A Sequence-amplified Characterized Region Marker for a Single, Dominant Gene in Melon PI 134198 that Confers Resistance to a Unique Race of Podosphaera xanthii in China

Melon production in China is threatened by cucurbit powdery mildew incited by Podosphaera xanthii, which can cause severe yield losses. Disease reactions of 10 melon P. xanthii race differentials indicated the presence of a unique race of P. xanthii, denoted pxCh1, in the Shanghai area. Resistance to P. xanthii race pxCh1 in melon PI 134198, which originated from India, was controlled by a single, dominant gene, designated Pm-8, in a cross with susceptible melon inbred S717. A search for a molecular marker linked to Pm-8 was initiated using the same cross. Bulk segregant analysis of their F2 progeny revealed one sequence-related amplified polymorphism (SRAP) marker of 148 bp linked to Pm-8, at 3.9 cM. The SRAP segment was recovered, cloned, and sequenced and converted into a sequence-amplified characterized region marker for marker-assisted selection of Pm-8 in breeding programs to develop melon cultivars resistant to P. xanthii race pxCh1.

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Mapping QTL for spike fertility and related traits in two doubled haploid wheat (Triticum aestivum L.) populations

BMC Plant Biology ◽

10.1186/s12870-021-03061-y ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Nicole Pretini ◽

Leonardo S. Vanzetti ◽

Ignacio I. Terrile ◽

Guillermo Donaire ◽

Fernanda G. González

Keyword(s):

Doubled Haploid ◽

Marker Assisted Selection ◽

Triticum Aestivum L ◽

Physiological Model ◽

Yield Potential ◽

Breeding Programs ◽

Per Se ◽

Genomic Regions ◽

Fertility Traits ◽

Selection Of

Abstract Background In breeding programs, the selection of cultivars with the highest yield potential consisted in the selection of the yield per se, which resulted in cultivars with higher grains per spike (GN) and occasionally increased grain weight (GW) (main numerical components of the yield). In this study, quantitative trait loci (QTL) for GW, GN and spike fertility traits related to GN determination were mapped using two doubled haploid (DH) populations (Baguette Premium 11 × BioINTA 2002 and Baguette 19 × BioINTA 2002). Results In total 305 QTL were identified for 14 traits, out of which 12 QTL were identified in more than three environments and explained more than 10% of the phenotypic variation in at least one environment. Eight hotspot regions were detected on chromosomes 1A, 2B, 3A, 5A, 5B, 7A and 7B in which at least two major and stable QTL sheared confidence intervals. QTL on two of these regions (R5A.1 and R5A.2) have previously been described, but the other six regions are novel. Conclusions Based on the pleiotropic analysis within a robust physiological model we conclude that two hotspot genomic regions (R5A.1 and R5A.2) together with the QGW.perg-6B are of high relevance to be used in marker assisted selection in order to improve the spike yield potential. All the QTL identified for the spike related traits are the first step to search for their candidate genes, which will allow their better manipulation in the future.

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Selection of powdery mildew resistant and susceptible grapevine genotypes with molecular markers

Acta Agraria Debreceniensis ◽

10.34101/actaagrar/27/3110 ◽

2007 ◽

pp. 100-104

Author(s):

Stella Molnár ◽

Zsuzsanna Galbács ◽

Gábor Halász ◽

Sarolta Hoffmann ◽

Anikó Veres ◽

...

Keyword(s):

Molecular Markers ◽

Powdery Mildew ◽

Marker Assisted Selection ◽

Powdery Mildew Resistance ◽

Resistance Breeding ◽

Breeding Programs ◽

Pcr Rflp ◽

Muscadinia Rotundifolia ◽

Selection Of ◽

Grape Breeding

Incorporation of competitive quality and resistance against the most important fungal diseases (powdery and downy mildew) in a cultivar is one of the most important aims of grapevine breeding. In the 20th century, the most advanced results in grapevine resistance breeding were achieved by French researchers. They used resistant cultivars in more than 30% of their growing areas. In these varieties, North American wild Vitisspecies were the resistance gene sources. The discovery of immunity-like resistance of Muscadinia rotundifolia opened new perspectives in resistance breeding. M. rotundifolia harbours a dominant powdery mildew gene, providing resistance in highquality cultivars after back-crosses with V. vinifera varieties. M. rotundifolia has been involved in the Hungarian grape breeding programs since 1996, thanks to a French-Hungarian variety exchange. In addition to traditional selection methods, application of MAS (Marker Assisted Selection) based on various types ofmolecular markers, can provide additional tools for these efforts. Run1 locus, responsible for powdery mildew resistance, was identified in Muscadinia rotundifolia. Molecular markers closely linked to this locus are very significant in screening progenies deriving from M. rotundifolia and V. vinifera crosses, making possible the discrimination between resistant and susceptible genotypes at DNA level. In our analyses BC5 progeny of {(M. rotundifola×V. vinifera) BC4}×Cardinal (V. vinifera) tested for powdery symptoms were analysed with PCR-RFLP (GLP1- 12P1P3) and microsatellite markers (VMC4f3.1, VMC8g9). Our results proved the applicability of the linked markers and reliability of marker assisted selection.

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Indirect selection of Cre1 gene in winter wheat populations

Archives of Biological Sciences ◽

10.2298/abs1101049c ◽

2011 ◽

Vol 63 (1) ◽

pp. 49-53 ◽

Cited By ~ 2

Author(s):

M. Çalişkan ◽

S. Uranbey ◽

J. Nicol ◽

T. Akar ◽

H. Elekçioğlu ◽

...

Keyword(s):

Winter Wheat ◽

Production Systems ◽

Early Stage ◽

Dominant Gene ◽

Indirect Selection ◽

Central Anatolia ◽

Yield Losses ◽

Heterodera Filipjevi ◽

Species Being ◽

Selection Of

The nematodes are important biotic constraint in rain-fed wheat production systems. In Turkey, they is found in 75.0% of soil samples in Central Anatolia with the dominant species being Heterodera filipjevi. Yield losses for winter wheat in rain-fed environments are documented between 27.0-46.0%. A single dominant gene for resistance to H. avenae, designated as Cre1, was assessed in Turkey. It was also found to be effective to Heterodera filipjevi. In this research, a STS based Cre1 marker was applied in a number of segregating wheat populations from F1 to F4 to discriminate Cre1-positive lines among the wheat populations. Results clearly indicated that Marker Assisted Selection (MAS) is functioning effectively, with recovery of Cre1 positive lines up to 88.0 % depending on the cross in early stage of breeding.

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Molecular mapping of the C locus for presence of pungency in Capsicum

Genome ◽

10.1139/g02-031 ◽

2002 ◽

Vol 45 (4) ◽

pp. 702-705 ◽

Cited By ~ 53

Author(s):

Eyal Blum ◽

Kede Liu ◽

Michael Mazourek ◽

Eun Young Yoo ◽

Molly Jahn ◽

...

Keyword(s):

High Throughput Screening ◽

Marker Assisted Selection ◽

Molecular Mapping ◽

Dominant Gene ◽

Wild Accession ◽

Rflp Markers ◽

Chromosome 2 ◽

Breeding Programs ◽

Unique Character ◽

Capsicum Spp

Pungency owing to the presence of capsaicinoids is a unique character of pepper (Capsicum spp.). Capsaicinoids are produced in the placenta and it has long been known that a single dominant gene, C, is required for pungent genotypes to produce capsaicinoids. We mapped C to pepper chromosome 2 in a cross between a pungent Capsicum frutescens wild accession and a non-pungent Capsicum annuum bell pepper. This position confirmed results from earlier studies. The RFLP marker TG 205 cosegregated with C and two additional RFLP markers were also located within 1 cM. The recessive allele at the C locus is used in breeding programs around the world focused on very diverse germplasm, hence any of these tightly linked markers may be of value as potential sources of useful markers for marker-assisted selection. To demonstrate this point, we developed a PCR-based CAPS (cleaved amplified polymorphic sequence) marker linked to C using the sequence of the Capsicum fibrillin gene located 0.4 cM from C. The use of molecular markers for high-throughput screening for the c allele in pepper breeding programs is discussed.Key words: pepper, pungency, marker-assisted selection.

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QTL detection of yield-related traits of cashew

Cropp Breeding and Applied Biotechnology ◽

10.1590/s1984-70332012000100008 ◽

2012 ◽

Vol 12 (1) ◽

pp. 60-66 ◽

Cited By ~ 5

Author(s):

José Jaime Vasconcelos Cavalcanti ◽

Francisco Herbeth Costa dos Santos ◽

Fanuel Pereira da Silva ◽

Cássia Renata Pinheiro

Keyword(s):

Quantitative Trait ◽

Marker Assisted Selection ◽

Interval Mapping ◽

Phenotypic Variance ◽

Qtl Detection ◽

Total Phenotypic Variance ◽

Breeding Programs ◽

Multiple Qtl ◽

Male Flowers ◽

Selection Of

The identification of quantitative trait loci (QTL) and marker-assisted selection with a view to breeding programs have aroused great interest, including for cashew improvement. This study identified QTL for yield-related traits: nut weight, male and hermaphrodite flowers. The traits were evaluated in 71 F1 genotypes of the cross CCP 1001 x CP 96. The methods of interval mapping and multiple QTL mapping were applied to identify QTL. Eleven QTL were detected: three for nut weight, four for male flowers and four for hermaphrodite flowers. The QTL accounted for 3.79 to 12.98 % of the total phenotypic variance and had phenotypic effects of -31.81 to 34.25 %. The potential for marker-assisted selection of the QTL hf-2f and hf-3m is great and the phenotypic effects and percentage of phenotypic variation higher than of the others.

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The identification of random amplified polymorphic DNA markers for daylength insensitivity in oat

Genome ◽

10.1139/g94-130 ◽

1994 ◽

Vol 37 (6) ◽

pp. 910-914 ◽

Cited By ~ 14

Author(s):

Charlene P. Wight ◽

Greg A. Penner ◽

Louise S. O'Donoughue ◽

Vernon D. Burrows ◽

Stephen J. Molnar ◽

...

Keyword(s):

Avena Sativa ◽

Dna Markers ◽

Field Data ◽

Bulk Segregant Analysis ◽

Segregation Analysis ◽

Random Amplified Polymorphic Dna ◽

Dominant Gene ◽

Dominant Allele ◽

Single Dominant Gene ◽

Polymorphic Bands

Daylength insensitive accessions of Avena sativa L. are being used to develop cultivars that will flower normally when grown under short or long photoperiods. Field data indicate that the insensitivity trait is under the control of a single dominant gene, designated Di1. The random amplified polymorphic DNA (RAPD) technique and bulk segregant analysis of daylength sensitive and insensitive plants were used to find markers for this gene. Five of 200 random decamer primers tested produced polymorphic bands, which were shown to be linked to the trait using 30 homozygous insensitive and 30 homozygous sensitive F3 individuals. Three of the markers produced a band in the presence of the dominant allele, and two in its absence. Segregation analysis showed that markers 221 and 136 could be mapped to within 9.8 ± 4.6 and 13.9 ± 5.4 cM of the trait, respectively; that is, close enough to be useful in a breeding program. A study of different cultivars suggested that the band produced by primer 136 is actually the more closely linked marker and the only one present in the original Di1 gene donor CAV2700. The possibility of using both markers in populations derived from different cultivars is discussed.Key words: RAPD, molecular marker, oat, daylength insensitivity, photoperiod, bulk segregant analysis.

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Inheritance and Expressivity of Neoplasm Trait in Crosses between the Domestic Pea (Pisum sativum subsp. sativum) and Tall Wild Pea (Pisum sativum subsp. elatius)

Agronomy ◽

10.3390/agronomy10121869 ◽

2020 ◽

Vol 10 (12) ◽

pp. 1869 ◽

Cited By ~ 1

Author(s):

Hatice Sari ◽

Duygu Sari ◽

Tuba Eker ◽

Bilal Aydinoglu ◽

Huseyin Canci ◽

...

Keyword(s):

Pisum Sativum ◽

Negative Selection ◽

Uv Light ◽

Dominant Gene ◽

Single Dominant Gene ◽

Meristematic Tissue ◽

Breeding Programs ◽

Pisum Sativum L ◽

Natural Infestation ◽

Bruchus Pisorum

The Neoplasm trait in pea pods is reported to be due to the lack of ultraviolet (UV) light in glasshouse conditions or in response to pea weevil (Bruchus pisorum L.) damage. This pod deformation arises from the growth of non-meristematic tissue on pods of domesticated peas (Pisum sativum L. subsp. sativum). Neither expressivity, nor the effect of pea weevil on neoplasm in the tall wild pea (P. sativum L. subsp. elatius (M. Bieb.) Asch. & Graebn.), have been adequately studied. We aimed to study the expression and inheritance of neoplasm in the tall wild pea and crosses between domesticated and tall wild peas grown in the glasshouse (without pea weevils) and in the field (with pea weevils) under natural infestation conditions. Neoplasm was found in all pods in tall wild peas when grown in the glasshouse, while it was not detected on pods of field-grown plants despite heavy pea weevil damage. In inter-subspecific crosses between P. sativum subsp. sativum and P. sativum subsp. elatius, all F1 plants had neoplastic pods, and the F2 populations segregated in a good fit ratio of 3 (neoplasm): 1 (free from neoplasm) under glasshouse conditions, which suggests that neoplasm on pods of the tall wild pea was controlled by a single dominant gene. Expressivity of neoplasm in the progeny differed from parent to parent used in inter-subspecific crosses. There was no relationship between neoplasm and damage by pea weevil under heavy insect epidemics under field conditions. The neoplasm occurring under glasshouse conditions may be due to one or to a combination of environmental factors. Since wild peas are useful genetic resources for breeding programs aiming at fresh pea production that could be utilized under glasshouse conditions, negative selection could be considered in segregating populations.

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Identification of Co-1 Anthracnose Resistance and Linked Molecular Markers in Common Bean Line A193

Plant Disease ◽

10.1094/pdis.2001.85.3.252 ◽

2001 ◽

Vol 85 (3) ◽

pp. 252-255 ◽

Cited By ~ 15

Author(s):

Azucena Mendoza ◽

Fernando Hernández ◽

Sanjuana Hernández ◽

Daniel Ruíz ◽

Octavio Martínez de la Vega ◽

...

Keyword(s):

Length Polymorphism ◽

Fragment Length ◽

Marker Assisted Selection ◽

Fragment Length Polymorphism ◽

Random Amplified Polymorphic Dna ◽

Dominant Gene ◽

Colletotrichum Lindemuthianum ◽

Single Dominant Gene ◽

Selection For ◽

Race 2

Phaseolus vulgaris line A193 has been shown to be widely resistant to Colletotrichum lindemuthianum, including race 1472, one of the most virulent races of C. lindemuthianum characterized. Resistance to C. lindemuthianum race 1472 in P. vulgaris line A193 was investigated in segregating F2 and F2.3 populations from a cross between A193 and Flor de Mayo Bajio (a commercial cultivar highly susceptible to C. lindemuthianum). Resistance to 1472 in A193 was determined to be conditioned by a single dominant gene. Inoculation of crosses between A193 and cultivars Michigan Dark Red Kidney and Perry Marrow with race 1472 suggest that resistance in A193 is conditioned by the Co-1 gene. Inoculation of the cross A193 × Perry Marrow with C. lindemuthianum race 2, demonstrated that resistance to race 2 in Perry Marrow is also conditioned by a single dominant gene and is distinct to resistance in A193 or Michigan Dark Red Kidney. Three AFLP markers (ECAG/MACC-1, EACA/MAGA-2, EAGG/MAAC-8) linked in repulsion to the Co-1 locus were identified by screening the A193 × Flor de Mayo F2 population with 314 random amplified polymorphic DNA, amplified fragment length polymorphism, and restriction fragment length polymorphism markers. The two most closely linked markers should be useful in marker-assisted selection for Co-1.

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Mapping of resistance genes to races 1, 3 and 5 of Podosphaera xanthii in melon PI 414723

Cropp Breeding and Applied Biotechnology ◽

10.1590/s1984-70332013000400005 ◽

2013 ◽

Vol 13 (4) ◽

pp. 349-355 ◽

Cited By ~ 10

Author(s):

Ana Carolina Fazza ◽

Leandro José Dallagnol ◽

Ana Cristina Fazza ◽

Carolina C. Monteiro ◽

Bruno Marco de Lima ◽

...

Keyword(s):

Linkage Group ◽

Genetic Map ◽

Resistance Genes ◽

Segregation Analysis ◽

Specific Resistance ◽

Dominant Gene ◽

Inheritance Of Resistance ◽

Podosphaera Xanthii ◽

Single Dominant Gene ◽

First Time

The fungus Podosphaera xanthii affects melon crops and presents several races controlled by race-specific resistance genes. The accession PI 414723 is resistant to races 1, 3 and 5 and it is a suitable source of resistance genes. The inheritance of resistance to these races was analyzed on 87 F2 plants from the cross of PI 414723 × Védrantais, and resistance to all three races could be explained by the segregation of a single dominant gene, although a digenic model could also be accepted. A genetic map was assembled with 206 markers, and co-segregation analysis of resistance phenotypes indicated the existence of two linked loci in linkage group II, one conferring resistance to races 1 and 5 (denominated Pm-x1,5), and the second to race 3 (denominated Pm-x3), located 5.1 cM apart. This study reports for the first time the existence of Pm-x3 and the genetic locations of these resistance genes from PI 414723.

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Gen dan QTL Pengendali Toleransi Tanaman terhadap Keracunan Aluminium dan Aplikasinya untuk Pemuliaan Tanaman di Indonesia

Jurnal AgroBiogen ◽

10.21082/jbio.v11n3.2015.p111-124 ◽

2016 ◽

Vol 11 (3) ◽

pp. 111

Author(s):

I Made Tasma

Keyword(s):

Dominant Gene ◽

Al Toxicity ◽

Breeding Program ◽

Genetic Maps ◽

Single Dominant Gene ◽

Breeding Methods ◽

Al Tolerance ◽

Crop Species ◽

Breeding Programs ◽

Genomic Technologies

Genetic knowledge of loci controlling Al toxicity tolerance is the key for a successful breeding program in developing Al tolerant cultivars. Tolerance level of crop plants to Al toxicity is genetically controlled. The gene inheritance pattern is mainly resulted from intensive studies of cereal crops, such as wheat, sorghum, maize, and rice. The trait can be controlled by a single dominant gene, a single dominant gene with many alleles, a pair of dominant genes, or by many genes (QTL). The majority of the Al tolerance genes identified so far belongs to two independent groups of gene families, i.e. aluminumactivated malate transporter (ALMT) and multidrug and toxic compound extrusion (MATE), both encoding transport proteins involved in Al-activated organic acid release, mainly citrate and malate. The variations in Al toxicity tolerance phenotypes are strongly correlated with the expressions of such genes in the root apical cells. Many Al tolerance QTLs have been mapped in the genomes of various crop species and were found to be colocated with the ALMT and MATE genes. The genetic maps of the Al tolerance genes and QTLs facilitate breeding programs for developing Al-tolerant cultivars through marker-assisted breeding methods. Al tolerance genes that have been isolated from genetically unrelated species can be used in genetic transformation studies of crop genotypes sexually incompatible to the gene source genotypes. The application of these molecular breeding methods expedites breeding programs to develop crop cultivars tolerance to Al toxicity and acid soils. Genomic technologies by using next-generation sequencing and high-throughput genotyping system accelerate Al toxicity tolerance gene and QTL discoveries of various crop species. The modern genomic technologies also facilitate more comprehensive PGR characterization and utilization to accelerate identification and isolation of the Al tolerance genes and QTLs to be used in a more comprehensive breeding program to support national food self sufficiency and food security programs.

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