scholarly journals Pengaruh penambahan auxin terhadap pertunasan dan perakaran kopi arabika perbanyakan Somatic Embryogenesis (The effects of shooting and rooting of arabica coffee propagation through Embryogenesis Somatic auxin uses).

Author(s):  
Rina Arimarsetiowati ◽  
Fitria Ardiyani

Plantlet that has developed shoots and roots will have a high level adaptation in the field. The objective of this experiment was to improve the ability of planlet in shooting and rooting so that it is ready for acclimatization in the field. The increase ability in shooting and rooting of the planlet were conducted by adding various types of auxin in the media. The arabica coffee embryo of clone AS 2K which has entered the phase of the cotyledons was transfered into the treatment media containing half-strength of MS (Murashige & Skoog) macro and micro nutrient, vitamin B5, 30 g/L glucose, 100 ml/L coconut water, 50 mg/L AgNO3 added with the combination of IAA, IBA and NAA. The research was conducted by using completely randomized design with seven combined treatment i.e. 0.1 mg/L IBA, 0.1 mg/L NAA, 0.1 mg/L IAA; 0 , 1 mg/L IBA + 0.1 mg/L NAA, 0.1 mg/L IBA + 0.1 mg/L IAA, 0.1 mg/L NAA + 0.1 mg/L IAA; without auxin. There were 12 replications in every treatment and each replication consisted of five cotyledonary embryos. The parameters of observation were the root length, leaf number, leaf area, stem diameter, and height of plantlets. The observations were conducted in eighth weeks after cotyledonary embryo had shoots. The results showed that in the number of leaves and height of planlet parameters, the treatment without auxin was the best result compared to planlet with auxin addition. The addition of auxin varians and their combination did not significantly influent leaf area, root length and stem diameter parameters. The medium tested was optimum for the growth of shoots and roots of AS 2K arabica coffee.

Author(s):  
Antonio F. Monteiro Filho ◽  
Márcia R. Q. A. Azevedo ◽  
Carlos A. V. de Azevedo ◽  
Josely D. Fernandes ◽  
Carisa R. da Silva ◽  
...  

ABSTRACT The objective of this study was to evaluate the growth of crisp lettuce in hydroponic cultivation, following the nutrient film technique (NFT), using optimized mineral and organomineral nutrient solutions. The experiment was set in 8 x 3 factorial scheme, with treatments distributed in randomized blocks and split plots, with three replicates. The plots corresponded to eight nutrient solutions, of which four were mineral solutions with chemical composition suggested by Bernardes, Furlani, Castellane & Araújo and Ueda, and four were organomineral solutions, suggested by this research with chemical composition similar to those of the previously mentioned mineral solutions. The subplots corresponded to three varieties of crisp lettuce: Thaís, Vanda and Verônica. At 25 days after transplanting, the following parameters were evaluated: stem and crown diameter, root length, leaf area and number of leaves. For stem and crown diameters and number of leaves, the mineral solutions promoted the highest means. The mineral solution of Furlani and the organomineral solutions, except the modified solution of Castellane & Araújo, promoted the greatest root lengths. The smallest leaf area was obtained in the organomineral solution of Ueda. The cultivar Verônica had the highest root length and stem diameter.


2018 ◽  
Vol 7 (1) ◽  
pp. 6
Author(s):  
Hairin Juanda ◽  
Tutik Nugrahini ◽  
Mahdalena Mahdalena

Effect of NASA Liquid Organic Fertilizer and Compost Fertilizer on Kenaf Plant Growth (Hibiscus cannabinus L). This study aims to determine the effect of NASA POC, compost fertilizer and the interaction of NASA POC and compost fertilizer on kenaf plants. This research was carried out at East Borneo Samarinda Indonesia. with a time of ± 4 months, starting from June to September 2016. The design of the study uses factorial completely randomized design (CRD) consisting of 2 treatments and 4 replications. The treatment consists of 2 factors. The first factor is the administration of NASA POC consisting of 4 levels, namely: j0: Control (without treatment), j1: POC with a concentration of 10 ml / liter of water, j2: POC with a concentration of 15 ml / liter of water, j3: POC with a concentration of 20 ml / liter of water. The second factor is Compost fertilizer consisting of 4 levels, namely: k0: Without the provision of Compost Fertilizer, k1: Composting 500 gram / polybag, k2: Composting Fertilizer 700 grams / polybag, k3: Composting 800 grams / polybag. From the analysis of variance showed that the effect of giving NASA POC (J), compost (K) and the interaction of NASA POC and compost (JK) did not affect the parameters of plant height 30 DAP, 60 DAP and 90 DAP, stem diameter 30 DAP, 60 DAP and 90 DAP, number of leaves 30 DAP, 60 DAP and 90 DAP, leaf area 30 DAP, 60 DAP and 90 DAP. 


2017 ◽  
Vol 8 (1) ◽  
pp. 29 ◽  
Author(s):  
Imanuel Jhon Hansen

The aim of research to determine the effect of compost main pod husks and dolomite as well as their interaction on the growth of cocoa seedlings in media ultisol. Research conducted at the Experimental Farm, Faculty of Agriculture, University of Riau from November 2015 until February 2016. Research in the form of factorial experiment using completely randomized design (CRD). The first factor pod husks compost comprises four levels (0, 75, 100 and 125 g / polybag) and the second factor dolomite consists of three levels (0, 10, and 20 g / polybag), each combination treatment was repeated 3 times. Parameters measured were plant height, leaf number, stem diameter, and leaf area. Each parameter the observed data were statistically analyzed using analysis of variance and test of Duncan's New Multiple Range Test (DNMRT) at 5% level. The results showed the effect of compost pod husks dose of 125 g / polybag significantly increased plant height, stem diameter, number of leaves and leaf area while dolomite dose of 20 g / polybag no real effect except leaf area compared with no treatment. Interaction pod husks compost dose of 100 g / polybag and dolomite dose of 20 g / polybag increase stem diameter, number of leaves and leaf area significantly compared without compost pod husks and dolomite but less tangible than other combinations.


Nabatia ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Nur Azizatu Rohmah

This study aims to determine the effect of giving various kinds and duration of ZPT immersion on the growth of tin plant cuttings (Ficus carica L.). This research was carried out in October - December 2020, at the Terdana Village, Pondok Kubang District, Central Bengkulu Regency. This study used a Factorial Completely Randomized Design (CRD) with two factors, the first factor was giving various PGR (Z) which consisted of Control, Rootone-F, Shallot Solution, Bamboo Shoots Solution, while the second factor was the duration of immersion of PGR which consisted of of 12 hours, 18 hours, 24 hours, each treatment was repeated 3 times. The results of the study were analyzed using Variety Analysis and if it had a significant effect, further tests were carried out using Duncan's Multiple Range Test (DMRT) level 5%. The results showed that there was an interaction between the administration of Rootone-F and the immersion time of ZPT 12 to 24 hours on the percentage of live cuttings of Tin (Ficus carica L.) plant. 12 WAP, root length, percentage of live cuttings and leaf area on cuttings growth of Tin (Ficus carica L.) PGR immersion time treatment was not able to increase shoot length 12 WAP, number of shoots 12 WAP, number of leaves 12 WAP, root length, percentage of cuttings Life and leaf area on the growth of cuttings of Tin (Ficus carica L.) It is recommended to increase the concentration of natural PGR so that it can be used as a substitute for Rootone-F.


2021 ◽  
Vol 232 ◽  
pp. 03024
Author(s):  
Agus Miftakhurrohmat ◽  
Sutarman

This study aims to determine the effect of bacterial isolates of Bacillus subtilis Bs-Sdj-01 and Trichoderma sp. Tc-Jro-02 isolates as biological fertilizer in plant growth until the end of the vegetative phase. The experiment was arranged factorially in a Completely Randomized Design (CRD) with each factor being the application of B. subtilis consisting of and without B. subtilis and the Trichoderma application consisting of with and without Trichoderma; the experiment was repeated four times. The observed variables were: plant height, stem diameter, number of leaves, leaf area, stover dry weight, root dry weight, and B. subtilis population at the end of the observation. Data were analyzed using with ANOVA and HSD tests at the 5% level. The combination of Trichoderma sp. and B. subtilis bacteria produce the highest increase in plant height, stem diameter, number of leaves, leaf area, stover dry weight, and root dry weight of soybean plants up to 35 days after planting and increase B. subtilis population grew from 1010 CFU.g-1 to be 4.43x1011 CFU.g-1. The presence of Trichoderma supports the activity of B. subtilis in the rhizosphere of the Detam variety soybean plantation.


2021 ◽  
Vol 26 (4) ◽  
pp. 591-596
Author(s):  
Hidayati Fatchur Rochmah ◽  
Restu Puji Mumpuni ◽  
Dian Eka Ramadhani

This study evaluates the feasibility of integration of the single-bud sugarcane and catfish farming system and the growth rate of both plants and fish. The observation started from September to November 2020. The design used was randomized with one factor. The treatments used were cultivation of single-bud sugarcane in polybags, hydroponic single-bud sugarcane cultivation, integration of single-bud sugarcane-catfish, and catfish cultivation. The results showed that the growth of sugarcane seedlings and the catfish indicate that the integrated cultivation system of sugarcane-catfish is feasible to apply. Such an integration system produces a better number of leaves, stem diameter, leaf area, and root length than the cultivation system of sugarcane seedlings in polybags. The weight of the canopy and the root of sugarcane seedlings in the integration system is also noticeable greater than the sugarcane seedlings cultivated in the polybag. In addition, the rate of catfish growth in the integration system is better than catfish's ordinary cultivation.   Keywords: hydroponic, integration farming system, single bud sugarcane planting


2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 


Author(s):  
Budiman Budiman ◽  
Natsir Sandiah ◽  
La Malesi

This study aimed to determine the best dosage of goat manure as a natural fertilizer on Beha grass(Brachiaria humidicola). This study used 64 poles of Beha grass grown in polybags divided into 16plots, and it used a completely randomized design (CRD) to analyze the data. This study consist of 4treatments that is P0 (0 ton/ha of goat manure), P1 (10 ton/ha of goat manure), P2 (15 ton/ha of goatmanure), and P3 (20 ton/ha of goat manure). This study's variables were plant height, number of leaves,numbers of tillers, new production, and root length. Data analysis used ANOVA variance continued withreal honest difference test. The result of this study showed that averages of plant height (cm) wereP0=26, P1=50.5, P2=51.5, and P3=52.75. Averages of the number of leaves were P0=22, P1=110,P2=160, and P3=185. Averages of numbers of tillers were P0=7, P1=25, P2=37, and P3=38. Averages ofnew production (g) were P0=17.75, P1=123.5, P2=178, and P3=192.25. Averages of root length (cm)were P0=37.8, P1=39.5, P2=41.775, and P3=49.5. Fertilization with natural fertilizer from goat manurehas a genuine effect (P<0.01) on the growth of Beha grass. The best dosage of fertilization with goatmanure was 15 ton/ha.


2018 ◽  
Vol 36 (0) ◽  
Author(s):  
C. ALVES ◽  
L. GALON ◽  
R.R. KAIZER ◽  
F.L. WINTER ◽  
C.M. HOLZ ◽  
...  

ABSTRACT: The use of plant species for the phytoremediation of soils contaminated with herbicides is an alternative that has been emphasized to minimize the effects of the persistence of agrochemicals in the environment. The objective of this study was to evaluate the tolerance potential of winter species in soils contaminated with sulfentrazone and fomesafen. The experiment was in a completely randomized design with four replications. Doses of fomesafen (0.0, 0.125, 0.250, and 0.5 kg ha-1) and sulfentrazone (0.0, 0.3, 0.6 and 1.2 kg ha-1) were applied during the pre-emergence of phytoremediate species (black oat, vetch, birdsfood trefoil, radish and lupin). Forty five days after the emergence of the species, the phytotoxicity (%), leaf area (cm2), stalk and/or stem diameter (mm), height (cm) and dry matter (g) variables of the plants were evaluated. Data were submitted to analysis of variance by F test; when significant, linear or non linear regressions were applied to evaluate the effect of herbicide doses on the studied species. Birdsfood trefoil was the less tolerant species to fomesafen and sulfentrazone. Black oat was less affected by the application of fomesafen doses, but it was highly susceptible to sulfentrazone. Radish presented tolerance only up to the fomesafen dose of 0.25 kg ha-1; as for sulfentrazone, the species showed tolerance. The most tolerant species to fomesafen and sulfentrazone, regardless of the dose, was the lupine, which is a possible alternative for the phytoremediation of soils contaminated with these herbicides.


2018 ◽  
Vol 8 (2) ◽  
pp. 239-246 ◽  
Author(s):  
Derblai Casaroli ◽  
Flávio De Oliveira Rosa ◽  
José Alves Júnior ◽  
Adão Wagner Pêgo Evangelista

In the searching of forest species with good wood quality, easy adaptability and high commercial value, the African mahogany (Khaya ivorensis Chev. A.) have been a good alternative. This study aimed evaluate the phenometric responses of African Mahogany submitted to irrigation. The field experiment was carried out in Bonfinópolis-GO, Brazil, with young plants of African mahogany, being 5 irrigated and 5 non-irrigated. The following variables were evaluated: plant height, stem heigh, number of leaves and leaflets, stem diameter and leaf area. A completely randomized design was used in a bi-factorial scheme 2x9 (A: irrigated and non-irrigated; D: 0, 120, 180, 240, 300, 360, 420, 480, 540 days after transplanting). All the variables showed significant differences not only for irrigated and non-irrigated treatments but also for the times of evaluation, except for stem diameter. Irrigation resulted in higher growth of plants and the irrigation of young African mahogany plants should be used mainly in drought period.


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