scholarly journals Recombinant Lines Obtained from an Interspecific Cross between Lycopersicon Species Selected by Fruit Weight and Fruit Shelf Life

2006 ◽  
Vol 131 (5) ◽  
pp. 651-656 ◽  
Author(s):  
Gustavo R. Rodríguez ◽  
Guillermo R. Pratta ◽  
Roxana Zorzoli ◽  
Liliana A. Picardi
Keyword(s):  
Shelf Life ◽  
Selection Process ◽  
Interspecific Cross ◽  
Transgressive Segregation ◽  
Fruit Weight ◽  
Random Drift ◽  
Wild Tomato Species ◽  
Tomato Species ◽  
Null Response ◽  
Fruit Shelf Life

A cross was performed between Lycopersicon esculentum Mill. `Caimanta' and L. pimpinellifolium (Jusl.) Mill. accession LA722. Divergent-antagonistic selection for fruit weight and shelf life started in the F2 generation. Fruit shelf life showed transgressive segregation in this F2 generation. The selection process continued until the F6 generation, but we found that only fruit weight was responsive to selection. Seventeen recombinant lines (RILs) were analyzed for both traits. Nine of these RILs were obtained by the selection process. The other eight RILs were obtained by selfing without selection from the same F2 generation to assess random drift. Highly significant differences were found among these RILs for both fruit weight and shelf life. Random drift was as important as selection in producing different genotypes. Although fruit shelf life showed null response to selection in this interspecific cross, selfing and selecting has generated a new population of 17 recombinant genotypes for both fruit weight and shelf life. This experiment has demonstrated that wild tomato species offer breeders another possibility to enhance the genetic variability for fruit shelf life and fruit weight in tomato germplasm.

scholarly journals Exploration of Three Solanum Species for Improvement of Antioxidant Traits in Tomato

HortScience ◽  
2014 ◽  
Vol 49 (8) ◽  
pp. 1003-1009 ◽  
Author(s):  
Oğuz Top ◽  
Cantuğ Bar ◽  
Bilal Ökmen ◽  
Duygu Yüce Özer ◽  
Dane Rusçuklu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Phenolic Content ◽  
Agronomic Traits ◽  
Solanum Species ◽  
Transgressive Segregation ◽  
Fruit Weight ◽  
Water Soluble ◽  
Wild Tomato Species ◽  
Tomato Species

Wild tomato species have been widely used for improvement of tomato disease resistance but have not been extensively explored for health-related traits. In this work, three interspecific populations derived from backcrosses between cultivated tomato and Solanum pimpinellifolium (LA1589), S. habrochaites (LA1223), and S. peruvianum (LA2172) were analyzed for water-soluble antioxidant activity, phenolic content, vitamin C content, and basic agronomic traits including fruit weight, shape, and color. The wild species accessions significantly exceeded S. lycopersicum for all three antioxidant traits with only one exception: vitamin C content in S. habrochaites LA1223. Several populations and traits showed transgressive segregation indicating that the backcross populations contained individuals with allele combinations that allowed antioxidant activity/content to exceed that of both parents. The S. habrochaites LA1223 population provided the best starting material for improvement of water-soluble antioxidant activity and phenolics content with 20% and 15% of the population, respectively, significantly exceeding the parental values for these traits. Moreover, the S. habrochaites population contained individuals that had nearly 2-fold more water-soluble antioxidant activity and phenolic content than cultivated tomato. The S. peruvianum LA2172 population was best for improvement of vitamin C content with 3-fold variation for the trait and individuals, which had twice as much vitamin C as cultivated tomato.

scholarly journals Molecular markers detect stable genomic regions underlying tomato fruit shelf life and weight

2011 ◽  
Vol 11 (2) ◽  
pp. 157-164 ◽  
Author(s):  
Guillermo Raúl Pratta ◽  
Gustavo Rubén Rodriguez ◽  
Roxana Zorzoli ◽  
Estela Marta Valle ◽  
Liliana Amelia Picardi
Keyword(s):  
Shelf Life ◽  
Recombinant Inbred Lines ◽  
Tomato Fruit ◽  
Interspecific Cross ◽  
Fruit Weight ◽  
Wild Germplasm ◽  
Year Effect ◽  
Principal Effect ◽  
Fruit Shelf Life ◽  
Genomic Regions

Incorporating wild germplasm such as S. pimpinellifolium is an alternative strategy to prolong tomato fruit shelf life (SL) without reducing fruit quality. A set of recombinant inbred lines with discrepant values of SL and weight (FW) were derived by antagonistic-divergent selection from an interspecific cross. The general objective of this research was to evaluate Genotype x Year (GY) and Marker x Year (MY) interaction in these new genetic materials for both traits. Genotype and year principal effects and GY interaction were statistically significant for SL. Genotype and year principal effects were significant for FW but GY interaction was not. The marker principal effect was significant for SL and FW but both year principal effect and MY interaction were not significant. Though SL was highly influenced by year conditions, some genome regions appeared to maintain a stable effect across years of evaluation. Fruit weight, instead, was more independent of year effect.

scholarly journals Phenotypic diversity and chemical properties of pawpaw fruit quality in Ugandan germplasm

2020 ◽  
Vol 28 (4) ◽  
pp. 499-515
Author(s):  
E. Nuwamanya ◽  
O. Ampurire ◽  
Y. Mukasa ◽  
A. Katungisa ◽  
M. Kanaabi ◽  
...  
Keyword(s):  
Shelf Life ◽  
Phenotypic Diversity ◽  
Chemical Properties ◽  
Titratable Acidity ◽  
Fruit Production ◽  
Quality Parameters ◽  
Fruit Weight ◽  
Fruit Firmness ◽  
Fruit Pulp ◽  
Fruit Shelf Life

Pawpaw (Carica pawpaw L.) fruit production and utilisation have been on the increase in Uganda. However, challenges related to identification of phenotypes with inherent characteristics for improvement of fruit shelf life have limited identification of better varieties. The objective of this study was to evaluate the phenotypic diversity and shelf life determinants of Uganda’s pawpaw accessions and their variations based on selected quality parameters. Nineteen accessions were collected as seeds from different markets of Uganda, germinated and planted in Namulonge-Wakiso district, central Uganda. The accessions were significantly (P<0.05) variable in terms of fruit weight parameters, with dry matter ranging from 14 - 19% and fruit weight of up to 3.9 kg; while pulp weight was up to 3.2 kg per fruit. Pulp firmness parameters were also significantly variable (P<0.05) and highly correlated with shelf life of the fruit, which ranged from 7 - 13 days depending on the accession. Fruit pulp pH ranged from 4.4-5.6; while titratable acidity of the fruit ranged from 0.03-0.08%. The accessions were clustered in four main clusters depending on the fruit firmness properties, fruit shelf life, fruit weight, pH and titratable acidity as definitive parameters. These accessions were distinguished based on morphological parameters, and henceforth defined for nutritional and economic uses. Accessions with high fruit firmness (>5 kg F) such as 16/20’ 16/16, 16/17 and 18/1; and external pulp thickness (>2 cm) such as 16/16, 16/17 and 18/1 had improved fruit shelf life and are recommended for marketability and processing.

Guava (Psidium guajava L.) Fruit Coating with Gum – Arabic for Quality and Fruit Fly Control

1970 ◽  
pp. 01-04
Author(s):  
Esameldin B. M. Kabbashi, Ghada H. Abdelrahman and Nawal A. Abdlerahman
Keyword(s):  
Shelf Life ◽  
Gum Arabic ◽  
Fruit Fly ◽  
Psidium Guajava ◽  
Postharvest Quality ◽  
Guava Fruit ◽  
Fruit Shelf Life ◽  
Fly Control ◽  
And Control ◽  
Subtropical Fruit

Guava (Psidium guajava L.) is a lovely tropical and subtropical fruit that originates in Mexico, Central America, and then taken to other distant and near parts around the world. In Sudan this popular fruit is produced in orchards and household and is so profitable but yet attacked by a lot of fruit fly species of the Genera Ceratitis and Bactrocera and the result is a loss of more than 70%. This research aimed at evaluating the effect of Gum Arabic coating (GAC) in extending the shelf life of guava fruit and disinfesting it from these notorious pests. Guava fruits from Kadaro orchards, Khartoum North, were tested using seven concentrations of Gum Arabic solutions. The results reflect that 1: 4 (25%) and 1: 8 (12.5%) (GA: water) concentrations attained 56 and 40% disinfestation, respectively whereas the other lower concentrations effected corresponding results in a range from 20 – 08%. The reduction in maggots per test fruit reached upto 188% as compared to the control.  The highest concentrations (1: 4 & 1: 8) effected a sustainability of 52% in fruit firmness (FF) with an average of medium (3) FF compared to soft FF (4) in the control. The corresponding results in other lower concentrations (1: 16; 1: 32; 1: 64; 1: 72 & 1: 96) were 36, 24, 24, 20 and 16%, respectively. In addition to an average FF of 4 (soft) for all these concentrations and 5 (very soft) for all the corresponding controls. Nevertheless, the sustainability of fruit color (FC) effected by the test concentrations was 52, 44, 24, 22, 24, 20, and 24%, respectively. Regarding these results, the two highest test concentrations effected a sizeable disinfestation and control of fruit flies and a good extension of shelf life of guava in Khartoum State. These findings support using this treatment as an effective IPM tool to extend guava fruit shelf life and upgrading its postharvest quality.

ANTIBACTERIAL, ANTIFUNGAL ACTIVITY OF COMPOSITE EDIBLE COATING INCORPORATED WITH CITRUS LIMON L. PEEL EXTRACT AND IT’S EFFECT ON FUJI APPLES.

2020 ◽  
Author(s):  
Renu Jaisinghani ◽  
Vishnu Vasant Dayare
Keyword(s):  
Shelf Life ◽  
Antimicrobial Properties ◽  
Edible Coating ◽  
Dilution Method ◽  
Citrus Limon ◽  
Edible Coatings ◽  
Lemon Peel ◽  
Fruit Shelf Life ◽  
Broth Dilution ◽  
Peel Extract

Edible film and coatings are been looked upon for preservation of fruits, vegetables and bakery products. Extended shelf life with preservation of natural properties of food is always been a challenge; by incorporation of bio-actives in edible coatings, the shelf life can be increased as they are known for their antioxidant and antimicrobial properties. With this view, present study was undertaken, where edible coatings were prepared from starch, gelatin and glycerol and incorporated with Lemon peel extracts and coated on apples for increased shelf life. Antimicrobial activity of Lemon peel extracts was studied on eight organisms by broth dilution method and were found to be effective at concentration 3mg/mL-9mg/mL for bacteria and 50mg/mL- 90mg/mL for fungi. Fuji apples coated with starch-gelatin based edible formulation containing lemon peel extracts were studied for the effect of coating on fruit shelf life during storage for 28 days. Incorporation of lemon peel extract into edible coating improved shelf life with reducing rate of browning of apples.

scholarly journals Engineering Melon Plants with Improved Fruit Shelf Life Using the TILLING Approach

PLoS ONE ◽  
2010 ◽  
Vol 5 (12) ◽  
pp. e15776 ◽  
Author(s):  
Fatima Dahmani-Mardas ◽  
Christelle Troadec ◽  
Adnane Boualem ◽  
Sylvie Lévêque ◽  
Abdullah A. Alsadon ◽  
...  
Keyword(s):  
Shelf Life ◽  
Fruit Shelf Life

scholarly journals Synthetic acylsugars and their effects on the control of arthropod pests

2017 ◽  
Vol 41 (2) ◽  
pp. 201-208
Author(s):  
Anni Cristini Silvestri Gomes ◽  
Maria das Graças Cardoso ◽  
Juliano Vilela Resende ◽  
Sérgio Scherrer Thomasi ◽  
Luana Isac Soares ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Biological Effect ◽  
Sodium Acetate ◽  
Spectroscopic Techniques ◽  
Agricultural Pests ◽  
Wild Tomato Species ◽  
Tomato Species ◽  
Biological Tests ◽  
Arthropod Pests ◽  
Low Rate

ABSTRACT One of the main problems facing agriculture is the loss of production as a result of the attack of agricultural pests. Alternative ways to work around this problem are being sought. There are substances called acylsugars that are naturally produced by the wild tomato species S. pennellii and affect arthropod pests. The objectives of this work were to synthesize two acylsugars and assess the biological effect of these on the arthropod pests Bemissia tabaci and Tetranycus urticae. The syntheses were performed via the reactions of glucose and sucrose (saccharose) with acetic anhydride using sodium acetate as the catalyst. The products of these reactions were sucrose octa-acetate and glucose penta-acetate, the structures of which were confirmed by spectroscopic techniques. In a resistance test against the mite, a linear correlation between the concentration of the synthesized substances, and the dislocation of the mite was obtained. A delay in the hatching of the arthropod eggs was observed, causing a mortality rate of approximately 95% in the 1st instar larvae of mites that was confirmed in adults. In the biological tests with Bemisia tabaci, there was a low rate of hatching and emergence, and the effect on the nymphs was the deformation of the emergent adults.

scholarly journals Chitosan-based Edible Coating Prolongs Musa troglodytarum L. (‘Pisang Tongkat Langit’) Fruit Shelf-life and Changes the ACS1 and ACO1 Gene Expression Profile

2020 ◽  
Vol 43 (4) ◽  
Author(s):  
Cindy Novianti ◽  
Fenny Martha Dwivany
Keyword(s):  
Gene Expression ◽  
Shelf Life ◽  
Expression Pattern ◽  
Expression Analysis ◽  
Fruit Ripening ◽  
Gene Expression Analysis ◽  
Gene Expression Pattern ◽  
Edible Coating ◽  
Chitosan Coating ◽  
Fruit Shelf Life

Musa troglodytarum L. (‘Pisang Tongkat Langit’), a banana cultivar which originated from Eastern Indonesia, has an economic potential due to the high β-carotene content on its pulp. Being a climacteric fruit, M. troglodytarum has a short shelf-life that can reduce fruit quality. In this study, the effect of 1.25% (w/v) chitosan coating on M. troglodytarum fruit shelf-life and ACS1 and ACO1 gene expression analysis using quantitative PCR were evaluated. Results showed that the application of chitosan coating delayed the fruit ripening process for two days by delaying several fruit physical and chemical changes. ACS1 and ACO1 gene expression analysis showed a different expression pattern, the expression level was lower on chitosan-coated fruits on the first day compared to control. In conclusion, chitosan-based edible coating delayed M. troglodytarum fruit ripening and changed the ACS1 and ACO1 gene expression pattern, compared with the chitosan coating effect on Cavendish banana which also prolonged fruit ripening and suppressed ACS1 and ACO1 expression in a previous research.

scholarly journals PEMANFAATAN ASAP CAIR ALANG-ALANG (Imperata cylindrica) SEBAGAI PENGAWET TERHADAP KARAKTERISTIK BUAH PISANG MAKAU (Musa spp.)

AGROINTEK ◽  
2018 ◽  
Vol 12 (1) ◽  
pp. 39
Author(s):  
Dian Fitriarni ◽  
Rafika Ayuni
Keyword(s):  
Vitamin C ◽  
Shelf Life ◽  
Raw Materials ◽  
Sugar Content ◽  
Fruit Weight ◽  
Imperata Cylindrica ◽  
Alternative Methods ◽  
Musa Spp ◽  
Percentage Decrease ◽  
Liquid Smoke

<em>Research on the utilization of liquid smoke especially for preservation of fruit has been done and become one of alternative methods that can be used to maintain fruit shelf life. Liquid smoke raw materials can come from a variety of sources one of which comes from the Alang-alang (Imperata cylindrica). This study aims to determine the ability of liquid smoke made from  alang-alang as a preservative of bananas and to know the effect on the characteristics of bananas. The sample used in this research is a local banana known as Pisang Makau (Musa spp). This research uses experimental method with 7 days of storage time. Initial stages of the research are cleaning the fruit, dyeing, drying, and storage at room temperature. This research uses liquid smoke from alang-alang by using 2 various concentrations 50% and 100%. The next stage is the analysis of the characteristics of bananas such as color, fruit texture, fruit weight, sugar content, and vitamin C levels. Based on the analysis results obtained by Pisang Makau with the treatment of liquid has a longer shelf life than the control. Based on the results of the analysis of the shelf life of fruit treated with liquid smoke decreased levels of sugar and vitamin C levels. Percentage decrease levels of sugar and vitamin C between the fruit with the treatment and control not much different. This shows that liquid smoke from alang-alang has the ability to retain the shelf life of bananas without affecting sugar and vitamin C levels in bananas in this case for Pisang Makau. Levels of sugar and vitamin C in bananas decreased during the process of storage and bunding of fruit</em>

scholarly journals Transcriptome resequencing analysis of the responses of Ty-5-Mediated resistance to TYLCV via in resistant vs. susceptible tomato cultivars

2018 ◽  
Author(s):  
Qingqi Chen ◽  
Xiangyang Xu ◽  
Jingbin Jiang ◽  
Jingfu Li
Keyword(s):  
Molecular Mechanisms ◽  
Carbon Fixation ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Glutathione Metabolism ◽  
Functional Classification ◽  
Wild Tomato Species ◽  
Tomato Species ◽  
Tomato Cultivars ◽  
Susceptible Tomato

Tomato yellow leaf curl virus (TYLCV) is one of the most devastating viruses of cultivated tomato in both tropical and subtropical regions. Five major genes (Ty-1, Ty-2, Ty-3, Ty-4 and Ty-5) from wild tomato species have been associated with resistance to TYLCV. Researchers have recently attempted to determine the functions of these resistance genes, but molecular mechanisms underlying the observed resistance remain unclear. Here, resistant (cv. CLN3212A-23, carrying Ty-5) and susceptible (cv. Moneymaker) plants were either left untreated (R and S, respectively) or artificially inoculated with TYLCV via Agrobacterium-mediated transformation (RT and ST, respectively). The transcriptomes of the plants in the four groups were then analyzed by RNA-Seq, and the results identified 8,639 differentially expressed genes (DEGs) between the R and RT groups, 2,818 DEGs between the RT and ST groups, 8,899 DEGs between the S and ST groups, and 707 DEGs between the R and S groups. The gene expression profiles in both the resistant and susceptible tomato cultivars appeared to undergo notable changes after viral inoculation, and functional classification revealed that most DEGs were associated with 18 GO terms. Moreover, the functional classification of the response of Ty-5-carrying tomato plants to TYLCV infection identified the importance of the GO term “response to stimulus” in the BP category, which is related to disease resistance. In addition, 28 genes were significantly enriched in the “Plant hormone signal transduction”, “Carbon metabolism”, “ Carbon fixation in photosynthetic organisms ” and “ Glutathione metabolism ” pathways. The differential expression levels of 12 select genes were confirmed by quantitative real-time PCR. The present study indicates that the Ty-5 gene activates the expression of multiple genes involved in the resistance process and will aid a more in-depth understanding of the effects of the Ty-5 gene on resistance based on its molecular mechanism with the aim of improving TYLCV disease management in tomato.

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