Development of Okra (Abelmoschus esculentus L. Moench) Hybrids Derived from Selected Inbreds under Drought Stress

As climate changes increase, drought stress is becoming a problem for all major horticultural crops; among them is okra (Abelmoschus esculentus). Despite its superior resilience to heat stress and high nutritional content, it is still underutilized in contrast to other vegetable crops. Moreover, the drought-resistant and drought-sensitive genotypes of okra are also not well known and require further exploration to improve their productivity. To investigate this in more detail, we performed comparative physiological and large-scale chloroplast proteomics on drought-stressed genotypes of okra. We evaluated four major genotypes of okra, viz., NS7774, NS7772, Green Gold, and OH3312 for drought resilient rootstock. The physiological modulations demonstrated a significant change by 50–76% in biomass, net-photosynthetic machinery, water transport, and absorption both in early and late stages of drought stress compared to well-watered crops in all genotypes. Maximum oxidative damage due to drought stress was observed for the genotypes NS7772, Green Gold and OH3312 as depicted by H2O2 and O2− determination. Greater oxidative stress was correlated to lesser antioxidant activity and expression of antioxidant enzymes, such as catalase and ascorbate peroxidase under stress in okra genotypes. The overall photosynthetic pigments, such as total chlorophyll, and total carotenoid content, were also decreased, and stomatal guard cells were disrupted and appeared closed compared to the control for the above three mentioned genotypes, except NS7774. A subsequent tissue-specific proteome analysis of chloroplasts and thylakoids analyzed by BN-PAGE (blue native polyacrylamide gel electrophoresis) revealed either over or under expression of specific proteins, such as ATPase, PSI, PSII core dimer, PSII monomer and ATP synthase. The expression of multiprotein complex proteins, including PSII-core dimer and PSII-core monomer, was slightly higher for the genotype NS7774 when compared to three other genotypes for both 5 and 10 days of drought stress. Further identification of specific proteins obtained in second dimension BN-PAGE provided descriptive detail of seven proteins involved in drought resistance across all genotypes. The identified proteins are majorly involved in photosynthesis under drought stress, suggesting NS7774 as a drought tolerant genotype. Further, the proteomic results were confirmed using Immunoblot by selecting specific protein such as PsaA. Overall, from our physiological modulations and chloroplast proteomics in all genotypes, we summarized NS7774 as a resilient rootstock and the other three genotypes (NS7772, OH3312, and Green Gold) as sensitive ones.

Download Full-text

Paclobutrazol mediated changes in growth and physio-biochemical traits of okra (Abelmoschus esculentus L.) grown under drought stress

Gene Reports ◽

10.1016/j.genrep.2020.100908 ◽

2020 ◽

Vol 21 ◽

pp. 100908

Author(s):

Shazia Iqbal ◽

Nusrat Parveen ◽

Saraj Bahadur ◽

Tanveer Ahmad ◽

Muhammad Shuaib ◽

...

Keyword(s):

Drought Stress ◽

Abelmoschus Esculentus ◽

Biochemical Traits

Download Full-text

hk THE EFFECT OF DROUGHT STRESS ON THE GROWTH AND YIELD OF SEVERAL OKRA GENOTYPES (Abelmoschus esculentus L.)

CROP AGRO Scientific Journal of Agronomy ◽

10.29303/caj.v12i01.253 ◽

2019 ◽

Vol 12 (01) ◽

pp. 29

Author(s):

Husnul Khotimah

Keyword(s):

Drought Stress ◽

Plant Height ◽

Dry Weight ◽

Abelmoschus Esculentus ◽

Growth And Yield ◽

Treatment Combination ◽

Main Plot ◽

Wet Weight ◽

Almost All ◽

Plot Design

ABSTRACT Okra (Abelmoschus esculentus L.) is one of horticulture crop that contain high nutrition. Growth and yield of this crop determined by genetic and environment factor especially water. The objective of this research was to know the effect of drought stress toward growth and yield of several okra genetypes. This research was undertaken in plastic house, Batu Kuta village Sub regency of Narmada using Split Plot design with drought stress as main plot and genotype as sub plot. Main plot consist of drought stress and without drought stress. Sub plot consist of Dompu genotype, Hybrid genotype, Red genotype and Long genotype. Every treatment combination was replicated three times. Result of this study showed that interaction between treatment and genotype of okra effected on canopy wet weight, canopy dry weight, plant height at 60 days after planting, number of leaf at 30, 60 and 90 days after planting, number of flower becoming fruit and number of good fruit. Treatment of okra genotype effected on almost all parameters except wet weight of root, dry weight of root and diameter of stem 30 days after planting. While, treatment of drought stress only effected on canopy wet weight and plant height at 60 and 90 days after planting. Long genotype had the best growth on the condition of drought. Red genotype had the best number of good fruit on the condition of drought stress and without drought stress.

Download Full-text

Flavonoid profiling by LC-MS IN 14 – 3-3ν knockout mutants of Arabidopsis thaliana during drought stress conditions

Planta Medica ◽

10.1055/s-0034-1382678 ◽

2014 ◽

Vol 80 (10) ◽

Cited By ~ 1

Author(s):

F Nabbie ◽

O Shperdheja ◽

J Millot ◽

J Lindberg ◽

B Peethambaran

Keyword(s):

Arabidopsis Thaliana ◽

Drought Stress ◽

Stress Conditions ◽

Knockout Mutants

Download Full-text

Mitigation Effects of 24-Epibrassinolide and Thiourea in Field Pea (Pisum sativum L.) under Drought Stress

THE JOURNAL OF PLANT SCIENCE RESEARCH ◽

10.32381/jpsr.2018.34.02.11 ◽

2018 ◽

Vol 34 (2) ◽

pp. 229-235 ◽

Cited By ~ 1

Author(s):

Prachi Garg ◽

◽

A. Hemantaranjan ◽

Jyostnarani Pradhan ◽

◽

...

Keyword(s):

Drought Stress ◽

Pisum Sativum ◽

Field Pea ◽

Pisum Sativum L

Download Full-text

Growth and yield of okra (Abelmoschus esculentus (L.) Moench) grown in the marginal upland area of Sta. Rita, Samar as influenced by different planting densities and mulching materials

Annals of Tropical Research ◽

10.32945/atr39sa4.2017 ◽

2017 ◽

pp. 44-54

Author(s):

Zenaida Gonzaga ◽

Warren Obeda ◽

Ana Linda Gorme ◽

Jessie Rom ◽

Oscar Abrantes ◽

...

Keyword(s):

Bare Soil ◽

Abelmoschus Esculentus ◽

Growth And Yield ◽

Planting Density ◽

Rice Hull ◽

Plastic Mulch ◽

Lady’S Finger ◽

Materials Used ◽

Set Up ◽

A Minor

Okra or Lady’s finger, botanically known as Abelmoschus esculentus (L.) Moench, is a tropical and sub-tropical indigenous vegetable crop commonly grown for its fibrous, slimy, and nutritious fruits and consumed by all classes of population. It has also several medicinal and economic values. Despite its many uses and potential value, its importance is under estimated, under-utilized, and considered a minor crop and little attention was paid to its improvement. The study was conducted to evaluate the effects of different planting densities and mulching materials on the growth and yield of okra grown in slightly sloping area in the marginal uplands in Sta. Rita, Samar, Philippines. A split-plot experiment was set up with planting density as main plot and the different mulching materials as the sub-plot which were: unmulched or bare soil, rice straw, rice hull, hagonoy and plastic mulch. Planting density did not significantly affect the growth and yield of okra. Regardless ofthe mulching materials used, mulched plants were taller and yielded higher compared to unmulched plants. Moreover, the use of plastic mulch resulted to the highest total fruit yield. The results indicate the potential of mulching in increasing yield and thus profitability of okra production under marginal upland conditions.

Download Full-text