EVALUATION OF TWO METHODS OF GERMPLASM COLLECTION IN MEXICO SOUTHEAST

The implementation of good farming practices in seed collecting is fundamental key to guarantee the physiological and genetic quality of forest seeds. The objective of this work was to evaluate the implementation of two methods to collect seedsfrom Cedrelaodorata L.toguarantee seeds of high physiologicalquality. Seeds from 40 superior phenotypically trees of Cedrelaodorata L. in Yucatan Mexico peninsula region were collected from March to May 2019.The collect methods applied in this research werethe traditionalcollectand the climb trees. The physiological quality of seeds wasevaluated by germination and tetrazolium test. The laboratory analysis confirmed 88% of seeds pure with 94% of germination in climbing trees method, in contrast in the traditional method showed 66% of seeds pure with 72% of germination. The results by conventional germination it was not showed significative statistical differences. These results are part of novel contributions in collectmethodology using tools to climb on trees which to be complement of superior phenotypically trees selection, contribute comprehensively to obtain physiological and genetic quality forest seeds.

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TESTE DE TETRAZÓLIO EM SEMENTES DE Copaifera langsdorffii E Schizolobium parahyba

FLORESTA ◽

10.5380/rf.v41i4.25352 ◽

2011 ◽

Vol 41 (4) ◽

Cited By ~ 3

Author(s):

Cristiane Alves Fogaça ◽

Nádia Graciele Krohn ◽

Márcio De Aquino Souza ◽

Rinaldo César de Paula

Keyword(s):

Seed Coat ◽

Germination Test ◽

Schizolobium Parahyba ◽

Copaifera Langsdorffii ◽

Physiological Quality ◽

Forest Seeds ◽

Tetrazolium Test

Este trabalho objetivou padronizar o teste de tetrazólio para avaliação da viabilidade de sementes de copaíba e guapuruvu. Avaliou as seguintes metodologias: sementes escarificadas mecanicamente e embebidas por 24 e 48 horas, com posterior retirada do tegumento e imersas em solução de tetrazólio a 0,075, 0,10 e 0,20% por 2, 3 e 4 horas, a 35 ºC, no escuro. As metodologias avaliadas que foram eficientes na obtenção de coloração satisfatória, permitindo a diferenciação dos tecidos, e na avaliação da qualidade fisiológica das sementes, quando comparadas com o teste padrão de germinação, foram: para a espécie copaíba, sementes escarificadas e embebidas por 24 horas, a 35 ºC, com posterior retirada do tegumento, submetidas a solução de tetrazólio a 0,20% por 4 horas, a 35 ºC, no escuro; e para a espécie guapuruvu, sementes escarificadas e embebidas por 48 horas, a 35 ºC, com posterior retirada do tegumento, submetidas a solução de tetrazólio a 0,10% por 4 horas, a 35 ºC, no escuro.Palavras-chave: Sementes florestais; teste rápido; copaíba; guapuruvu. AbstractTetrazolium test in Copaifera langsdorffii and Schizolobium parahyba seeds. This research aimed to standardize the tetrazolium test for evaluation of viability of Copaifera langsdorffii Desf. and Schizolobium parahyba Vell. Blake seeds. It evaluated the following methodologies: seeds scarificated mechanically and soaked up by 24 and 48 hours, with posterior seed coat removal and immersed in tetrazolium solution at 0.075; 0.10 and 0.20% for 2, 3 and 4 hours, 35 ºC, the dark one. The evaluated methodologies that had been efficient in the attainment of satisfactory coloration, allowing the differentiation of tissues, and in the evaluation of the physiological quality of the seeds when compared with the germination test, had been: for the species Copaifera langsdorffii, seeds scarificated and soaked up by 24 hours, 35 ºC, with posterior seed coat removal, submitted to the tetrazolium solution 0.20% for 4 hours, 35 ºC, in the dark one, and for the species guapuruvu, seeds scarificated and soaked up by 48 hours, 35 ºC, with posterior seed coat removal, submitted to the tetrazolium solution 0.10% for 4 hours, 35 ºC, in the dark one.Keywords: Forest seeds; fast test; Copaifera langsdorffii; Schizolobium parahyba.

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PRODUCTION AND PHYSIOLOGICAL QUALITY OF SOYBEAN SEEDS IN ORTHIC QUARTZARENIC NEOSOIL OF THE CERRADO REGION

Revista Caatinga ◽

10.1590/1983-21252015v28n414rc ◽

2015 ◽

Vol 28 (4) ◽

pp. 127-134

Author(s):

CHARLINE ZARATIN ALVES ◽

GUSTAVO MARTINS ZAQUEU ◽

ERIC FABIANO SERAGUZI ◽

AGUINALDO JOSÉ FREITAS LEAL ◽

JOSUÉ BISPO DA SILVA

Keyword(s):

Electrical Conductivity ◽

Aerial Part ◽

Water Distribution ◽

Accelerated Aging ◽

Dry Mass ◽

Soybean Seeds ◽

Scheme Design ◽

Physiological Quality ◽

Tetrazolium Test

ABSTRACT: The aim of this study was to evaluate the effect of the sowing periods in production, productivity component and physiological quality of seeds of three soybean cultivars (TMG133RR, P98Y70RR and NS7670RR) in Orthic Quartzarenic Neosoil in the cerrado region. The design used was a randomized block a factorial scheme design with four repetitions, and each plot with useful space consisted of three rows of four meters length, spaced at 0.45 meters. Field evaluations were the final stand, the height of the plants, height of the first pod insertion, the mass of 100 seeds and productivity. Already in the lab seeds were evaluated for germination and vigor (first germination count, emergency, emergence speed index, length and dry mass of the aerial part of the plant and roots, electrical conductivity, accelerated aging and tetrazolium test). It concludes that it is possible to use Orthic Quartzarenic Neosoils, located in the Cerrado region at altitudes higher than 600 m, in years and places with good water distribution in the spring-summer seasons, for grain and soybeans production, but as a first goal, the crop should be sown in the first 20 days of November otherwise it should take place in early December.

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Viability of Carica papaya L. Seeds by the Tetrazolium Test

Journal of Agricultural Science ◽

10.5539/jas.v10n2p335 ◽

2018 ◽

Vol 10 (2) ◽

pp. 335 ◽

Cited By ~ 2

Author(s):

Sara M. C. Carvalho ◽

Salvador B. Torres ◽

Erivanessa C. Sousa ◽

Danielle M. M. Sousa ◽

Kleane T. O. Pereira ◽

...

Keyword(s):

Decision Making ◽

Carica Papaya ◽

Seed Viability ◽

Germination Test ◽

Long Period ◽

Physiological Quality ◽

Rapid Tests ◽

Papaya Seeds ◽

Tetrazolium Test

Germination test for papaya seeds demands thirty days to obtain the results, which is a long period for decision-making. Thus, rapid tests become essential tools to evaluate seed viability, and the tetrazolium test is an alternative for this purpose. Hence, this study aimed to establish the adequate procedure to apply this test, in order to reduce the time of evaluation of papaya seeds. For that, four tetrazolium solution concentrations (0.05, 0.1, 0.5 and 1%) and three staining times (3, 6 and 9 hours) were tested at temperatures of 35 and 40 ºC. The experimental design was completely randomized in 4 × 3 + 1 factorial scheme (concentrations × staining times + one control = germination test). It was found that the tetrazolium test allows to evaluate the physiological quality of papaya seeds, thus reducing the time for decision-making. For the test, we recommend using the concentrations of 0.1% for 9 hours or 1% for 6 hours of staining, at temperature of 40 ºC.

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Methodology of the tetrazolium test for assessing the viability of seeds of Eugenia brasiliensis Lam., Eugenia uniflora L. and Eugenia pyriformis Cambess

Journal of Seed Science ◽

10.1590/2317-1545v36n41029 ◽

2014 ◽

Vol 36 (4) ◽

pp. 427-434 ◽

Cited By ~ 6

Author(s):

Edmir Vicente Lamarca ◽

Claudio José Barbedo

Keyword(s):

Electrical Conductivity ◽

Seed Viability ◽

Important Method ◽

Eugenia Uniflora ◽

Fruit Species ◽

Incubation Periods ◽

Physiological Quality ◽

Different Levels ◽

Tetrazolium Test

Eugenia brasiliensis Lam. ("grumixameira"), Eugenia uniflora L. ("pitangueira") and Eugenia pyriformis Cambess. ("uvaieira") are forest and fruit species of pharmacological and gastronomic potential and have desiccation-sensitive seeds. The development of quick viability tests for the assessment of physiological quality of these seeds is needed. The tetrazolium test is an important method providing fast assessment of the seed physiological quality. Thus, this work aimed to develop a method for the tetrazolium test for determining viability of "grumixameira," "pitangueira" and "uvaieira" seeds. Initially the seeds of these species were soaked in water for 3 h at 25 ºC. Three concentrations of tetrazolium solutions were analyzed (0.100 %, 0.125 % and 0.250 %) for seed staining using three incubation periods (2, 3 and 6 h) at 35 ºC in the dark. After staining, seed viability was determined. Then, the seeds were subjected to different levels of controlled drying and were analyzed for their physiological quality by germination and electrical conductivity tests. The tetrazolium test is a suitable tool for determining viability after staining the seeds at 35 ºC using a 0.250 % concentration solution for 3 hours for "grumixameira" seeds, 0.125 % for 3 hours for "pitangueira" seeds and 0.100 % for 2 hours for "uvaieira" seeds.

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Seed quality analysis of Senna siamea Lam. using image analysis techniques

Journal of Seed Science ◽

10.1590/2317-1545v42241633 ◽

2020 ◽

Vol 42 ◽

Author(s):

Jackson Araújo da Silva ◽

André Dantas de Medeiros ◽

Márcio Dias Pereira ◽

Amanda Karoliny Fernandes Ramos ◽

Laércio Junio da Silva

Keyword(s):

Image Analysis ◽

Seed Quality ◽

Quality Analysis ◽

Radiographic Images ◽

Senna Siamea ◽

Analysis Techniques ◽

Physiological Quality ◽

Image Analysis Techniques ◽

Forest Seeds

Abstract: The inclusion of image analysis techniques for the accurate and rapid assessment of the quality of forest seeds is highly desirable. The use of digital radiographic images of seeds and the analysis of seedling images, still little used in determining the quality of forest seeds, are among the available imaging techniques. This study aimed to assess the feasibility of using the analysis of images of seeds and seedlings in the assessment of the physical and physiological quality of Senna siamea seeds. Radiographic images of seeds were obtained from five seed lots, allowing generating morphological and tissue integrity descriptors. These seeds were then subjected to germination and seedling growth tests, which allowed obtaining variables related to physiological quality. The generated seedlings were scanned and analyzed using the software ImageJ. The data were analyzed using analysis of variance, correlation, and principal component analysis. The results showed differences between seed lots in terms of physiological quality and physical integrity of internal tissues. Significant correlations were observed between the variables obtained with the radiographic analysis and seed physiological characterization tests. The use of techniques to analyze seed radiographs and seedling images allows access to information on the physical and physiological integrity of S. siamea seeds.

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Qualidade Física e Fisiológica de Sementes Comercializadas de Capim-Colonião em Campo Grande - MS

Ensaios e Ciência C Biológicas Agrárias e da Saúde ◽

10.17921/1415-6938.2020v24n1p07-11 ◽

2020 ◽

Vol 24 (1) ◽

pp. 07-11

Author(s):

Silvia Rahe Pereira ◽

Adriana Paula D’Agostini Contreiras-Rodrigues ◽

Daiane Rodrigues Oliveira ◽

Valdemir Antônio Laura ◽

Daniela Aparecida Dalla-Costa

Keyword(s):

Cultural Value ◽

Germination Test ◽

Tropical Regions ◽

Genetic Potential ◽

Randomized Design ◽

Physiological Quality ◽

Completely Randomized Design ◽

Maximum Expression ◽

Tetrazolium Test

O Centro-Oeste brasileiro representa a região brasileira com maior área e produção de sementes de Megathyrsus maximus, uma gramínea muito utilizada para a formação de pastagens em regiões tropicais do Brasil e outros países da América. Objetivou-se no presente estudo diagnosticar a qualidade física e fisiológica de sementes de três cultivares da espécie M. maximus (Mombaça, BRS Tamani e BRS Zuri) comercializadas por diferentes empresas de Campo Grande (MS). Os experimentos foram conduzidos utilizando-se sementes da safra 2017-2018 em um delineamento inteiramente casualizado, em esquema fatorial 3 x 6, avaliando-se três cultivares e seis procedências. As variáveis analisadas foram: pureza física, peso de mil sementes, primeira contagem do teste de germinação, germinação, valor cultural e teste de tetrazólio. Os resultados encontrados demonstram que, com exceção de um lote da cultivar Mombaça, os demais lotes das três cultivares da espécie Megathyrsus maximus (Mombaça, BRS Tamani e BRS Zuri), de todas as procedências avaliadas, apresentaram qualidade física e fisiológica compatível com a comercialização em função da legislação vigente. No entanto, é possível encontrar lotes com qualidade distinta no mercado de Campo Grande. Assim, a escolha adequada da procedência (empresa) vai garantir a expressão máxima do potencial genético, subsidiada por processos adequados de produção e pós-colheita. Palavras-chave: Megathyrsus Mombaça. Gramíneas Tropicais. Teste de Germinação. Teste de Tetrazólio. Valor Cultural. Abstract Brazilian's Midwest represents the region with the largest area and production of Megathyrsus maximus seeds, a grass widely used for the pastures formation in tropical regions of Brazil and other countries in America.The objective of this study was to diagnose the physical and physiological quality of seeds of three cultivars of the species Megathyrsus maximus (Mombaça, BRS Tamani and BRS Zuri) commercialized by different companies of Campo Grande (MS). The experiments were conducted using seeds of the 2017-2018 harvest, in a completely randomized design, using a factorial scheme 3 x 6, with three species and six sources, totaling 18 treatments. The variables analyzed were: physical purity, weight of one thousand seeds, first count of germination test, germination, cultural value and tetrazolium. The results showed that with the exception of one lot of cultivar Mombaça, the other lots of the three cultivars of species Megathyrsus maximus (Mombasa, BRS Tamani and BRS Zuri), of all provenances, have compatible physical and physiological quality with marketing in accordance with current legislation. However, it is possible to find lots of different quality in the Campo Grande market. Thus, the appropriate choice of source (company) will guarantee the maximum expression of genetic potential, subsidized by adequate production and post-harvest processes. Keywords: Megathyrsus mombaça. Tropical Grasses. Germination Test. Tetrazolium Test. Cultural Value.

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Processing and physical and physiological quality of the native forest seeds of Vernonanthura discolor

Acta Scientiarum Agronomy ◽

10.4025/actasciagron.v41i1.39574 ◽

2018 ◽

Vol 41 (1) ◽

pp. 39574

Author(s):

Camila Ribeiro de Souza Grzybowski ◽

Rosemeire Carvalho da Silva ◽

Elisa Serra Negra Vieira ◽

Maristela Panobianco

Keyword(s):

Native Forest ◽

Physiological Quality ◽

Forest Seeds

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Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds

Journal of Seed Science ◽

10.1590/2317-1545v41n1187777 ◽

2019 ◽

Vol 41 (1) ◽

pp. 7-12 ◽

Cited By ~ 1

Author(s):

Humberto Alencar Paraíso ◽

Delacyr da Silva Brandão Junior ◽

Ramon Ivo Soares Avelar ◽

Candido Alves da Costa ◽

Luan Souza de Paula Gomes ◽

...

Keyword(s):

Seed Quality ◽

Class Ii ◽

Class I ◽

Class Iii ◽

Test Methodology ◽

Physiological Quality ◽

Tetrazolium Test ◽

Class Iv

Abstract: Quick tests are essential tools to evaluate seed quality. The objective of this study was to adapt the methodology of tetrazolium test, by identifying the most suitable pre-conditioning parameters of imbibition temperature and duration, and tetrazolium solution concentrations, in order to assess the physiological quality of chickpea seeds. Also, this work proposed the separation of lots in classes, according to the viability and vigor of the seeds. Three lots of chickpeas (lot 1 - BRS Aleppo C1, lot 2 - BRS Aleppo basic, and lot 3 - Cicero) were evaluated. They were analyzed according to three combinations of imbibition temperature and duration (41 °C for 4 hours, 41 ºC for 6 hours, and 30 ºC for 18 hours) and two tetrazolium concentrations (0.1% and 0.5%). The imbibition at 41 °C for 4 or 6 hours, and at 30 ºC for 18 hours allowed clear visualization of injuries in the seeds after immersion in 0.1% tetrazolium solution. The intense coloration formed by the 0.5% solution of this salt prevented the differentiation of the types of damage. In addition, it was possible to separate the seeds into four classes: class I (viable and vigorous, without damages), class II (viable and vigorous, with superficial injuries), class III (viable and non-vigorous), and class IV (non-viable).

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Optimization of the tetrazolium test in three species of orchids of the Andean forest

Australian Journal of Crop Science ◽

10.21475/ajcs.20.14.05.p2276 ◽

2020 ◽

pp. 822-830

Author(s):

Seir Antonio Salazar Mercado ◽

Jesús David Quintero Caleño ◽

Jhan Piero Rojas Suárez

Keyword(s):

Exposure Time ◽

Seed Quality ◽

Sucrose Solution ◽

Orchid Species ◽

Significant Difference ◽

Physiological Quality ◽

Pre Treatment ◽

Tetrazolium Test

In order to improve the cultivation and conservation of orchid species, it is important to assess the physiological quality of orchid seeds. One of the most traditional tests for analysing seed quality is the tetrazolium test, whose effectiveness is known to improve with pre-treatment of the seeds. As a result, this study compared various pre-treatments to determine how efficient they were at optimising the tetrazolium test in seeds belonging to Cyrtochilum aemulun, Elleanthus aurantiacus and Lephantes sp. The experiment was based on five different pre-treatment solutions, after which the seeds were exposed to tetrazolium at two different concentrations and for three different exposure times. First, the seeds were soaked for ten minutes in one of five pre-treatment solutions: chlorine 0.5%, chlorine 1%, deionised water, sucrose solution 10% (w/v) or a control in which no pre-treatment was applied. After pre-treatment, the seeds were soaked in the dark in a tetrazolium solution, either at 0.25% concentration or at 1.0% concentration, and for either 6, 24 or 48 hours. The control seeds exposed to no pre-treatment showed an increase in the viability of Lephantes sp. at significant difference from the pre-treated seeds. Similarly, pre-treatment with 10% sucrose produced an increase in the tetrazolium test’s effectiveness in E.aurantiacus seeds, with significant differences from the control in most treatments. Therefore, the use of sucrose as a pre-treatment is recommended, in addition to increasing the concentration and exposure time of the seeds.

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