Impact of Chronic Tramadol Intake on Reproductive Hormones and Related Histopathological Changes in Adult Albino Rats

2020 ◽  
Vol 35 (2) ◽  
pp. 1-13
Author(s):  
Mona Moussa ◽  
Nahla Tolba ◽  
Soha Ashry, ◽  
Asmaa Ali ◽  
Suzi Atallah
Keyword(s):  
Reproductive Hormones ◽  
Albino Rats ◽  
Histopathological Changes

scholarly journals Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Godwin Adakole Ujah ◽  
Victor Udo Nna ◽  
Joseph Bagi Suleiman ◽  
Chinedum Eleazu ◽  
Chukwuemeka Nwokocha ◽  
...  
Keyword(s):  
Body Weight ◽  
Sperm Count ◽  
Reproductive Hormones ◽  
Nuclear Antigen ◽  
Albino Rats ◽  
Target Cells ◽  
Negative Effects ◽  
Related Proteins ◽  
Proliferating Cell ◽  
Cancerous Cells

AbstractDoxorubicin (DOX) is a broad-spectrum chemotherapeutic drug used in the treatment of cancers. It acts by generating reactive oxygen species in target cells. The actions are, however, not limited to cancerous cells as it attacks healthy cells, killing them. This study investigated the benefits of the antioxidant, tert-butylhydroquinone (tBHQ), on testicular toxicity following DOX therapy. Twenty-four adult male albino rats were assigned randomly into four groups (n = 6), namely: normal control (NC), tBHQ, DOX and tBHQ + DOX groups. tBHQ (50 mg/kg body weight in 1% DMSO) was administered orally for 14 consecutive days, while a single DOX dose (7 mg/kg body weight) was administered intraperitoneally on Day 8. DOX decreased sperm count, motility and viability, and decreased the levels of steroidogenesis-related proteins, and reproductive hormones. Furthermore, DOX decreased the expression of antioxidant cytoprotective genes, and decreased the protein level of proliferating cell nuclear antigen in the testis. Conversely, DOX increased the expression of pro-inflammatory and pro-apoptotic genes in the testis. These negative effects were ameliorated following the intervention with tBHQ. Our results suggest that tBHQ protects the testis and preserves both steroidogenesis and spermatogenesis in DOX-treated rats through the suppression of oxidative stress, inflammation and apoptosis.

scholarly journals Histopathological Study of Cyclosporine Pulmonary Toxicity in Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Said Said Elshama ◽  
Ayman El-Meghawry EL-Kenawy ◽  
Hosam-Eldin Hussein Osman
Keyword(s):  
Body Weight ◽  
Periodic Acid ◽  
Physiological Saline ◽  
Immunosuppressive Drugs ◽  
Albino Rats ◽  
Histopathological Study ◽  
Histopathological Changes ◽  
Periodic Acid Schiff ◽  
Male Adult ◽  
Body Weight Reduction

Cyclosporine is considered one of the common worldwide immunosuppressive drugs that are used for allograft rejection prevention. However, articles that address adverse effects of cyclosporine use on the vital organs such as lung are still few. This study aims to investigate pulmonary toxic effect of cyclosporine in rats by assessment of pulmonary histopathological changes using light and electron microscope examination. Sixty male adult albino rats were divided into three groups; each group consists of twenty rats. The first received physiological saline while the second and third groups received 25 and 40 mg/kg/day of cyclosporine, respectively, by gastric gavage for forty-five days. Cyclosporine reduced the lung and body weight with shrinkage or pyknotic nucleus of pneumocyte type II, degeneration of alveoli and interalveolar septum beside microvilli on the alveolar surface, emphysema, inflammatory cellular infiltration, pulmonary blood vessels congestion, and increase of fibrous tissues in the interstitial tissues and around alveoli with negative Periodic Acid-Schiff staining. Prolonged use of cyclosporine induced pulmonary ultrastructural and histopathological changes with the lung and body weight reduction depending on its dose.

Pharmacological and Pathological Studies on Taiwan Folk Medicine (IX): The Hepatoprotective Effect of the Methanolic Extract from Echinops Grijisii

1993 ◽  
Vol 21 (01) ◽  
pp. 33-44 ◽  
Author(s):  
Chun-Ching Lin ◽  
Cheng-Hung Lin
Keyword(s):  
Liver Biopsy ◽  
Ethyl Acetate ◽  
Liver Damage ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Folk Medicine ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
Histopathological Changes ◽  
Wistar Albino Rats

In order to isolate the main hepatoprotective component of Echinops grijisii, the crude drug was extracted with methanol and subjected to continuous extractions using n-hexane chloroform, ethyl acetate and n-butanol. The hepatoprotective studies of each fraction from the methanol extract of E. grijisii was conducted in Wistar albino rats with CC14-induced liver damage. Hepatoprotective activity was evaluated in terms of the modification of serum transaminase values such as SGOT and SGPT, and histopathological changes of liver biopsy. The results indicated that the main hepatoprotective component was concentrated in n-butanol and aqueous fractions.

scholarly journals STANDARDIZATION OF MODEL OF INDUCTION OF HEPATOTOXICITY WITH ANTI-TUBERCULOSIS DRUGS IN WISTAR ALBINO RATS

2017 ◽  
Vol 10 (6) ◽  
pp. 150
Author(s):  
Sarita M Kapgate ◽  
Abhijit B Patil
Keyword(s):  
Liver Injury ◽  
Animal Studies ◽  
Hepatic Injury ◽  
Albino Rats ◽  
Histopathological Changes ◽  
First Line ◽  
Drug Induced ◽  
Significant Development ◽  
Reported Study ◽  
Wistar Albino Rats

Objective: The objective of the study to standardize the model of hepatotoxicity induced by ATT drugs in Wistar Albino rats. Isoniazid (INH), rifampicin (RMP), pyrazinamide (PZA), the first line drugs used in the treatment of tuberculosis (TB) associated with the potential adverse effect. Numerous animal studies were reported endeavoring induction and cure of anti-TB (ATT) drug-induced hepatotoxicity using herbal and chemical drugs. However, the previous reported study failed to replicate where Wistar albino rats were treated with INH, RMP, and PZA and had shown the significant development of liver injury. Hence in present paper, aimed to develop a standardize model of induction of hepatotoxicity with ATT drugs.Methods: Wistar rats were treated with ATT drugs in combination in various doses up to 4-8 weeks. Total nine experiments were conducted to achieve successful hepatotoxicity. The aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) were the biochemical parameters of assessment. Histopathological changes in the liver were also examined.Results: No evidence of any liver injury or an inflammatory infiltrate has been observed as had been reported in the previous studies. Rather decrease in serum ALT levels has been observed by researcher. In short, hepatic injury cannot be developed with the doses used in previous reported papers. The successful attempt to induce hepatotoxicity can be achieved with the doses of INH - 100, RMP - 300, PZA - 700 mg/kg. The findings were confirmed by the raised ALT, AST, and ALP levels compared with baseline. The histopathological changes also support the findings.Conclusion: The dose of INH - 100, RMP – 300 and PZM - 700 mg/kg. Succeeds to induce hepatotoxicity in Wistar albino rats and Swiss albino mice as well.

scholarly journals Effect of Newly Synthesized Polypropylene/Silver Nonwoven Fabric Dressing on Incisional Wound Healing in Rats

2014 ◽  
Vol 2 (3) ◽  
pp. 395-403 ◽  
Author(s):  
Samiha M. Gawish ◽  
Amina A. Gamal El Din ◽  
Hanaa H. Ahmed ◽  
Abdel Razik H. Farrag ◽  
Amira Abou-El Kheir
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Nonwoven Fabric ◽  
Complete Healing ◽  
Albino Rats ◽  
Tissue Formation ◽  
Histopathological Changes ◽  
Granulation Tissue Formation ◽  
Wound Length ◽  
Incision Wound

AIM: The present work aimed to investigate the effect of the antimicrobial nano Ag/polypropylene (PP\Ag) dressing on incisional wound healing on the experimental level.MATERIALS AND METHODS: Male albino rats were divided into, control, PP/Ag dressing, Silver Sulfadiazine (Ag-SD) cream, blank PP dressing and undressed groups. Animals were sacrificed after 5, 10 and 15 days of incisional wound event.RESULTS: Reduction was found in incision wound length in PP/Ag dressed rats, Ag-SD cream treated rats, and blank PP dressed rats after 5, 10 and 15 days compared to undressed rats. Skin of PP/Ag group showed less adverse histopathological changes, enhanced granulation tissue formation, enhanced angiogenesis, accelerated re-epithelialization and quick complete healing; compared to all other groups. Significant decrease in TGF-β level was recorded in PP\Ag and Ag-SD cream groups as compared to blank PP group on day 5. While, significant decrease in TGF-β level was detected in PP\Ag group when compared with undressed and blank PP groups on day 10. TGF-β showed significant in PP\Ag group as compared to undressed, Ag-SD cream and blank PP groups on day 15. CONCLUSION: The present results suggest that PP/Ag dressing enhances, promotes and plays an important role in wound healing.

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