scholarly journals Seasonal Expression of a 700–800-Base Pair Transcript in Bark Tissues of Peach (Prunus persica)

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 514B-514
Author(s):  
Michael Wisniewski ◽  
Tim Artlip ◽  
Carole Bassett ◽  
Ann Callahan
Keyword(s):  
Fruit Development ◽  
Seasonal Changes ◽  
Woody Plants ◽  
Cold Hardiness ◽  
Prunus Persica ◽  
Storage Proteins ◽  
Proteinase Inhibitors ◽  
Peach Fruit ◽  
Genes Encoding

Cold acclimation in temperate, woody plants involves distinct changes in gene activity and protein expression. We have been identifying proteins and genes that are associated with seasonal changes in cold hardiness. Seasonal changes in a 60-kDa dehydrin and its corresponding transcript have been identified, as well as seasonal changes in 16- and 19-kDa storage proteins. Further screening of a cDNA library, constructed from cold-acclimated bark tissues collected in December, identified a 700–800-bp clone that was seasonally expressed in Northern blots. The transcript began to accumulate in October, reached a peak in November–December, and then began to decline. By April, the transcript was no longer present in bark tissues. The transcript size indicates that this gene my be related to either the 16- or 19-kDa storage proteins previously identified; however, an amino acid sequence of the protein for comparison has not yet been obtained. Interestingly, the transcript is also expressed during the early stages of peach fruit development. A similar pattern between seasonal expression and fruit development has been observed for a peach dehydrin transcript. Analysis of a partial sequence of the clone has indicated a similarity to genes encoding proteinase inhibitors and thionins (a class of biocidal proteins). More definitive characterization of the gene and identification of its corresponding protein are in progress.

scholarly journals Isolation and Initial Characterization of cDNAs for mRNAs Regulated during Peach Fruit Development

1993 ◽  
Vol 118 (4) ◽  
pp. 531-537 ◽  
Author(s):  
Ann M. Callahan ◽  
Peter H. Morgens ◽  
Reuben A. Cohen
Keyword(s):  
Fruit Development ◽  
Prunus Persica ◽  
Cdna Libraries ◽  
Cdna Clones ◽  
Ripe Fruit ◽  
Peach Fruit ◽  
Initial Characterization ◽  
Initial Source ◽  
Softening Stage

Peach [Prunus persica (L.) Batsch] cDNA libraries have been constructed from RNA isolated from immature (30 days after bloom) and ripe fruit. cDNA clones of interest have been identified by differential hybridization among the cDNAs of various peach cultivars or from several stages in fruit development. In addition, several clones were isolated by low stringency hybridizations with oligonucleotides derived from a tomato polygalacturonase cDNA sequence and a cucumber peroxidase amino acid sequence. The pattern of accumulation of the corresponding mRNAs during fruit development was examined by RNA gel-blot analyses in the commercial cultivar Suncrest. Three cDNA clones, pch201, pch307, and pch313, were related to mRNAs that accumulate during the softening stage of fruit development. cDNA clones pchl03, pch205, and pch306 were related to an mRNAs that increase in abundance throughout development, with maximum levels in ripe fruit. cDNA clones pch104 and pch202 were related to mRNAs that exhibit maximum abundance in midfruit development, and clone pch108 was related to mRNA that decreases as the fruit matures. Southern analyses indicated that seven of the cDNAs are represented by only a few genes, while pch104 detects a repetitive family, and pch307 detects a small family of genes. These clones will provide the initial source of genes to manipulate and affect fruit development.

scholarly journals Characterization of a Type II Chlorophyll a/b-binding Protein Gene (Lhcb2*Pp1) in Peach: I. Isolation, Identification, and Abundance in Developing Leaves of Mature `Loring' Trees in the Absence of Flowering

1998 ◽  
Vol 123 (4) ◽  
pp. 486-492 ◽  
Author(s):  
Carole Bassett ◽  
Ann Callahan ◽  
Linda Dunn
Keyword(s):  
Prunus Persica ◽  
Protein Gene ◽  
Binding Protein ◽  
Amino Acid Level ◽  
Growing Season ◽  
Type I ◽  
Type Ii ◽  
Genes Encoding ◽  
Binding Protein Gene

A gene (Lhcb2*Pp1) encoding a type II chlorophyll a/b-binding protein (LHCB) was isolated from peach [Prunus persica (L.) Batsch]. The gene was sequenced and compared to a variety of other genes encoding LHCB polypeptides associated with photosystem II. Similarity at the nucleotide and amino acid level was highest between Lhcb2*Pp1 and other type II genes and was lowest with type I and III genes from other species. Expression of Lhcb2*Pp1 was followed by determining abundance of transcripts in developing leaves of field grown trees in the absence of flowering. Expression was monitored at three times during the first phase of the growing season and was shown to be highest in leaves which were at or near full expansion at each sampling time. These results are consistent with the function of this gene and indicate that it can serve as a marker of photosynthetic maturity under field conditions.

scholarly journals Unravelling the Molecular Regulation Mechanisms of Slow Ripening Trait in Prunus persica

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2380
Author(s):  
Gerardo Núñez-Lillo ◽  
Lissette Ulloa-Zepeda ◽  
Catalina Pavez ◽  
Anibal Riveros ◽  
Francisca Blanco-Herrera ◽  
...  
Keyword(s):  
Fruit Development ◽  
Prunus Persica ◽  
Single Gene ◽  
The Other ◽  
Molecular Regulation ◽  
Biosynthetic Pathways ◽  
Terpene Biosynthesis ◽  
Peach Fruit ◽  
Complex Process ◽  
Regulation Mechanisms

Fruit development is a complex process that involves the interplay of cell division, expansion, and differentiation. As a model to study fruit development, nectarines incapable of ripening were described as slow ripening. Slow ripening fruits remained firm and exhibited no rise in CO2 or ethylene production rates for one month or more at 20 °C. Different studies suggest that this trait is controlled by a single gene (NAC072). Transcriptome analysis between normal and slow ripening fruits showed a total of 157, 269, 976, and 5.224 differentially expressed genes in each fruit developmental stage analyzed (T1, T2, T3, and T7, respectively), and no expression of NAC072 was found in the slow ripening individuals. Using this transcriptomic information, we identified a correlation of NAC072 with auxin-related genes and two genes associated with terpene biosynthesis. On the other hand, significant differences were observed in hormonal biosynthetic pathways during fruit development between the normal and slow ripening individuals (gibberellin, ethylene, jasmonic acid and abscisic acid). These results suggest that the absence of NAC072 by the direct or indirect expression or control of auxins or terpene-related genes prevents normal peach fruit development.

scholarly journals Characterization of two putative ethylene receptor genes expressed during peach fruit development and abscission

2002 ◽  
Vol 53 (379) ◽  
pp. 2333-2339 ◽  
Author(s):  
A. Rasori ◽  
B. Ruperti ◽  
C. Bonghi ◽  
P. Tonutti ◽  
A. Ramina
Keyword(s):  
Fruit Development ◽  
Ethylene Receptor ◽  
Peach Fruit

scholarly journals Seasonal Patterns of Dehydrins in Bark Tissue of Eight Species of Woody Plants

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 851A-851 ◽  
Author(s):  
Michael Wisniewski ◽  
Rajeev Arora ◽  
Tim Artlip
Keyword(s):  
Woody Plants ◽  
Cold Hardiness ◽  
Prunus Persica ◽  
Protein Extraction ◽  
Seasonal Patterns ◽  
Molecular Weights ◽  
Sassafras Albidum ◽  
Herbaceous Crops ◽  
Bark Tissue ◽  
Salix Babylonica

Studies with herbaceous crops have indicated a similarity in the types of proteins that accumulate in response to environmental stresses and ABA. Many of these proteins belong to a group called dehydrins. We have identified a 60 kDa dehydrin-related protein (PCA 60) in peach associated with cold hardiness. Our objective was to determine if seasonal induction of dehydrins are a common feature in a wide array of woody plants Bark tissues from eight species of woody plants were collected monthly for 1.5 years. The species included: Prunus persica `Loring'; Malus domestica `Golden Delicious'; Rubus sp. `Chester'; Populus sp.; Salix babylonica; Cornus florida; Sassafras albidum, and Robinia Pseudo-acacia. Protein extraction, SDSPAGE, and immunoblotting were performed as previously reported. Immunoblots were probed with a polyclonal antibody recognizing a conserved region of dehydrin proteins (provided by Timothy Close). Although some proteins, immunologically related to dehydrins, appeared lo be constitutive, distinct seasonal patterns associated with winter acclimation were observed in all species. The molecular weights of these proteins varied, although there were similarities in related species (willow and poplar). Although this study represents a precursory examination of dehydrins, the results indicate that these proteins are common to woody plants and that further research to characterize their function is warranted.

Isolation and characterization of the genes encoding antimicrobial neutrophil cationic peptides, defensins.

Ensho ◽  
1995 ◽  
Vol 15 (1) ◽  
pp. 33-41
Author(s):  
Isao Nagaoka ◽  
Noriko Ishihara ◽  
Akimasa Someya ◽  
Kazuhisa Iwabuchi ◽  
Shin Yomogida ◽  
...  
Keyword(s):  
Cationic Peptides ◽  
Isolation And Characterization ◽  
Genes Encoding

scholarly journals Protein complex formation in methionine chain-elongation and leucine biosynthesis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Li-Qun Chen ◽  
Shweta Chhajed ◽  
Tong Zhang ◽  
Joseph M. Collins ◽  
Qiuying Pang ◽  
...  
Keyword(s):  
Protein Complexes ◽  
Complete Characterization ◽  
Bimolecular Fluorescence Complementation ◽  
Chain Elongation ◽  
Substrate Channeling ◽  
Leucine Biosynthesis ◽  
Protein Complex Formation ◽  
Genes Encoding ◽  
Isopropylmalate Dehydrogenase

AbstractDuring the past two decades, glucosinolate (GLS) metabolic pathways have been under extensive studies because of the importance of the specialized metabolites in plant defense against herbivores and pathogens. The studies have led to a nearly complete characterization of biosynthetic genes in the reference plant Arabidopsis thaliana. Before methionine incorporation into the core structure of aliphatic GLS, it undergoes chain-elongation through an iterative three-step process recruited from leucine biosynthesis. Although enzymes catalyzing each step of the reaction have been characterized, the regulatory mode is largely unknown. In this study, using three independent approaches, yeast two-hybrid (Y2H), coimmunoprecipitation (Co-IP) and bimolecular fluorescence complementation (BiFC), we uncovered the presence of protein complexes consisting of isopropylmalate isomerase (IPMI) and isopropylmalate dehydrogenase (IPMDH). In addition, simultaneous decreases in both IPMI and IPMDH activities in a leuc:ipmdh1 double mutants resulted in aggregated changes of GLS profiles compared to either leuc or ipmdh1 single mutants. Although the biological importance of the formation of IPMI and IPMDH protein complexes has not been documented in any organisms, these complexes may represent a new regulatory mechanism of substrate channeling in GLS and/or leucine biosynthesis. Since genes encoding the two enzymes are widely distributed in eukaryotic and prokaryotic genomes, such complexes may have universal significance in the regulation of leucine biosynthesis.

scholarly journals Molecular characterization of clinical carbapenem-resistant Enterobacterales from Qatar

2021 ◽  
Author(s):  
Fatma Ben Abid ◽  
Clement K. M. Tsui ◽  
Yohei Doi ◽  
Anand Deshmukh ◽  
Christi L. McElheny ◽  
...  
Keyword(s):  
Common Species ◽  
Clinical Samples ◽  
Whole Genome ◽  
E Coli ◽  
Carbapenem Resistant ◽  
Genes Encoding ◽  
Encoding Genes ◽  
Sequence Types ◽  
Common Sequence

AbstractOne hundred forty-nine carbapenem-resistant Enterobacterales from clinical samples obtained between April 2014 and November 2017 were subjected to whole genome sequencing and multi-locus sequence typing. Klebsiella pneumoniae (81, 54.4%) and Escherichia coli (38, 25.5%) were the most common species. Genes encoding metallo-β-lactamases were detected in 68 (45.8%) isolates, and OXA-48-like enzymes in 60 (40.3%). blaNDM-1 (45; 30.2%) and blaOXA-48 (29; 19.5%) were the most frequent. KPC-encoding genes were identified in 5 (3.6%) isolates. Most common sequence types were E. coli ST410 (8; 21.1%) and ST38 (7; 18.4%), and K. pneumoniae ST147 (13; 16%) and ST231 (7; 8.6%).

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