scholarly journals Resistance to red mite in Coffea arabica genotype introgressed with Coffea racemosa genes

Author(s):  
Fernando Cesar Carducci ◽  
Inês Cristina de Batista Fonseca ◽  
Willian Gabriel dos Santos ◽  
Carlos Theodoro Motta Pereira ◽  
Valdir Mariucci Junior ◽  
...  

The aim of this study was to assess the resistance to red mite (Oligonychus ilicis) in Coffea arabica genotypes with introgression of genes from a different species (Coffea racemosa). The experiment was conducted in a greenhouse at IAPAR (Londrina, PR, Brazil), between January 2016 and January 2017. The experiment was conducted in a completely randomized design with five genotypes, 12 replications of one plant per plot. The C. arabica cultivars IPR 100, IPR 99 and IPR 103 were evaluated as well as an F4 Arabica coffee line IAPAR H0113-40-26-10 with introgressed C. racemosa genes. Catuaí Vermelho IAC 99 cultivar, which is a pure C. arabica, was used as a susceptible control. In conditions of high natural infestation of red mite, the resistance of coffee genotypes was evaluated using a standard visual evaluation in January 2017. This evaluation was based on the percentage of leaf area with typical symptoms caused by this mite. Only one genotype, the F4 line IAPAR H0113-40-26-10 showed resistance to the red mite because present less leaf area with symptoms compared to other genotypes. The Arabica coffee cultivars IPR 99, IPR 100 and IPR 103 are as susceptible to red mite as to the susceptible control Catuaí Vermelho IAC 99.

2020 ◽  
pp. 1209-1213
Author(s):  
Melina Marques Holderbaum ◽  
Dhalton Shiguer Ito ◽  
Débora Cristina Santiago ◽  
Luciana Harumi Shigueoka ◽  
Lucas Eduardo Fernandes ◽  
...  

The aim of this study was to evaluate the resistance of Ethiopian Coffea arabica from IAPAR’s germplasm bank to Meloidogyne paranaensis. Two experiments were carried out in a greenhouse in IAPAR, Londrina, Paraná State, Brazil. A completely randomized design was used with 15 treatments, eight replications and one plant per plot. 14 accessions of C. arabica from Ethiopia were evaluated and the cultivar Mundo Novo IAC 376-4 was used as a susceptible control. Seedlings with three to four pairs of leaves were transplanted into plastic cups with a capacity of 700 mL and 1200 eggs and/or J2 of M. paranaensis (IP) were inoculated after one month. The evaluations were performed 130 days after inoculation, when the data of the number of eggs and J2 per gram of roots, the final population of nematodes (FP) were obtained and the reproduction factor (RF) was calculated using the formula: RF = IP / FP. To classify the resistance levels of the genotypes, the reduction of the reproduction factor (RRF) was used. The Ethiopian accessions were classified from highly resistant to highly susceptible. Most of the Ethiopian coffees were highly resistant to M. paranaensis. The genotype Geisha also showed resistance, but is still in heterozygous condition.


Author(s):  
Indra Dwipa ◽  
Merlyana Boangmanalu ◽  
Musliar Kasim ◽  
Dedi Azwardi

Aims: The research aimed to study the effect of several dose of vermicompost to arabica coffee seedling growth. Study Design: Completely randomized design. Place and Duration of Study: The research was conducted in experimental garden of Faculty of Agriculture, Andalas University from September to December 2018. The altitude of research site was 385 meter above sea level (asl). Methodology: The research aimed to study the effect of several dose of vermicompost to arabica coffee seedling growth. Completely randomized design was used in the research that consisted of 5 treatments (No vermicompost, 5 ton/ha, 10 ton/ha, 15 ton/ha and 20 ton/ha) and replied 3 times. Each experimental unit consisted of 6 seedlings. The data was analyzed by Duncan’s New Multiple Test in 5%. Results: The result showed that 5 ton/ha of vermicompost dose has affected the growth component of arabica coffee seedling. Conclusion: The addition of vermicompost dose affected the arabica coffee seedlings growth.


2018 ◽  
Vol 36 (0) ◽  
Author(s):  
C. ALVES ◽  
L. GALON ◽  
R.R. KAIZER ◽  
F.L. WINTER ◽  
C.M. HOLZ ◽  
...  

ABSTRACT: The use of plant species for the phytoremediation of soils contaminated with herbicides is an alternative that has been emphasized to minimize the effects of the persistence of agrochemicals in the environment. The objective of this study was to evaluate the tolerance potential of winter species in soils contaminated with sulfentrazone and fomesafen. The experiment was in a completely randomized design with four replications. Doses of fomesafen (0.0, 0.125, 0.250, and 0.5 kg ha-1) and sulfentrazone (0.0, 0.3, 0.6 and 1.2 kg ha-1) were applied during the pre-emergence of phytoremediate species (black oat, vetch, birdsfood trefoil, radish and lupin). Forty five days after the emergence of the species, the phytotoxicity (%), leaf area (cm2), stalk and/or stem diameter (mm), height (cm) and dry matter (g) variables of the plants were evaluated. Data were submitted to analysis of variance by F test; when significant, linear or non linear regressions were applied to evaluate the effect of herbicide doses on the studied species. Birdsfood trefoil was the less tolerant species to fomesafen and sulfentrazone. Black oat was less affected by the application of fomesafen doses, but it was highly susceptible to sulfentrazone. Radish presented tolerance only up to the fomesafen dose of 0.25 kg ha-1; as for sulfentrazone, the species showed tolerance. The most tolerant species to fomesafen and sulfentrazone, regardless of the dose, was the lupine, which is a possible alternative for the phytoremediation of soils contaminated with these herbicides.


2020 ◽  
pp. 1236-1241
Author(s):  
Gustavo H. Sera ◽  
Filipe G. Carvalho ◽  
Inês C. de B. Fonseca ◽  
Luciana H. Shigueoka ◽  
Santino A. da Silva ◽  
...  

The aim of this study was to prove that Arabica coffee introgressed with C. liberica, have resistance to Meloidogyne paranaensis (Mp). Open pollinated fruits were harvested from mother plants of 29 Arabica coffee genotypes from the IAPAR germplasm bank. Seeds were collected from the fruits and were sown to obtain seedlings to test the resistance to Mp. The experiment was set up in a completely randomized design with 29 coffee genotypes, 8 replications, and one plant per plot. Cultivars Catuaí Vermelho IAC 99 and IPR 100 were used as susceptible and resistant checks, respectively. Seedlings with three to four pairs of leaves were inoculated with 1,400 eggs and juveniles J2 of Mp (IP). At 120 days after inoculation, seedlings were evaluated by counting the nematodes per gram of roots, and the final nematodes population was obtained (FP). The reproduction factor (RF) was calculated using the formula: RF = FP/IP. The reproduction factor reduction was used to classify the resistance levels of genotypes, which were classified as highly resistant (HR), resistant (R), moderately resistant (MR), moderately susceptible (MS), susceptible (S) an highly susceptible (HS). All genotypes differed from Catuaí in resistance factor (RF), five of which did not differ from IPR 100 for RF, and only the line IAPAR 15242 had RF < 1.0. Out of 28 Arabica genotypes introgressed with C. liberica, five HR, 11 R, 11 MR and one MS were identified. However, only IAPAR 15242 and IPR 100 were classified as HR and presented 100% of HR plants, but only the first showed an RF < 1.0. Results revealed that these Arabica coffee genotypes with introgression of C. liberica genes have great potential to be used in breeding programs and they are a new alternative as a source of resistance.


Author(s):  
Rina Arimarsetiowati

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.


Author(s):  
Rina Arimarsetiowati ◽  
Fitria Ardiyani

Plantlet that has developed shoots and roots will have a high level adaptation in the field. The objective of this experiment was to improve the ability of planlet in shooting and rooting so that it is ready for acclimatization in the field. The increase ability in shooting and rooting of the planlet were conducted by adding various types of auxin in the media. The arabica coffee embryo of clone AS 2K which has entered the phase of the cotyledons was transfered into the treatment media containing half-strength of MS (Murashige & Skoog) macro and micro nutrient, vitamin B5, 30 g/L glucose, 100 ml/L coconut water, 50 mg/L AgNO3 added with the combination of IAA, IBA and NAA. The research was conducted by using completely randomized design with seven combined treatment i.e. 0.1 mg/L IBA, 0.1 mg/L NAA, 0.1 mg/L IAA; 0 , 1 mg/L IBA + 0.1 mg/L NAA, 0.1 mg/L IBA + 0.1 mg/L IAA, 0.1 mg/L NAA + 0.1 mg/L IAA; without auxin. There were 12 replications in every treatment and each replication consisted of five cotyledonary embryos. The parameters of observation were the root length, leaf number, leaf area, stem diameter, and height of plantlets. The observations were conducted in eighth weeks after cotyledonary embryo had shoots. The results showed that in the number of leaves and height of planlet parameters, the treatment without auxin was the best result compared to planlet with auxin addition. The addition of auxin varians and their combination did not significantly influent leaf area, root length and stem diameter parameters. The medium tested was optimum for the growth of shoots and roots of AS 2K arabica coffee.


Nativa ◽  
2018 ◽  
Vol 6 (6) ◽  
pp. 600
Author(s):  
Vanessa Cláudia Vasconcelos Segundo ◽  
Renato Innecco ◽  
Ebenézer De Oliveira Silva

O meloeiro tem grande importância econômica para o Nordeste brasileiro. Entretanto, em seu cultivo utiliza-se insumos que podem prejudicar o ambiente; como, os fertilizantes nitrogenados. Objetivou-se avaliar a emissão de N2O em cultivo de meloeiro amarelo ‘Goldex’. O experimento foi instalado em delineamento inteiramente casualizado, com cinco repetições. Os tratamentos foram: 0 (controle), 1,0; 1,5 e 2 vezes a quantidade de nitrogênio recomendada para cada estádio. Foi analisado o fluxo de N2O, área foliar, número de folhas, massa seca da parte aérea, massa seca dos frutos, massa seca da parte radicular, nitrogênio total do solo, das plantas e N (NH4+ e NO3-) do solo. O fluxo de N2O teve comportamento temporal irregular durante a fase de plântulas e crescente na floração. Os tratamentos afetaram significativamente a área foliar, número de folhas, massa seca da parte aérea, massa seca da parte radicular, nitrogênio da parte aérea e parte radicular das plantas e do solo. A aplicação de nitrogênio, em dosagens superiores à recomendada, não aumentou a produção do meloeiro. E o cálculo do fator de emissão mostrou que a emissão do N2O não atingiu valores prejudiciais ao ambiente.Palavras-chave: fator de emissão, efeito estufa e nitrogênio. NITROUS OXIDE EMISSION ASSOCIATED WITH UREA USE THE YELLOW MELON CROP ABSTRACT: The melon has great economic importance for the Brazilian Northeast. However, in its cultivation uses inputs that can harm the environment; Such as nitrogen fertilizers. The objective of this study was to evaluate the emission of N2O in yellow 'Goldex' cultivar. The experiment was installed in a completely randomized design with five replications. The treatments were: 0 (control), 1.0; 1.5 and 2 times the amount of nitrogen recommended for each stage. N2O flux, leaf area, leaf number, shoot dry mass, fruit dry mass, dry mass of the root portion, total soil nitrogen, of the plants and N (NH4 + and NO3 -) of the soil were analyzed. The flow of N2O had irregular temporal behavior during the seedling phase and increased in flowering. The treatments affected significantly the leaf area, number of leaves, dry mass of the aerial part, dry mass of the root part, nitrogen of the aerial part and root part of the plants and the soil. The application of nitrogen, in dosages higher than the recommended one, did not increase the production of the melon. And the calculation of the emission factor showed that the emission of N2O did not reach values harmful to the environment.Keywords: emission fator, greenhouse and nitrogen.


2018 ◽  
Vol 7 (1) ◽  
pp. 6
Author(s):  
Hairin Juanda ◽  
Tutik Nugrahini ◽  
Mahdalena Mahdalena

Effect of NASA Liquid Organic Fertilizer and Compost Fertilizer on Kenaf Plant Growth (Hibiscus cannabinus L). This study aims to determine the effect of NASA POC, compost fertilizer and the interaction of NASA POC and compost fertilizer on kenaf plants. This research was carried out at East Borneo Samarinda Indonesia. with a time of ± 4 months, starting from June to September 2016. The design of the study uses factorial completely randomized design (CRD) consisting of 2 treatments and 4 replications. The treatment consists of 2 factors. The first factor is the administration of NASA POC consisting of 4 levels, namely: j0: Control (without treatment), j1: POC with a concentration of 10 ml / liter of water, j2: POC with a concentration of 15 ml / liter of water, j3: POC with a concentration of 20 ml / liter of water. The second factor is Compost fertilizer consisting of 4 levels, namely: k0: Without the provision of Compost Fertilizer, k1: Composting 500 gram / polybag, k2: Composting Fertilizer 700 grams / polybag, k3: Composting 800 grams / polybag. From the analysis of variance showed that the effect of giving NASA POC (J), compost (K) and the interaction of NASA POC and compost (JK) did not affect the parameters of plant height 30 DAP, 60 DAP and 90 DAP, stem diameter 30 DAP, 60 DAP and 90 DAP, number of leaves 30 DAP, 60 DAP and 90 DAP, leaf area 30 DAP, 60 DAP and 90 DAP. 


2020 ◽  
Vol 41 (4) ◽  
pp. 1151
Author(s):  
André Ricardo Zeist ◽  
Juliano Tadeu Vilela de Resende ◽  
Daniel Suek Zanin ◽  
Israel Felipe Lustosa da Silva ◽  
Daniel Fernandes da Silva ◽  
...  

This study aimed to estimate plastochron, leaf area, and yield of Physalis peruviana seedlings grown in different environments and transplanted on different dates. The experiment was carried out in a completely randomized design, arranged in a 2 x 3 factorial scheme, namely: two cultivation environments (protected and field) and three transplanting dates (10/27/2013, 12/15/2013, and 02/11/2014). Four replicates of eight plants were used per plot, and the four central plants composed the useful area. Based on mathematical models and temperature data, plastochron was estimated for the entire crop cycle and vegetative and reproductive phases separately. Leaf area and node number were measured, and their relationship established at the end of the study. Other variables assessed were fruit number, mass, and yield per plant. Our results showed that the higher temperatures in the protected environment anticipated phenological timings. Plastochron values of 60.0 and 16.5 °C day-1 can be used during vegetative and reproductive stages, respectively. The protected environment increased vegetative growth and development, as well as yield of P. peruviana seedlings. In the Guarapuava-PR region, P. peruviana seedlings should be transplanted during the spring so that higher fruit yield and quality could be achieved.


2016 ◽  
Vol 31 (3) ◽  
pp. 231
Author(s):  
Adriano Oliveira Nunes ◽  
Rodolfo Belo Exler ◽  
Fábio Macêdo Nunes ◽  
Luis Cesar Paulillo

O presente trabalho teve por objetivo caracterizar os sintomas de deficiências de macro e micro nutrientes em mudas de pinhão-manso (Jatropha curcas). O experimento foi conduzido em um viveiro instalado na Faculdade de Tecnologia e Ciência (FTC), Salvador – Ba. As mudas foram submetidas à solução nutritiva completa (tratamento testemunha) e soluções com omissão dos nutrientes N, P, K, Ca, Mg, S, B, Cu, Fe, Mn, Zn, Mo que corresponderam aos demais tratamentos, que caracterizam a  técnica do elemento faltante. As sementes foram plantadas em vasos de plástico com capacidade de 2 Kg, numa profundidade de 5 cm, cobertas com camada fina de areia lavada, as condições de luminosidade foram de 50% em viveiro com cobertura de tela sombrite preta. Adotou-se o delineamento experimental inteiramente casualizado com treze tratamentos e nove repetições. Foram avaliados os sintomas visuais de deficiência de nutrientes aos 45 dias após a aplicação dos tratamentos. As plantas apresentaram sintomas de deficiências minerais comuns da espécie. Os sintomas apresentados foram principalmente observados no tratamento com omissão dos elementos N, P, K, Ca, Mg, S, B e Mo. Todos os tratamentos com ausência de macronutrientes apresentaram alterações visuais de deficiência nutricional, no entanto somente os tratamentos com omissão de B e Mn apresentaram sintomas decorrentes da omissão de micronutrientes.Palavras-chave: Jatropha curcas, macronutrientes, micronutrientes, diagnose visual. VISUAL EVALUATION OF MACRO AND MICRO NUTRIENTS DEFICIENCIES SYMPTOMS IN JATROPHA SEEDLINGS IN FUNCTION OF DIFFERENT NUTRITION DOSES ABSTRACT: This study aimed to make a visual evaluation of the growth and characterization of the symptoms of macro and micro nutrient deficiencies in jatropha seedlings (Jatropha curcas). The experiment was conducted in a vivarium at the Faculty of Technology and Science (FTC), Salvador - Ba. The seedlings were treated to the complete nutrient solution (standard treatment) and also solutions with omissions of nutrients N, P, K, Ca, Mg, S, B, Cu, Fe, Mn, and Zn, Mo, that corresponded to other treatments featuring to using the missing element technique. Seeds were planted at a depth of 5 cm and covered with a thin layer of washed sand. It adopted a completely randomized design with thirteen treatments and nine repetitions. The symptoms of mineral deficiencies were evaluated over a period of 45 days. The plants had symptoms of common mineral deficiencies of the species. The symptoms were observed in the absence of treatment with elements N, P, K, Ca, Mg, S, B and Mo. The results showed that all treatments with omission of macronutrients presented visual alterations of nutritional deficiency, however only the treatments with omission of B and Mn showed symptoms of the subtraction of micronutrients.Keywords: Jatropha curcas, macronutrient, micronutrient, visual symptoms.


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