scholarly journals Unveiling the Bacterial Sesquiterpenome of Streptomyces sp. CBMAI 2042 Discloses Cyclases with Versatile Performances

2022 ◽  
Author(s):  
Douglas Sachito ◽  
Luciana de Oliveira
Keyword(s):  
Mass Spectrometry ◽  
Industrial Applications ◽  
Farnesyl Diphosphate ◽  
Gas Chromatography Mass Spectrometry ◽  
Streptomyces Sp ◽  
Genome Data ◽  
Bulk Chemicals ◽  
Terpene Cyclase ◽  
Sesquiterpene Cyclase ◽  
Volatile Terpenes

Terpenes are the most abundant class of natural product that exist in nature. They possess a myriad of industrial applications including pharmaceutical, perfumery and flavors, bulk chemicals, and fuel. Intriguingly, until today, the vast majority of characterized terpenoids have been isolated from plants and fungi, and only in recent years bacteria were found to generate a representative reservoir of terpenoids molecules. Mining Streptomyces sp. CBMAI 2042 genome data has revealed the presence of five terpene cyclase genes. Chemical analysis of mycelium extract of this bacteria strain has unveiled at least 28 volatile terpenes molecules, where three encoding sesquiterpene cyclase (STC) genes are apparently responsible for their biosynthesis. The cyclic products obtained by incubation of these three purified recombinant STCs with farnesyl diphosphate (FPP) were analyzed by gas chromatography-mass spectrometry (GC-MS) and identified using the Van den Dool and Kratz equation.

scholarly journals Enhanced Production of Photosynthetic Pigments and Various Metabolites and Lipids in the Cyanobacteria Synechocystis sp. PCC 7338 Culture in the Presence of Exogenous Glucose

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 214
Author(s):  
YuJin Noh ◽  
Hwanhui Lee ◽  
Myeongsun Kim ◽  
Seong-Joo Hong ◽  
Hookeun Lee ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Growth ◽  
Photosynthetic Pigments ◽  
Industrial Applications ◽  
Gas Chromatography Mass Spectrometry ◽  
Enhanced Production ◽  
Exogenous Glucose ◽  
Nanoelectrospray Ionization ◽  
Lipid Species ◽  
Synechocystis Sp

Synechocystis strains are cyanobacteria that can produce useful biomaterials for biofuel and pharmaceutical resources. In this study, the effects of exogenous glucose (5-mM) on cell growth, photosynthetic pigments, metabolites, and lipids in Synechocystis sp. PCC 7338 (referred to as Synechocystis 7338) were investigated. Exogenous glucose increased cell growth on days 9 and 18. The highest production (mg/L) of chlorophyll a (34.66), phycocyanin (84.94), allophycocyanin (34.28), and phycoerythrin (6.90) was observed on day 18 in Synechocystis 7338 culture under 5-mM glucose. Alterations in metabolic and lipidomic profiles under 5-mM glucose were investigated using gas chromatography-mass spectrometry (MS) and nanoelectrospray ionization-MS. The highest production (relative intensity/L) of aspartic acid, glutamic acid, glycerol-3-phosphate, linolenic acid, monogalactosyldiacylglycerol (MGDG) 16:0/18:1, MGDG 16:0/20:2, MGDG 18:1/18:2, neophytadiene, oleic acid, phosphatidylglycerol (PG) 16:0/16:0, and PG 16:0/17:2 was achieved on day 9. The highest production of pyroglutamic acid and sucrose was observed on day 18. We suggest that the addition of exogenous glucose to Synechocystis 7338 culture could be an efficient strategy for improving growth of cells and production of photosynthetic pigments, metabolites, and intact lipid species for industrial applications.

scholarly journals Isolation, Purification and Application of Secondary Metabolites From Lichen Parmelia Perlata

2017 ◽  
Vol 14 (4) ◽  
pp. 1413-1428 ◽  
Author(s):  
K. Leela ◽  
C. Anchana Devi
Keyword(s):  
Mass Spectrometry ◽  
Secondary Metabolites ◽  
Industrial Applications ◽  
Antidiabetic Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Symbiotic Association ◽  
Potential Source ◽  
Fungal Partner ◽  
Solvent Systems ◽  
Layer Chromatography

ABSTRACT: Lichens are composite algae having a symbiotic association with a fungal partner. They produce numerous secondary metabolites, which play an important role in pharmaceutical and in other industrial applications. The Secondary metabolites produced by lichens are found to be 80% more when compared to that produced by other organisms. Not much work has been carried out on lichens due to the difficulty in their cultivation but still it emerges as a potential source in developing therapeutically important drugs which are widely beneficial in all fields of application. The Present study was aimed to isolate, purify and determine the applications of secondary metabolites from Lichen Parmelia perlata. The presence of these compounds were detected and purified by thin layer chromatography and column chromatography using specific solvent systems. The purified fractions were then identified by Gas chromatography-Mass spectrometry (GC-MS). The compounds were then subjected to application oriented studies such as antimicrobial activity, antioxidant activity and antidiabetic activity. Not much work have been carried out on the isolation of a specific glycoside and alkaloid compound from Lichen Parmelia perlata, so this study was an attempt to explore the applications of these individual compounds which could prove beneficial to the mankind for different purposes.

Sign in / Sign up

Export Citation Format

Share Document