In vitro biocompatibility of calcined mesoporous silica particles and fetal blood cells

To develop a kind of gastrointestinal timed-release preparation for Metoprolol Tartrate, nanostructured silica particles were chose for the purpose. Briefly, MCM-41 type mesoporous silica nanospheres with a size of 100-200 nm were synthesized through the reaction of tetraethyl orthosilicate (TEOS) in the water medium at 353 K, with introducing some cetyltrimethyl ammonium bromide (CTAB) as porogens. Various analytical methods, including FT-IR, XRD, TEM, N2 physisorption and thermal analysis, were applied to characterize the final products. Metoprolol Tartrate was then loaded into the mesoporous silica nanospheres by soaking. Results of the release of the drug in simulated gastric juice indicated that the drug can release up to 24 h and its maximum released amount was 4.5%. In the simulated intestinal juice the maximum cumulative released amount of metorprolol was 10.8%.In vitro release behavior revealed that the mesoporous silica were appropriate used as drug delivery system.

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Surfactant-Triggered Molecular Gate Tested on Different Mesoporous Silica Supports for Gastrointestinal Controlled Delivery

Nanomaterials ◽

10.3390/nano10071290 ◽

2020 ◽

Vol 10 (7) ◽

pp. 1290 ◽

Cited By ~ 1

Author(s):

Elisa Poyatos-Racionero ◽

Isabel González-Álvarez ◽

Marta González-Álvarez ◽

Ramón Martínez-Máñez ◽

M. Dolores Marcos ◽

...

Keyword(s):

Mesoporous Silica ◽

In Vitro Digestion ◽

Wistar Rat ◽

Porous Matrix ◽

Silica Particles ◽

In Vivo Model ◽

Silica Supports ◽

Administration Methods

In recent decades, the versatility of mesoporous silica particles and their relevance to develop controlled release systems have been demonstrated. Within them, gated materials able to modulate payload delivery represent great advantages. However, the role played by the porous matrix in this kind of systems is scarce. In this work, different mesoporous silica materials (MCM-41, MCM-48, SBA-15 and UVM-7) are functionalized with oleic acid as a molecular gate. All systems are fully characterized and their ability to confine the entrapped cargo and release it in the presence of bile salts is validated with release assays and in vitro digestion experiments. The cargo release profile of each synthesized support is studied, paying attention to the inorganic scaffold. Obtained release profiles fit to Korsmeyer–Peppas model, which explains the differences among the studied supports. Based on the results, UVM-7 material was the most appropriate system for duodenal delivery and was tested in an in vivo model of the Wistar rat. Payload confinement and its complete release after gastric emptying is achieved, establishing the possible use of mesoporous silica particles as protection and direct release agents into the duodenum and, hence, demonstrating that these systems could serve as an alternative to the administration methods employed until now.

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Stability of different mesoporous silica particles during an in vitro digestion

Microporous and Mesoporous Materials ◽

10.1016/j.micromeso.2016.05.004 ◽

2016 ◽

Vol 230 ◽

pp. 196-207 ◽

Cited By ~ 13

Author(s):

Édgar Pérez-Esteve ◽

María Ruiz-Rico ◽

Cristina de la Torre ◽

Empar Llorca ◽

Félix Sancenón ◽

...

Keyword(s):

Mesoporous Silica ◽

In Vitro Digestion ◽

Silica Particles

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Development of a gliclazide ionic liquid and its mesoporous silica particles: an effective formulation strategy to improve oral absorption properties

RSC Advances ◽

10.1039/d1ra07499g ◽

2022 ◽

Vol 12 (2) ◽

pp. 1062-1076

Author(s):

Bijian Zhou ◽

Dan Teng ◽

Jinghui Li ◽

Yanhong Zhang ◽

Minghui Qi ◽

...

Keyword(s):

Ionic Liquid ◽

Mesoporous Silica ◽

Oral Absorption ◽

Silica Particles ◽

Dissolution Behavior ◽

Absorption Properties ◽

Enhanced Solubility

An ionic liquid (IL) form of gliclazide with enhanced solubility characteristics was successfully synthesized. This IL could be loaded into mesoporous silica carrier and exhibited improved dissolution behavior in vitro.

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In Vitro Effects of Artesunate on Rat Embryonic and Fetal Blood Cells

Microscopy and Microanalysis ◽

10.1017/s1431927607076830 ◽

2007 ◽

Vol 13 (S02) ◽

Cited By ~ 2

Author(s):

BE Maleeff ◽

AC James ◽

SB Laffan ◽

RL Clark ◽

TE K White

Keyword(s):

Blood Cells ◽

Fetal Blood ◽

In Vitro Effects

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Real-Time Tracking of Highly Luminescent Mesoporous Silica Particles Modified with Europium β-Diketone Chelates in Living Cells

Nanomaterials ◽

10.3390/nano11020343 ◽

2021 ◽

Vol 11 (2) ◽

pp. 343

Author(s):

Jong-Seok Kim ◽

Sung Lee ◽

Hansol Doh ◽

Myeong Kim ◽

Do Kim

Keyword(s):

Mesoporous Silica ◽

Silica Particles ◽

Mouse Bone Marrow ◽

High Concentration ◽

Europium Complexes ◽

Imaging Probes ◽

In Vitro Diagnostic ◽

Real Time Tracking

Highly luminescent europium complexes modified mesoporous silica particles (MSP) were synthesized as an imaging probes for both in-vitro diagnostic and in-vivo cellular tracking agents. Europium β-diketone chelates (4,4,4-trifluoro-l-(2-thienyl)-l,3-butanedione) trioctylphosphine europium (III) (Eu(TTA)3(P(Oct)3)3) were incorporated inside the nanocavities that existed in hierarchical MSP (Eu@MSP). The MSP and Eu@MSP on mouse bone marrow-derived macrophages (BMDMs) did not show any toxic effect. The MSP and Eu@MSP in the BMDMs were found at cytoplasm without any degradation and immunogenicity. However, both pro- and anti-inflammatory cytokines of macrophages were significantly increased when lipopolysaccharide and a high concentration (100 μg/mL) of MSP and Eu@MSP were treated simultaneously.

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Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100162132 ◽

1990 ◽

Vol 48 (3) ◽

pp. 914-915

Author(s):

D.J.P. Ferguson ◽

A.R. Berendt ◽

J. Tansey ◽

K. Marsh ◽

C.I. Newbold

Keyword(s):

Plasmodium Falciparum ◽

Red Blood Cells ◽

Blood Cells ◽

Umbilical Vein ◽

Cell Types ◽

Amelanotic Melanoma ◽

Cytoplasmic Process ◽

Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

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Mechanism of the Antiplatelet Action of Dipyridamole in Whole Blood: Modulation of Adenosine Concentration and Activity

Thrombosis and Haemostasis ◽

10.1055/s-0038-1661437 ◽

1986 ◽

Vol 55 (01) ◽

pp. 012-018 ◽

Cited By ~ 86

Author(s):

Paolo Gresele ◽

Jef Arnout ◽

Hans Deckmyn ◽

Jos Vermylen

Keyword(s):

Platelet Aggregation ◽

Adenosine Receptor ◽

Whole Blood ◽

Blood Cells ◽

Inhibitory Action ◽

Phosphodiesterase Inhibitor ◽

Inhibitory Effect ◽

Adenosine Concentration ◽

Pharmacological Studies

SummaryDipyridamole inhibits platelet aggregation in whole blood at lower concentrations than in plasma. The blood cells responsible for increased effectiveness in blood are the erythrocytes. Using the impedance aggregometer we have carried out a series of pharmacological studies in vitro to elucidate the mechanism of action of dipyridamole in whole blood. Adenosine deaminase, an enzyme breaking down adenosine, reverses the inhibitory action of dipyridamole. Two different adenosine receptor antagonists, 5’-deoxy-5’-methylthioadenosine and theophylline, also partially neutralize the activity of dipyridamole in blood. Enprofylline, a phosphodiesterase inhibitor with almost no adenosine receptor antagonistic properties, potentiates the inhibition of platelet aggregation by dipyridamole. An inhibitory effect similar to that of dipyridamole can be obtained combining a pure adenosine uptake inhibitor (RE 102 BS) with a pure phosphodiesterase inhibitor (MX-MB 82 or enprofylline). Mixing the blood during preincubation with dipyridamole increases the degree of inhibition. Lowering the haematocrit slightly reduces the effectiveness.Although we did not carry out direct measurements of adenosine levels, the results of our pharmacological studies clearly show that dipyridamole inhibits platelet aggregation in whole blood by blocking the reuptake of adenosine formed from precursors released by red blood cells following microtrauma. Its slight phosphodiesterase inhibitory action potentiates the effects of adenosine on platelets.

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