SOME STUDIES ON PASTEURELLA MULTOCIDA AS A CAUSATIVE AGENT OF MASTITIS IN DAIRY COWS AND EWES

Cattle are primary reservoirs of Escherichia coli O157:H7, a causative agent of severe human infections. To facilitate analyses of the communities in which this pathogen is found, we sequenced the fecal metagenomes of 10 dairy cows shedding E. coli O157:H7 and added them to the public domain.

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Mass spectrometric analysis of lipid A obtained from the lipopolysaccharide of Pasteurella multocida

RSC Advances ◽

10.1039/d0ra05463a ◽

2020 ◽

Vol 10 (51) ◽

pp. 30917-30933

Author(s):

Abdul Tawab ◽

Noor Akbar ◽

Mujtaba Hasssan ◽

Fazale Habib ◽

Aamir Ali ◽

...

Keyword(s):

Causative Agent ◽

Pasteurella Multocida ◽

Lipid A ◽

Water Buffalo ◽

Mass Spectrometric ◽

Mass Spectrometric Analysis ◽

Spectrometric Analysis ◽

Type B ◽

Haemorrhagic Septicaemia

LC/MS-based variant profiling of lipid A component of endotoxic lipopolysaccharides of Pasteurella multocida type B:2, a causative agent of haemorrhagic septicaemia in water buffalo and cattle.

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Signature-Tagged Mutagenesis of Pasteurella multocida Identifies Mutants Displaying Differential Virulence Characteristics in Mice and Chickens

Infection and Immunity ◽

10.1128/iai.71.9.5440-5446.2003 ◽

2003 ◽

Vol 71 (9) ◽

pp. 5440-5446 ◽

Cited By ~ 44

Author(s):

Marina Harper ◽

John D. Boyce ◽

Ian W. Wilkie ◽

Ben Adler

Keyword(s):

Animal Models ◽

Virulence Factors ◽

Causative Agent ◽

Pasteurella Multocida ◽

Disease Model ◽

Competitive Growth ◽

Fowl Cholera ◽

First Time ◽

Chicken Model ◽

Specific Virulence

ABSTRACT Pasteurella multocida is the causative agent of fowl cholera in birds. Signature-tagged mutagenesis (STM) was used to identify potential virulence factors in a mouse septicemia disease model and a chicken fowl cholera model. A library of P. multocida mutants was constructed with a modified Tn916 and screened for attenuation in both animal models. Mutants identified by the STM screening were confirmed as attenuated by competitive growth assays in both chickens and mice. Of the 15 mutants identified in the chicken model, only 5 were also attenuated in mice, showing for the first time the presence of host-specific virulence factors and indicating the importance of screening for attenuation in the natural host.

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A Heptosyltransferase Mutant of Pasteurella multocida Produces a Truncated Lipopolysaccharide Structure and Is Attenuated in Virulence

Infection and Immunity ◽

10.1128/iai.72.6.3436-3443.2004 ◽

2004 ◽

Vol 72 (6) ◽

pp. 3436-3443 ◽

Cited By ~ 54

Author(s):

Marina Harper ◽

Andrew D. Cox ◽

Frank St. Michael ◽

Ian W. Wilkie ◽

John D. Boyce ◽

...

Keyword(s):

Mass Spectrometry ◽

Nuclear Magnetic Resonance ◽

Sequence Analysis ◽

Magnetic Resonance ◽

Causative Agent ◽

Pasteurella Multocida ◽

Full Length ◽

Wild Type ◽

Fowl Cholera ◽

Insertional Inactivation

ABSTRACT Pasteurella multocida is the causative agent of fowl cholera in birds. In a previous study using signature-tagged mutagenesis, we identified a mutant, AL251, which was attenuated for virulence in mice and in the natural chicken host. Sequence analysis indicated that AL251 had an insertional inactivation of the gene waaQPM , encoding a putative heptosyl transferase, required for the addition of heptose to lipopolysaccharide (LPS) (M. Harper, J. D. Boyce, I. W. Wilkie, and B. Adler, Infect. Immun. 71:5440-5446, 2003). In the present study, using mass spectrometry and nuclear magnetic resonance, we have confirmed the identity of the enzyme encoded by waaQPM as a heptosyl transferase III and demonstrated that the predominant LPS glycoforms isolated from this mutant are severely truncated. Complementation experiments demonstrated that providing a functional waaQPM gene in trans can restore both the LPS to its full length and growth in mice to wild-type levels. Furthermore, we have shown that mutant AL251 is unable to cause fowl cholera in chickens and that the attenuation observed is not due to increased serum sensitivity.

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Isolation and identification of the causative agent of fowl cholera in Iraq and preparation of vaccines against the disease 1- Isolation of the causative agent of fowl cholera and study of it’s properties.

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v29i1.859 ◽

2005 ◽

Vol 29 (1) ◽

pp. 11-21

Author(s):

Hatem M . Mhanam

Keyword(s):

Causative Agent ◽

Pasteurella Multocida ◽

Isolation And Identification ◽

Antibacterial Drugs ◽

Fowl Cholera

It was possible to obtain 17 isolates of Pasteurella multocida frominfected laying chickens with fowl cholera. Three isolates were from the chronicphases of the naturally infected chickens and other fourteen were from the acutephases. The identification of these isolates was considered. Fifteen isolatesbelong to the sub species multocida and two isolates to gallicida, ten isolatesbelong to the SE-190 isolate and one to SE-077, while six isolates were notidentified. Twelve of the isolates have a capsule. The LD50s of these isolateswere varied. The results of sensitivity to antibacterial drugs revieled that allisolates were sensitive to Penicillin and resistance to Lincomycin with variousdegrees of sensitivity to other antibacterial drugs.

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Pasteurella multocida mastitis in cow - case report

Veterinarski glasnik ◽

10.2298/vetgl170619011m ◽

2017 ◽

Vol 71 (2) ◽

pp. 117-122

Author(s):

Dubravka Milanov ◽

Nevenka Aleksic ◽

Dalibor Todorovic ◽

Dejan Bugarski

Keyword(s):

Dairy Cows ◽

Pasteurella Multocida ◽

Bird Species ◽

Domestic Animals ◽

Clinical Findings ◽

Laboratory Diagnostics ◽

Gram Negative Bacteria ◽

Past Medical History ◽

Source Of Infection ◽

Upper Respiratory

Pasteurella (P.) multocida is a heterogeneous species of Gram-negative bacteria which are common commensals of the upper respiratory system of various mammal and bird species, but are also opportunistic contagious zoonotic pathogens which cause a wide spectre of infections in domestic animals and humans. P. multocida is a rare cause of mastitis in dairy cows. The source of infection mainly remains unknown, mastitis usually is acute, and the therapy by intramammary administration of antibiotics does not lead to satisfactory results. Lethality is possible due to presence of endotoxins in blood. Literature data on P. multocida mastitis in dairy cows is particularly scarce, which is why such a case is described in the current work, with past medical history, clinical findings, laboratory diagnostics and therapeutic approach.

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Serological types of Pasteurella multocida isolated from turkeys and chickens in Canada

Canadian Journal of Microbiology ◽

10.1139/m82-160 ◽

1982 ◽

Vol 28 (9) ◽

pp. 1078-1080 ◽

Cited By ~ 2

Author(s):

J. L. Bhasin

Keyword(s):

Causative Agent ◽

Pasteurella Multocida ◽

Antigenic Determinants ◽

Poultry Industry ◽

Fowl Cholera ◽

Precipitation Patterns ◽

Precipitin Test ◽

Type 3 ◽

Diffusion Precipitation ◽

Gel Diffusion

Outbreaks of fowl cholera continue to plague the Canadian poultry industry despite widespread immunization against the causative agent, Pasteurella multocida. Fowl cholera bacterins currently employed by domestic poultry growers contain three serological types, namely, serotypes 1, 3, and 4. In this study a total of 84 strains of P. multocida were isolated in Canada from outbreaks of fowl cholera in turkeys and chickens. Serotyping was accomplished using the gel diffusion precipitin test. Based on the gel diffusion precipitation patterns, 27 serotypes containing one to six antigenic determinants were recognized. The most prevalent serotype both in turkeys and chickens appeared to be type 3. Significantly, greater than 20% of P. multocida isolates failed to react with antisera raised against serotypes 1, 3, and 4.

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Validation of a Novel ELISA for the Diagnosis of Hemorrhagic Septicemia in Dairy Cattle from Thailand Using a Bayesian Approach

Veterinary Sciences ◽

10.3390/vetsci7040163 ◽

2020 ◽

Vol 7 (4) ◽

pp. 163

Author(s):

Tawatchai Singhla ◽

Pallop Tankaew ◽

Nattawooti Sthitmatee

Keyword(s):

Immune Responses ◽

Dairy Cows ◽

Bayesian Approach ◽

Posterior Probability ◽

Pasteurella Multocida ◽

Elisa Test ◽

Heat Extraction ◽

Enzyme Linked Immunosorbent Assay ◽

Probability Interval ◽

Indirect Hemagglutination

The objective of this study was to estimate sensitivity (Se) and specificity (Sp) of a novel enzyme-linked immunosorbent assay (ELISA) test (using a coating antigen from Pasteurella multocida M-1404 via heat extraction) and an indirect hemagglutination (IHA) test for detection of Hemorrhagic septicemia (HS) in dairy cows, under Thai conditions, using a Bayesian approach. Dairy cow sera with a total of 1236 samples from 44 farms were tested with the two tests to detect immune responses against the HS. Percentages of positive samples for the ELISA and IHA tests were 73% (901/1236) and 70% (860/1236), respectively. Estimated sensitivity and estimated specificity of the ELISA test were 90.5% (95% posterior probability interval (PPI) = 83.2–95.4%) and 70.8% (95% PPI = 60.8–79.8%), respectively. Additionally, estimates for the Se and Sp values of the IHA test were 77.0% (95% PPI = 70.8–84.1%) and 51.1% (PPI = 36.8–66.3%), respectively. The estimated prevalence of the disease was 71.7% (95% PPI = 62.7–82.6%). These results demonstrate that the ELISA test can be a useful tool for the detection of the presence of an antibody against the HS in dairy cows. Notably, the cows in this area indicated a high percentage of exposure to Pasteurella multocida.

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Mycoplasma wenyonii: A Causative Agent of New Mastitis in Dairy Cows

Advances in Animal and Veterinary Sciences ◽

10.17582/journal.aavs/2019/7.6.480.483 ◽

2019 ◽

Vol 7 (6) ◽

Author(s):

Salam Abd-Esmaeel ◽

Basima Abdulfatah Albadrani

Keyword(s):

Dairy Cows ◽

Causative Agent ◽

Mycoplasma Wenyonii

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Antimicrobial activity of enrofloxacin in vitro

International bulletin of Veterinary Medicine ◽

10.17238/issn2072-2419.2020.2.99 ◽

2020 ◽

Vol 2 ◽

pp. 99-103

Author(s):

D.V. Yurin ◽

◽

V. V. Nevzorova ◽

A.A. Balbutskaya ◽

S.S. Belimova ◽

...

Keyword(s):

Causative Agent ◽

Pathogenic Bacteria ◽

Pasteurella Multocida ◽

Coagulase Negative Staphylococci ◽

Salmonella Dublin ◽

Streptococcus Uberis ◽

Swine Erysipelas ◽

Diffusion Assay ◽

Corre Spondence

Continuouse use of enrofloxacin contributes to emergence of enrofloxacin-resistant mi-crobial resistance, isolated and reported late-ly. In this study we deal with the spread of resistance of enrofloxacin among pathogenic organisms, infecting animals. The suscepti-bility to enrofloxacin was studied in standard disc diffusion assay. We studied 437 bacteri-al isolates in total. Salmonella dublin and Sal-monella typhimurium showed the highest suscepti-bility to enrofloxacin (100%); Salmonella enter-itidis and Salmonella choleraesuis proved a bit less susceptibility (95% and 94,7%). 5% of S. enter-itidis isolates and 5.3% of S. choleraesuis isolates had intermediate susceptibility. We did not register any resistance of isolates of Salmonella, Pasteurella and Morganella (Pasteurella multocida, Morganel-la morganii). 83.9% of Escherichiacoli strains proved susceptibility to enrofloxacin, the zone of retardation in 6.4% of the isolates was in corre-spondence with intermediate susceptibility, 9.7% of the isolates proved to be resistant. 90,9% of Pseudomonas aeruginosa isolates in our study was susceptible to enrofloxacin, 9.1% of them had intermediate susceptibility. The isolates of Strepto-coccus spp. and Staphylococcus pseudintermedius re-vealed high susceptibility to enrofloxacin, also as Listeria monocytogenes (causative agent of listeriosis)and Erysipe-lothrix rhusiopathiae (causative agent of swine erysipelas). 87.5% of the coagulase negative staphylococci proved susceptible to enrofloxacin; 6.25% of the isolates were resistant or had intermediate susceptibility. The shares of susceptible isolates of Staphylococcus hyicus, Staphylococ-cus aureus and Streptococcus uberis were respec-tively 65.1%, 75%, 75%. The shares of isolates with intermediate susceptibility of the same spp. were respectively 9.3%, 15%, 25%. The shares of resistant isolates of Staphylococci were respective-ly 25.6% and 10%. We found no strains of Str. uberis with resistance to enrofloxacin. As for Enterococci, 52.4% of the isolates were enrofloxacin-susceptible, 11,9% and 37,7% of them were re-spectively enrofloxacin-resistant or had intermedi-ate susceptibility. Presently most Gram-negative pathogenic bacteria have no resistance to enroflox-acin. Notwithstanding that enrofloxacin is signifi-cantly less effective against such pathogenic organ-isms as Staphylococci and Streptococci.

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