Processes

The objective of research was to study the state of prooxidant and antioxidant systems in the tissues of the lungs, kidneys, pancreas and salivary glands at burn disease. The intensity of the free radical processes was evaluated on the basis of the content of malondialdehyde (MDA) and antioxidant system – based on the indexes of its enzymatic chain: superoxide dismutase and catalase in homogenates of the studied organs. It has been found that changes in experimental burn disease depend on the studied organs and the stage of burn disease. The activation of free radical processes observed in all investigated organs (lungs, kidneys, pancreas and salivary glands). Reactive oxygen species induce lipid peroxidation, which is a universal marker of tissue damage. MDA appears in the body during degradation of polyunsaturated fatty acids and it’s a marker of lipid peroxidation and oxidative stress. It was found the increasing of MDA in all organs, especially in the lungs and kidneys at stage of burn shock. Under these conditions it was observed the decrease of superoxide dismutase and catalase in all investigated organs. At burn disease there is development of disbalance between the action of prooxidant and antioxidant systems due to the activation of free radical processes.

scholarly journals Comparative Study of the Antioxidant Activities of Eleven Salvia Species

2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Gábor Janicsák ◽  
István Zupkó ◽  
Imre Máthé ◽  
Judit Hohmann
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Biological System ◽  
Antioxidant Activities ◽  
Close Correlation ◽  
Reference Compound ◽  
Total Polyphenol ◽  
Methanolic Extracts

The lipid peroxidation-inhibiting activities of aqueous methanolic extracts of eleven Salvia species (Fam. Lamiaceae) were evaluated in an enzyme-independent biological system. The total polyphenol contents and the amounts of the most abundant phenoloids of the genus, caffeic and rosmarinic acids, were also determined. The EC50 values of the extracts displayed substantial differences. All of the investigated species except S. jurisicii (EC50 191.2 μg/mL) exhibited higher activities than that of ascorbic acid (EC50 123.8 μg/mL), the reference compound. Among the studied species, S. scabiosifolia (EC50 5.4 μg/mL) demonstrated the highest effect, followed in sequence by S. dumetorum, S. transsylvanica, S. officinalis l albiflora, S. nemorosa l albiflora and S. recognita (EC50 6.5 – 10.2 μg/mL). The close correlation was confirmed between the antioxidant activities and the total phenol contents of the extracts. For caffeic and rosmarinic acids, the correlation was much weaker, indicating the important role of other polyphenols in the antioxidant activity.

scholarly journals Retinoic acid modulation of glutathione and cysteine metabolism in chondrocytes

1996 ◽  
Vol 314 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Cristina C. TEIXEIRA ◽  
Irving M. SHAPIRO ◽  
Masashi HATORI ◽  
Ramesh RAJPUROHIT ◽  
Cameron KOCH
Keyword(s):  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Retinoic Acid ◽  
Critical Role ◽  
Phosphate Concentration ◽  
Antioxidant Systems ◽  
Chondrocyte Maturation ◽  
Medium Components ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid

The major objective of this investigation was to determine the thiol status of chondrocytes and to relate changes in the level of glutathione and cysteine to maturation of the cells as they undergo terminal differentiation. Chondrocytes were isolated from the cephalic portion of chick embryo sterna and treated with all-trans retinoic acid for one week. We found that the addition of 100 nM retinoic acid to the cultures decreased the intracellular levels of glutathione and cysteine from 6.1 to 1.6 and 0.07 to 0.01 nmol/μg DNA respectively; retinoic acid also caused a decrease in the extracellular concentration of cysteine. The decrease in chondrocyte thiols was dose and time dependent. To characterize other antioxidant systems of the sternal cell culture, the activities of catalase, glutathione reductase and superoxide dismutase were determined. Activities of all of those enzymes were high in the retinoic acid-treated cells; the conditioned medium also contained these enzymes and the cytosolic isoenzyme of superoxide dismutase. We probed the specificity of the thiol response by using immature caudal chondrocytes. Unlike the cephalic cells, retinoic acid did not change intracellular glutathione and extracellular cysteine levels, although the retinoid caused a reduction in the intracellular cysteine concentration. Finally, we explored the effect of medium components on chondrocyte thiol status. We noted that while ascorbate alone did not change cell thiol levels, it did cause a 4-fold decrease in the extracellular cysteine concentration. When retinoic acid and ascorbic acid were both present in the medium, there was a marked decrease in the level of glutathione. In contrast, the phosphate concentration of the culture medium served as a powerful modulator of both glutathione and cysteine. Results of the study clearly showed that there is a profound decrease in intracellular levels of both cysteine and glutathione and that thiol levels are responsive to ascorbic acid and the medium phosphate concentration. These findings point to a critical role for thiols in modulating events linked to chondrocyte maturation and cartilage matrix synthesis and mineralization.

A Study on the Antioxidant Activity and Tissues Selective Inhibition of Lipid Peroxidation by Saponins from the Roots of Platycodon grandiflorum

2009 ◽  
Vol 37 (05) ◽  
pp. 967-975 ◽  
Author(s):  
Xian-Jun Fu ◽  
Hong-Bing Liu ◽  
Peng Wang ◽  
Hua-Shi Guan
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Selective Inhibition ◽  
Antioxidant Systems ◽  
Platycodon Grandiflorum ◽  
Platycodi Radix ◽  
Antioxidant Effects ◽  
Different Tissues ◽  
Selective Effects

Platycodi Radix is the root of Platycodon grandiflorum (Jacq.) A. DC and has been used as a traditional medicine in China. According to the theory of traditional Chinese medicine, Platycodi Radix (PR) possesses the character of lung meridian tropism (Guijing) and has selective effects on the lung and respiratory system. The aim of this study was to confirm the antioxidant effects of saponins from Platycodi Radix (PRS), with emphasis on its selective inhibition of lipid peroxidation in different tissues. The hydroxyl radical scavenging activity was determined by a H2O2/Fe2+ system; a modified thiobarbituric acid reactive species assay was used to measure the lipid peroxide in rats' tissues. These antioxidant activities were compared to ascorbic acid (Vc). The results showed that PRS had antioxidant activities in various antioxidant systems. And the inhibition capability of lipid peroxidation of PRS and Vc were excellent, but differed greatly in different tissues. These results suggested that PRS had antioxidant effects and selective inhibition of lipid peroxidation. It indicated that the mechanism of Platycodi Radix to treat some diseases might be related to its antioxidant activity, especially its tissue selective effects. However, further study is needed.

Antioxidant Activity of Enzymatic Hydrolysates of High-Temperature Soybean Meal Pretreated with High Pressures

2015 ◽  
Vol 1092-1093 ◽  
pp. 1519-1524 ◽  
Author(s):  
Jing Xu ◽  
Yu Ting Wang ◽  
Xiao Yu Wang ◽  
Xin Ru Li ◽  
Qian Xin Dang
Keyword(s):  
Antioxidant Activity ◽  
High Pressure ◽  
Superoxide Dismutase ◽  
High Temperature ◽  
Glutathione Peroxidase ◽  
Molecular Mass ◽  
Soybean Meal ◽  
High Pressures ◽  
Relative Molecular Mass ◽  
Mda Content

Effect of high-temperature soybean meal hydrolysates was to be studied in this paper. Hhigh-temperature soybean meal was treated by high pressure. And Alcalase 2.4 L was used to hydrolyze high-temperature soybean meal, three kinds of solutions with relative molecular mass of > 10 000 Da, 5000 Da – 10 000 Da and < 5 000 Da were obtained by ultrafiltration of hydrolysates, and they were administrated mice by gastric perfusion, respectively. Levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) in liver were separately tested with reagent kits. Results showed that SOD and GSH-PX activities were significantly improved and MDA content was reduced in liver of mice by hydrolysates, which indicated that high-temperature soybean meal hydrolysates can improve antioxidant indexes of mice and enhance antioxidation capacity of body.

scholarly journals Aluminum Affects the Antioxidant Activity of Tobacco Cells and the Expression of Salicylate-Induced Protein Kinase (SIPK)

2011 ◽  
Vol 39 (2) ◽  
pp. 77 ◽  
Author(s):  
Atefeh PAYEZ ◽  
Faezeh GHANATI ◽  
Seyed Mahdi SEYEDI
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Protein Kinase ◽  
Radical Scavenging ◽  
Signal Transduction Pathway ◽  
Al Toxicity ◽  
Antioxidant Systems ◽  
Short Treatment ◽  
Mapk Activation ◽  
Tobacco Cells

It has been shown that Aluminum (Al) toxicity results in over-production of reactive oxygen species (ROS). Despite the fact that ROS can induce MAPK activation, no direct genetic evidence has linked ROS-induced MAPK activation with Al toxicity. The effect of Al on the activation of antioxidant systems in connection with the expression of salicylate-induced protein kinase (SIPK), was examined in tobacco cells. Suspension-cultured tobacco cells (Nicotiana tabacum L. cv. ‘Barley 21’) were treated with or without 80 μM Al. Certain parameters related to antioxidant activity were measured. A MAPKK inhibitor (PD98059) was also applied together with or without Al treatments and semi quantitative RT-PCR was applied to show the expression level of the SIPK gene. Treatment with Al rapidly increased the radical scavenging capacity of cells, catalase and superoxide dismutase activities, and expression of the SIPK gene, as compared to the control cells. In the presence of Al + PD98059, a decrease in catalase and superoxide dismutase activities was observed, compared to those cells which were treated only with Al. These results suggested that a short treatment with Al induced the activity of certain antioxidant enzymes in tobacco cells and that this response was mediated by a MAPK signal transduction pathway.

scholarly journals Prooxidant-Antioxidant Balance in the Heart of Rats with Different Behavioral Activities under the Conditions of Light Exposition

2021 ◽  
Vol 6 (3) ◽  
pp. 360-365
Author(s):  
L. D. Chebotar ◽  
◽  
E. N. Laricheva ◽  
M. Sh. Gilmutdinova
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Open Field ◽  
Emotional Stress ◽  
Light Exposure ◽  
Superoxide Dismutase Activity ◽  
Main Role ◽  
Experimental Animals ◽  
Behavioural Activity

The purpose of the article. The article shows that the effect of round-the-clock lighting causes changes in the processes of lipid peroxidation and antioxidant activity in rats, and depends on their behavioural activity. The effects of lighting on the processes of oxidative metabolism of varying degrees in the heart of resistant and unstable to emotional stress rats. Materials and methods. Investigations were carried out on 32 mature Wistar rats, divided into four groups: group 1 – animals resistant to emotional stress (intact); group 2 – intact animals unstable to emotional stress; group 3 – experimental animals resistant to emotional stress (30-day light exposure (1000 lux)); group 4 – experimental animals, unstable to emotional stress (30-day light exposure (1000 lux)). When assessing the effect of light on the state of the organism, the most important integral indicator is the behavior of animals. Therefore, during the experiment we used the observation of behavioral reactions in the test “open field”. Based on the characteristics of the behavior of animals in the “open field” rats were divided into groups resistant and unstable to emotional stress. To assess lipid peroxidation in the heart homogenate, the concentration of TBA-active products, the concentration of diene, oxidiene and triene conjugates were determined. Antioxidant processes were assessed by the increase in the concentration of TBA- active products during 1.5-hour incubation in an iron-ascorbate buffer solution, as well as by the activity of superoxide dismutase and catalase. Results and discussion. Prooxidant activity was characterized by an increase in the concentration of TBA-reactive substances in animals resistant to emotional stress. The concentration of TBA-reactive substances after 1.5-hour incubation increased in both experimental groups. Changes in the antioxidant status were illustrated by an increase in superoxide dismutase activity in the group of stress-unstable rats, whereas catalase activity increased in both experimental groups. In addition, in the group of animals resistant to emotional stress, a significant decrease in the resources of α-tocopherol and β-carotene was revealed. Conclusion. The long-term light exposure promotes the formation of end products of peroxidation in the heart of rats resistant to emotional stress and causes a decrease in antioxidant potential, regardless of behavioural activity. Antioxidant activity in the heart of emotionally stress-resistant rats is realized through both the enzyme and non-enzyme links of the antioxidant defence, while the main role in the heart of emotionally stress-resistant rats is played by superoxide dismutase activity

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