scholarly journals Isolation, Purification and Application of Secondary Metabolites From Lichen Parmelia Perlata

2017 ◽  
Vol 14 (4) ◽  
pp. 1413-1428 ◽  
Author(s):  
K. Leela ◽  
C. Anchana Devi
Keyword(s):  
Mass Spectrometry ◽  
Secondary Metabolites ◽  
Industrial Applications ◽  
Antidiabetic Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Symbiotic Association ◽  
Potential Source ◽  
Fungal Partner ◽  
Solvent Systems ◽  
Layer Chromatography

ABSTRACT: Lichens are composite algae having a symbiotic association with a fungal partner. They produce numerous secondary metabolites, which play an important role in pharmaceutical and in other industrial applications. The Secondary metabolites produced by lichens are found to be 80% more when compared to that produced by other organisms. Not much work has been carried out on lichens due to the difficulty in their cultivation but still it emerges as a potential source in developing therapeutically important drugs which are widely beneficial in all fields of application. The Present study was aimed to isolate, purify and determine the applications of secondary metabolites from Lichen Parmelia perlata. The presence of these compounds were detected and purified by thin layer chromatography and column chromatography using specific solvent systems. The purified fractions were then identified by Gas chromatography-Mass spectrometry (GC-MS). The compounds were then subjected to application oriented studies such as antimicrobial activity, antioxidant activity and antidiabetic activity. Not much work have been carried out on the isolation of a specific glycoside and alkaloid compound from Lichen Parmelia perlata, so this study was an attempt to explore the applications of these individual compounds which could prove beneficial to the mankind for different purposes.

scholarly journals Enhanced Production of Photosynthetic Pigments and Various Metabolites and Lipids in the Cyanobacteria Synechocystis sp. PCC 7338 Culture in the Presence of Exogenous Glucose

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 214
Author(s):  
YuJin Noh ◽  
Hwanhui Lee ◽  
Myeongsun Kim ◽  
Seong-Joo Hong ◽  
Hookeun Lee ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Growth ◽  
Photosynthetic Pigments ◽  
Industrial Applications ◽  
Gas Chromatography Mass Spectrometry ◽  
Enhanced Production ◽  
Exogenous Glucose ◽  
Nanoelectrospray Ionization ◽  
Lipid Species ◽  
Synechocystis Sp

Synechocystis strains are cyanobacteria that can produce useful biomaterials for biofuel and pharmaceutical resources. In this study, the effects of exogenous glucose (5-mM) on cell growth, photosynthetic pigments, metabolites, and lipids in Synechocystis sp. PCC 7338 (referred to as Synechocystis 7338) were investigated. Exogenous glucose increased cell growth on days 9 and 18. The highest production (mg/L) of chlorophyll a (34.66), phycocyanin (84.94), allophycocyanin (34.28), and phycoerythrin (6.90) was observed on day 18 in Synechocystis 7338 culture under 5-mM glucose. Alterations in metabolic and lipidomic profiles under 5-mM glucose were investigated using gas chromatography-mass spectrometry (MS) and nanoelectrospray ionization-MS. The highest production (relative intensity/L) of aspartic acid, glutamic acid, glycerol-3-phosphate, linolenic acid, monogalactosyldiacylglycerol (MGDG) 16:0/18:1, MGDG 16:0/20:2, MGDG 18:1/18:2, neophytadiene, oleic acid, phosphatidylglycerol (PG) 16:0/16:0, and PG 16:0/17:2 was achieved on day 9. The highest production of pyroglutamic acid and sucrose was observed on day 18. We suggest that the addition of exogenous glucose to Synechocystis 7338 culture could be an efficient strategy for improving growth of cells and production of photosynthetic pigments, metabolites, and intact lipid species for industrial applications.

scholarly journals Photorhabdus sp. ETL Antimicrobial Properties and Characterization of Its Secondary Metabolites by Gas Chromatography–Mass Spectrometry

Life ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 787
Author(s):  
Tshikala Eddie Lulamba ◽  
Ezekiel Green ◽  
Mahloro Hope Serepa-Dlamini
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Secondary Metabolites ◽  
Pathogenic Bacteria ◽  
Insect Pests ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Toxigenic Fungi ◽  
Properties And Characterization

Entomopathogenic nematodes (EPNs) are known to be highly pathogenic to insect pests, due to their associated symbiotic bacteria, which produce virulence factors, exo-enzymes and other harmful secondary metabolites to conquer, kill, and degrade their insect hosts. However, these properties are not fully characterized. This study reports on the antimicrobial activities of Photorhabdus sp. strain ETL, symbiotically associated to an insect pathogenic nematode, Heterorhabditis zealandica, against human pathogenic bacteria and toxigenic fungi, as well as the non-targeted profiling of its secondary metabolites (SMs) using gas chromatography coupled to high-resolution time-of-flight mass spectrometry. Fatty acids including 3-eicosene, (E)-; 5-eicosene, (E)-; eicosene; 9-octadecenamide; undecanoic acid with shown antimicrobial activities were detected. This provided more insight on the composition and bioactivities of SMs produced by the Photorhabdus sp.

Thin-layer chromatography and gas chromatography-mass spectrometry examination of shoe materials from patients with shoe dermatitis

2015 ◽  
Vol 72 (4) ◽  
pp. 248-252 ◽  
Author(s):  
Sri A. Febriana ◽  
Erik Zimerson ◽  
Cecilia Svedman ◽  
Winarto Haryadi ◽  
Pieter-Jan Coenraads ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Layer Chromatography

Extraction and Characterization of Gibberellins from Hordeum vulgare L. Seedlings

1974 ◽  
Vol 1 (2) ◽  
pp. 183 ◽  
Author(s):  
KF Faull ◽  
BG Coombe ◽  
LG Paleg
Keyword(s):  
Mass Spectrometry ◽  
Dry Weight ◽  
Paper Electrophoresis ◽  
The Other ◽  
Gas Chromatography Mass Spectrometry ◽  
Gas Liquid Chromatography ◽  
Fresh Weight ◽  
Hordeum Vulgare L ◽  
Layer Chromatography

Two gibberellins, one GA1-like, the other GA3-like, were identified in the extracts of roots and tops of 8-,11- and 15-day-old barley seedlings by paper chromatography, paper electrophoresis, thin-layer chromatography, gas-liquid chromatography and bioassay procedures, followed by combined gas chromatography-mass spectrometry. The amounts of gibberellins in the seedlings ranged from 7 to 11 ng per plant. The concentrations of gibberellins in the seedlings were 32-320 ng/g dry weight and 5-28 ng/g fresh weight; concentrations in the roots were higher than those in the shoots.

Efficient extraction of basic, neutral, and weakly acidic drugs from plasma for analysis by gas chromatography-mass spectrometry

1991 ◽  
Vol 37 (11) ◽  
pp. 1975-1978 ◽  
Author(s):  
K E Brooks ◽  
N B Smith
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Acetic Anhydride ◽  
Mobile Phase ◽  
Plasma Sample ◽  
Gas Chromatography Mass Spectrometry ◽  
Acidic Drugs ◽  
Chromatography Mass Spectrometry ◽  
Efficient Extraction ◽  
Layer Chromatography

Abstract We describe a method for efficiently extracting basic, neutral, and weakly acidic drugs from plasma for toxicological analysis by gas chromatography-mass spectrometry (GC/MS). The 2-mL plasma sample is diluted with an equal volume of saturated NaCl containing triethylamine, 10 mmol/L, and then extracted twice with 4 mL of an equivolume solution of dichloromethane/acetone. The organic (top) phases are combined, then mixed with 1 mL of water, 200 mg of NaHCO3, and 100 microliters of acetic anhydride. This mixture is then heated at 75 degrees C until the solvents have boiled off and aqueous acetylation is complete (less than 30 min). After addition of 1 mL of water and 2 g of NaCl, the sample is extracted twice with 2 mL of dichloromethane/acetone (2/1 by vol). The combined extracts are dried and then subjected to thin-layer chromatography on a blank Toxi-Lab Toxi-A chromatogram with 1-chlorobutane as the developing solvent (about 6 min). After the lipids have migrated with the mobile phase, the drugs are eluted from the origin with acetone/triethylamine (29/1 by vol), evaporated, and reconstituted in injection solvent. With this procedure drugs are recovered relatively quickly (less than 2 h) and the GC/MS total ion chromatograms are very clean. Studies with 13 basic, neutral, and weakly acidic drugs showed that all except theophylline were extracted with recoveries of at least 75%.

Gas Chromatographic Determination of Low Levels of Di-(2-ethylh exyl)phthalate in Soy Oil

1973 ◽  
Vol 56 (1) ◽  
pp. 181-183
Author(s):  
D T Williams
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chromatographic Determination ◽  
Phthalate Ester ◽  
Gas Chromatography Mass Spectrometry ◽  
Soy Oil ◽  
Chromatography Mass Spectrometry ◽  
Cleanup Procedure ◽  
Layer Chromatography ◽  
Ethylhexyl Phthalate

Abstract Di-(2-ethylhexyl)phthalate can be determined by gas chromatography at a level of 0.3 ppm in soy oil. A cleanup procedure involving epoxidation of soy oil residues with m-chloroperbenzoic acid allows confirmation of the phthalate ester by thin layer chromatography-mass spectrometry or gas chromatography-mass spectrometry.

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