scholarly journals Development of a Superior Somaclone of Rose-scented Geranium and a Protocol for Inducing Variants

HortScience ◽  
2002 ◽  
Vol 37 (4) ◽  
pp. 632-636 ◽  
Author(s):  
Ritika Gupta ◽  
S. Banerjee ◽  
G.R. Mallavarapu ◽  
S. Sharma ◽  
S.P.S. Khanuja ◽  
...  
Keyword(s):  
Essential Oil ◽  
Block Design ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Wild Type ◽  
Yield And Quality ◽  
Randomized Block Design ◽  
Half Strength ◽  
Wild Type Parent

An efficient protocol has been established for generating somaclones in the Indian rose-scented geranium Pelargonium graveolens cv. Bipuli, which yields Reunion Island-type essential oil. Murashige and Skoog's (MS) medium supplemented with 4.5 mg·L-1 BA and 1.0 mg·L-1 NAA was found optimal for induction of callus from leaf explants. Callus regenerated shoots when transferred to MS medium with 2.5 mg·L-1 BA and 0.1 mg·L-1 NAA. The regeneration percentage as well as number of shoots per cm2 of callus was greatly improved by addition of ADS at a concentration of 3.0 mg·L-1. Regenerated shoots rooted within 20 days following transfer to half-strength MS medium with 0.1 mg·L-1 NAA. Plantlets were acclimatized under glasshouse conditions with 80% to 85% survival. Randomly selected 30 individual calliclones were subjected to field trial with wild-type parent in randomized block design, replicated three times with 90% survival for two successive years. Characterization of these calliclones for essential oil yield and quality traits demonstrated induction of variability in all the characteristics examined in negative and positive directions in comparison with the wild-type parent. This screening led to the identification of somaclone B22, which out-yielded the wild-type parent as well as the rest of the somaclones. The quality of the essential oil of B22 was similar to that of the parent. Chemical names used: N6-benzyladenine (BA); naphthalene acetic acid (NAA); adenine di-sulphate (ADS).

scholarly journals Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants

2012 ◽  
Vol 81 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Jun Li ◽  
Ye Kang ◽  
Sheng Qiang ◽  
Gary Peng
Keyword(s):  
Invasive Plant ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Nodal Segments ◽  
Naphthalene Acetic Acid ◽  
Axillary Shoot ◽  
Solidago Canadensis ◽  
Ms Medium ◽  
Regeneration System ◽  
Half Strength

Goldenrod (<em>Solidago canadensis </em>L.) is an invasive plant species in many countries except North America but a cut-flower species worldwide. There is a need to generate and propagate goldenrod clones efficiently for research and commercial purposes. A callus induction and plantlet regeneration system was developed by studying the influence of explant type and different concentrations of plant growth regulators. The highest callus production from leaf segments was obtained on Murashige and Skoog’s medium (MS medium) supplemented with 1.0 mg/L naphthalene acetic acid (NAA) and 1.0 mg/L 6-benzylaminopurine (BA). Adventitious shoots could be regenerated directly from leaf explants without an intermediate callus phase with the highest shoot induction percentage of 87.2%. The largest number of adventitious shoots per leaf explant (3.2) was obtained on MS medium supplemented with 0.4 mg/L NAA and 2.0 mg/L BA. MS medium supplemented with 0.1 mg/L NAA and 1.0 mg/L BA was the best medium for axillary shoot regeneration from nodal segments. The highest root number and longest roots occurred on half-strength MS without the addition of any growth regulator. Rooted plantlets were then transferred to a soil-based growth medium, placed in a greenhouse, and acclimatized with 100% success. All surviving plants grew normally without showing any morphological varia&shy;tion when compared to those grow from seed. This regeneration protocol may be used to produce certain biotypes of goldenrod suitable for genetic transformation rapid propagation of goldenrod for commercial purposes or for screening fungi and toxins as potential biocontrol agents against this weed.

scholarly journals STABILITAS HASIL LIMA NOMOR HARAPAN KENCUR

2020 ◽  
Vol 12 (4) ◽  
pp. 140
Author(s):  
OTIH ROSTIANA ◽  
WAWAN HARYUDIN ◽  
ROSITA SMD SMD
Keyword(s):  
Essential Oil ◽  
Oil Content ◽  
Block Design ◽  
Fertilizer Application ◽  
Plant Spacing ◽  
Materia Medica ◽  
Plot Size ◽  
Yield And Quality ◽  
Kaempferia Galanga ◽  
Randomized Block Design

ABSTRAK<br />Tujuan pemuliaan kencur diarahkan untuk meningkatkan produksi<br />rimpang dan kandungan mutu utama yang sesuai dengan standar yang<br />ditetapkan oleh Materia Medika Indonesia, dalam upaya meningkatkan<br />pendapatan usahatani. Hasil seleksi berdasarkan produksi dan mutu<br />rimpang terhadap aksesi plasma nutfah yang terkumpul, terpilih 5 nomor<br />dengan rata-rata produksi rimpang &gt; 40 g/rumpun dan kadar minyak atsiri<br />&gt; 1,5%, yaitu V1, V2, V3, V4 dan V5. Kelima nomor diuji multilokasi di 5<br />lokasi pada 2 musim tanam (tahun 2002/2003 dan 2003/2004). Kelima<br />lokasi tersebut adalah: Cileungsi (80 m dpl.) dan Cijeruk (650 m dpl.)<br />(Bogor), Subang (80 m dpl), Sumedang (550 m dpl) dan Sukamulya (350<br />m dpl.) (Sukabumi). Pengujian menggunakan rancangan acak kelompok<br />dengan 6 ulangan, jarak tanam 20 x 20 cm, ukuran petak 4 x 1 m 2 ,<br />populasi tanaman per petak 100. Dari hasil pengujian diperoleh nomor<br />yang menghasilkan rata-rata bobot rimpang tertinggi yaitu V4 (53,58<br />g/rumpun atau setara dengan 10,7 ton/ha), rata-rata kadar minyak atsiri<br />tertinggi (6,64%) dan beradaptasi secara spesifik pada lingkungan tumbuh<br />yang sama dengan lokasi penanaman di Cijeruk, Sumedang dan Suka-<br />mulya. Sedangkan V2 dengan nilai diameter rimpang terbesar (2,089 cm),<br />stabil di 4 lokasi pengujian, responsif terhadap pemupukan, serta V3<br />dengan rata-rata produksi rimpang 51,98 g/rumpun atau setara dengan 10,4<br />ton/ha, beradaptasi secara spesifik pada lingkungan tumbuh yang sama<br />dengan lokasi penanaman kencur di Cileungsi, Cijeruk dan lokasi asalnya<br />di Sumedang.<br />Kata kunci : Kencur, Kaempferia galanga L., varietas unggul, stabilitas<br />hasil, spesifik lokasi, Jawa Barat<br />ABSTRACT<br />Stability of five promissing Galanga lines production<br />Breeding of Galanga was focused on the improvement of rhizome<br />yield and quality based on the Indonesian Materia Medica Standard, to<br />increase farmers’ income. Based on the selection on rhizome yield and<br />quality, 5 accession numbers were selected as promising lines for their<br />high rhizome yield (&gt;40 g/tiller) and essential oil contents (&gt; 1.5%), e.g.<br />V1, V2, V3, V4 and V5. All numbers were subjected to multilocation tests<br />at 5 locations, e.g. Cileungsi (80 m asl) and Cijeruk (650 m asl) (Bogor),<br />Subang (80 m asl), Sumedang (550 m asl) and Sukamulya (350 m asl)<br />(Sukabumi), for 2 planting-seasons (2002/2003 and 2003/2004). Experi-<br />ments were arranged in a randomized-block design, with 6 replications,<br />plant spacing 20 cm x 20 cm, plot size of 4 x 1 m2, and population 100<br />plants/plot. The research results showed that the highest average of<br />rhizome yield was performed by V4 (53.58 g/tiller, equal to 10.7 t/ha),<br />with essential oil content 6.64% and specifically adaptable to the same<br />agro-climate as the environment conditions at Cijeruk (Bogor), Sumedang<br />and Sukamulya (Sukabumi). Meanwhile, V2 with the highest diameter of<br />rhizome (2,089 cm), stable at 4 out of 5 locations and responsive to<br />fertilizer application. On the other hand, V3 with the average of rhizome<br />yield 51.98 g/tiller, equal to 10.4 t/ha, specifically adaptable to the agro-<br />climate similar to the environment conditions at Cileungsi, Cijeruk<br />(Bogor) and its native, Sumedang.<br />Key words: Kaempferia galanga L., superior variety, yield stability,<br />specific location, West Java

scholarly journals SERAPAN HARA N, P, K PADA TUJUH NOMOR HARAPAN SERAI DAPUR PADA TANAH LASOTOL / The Nutrient Uptake of N, P, and K of Seven Promising Numbers of Lemongrass in Latosol Soil

2017 ◽  
Vol 23 (2) ◽  
pp. 105
Author(s):  
Octivia Trisilawati ◽  
Deliah Seswita ◽  
M. Syakir
Keyword(s):  
Essential Oil ◽  
Nutrient Uptake ◽  
Oil Content ◽  
Block Design ◽  
P Uptake ◽  
Leaf Length ◽  
Oil Yield ◽  
Cymbopogon Citratus ◽  
Yield And Quality ◽  
Randomized Block Design

<p>The constrain of lemongrass cultivation is crop nutrient requirement does not estimate yet as a reference for determining the dosage of fertilizer needed to produce good yield and quality. The study aims to determine the NPK uptake of seven lemongrass promising numbers grown in latosol soil in Cibinong research garden, Bogor, namely: Cyci 0003, 0004, 0006, 0009, 0012, 0018 and local. Seven lemongrass promising numbers were from Boyolali, Yogyakarta, Cipatat, Cisaroni, East Nusa Tenggara, Bogor, and Cibinong which had been characterized. The design used was randomized block design with four replications and 25 plants per plots. The parameters observed were number of tillers, plant height, leaf length and width, stem diameter weight per clump, oil yield and quality, and chlorophyll content. The results showed that there were differences of nutrient uptake pattern, as well as the amount of fertilizer requirement N, P and K on all seven promising numbers of lemongrass. Local and Cyci 0003 had highest oil and sitral production compared to other lemongrass promising numbers. To generate the essential oil yield 81,39 kg ha-1 and production of citral 14,25 tonnes ha-1, Cyci 0003 absorbed 284,65 kg Urea, 49,15 kg SP-36, and 308,95 kg KCl ha-1, while local Cyci absorbed 230,29 kg Urea, 49,97 kg SP-36, and 161,8 kg KCl ha-1 to produce 97,94 kg essential oils yield ha-1 and 16,01 tonnes citral ha-1. Essential oil content of Lemongrass increased with increasing uptake of N and P, and citral oil content increased with increasing nutrient P uptake. Local Cyci promising number was relatively efficient in N, P, and K nutrient absorption.</p><p>Keywords: Cymbopogon citratus Stapf., promising number, yield, quality, the uptake of N, P, and K.</p><p> </p><p><strong>Abstrak</strong></p><p>Salah satu kendala dalam pengembangan budidaya serai dapur adalah belum ada perhitungan kebutuhan hara tanaman secara riil sebagai acuan dalam menentukan dosis pupuk yang dibutuhkan untuk menghasilkan produksi dan mutu terna yang baik. Penelitian bertujuan untuk mengetahui serapan hara N, P, dan K dari tujuh nomor harapan serai dapur yang ditanam di tanah latosol. Tujuh nomor harapan serai dapur yang telah dikarakterisasi yaitu Cyci 0003, Cyci 0004, Cyci 0006, Cyci 0009, Cyci 0012, Cyci 0018 dan Cyci lokal berasal dari Boyolali, Yogyakarta, Cipatat, Cisaroni, NTT, Bogor, dan Cibinong ditanam di kebun percobaan Cibinong, Bogor. Penelitian menggunakan Rancangan Acak Kelompok, dengan 4 ulangan dan jumlah tanaman sebanyak 25 per petak. Parameter yang diamati meliputi jumlah anakan, tinggi tanaman, panjang dan lebar daun, diameter batang, bobot kering terna per rumpun, kadar minyak, mutu minyak, serta kandungan klorofil. Hasil penelitian menunjukkan bahwa terdapat perbedaan pola serapan hara, jumlah serta kebutuhan pupuk N, P, dan K dari ke tujuh nomor harapan serai dapur. Cyci lokal dan Cyci 0003 menghasilkan minyak atsiri dan sitral tertinggi. Untuk menghasilkan minyak atsiri 81,39 kg ha-1 dan sitral 14,25 t ha-1, Cyci 0003 menyerap 284,65 kg Urea, 49,15 kg SP-36, dan 308,95 kg KCl ha-1, sedangkan untuk menghasilkan minyak atsiri 97,94 kg ha-1 dan sitral 16,01 ton ha-1, Cyci lokal menyerap 230,29 kg Urea, 49,97 kg SP-36 dan 161,8 kg KCl ha-1. Kadar minyak atsiri serai dapur meningkat sejalan dengan peningkatan serapan hara N dan P, dan kadar minyak sitral meningkat seiring dengan peningkatan serapan hara P. Cyci lokal merupakan nomor harapan serai dapur yang relatif efisien dalam penyerapan hara N, P, dan K.</p><p>Kata kunci: Cymbopogon citratus Stapf, nomor harapan, produksi, mutu, serapan hara N, P dan K.</p><p> </p>

Effect of Integrated Nutrient Management on Yield and Quality of Garlic cv. Yamuna Safed-3

2020 ◽  
Vol 7 (04) ◽  
Author(s):  
A B Priyanshu ◽  
M K Singh ◽  
Mukesh Kumar ◽  
Vipin Kumar ◽  
Sunil Malik ◽  
...  
Keyword(s):  
Nutrient Management ◽  
Block Design ◽  
Quality Parameters ◽  
Organic Fertilizers ◽  
Research Centre ◽  
Yield And Quality ◽  
Randomized Block Design ◽  
The Impact ◽  
Horticultural Research

An experiment was conducted at Horticultural Research Centre, SVP University of Agriculture and Technology, Meerut (UP) during Rabi season of 2018-19 to assess the impact of different INM doses on yield and quality parameters of garlic. A total of ten treatments consisting of combinations of inorganic fertilizers, organic fertilizers and bio-fertilizers like T1- (Control), T2RDF (100:50:50 kg NPK ha-1), T3-RDF + 20 kg sulphur + FYM 20 ton ha-1, T4- RDF + 20 kg sulphur + VC 4 ton ha-1, T5- 75% RDF + 40 kg sulphur + 5 ton FYM ha-1+ PSB 5 kg ha-1, T6-75% RDF + 40 kg sulphur + 2 ton VC + Azotobacter 5 kg ha-1, T7- 75% RDF + 40 kg sulphur + FYM 3 ton + VC 1 ton+ PSB 5 kg + Azotobacter 5kg ha-1, T8- 50% RDF + 40 kg sulphur + FYM 5 ton + VC2 ton + PSB 5 kg ha-1, T9- 50% RDF + 40 kg sulphur + FYM5 ton + VC 2 ton+ Azotobacter 5 kg ha-1and T10- 50% RDF + 40 kg sulphur + FYM 5 ton + VC2 ton + PSB 5 kg + Azotobacter 5 kg ha-1 were used in Randomized Block Design and replicated thrice. Out of these an application of T7 (75% RDF + 40 kg sulphur + FYM3 ton + VC 1 ton ha-1 + PSB 5 kg + Azotobacter 5 kg ha-1) was found to be significantly superior in term of yield and attributing parameters of garlic.

scholarly journals IN VITRO REGENERATION AND MASS PROPAGATION OF AZIMA TETRACANTHA [LAM.] FROM THE LEAF EXPLANTS THROUGH CALLUS CULTURE

2017 ◽  
Vol 4 (2) ◽  
pp. 52-56
Author(s):  
Mallika Devi T
Keyword(s):  
Callus Induction ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Shoot Formation ◽  
Ms Medium ◽  
Apical Leaf ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
Azima Tetracantha

In the present study the protocol for callus induction and regeneration in Azima tetracantha has been developed in culture medium. The young apical leaf explants were used for callus induction on MS medium containing BAP and NAA at 1.0 and 0.4mgl-1 respectively showed maximum callus induction (73%). The amount of callus responded for shoot formation (74%) was obtained in the MS medium containing BAP (1.5 mgl-1) and NAA (0.3mgl-1).The elongated shoots were rooted on half strength medium supplemented with IBA (1.5 mgl-1) and Kn (0.4 mgl-1) for shoots rooted. Regenerated plantlets were successfully acclimatized and hardened off inside the culture and then transferred to green house with better survival rate.

scholarly journals Melon fruit yield and quality as a function of doses and times of biostimulant application

2020 ◽  
Vol 9 (7) ◽  
pp. e130973911
Author(s):  
Roberto Cleiton Fernandes de Queiroga ◽  
Zaqueu Lopes da Silva ◽  
Odair Honorato Oliveira de ◽  
Elidayane da Nóbrega Santos ◽  
Higínio Luan Oliveira Silva ◽  
...  
Keyword(s):  
Block Design ◽  
Fruit Production ◽  
Soluble Solids ◽  
Application Time ◽  
Time Of Application ◽  
Yield And Quality ◽  
Randomized Block Design ◽  
Semi Arid Region ◽  
Fruit Mass

The objective of this study was to evaluate the productivity and quality of melon fruits as a function of the dose and time of application of biostimulant in the conditions of the semi-arid region of Paraíba. The experiment was carried out at the Federal University of Campina Grande, campus of Pombal - PB, Brazil, in a randomized block design in a 4 x 5 split plot scheme, with doses of biostimulant (0; 0.5; 1.0; 1, 5 and 2.0 L ha-1) and in the subset of the biostimulant application times (15; 20; 25 and 30 days before harvest - DAC), in four replications. Characteristics related to fruit production and quality were evaluated. There was no interaction between the factors of dose and application time of the biostimulant in any of the evaluated characteristics. Thus, the highest estimated values of number of fruits per plant, fruit mass and total melon production were obtained with the application of doses ranging from 0.9 to 1.5 L ha-1 and at the time of application it varied from 22,5 to 23.6 DAC. The content of soluble solids increased 5.5% when the biostimulant dose of 2.0 L ha-1 was used and 4.4% when the product was applied 15 days before harvest.

scholarly journals In Vitro Propagation of Digitalis trojana Ivanina., an Endemic Medicinal Plant of Turkey

2020 ◽  
Author(s):  
Nurşen Çördük ◽  
Cüneyt Aki
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
In Vitro Propagation ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Northwestern Turkey ◽  
Helen Of Troy ◽  
Endemic Medicinal Plant

Digitalis trojana Ivanina is a member of the Plantaginaceae family and known by its common name, Helen of Troy foxglove. It is perennial endemic to Çanakkale and Balıkesir, northwestern Turkey. In order to develop an efficient shoot regeneration protocol, the leaf explants of D. trojana were cultured on Murashige and Skoog (MS) medium containing 6-benzyl adenine (0.1, 0.5, 1.0, 3.0, 5.0 mg/L) and α-naphthalene acetic acid (0.1, 0.5, 1.0 mg/L), 3% (w/v) sucrose and 0.8% (w/v) agar. The highest number of regenerated shoots was obtained from leaf explants that were cultured on MS medium with 3.0 mg/L BA+0.1 mg/L NAA. Regenerated shoots were rooted on MS medium without plant growth regulators. Rooted plants (2–3 cm) were separately transferred to pots containing a mixture of peat and perlite (2:1 v/v) and acclimatized successfully in a growth chamber.

scholarly journals Comparative Effect of Different Treaments in Field Pea (Pisum sativum L.)

2021 ◽  
pp. 221-225
Author(s):  
Tharuna Sree ◽  
Abhinav Dayal ◽  
Prashanth Kumar Rai
Keyword(s):  
Gibberellic Acid ◽  
Block Design ◽  
Uttar Pradesh ◽  
Field Pea ◽  
Growth And Yield ◽  
Naphthalene Acetic Acid ◽  
Comparative Effect ◽  
Randomized Block Design ◽  
Biological Yield ◽  
Neem Leaf Extract

A field study was conducted to investigate the various treatments that effect on growth and yield of field pea in RBD (Randomized block design) at Sam Higginbottom University of Agriculture Technology and Science, Prayagraj, Uttar Pradesh during march to May in 2020. The trail consists of 13 treatments combinations. The field pea varieties were used were IPF429. The treatments included T0- control, T1,T2,T3,-Gibberellic acid, T4,T5,T6- Neem leaf extract, T7,T8,T9-ZnSo4, T10T11T12- Naphthalene acetic acid(NAA). All Ten parameters treated with Ga3 shows good results in Yield and shows maximum in field emergence, plant height, Days to 50% flowering, Number of pods, Seed yield per plot, Biological Yield and Harvest index. T0 (Un primed) shows lowest of all treatments. Hence, priming with Gibberellic acid could recommended for pre sowing treatment for field pea.

scholarly journals Calli induction in leaf explants of coffee elite genotypes

2011 ◽  
Vol 41 (3) ◽  
pp. 384-389 ◽  
Author(s):  
Juliana Costa de Rezende ◽  
Carlos Henrique Siqueira de Carvalho ◽  
Moacir Pasqual ◽  
Ana Carolina Ramia Santos ◽  
Stephan Malfitano de Carvalho
Keyword(s):  
Coffea Arabica ◽  
Block Design ◽  
Leaf Explants ◽  
Production Potential ◽  
Embryogenic Calli ◽  
The Third ◽  
Randomized Block Design ◽  
Indirect Somatic Embryogenesis ◽  
Growth Room ◽  
Opposite Behavior

Three experiments were carried out with the objective of achieving high effectiveness in calli induction from high heterozygosis leaf explants of Coffea arabica through indirect somatic embryogenesis. A randomized-block design in a 2x5 factorial arrangement made up of two media [BOXTEL & BERTHOULY (1996) and TEIXEIRA et al. (2004)] and five C. arabica genotypes were used in the first experiment. In the second experiment the embryogenic calli production potential was evaluated in ten genotypes. Each of them was considered as a treatment. In the third experiment the variations in both 2.4-D (2.5 e 20µM) and 2-iP (2.5 e 20µM) concentrations in TEIXEIRA et al. (2004) medium and secondary media were evaluated. Crops were kept in a growth room under darkness, at 25±2oC. The medium described by TEIXEIRA et al (2004) was found to be superior when compared to that described by BOXTEL & BERTHOULY (1996) in the 2.2 and 7.2 genotypes. An opposite behavior was noticed in 4.2 genotype, that is, BOXTEL & BERTHOULY (1996) had medium superiority. Both 3.0 and 5.0 genotypes had the same behavior in both media studied, which shows that the somatic embryo production depends on the genotype. Calli induction depends on the 2-iP and 2.4 D ratio. The 20.0µM of 2.4-D and 20.0µM of 2-iP combination caused the highest embryogenic calli induction rate.

scholarly journals Effects of fertilizer and plant density on yield and quality of anise (Pimpinella anisum L.)

2013 ◽  
Vol 58 (3) ◽  
pp. 209-215
Author(s):  
Mahdi Faravani ◽  
Behjat Salari ◽  
Mostafa Heidari ◽  
Mohammad Kashki ◽  
Barat Gholami
Keyword(s):  
Essential Oil ◽  
Plant Density ◽  
Block Design ◽  
Organic Fertilizer ◽  
Canopy Cover ◽  
Mineral Fertilizer ◽  
Yield And Quality ◽  
Plant Densities ◽  
Biological Yield ◽  
Lateral Branches

In order to understand the effect of organic fertilizer on yield of anise, an experiment was conducted in the form of split-plot in randomized complete block design with three replications in Mashhad, Khorasan Agriculture and Natural Resource Research Center. Four treatments of fertilization: the control, vermicompost - 5 t/ha, cow manure - 25 t/ha, and mineral fertilizer (NPK) - 60 kg/ha (the same rate of each nutrient) were applied as the main factor. The second factor was plant density, applied at three levels: 17, 25, and 50 plants/m2. The results showed a significant effect of fertilizer on the number of umbels per plant, number of umbellets per umbel and canopy cover. Plant density had a significant effect on grain yield, biological yield, the number of lateral branches, essential oil percentage and yield of essential oil. Seed and essential oil yield were the highest in the case of the application of vermicompost and plant densities of 50 and 25 plants/m2 respectively.

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