scholarly journals Features of oral immunity indicators in patients with phlegmons of the maxillofacial region against the background of insulin-independent diabetes mellitus

2021 ◽  
Vol 24 (1) ◽  
pp. 10-18
Author(s):  
Andrei V. Shchenin
Keyword(s):  
Diabetes Mellitus ◽  
Interleukin 6 ◽  
Maxillofacial Surgery ◽  
Interleukin 10 ◽  
Hospital Treatment ◽  
Control Group ◽  
Control Groups ◽  
Maxillofacial Region ◽  
Mar Index ◽  
And Control

Objective: studying the interrelations of the local oral immunity indicators which allow to predict the severity of phlegmons course in patients with maxillofacial region phlegmons against the background of insulin-independent diabetes mellitus. Materials and methods. 44 patients were included in this prospective study. The main group: 19 patients with the maxillofacial region phlegmons and the type 2 diabetes mellitus as a concomitant pathology (PH+DM). The comparison group: 20 patients with the maxillofacial region phlegmons with no diabetes mellitus among the concomitant pathologies (PH). The control group: 5 patients were undergoing routine surgical treatment in the maxillofacial surgery department. The groups were comparable in age, gender, and phlegmon treatment (p > 0.05). Levels of the proinflammatory and anti-inflammatory cytokines, the humoral immunity factors and the microbial adsorption reaction (MAR) index to the oral epithelial cells were determined in the saliva of patients on the 1st and 5th days. The obtained data were statistically processed. Results. On the 1st day of hospital treatment the PH+DM group revealed an increase in interleukin-6, a decrease in interleukin-10 and lactoferrin as compared to the PH and control groups (p < 0.05). On the 5th day of the treatment the interleukin-6 and tumor necrosis factor-α levels did not differ between PH+DM and the control groups (p < 0.05). The interleukin-4 and interleukin-10 levels were lower in the PH+DM and PH groups than in the control group on the 5th day (p < 0.05). The lactoferrin level in the PH+DM group was lower than in the PH and control groups (p < 0.05). The significant negative correlation was found between the MAR index values and SOFA (Sequential organ failure assessment score) points in patients with sepsis, R = -0.9 (p < 0.05). Conclusion. The delayed regression of the saliva inflammation indicators was specific for the PH+DM group patients in comparison with the PH group patients. The MAR index below 10% may be a predictor of unfavorable course of the phlegmon development.

scholarly journals Relationship between TP53 and interleukin-6 gene variants and the risk of types 1 and 2 diabetes mellitus development in the Kermanshah province

2021 ◽  
Vol 14 (1) ◽  
pp. 37-44
Author(s):  
Lida Haghnazari ◽  
◽  
Ramin Sabzi ◽  
◽  
Keyword(s):  
Diabetes Mellitus ◽  
Tp53 Gene ◽  
Control Group ◽  
Control Groups ◽  
Codon 72 ◽  
Type 2 Dm ◽  
And Control ◽  
Type 1 Dm

Diabetes mellitus (DM) is a metabolic disorder that results from insufficient secretion or insulin resistance, or both. Insulin secretion deficiency leads to chronic hyperglycemia along with impaired metabolism of proteins, lipids, and carbohydrates. This study aimed to investigate the TP53 gene SNP (single nucleotide polymorphism) rs1042522 genotype and the interleukin-6 (IL-6) gene SNP rs1800795 genotype in DM and control groups. This study was performed on 70 patients with type 1 DM, 100 patients with type 2 DM without related complications, 66 control subjects for type 1 DM, and 95 control subjects for type 2 DM. The control groups were matched regarding age and gender and did not have a familial relationship with the patient groups. All the subjects were residents of Kermanshah, located in the western part of Iran. Polymorphisms of TP53 and IL-6 genes were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Lipid profile, fasting blood glucose, and HbA1c were measured using the ELISA and immunoturbidometric methods. The frequency of genotypes (CC, CG, GG) of the TP53 gene codon 72 in type 1 DM and its control group were significantly different (P= 0.013). Likewise, the frequency of genotypes (CC, CG, GG) of the TP53 gene codon 72 was significantly different between type 2 DM and control groups (P <0.001). The frequency of genotypes (GG, GC, CC) of G174C polymorphisms in the IL-6 gene was different between type 1 DM and control group as well as between type 2 DM and its control group, but it was not statistically significant. SNP rs1042522 genotypes in the dominant form (CG + GG vs. CC) (OR= 3.880; P < 0.001) and alleles G vs. C alleles (OR= 0.384; P < 0.001) increased the risk of type 2 DM significantly. There was no significant difference between type 1 and type 2 DM groups and respected control groups regarding the frequency of the IL-6 gene SNP rs1800795 alleles. The G allele of SNP rs1042522 encoding the TP53 gene increases the risk of developing DM in the population of the Kermanshah province, Iran.

scholarly journals Bioinformatic Analysis of Evolutional Conservatism and Functional Significance of Microsatellite Alleles of Human 14Q13.2 Region Associated with Type 2 Diabetes Mellitus

2008 ◽  
Vol 62 (3) ◽  
pp. 91-102 ◽  
Author(s):  
Tatjana Sjakste ◽  
Ilva Poudžiunas ◽  
Valdis Pīrāgs ◽  
Māris Lazdiñš ◽  
Nikolajs Sjakste
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Functional Significance ◽  
Bioinformatic Analysis ◽  
Control Group ◽  
Case Group ◽  
Control Groups ◽  
And Control ◽  
Microsatellite Alleles

Bioinformatic Analysis of Evolutional Conservatism and Functional Significance of Microsatellite Alleles of Human 14Q13.2 Region Associated with Type 2 Diabetes Mellitus The paper deals with bioinformatic and statistical analysis of the possible functional significance of the previously shown association of several microsatellite alleles in intron 6 of the human proteasome core particle PSMA6 gene (HSMS006) and four other microsatellites localised upstream in human chromosome 14q13.2 (HSMS801, HSMS702, HSMS701, HSMS602) with type 2 diabetes mellitus in Latvia and Botnia, Finland. Genotype analysis revealed that (CAA)8/(CAA)8 homozygotes of the HSMS602 marker were never found in Type 2 diabetes patients, although 6.56% of the individuals from the control groups were the (CAA)8/(CAA)8 homozygotes. For the HSMS801 marker the (AC)21/(AC)23 genotype was never found in the case group and in the control group it was detected with a frequency 4.40%; these differences were statistically significant (P < 0.05). In contrast to the Latvian population, the distribution of genotype frequencies in cases and controls taken from the Botnian dataset was almost similar. Haplotype analysis showed that in the Latvian population besides haplotypes including alleles differently represented in case and control groups, a combination of some alleles almost equally represented in both groups formed combinations that were more characteristic of either the case group or the control group. This indicates probable independent functional significance of these haplotypes that warrants further investigation. In the Botnian population, more allele combinations were observed, and the distribution of haplotypes in case and control groups differed from that observed in Latvia. The observed haplotype distributions might reflect differences between the studied populations: a homogenous and isolated Botnian vis-à-vis a mixed Latvian population. Linkage disequilibrium (LD) analysis of data on the Latvian population revealed nine of ten two-allele combinations manifesting a high LD. HSMS006 and HSMS602 combination had a low LD; among the analysed markers these were situated at the largest distance from one another. Data on the Botnian population showed that haplotypes in eight of ten combinations had a high LD, including the HSMS006 and HSMS602 combinations. It appears that the two populations differ also in linkage disequilibrium of two-loci haplotypes. Theoretical analysis of a potential functional role of the polymorphisms indicated the significance of the microsatellite length of HSMS602 and HSMS006 for the formation of DNA hairpins. The whole genomic region appears to be conservative in mammals.

scholarly journals Drift of the Subgingival Periodontal Microbiome during Chronic Periodontitis in Type 2 Diabetes Mellitus Patients

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 504
Author(s):  
Irina P. Balmasova ◽  
Evgenii I. Olekhnovich ◽  
Ksenia M. Klimina ◽  
Anna A. Korenkova ◽  
Maria T. Vakhitova ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Relative Abundance ◽  
Chronic Periodontitis ◽  
Control Group ◽  
Control Groups ◽  
And Control ◽  
Pooled Samples

Since periodontitis and type 2 diabetes mellitus are complex diseases, a thorough understanding of their pathogenesis requires knowing the relationship of these pathologies with other disorders and environmental factors. In this study, the representability of the subgingival periodontal microbiome of 46 subjects was studied by 16S rRNA gene sequencing and shotgun sequencing of pooled samples. We examined 15 patients with chronic periodontitis (CP), 15 patients with chronic periodontitis associated with type 2 diabetes mellitus (CPT2DM), and 16 healthy subjects (Control). The severity of generalized chronic periodontitis in both periodontitis groups of patients (CP and CPT2DM) was moderate (stage II). The male to female ratios were approximately equal in each group (22 males and 24 females); the average age of the subjects was 53.9 ± 7.3 and 54.3 ± 7.2 years, respectively. The presence of overweight patients (Body Mass Index (BMI) 30–34.9 kg/m2) and patients with class 1–2 obesity (BMI 35–45.9 kg/m2) was significantly higher in the CPT2DM group than in patients having only chronic periodontitis or in the Control group. However, there was no statistically significant difference in all clinical indices between the CP and CPT2DM groups. An analysis of the metagenomic data revealed that the alpha diversity in the CPT2DM group was increased compared to that in the CP and Control groups. The microbiome biomarkers associated with experimental groups were evaluated. In both groups of patients with periodontitis, the relative abundance of Porphyromonadaceae was increased compared to that in the Control group. The CPT2DM group was characterized by a lower relative abundance of Streptococcaceae/Pasteurellaceae and a higher abundance of Leptotrichiaceae compared to those in the CP and Control groups. Furthermore, the CP and CPT2DM groups differed in terms of the relative abundance of Veillonellaceae (which was decreased in the CPT2DM group compared to CP) and Neisseriaceae (which was increased in the CPT2DM group compared to CP). In addition, differences in bacterial content were identified by a combination of shotgun sequencing of pooled samples and genome-resolved metagenomics. The results indicate that there are subgingival microbiome-specific features in patients with chronic periodontitis associated with type 2 diabetes mellitus.

scholarly journals STUDY OF MICROSCOPIC IMPRINTS OF PURULENT WOUNDS OF THE MAXILLOFACIAL REGION IN CHILDREN WHEN USING THE OINTMENT WITH FURACILIN, LIDOCAINE AND DIBUNOLUM

2019 ◽  
Vol 15 (2) ◽  
pp. 97-101
Author(s):  
Сергей Чуйкин ◽  
Sergey Chuykin ◽  
Тимур Хасанов ◽  
Timur KXASANOV ◽  
Тагир Мустафин ◽  
...  
Keyword(s):  
Bacterial Contamination ◽  
Inflammatory Diseases ◽  
Main Group ◽  
Control Group ◽  
Wound Surface ◽  
Control Groups ◽  
Maxillofacial Region ◽  
Morphological Studies ◽  
Purulent Wounds ◽  
And Control

Importance ― study of microscopic imprints of the wound surface of purulent wounds in inflammatory diseases of the maxillofacial region in children. Objectives ― cytological research of microscopic imprints of purulent wounds in inflammatory diseases of the maxillofacial region in children Methods. The study included 60 children from 5 to 16 years old who were treated for abscesses and phlegmon of the maxillofacial area. Patients were divided independently into 2 groups. The main group included 30 children. For their treatment we used ointment with Furacilin, Lidocaine and Dibunolum. The control group included 30 patients. For their treatment used ointment Levomecol. To compare the effectiveness of the healing of purulent wounds in the main and control groups, we studied cytology of microscopic imprints of purulent wounds at 3, 7 and 10 days. Results. A microscopic examination of the imprints of the wound surface of the control group on the 3 day revealed a predominantly inflammatory type of cytogram, on day 5, the type of cytorgamy was replaced by inflammatory-regenerative, which persisted on the 7th day of the study. Microflora was represented in small quantities. In the main group, in the study of microscopic imprints on the 3ed day, inflammatory-regenerative type of cytogram was also observed. On the 7th, the cytogram shifted to the regenerative type. Cell infiltrate was poor, containing macrophages and lymphocytes. Seeding by microorganisms was not observed. Conclusions. Thus, based on the morphological studies of microscopic imprints of the wound surface, we recommend the use of ointment with Furacilin, Lidocaine and Dibunolum for the complex treatment of abscesses and phlegmon of the maxillofacial region. Since the ointment reduces the time of wound healing and reduces bacterial contamination of the wound.

scholarly journals The Effect of a Sexual Health Promotion Training Program on Sexual Function of Pregnant Women with Gestational Diabetes Mellitus

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Elahe Fayyazian ◽  
Farnoosh Khojasteh ◽  
Farshid Saeedinezhad ◽  
Maryam Haghighi
Keyword(s):  
Diabetes Mellitus ◽  
Health Promotion ◽  
Sexual Health ◽  
Sexual Function ◽  
Pregnant Women ◽  
Training Program ◽  
Intervention Group ◽  
Control Group ◽  
Control Groups ◽  
And Control

Background: Pregnancy care and screening programs are very important, especially in high-risk pregnancies. However, few interventional studies have addressed mental and sexual health in women with gestational diabetes mellitus (GDM). Objectives: The present study aimed to examine the effect of a sexual health promotion training program on sexual function of pregnant women with GDM referring to comprehensive health centers in Zahedan, Iran, in 2020. Methods: This quasi-experimental study was performed on 80 pregnant women with GDM (gestational age: 24 - 30 weeks). The participants were selected using multi-stage sampling method and randomly assigned into intervention and control groups. The participants in the intervention group received sexual health promotion training in four sessions (60 - 90 minutes) two sessions per week. The participants in the control group received routine care. The demographic information questionnaire was completed by the participants at the beginning of the study, and the Female Sexual Function Index (FSFI) was administered before and four weeks after the completion of the sexual health promotion training program. The collected data were analyzed in SPSS software (version 22) using independent samples t-test, paired samples t-test, analysis of covariance (ANCOVA), and chi-square test. Results: The results showed a statistically significant difference between the intervention and control groups in all subscales of sexual function (P < 0.05). Moreover, the results of ANCOVA for the total sexual function score showed that the women in the intervention group had significantly higher levels of sexual function (22.89 ± 3.24) compared to the women in the control group (16.78 ± 3.16) (P = 0.001). Conclusions: Given the positive and significant effect of sexual health promotion training on the sexual function of pregnant women with GDM, it is recommended to integrate this training program in prenatal care of these vulnerable women.

scholarly journals Anti-Inflammatory Markers IL-10 and IL-35: Role in Developing Gestational Diabetes Mellitus

2020 ◽  
Author(s):  
Adele Bahar ◽  
Ozra Akha ◽  
Mahdi Bordbar ◽  
Saeid Abediankenari ◽  
Rezaali Mohammadpoor ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Pregnant Women ◽  
Inflammatory Marker ◽  
Control Group ◽  
Control Groups ◽  
The Mean ◽  
Anti Inflammatory ◽  
And Control

Introduction: Inflammatory state is considered as the pathogenesis of Gestational Diabetes Mellitus (GDM). Cytokines can cause insulin resistance and maybe the molecular basis of inflammation in Diabetes Mellitus (DM). Aim: To assess the level of Interleukin-10 (IL-10) in addition to a new anti-inflammatory cytokine marker Interleukin-35 (IL-35) in pregnant women with and without GDM. Materials and Methods: Participants in the study included 29 pregnant women with GDM (case group) and 29 healthy pregnant women (control group). Blood levels of IL-10, IL-35, Erythrocyte Sedimentation Rate (ESR) and C-Reactive Protein (CRP) were measured in all participants. Independent t-test and Chi-square test were used for data analysis. Quantitative data between three gestational subgroups (<29, 29-32 and >32 weeks) in each GDM and control group were compared by ANOVA test. The p-value <0.05 was considered significant. Results: The mean levels of IL-10 were 1.03±0.85 and 0.83±0.57 pg/mL (p=0.284) and the mean IL-35 concentrations were 10.2±8.1 and 8.8±4.3 pg/mL (p=0.437) in GDM and control groups, respectively. The mean CRP and ESR levels were higher in the GDM group than the controls but the differences were not statistically significant. In the GDM group, IL-10 was significantly lower at the early stage of pregnancy (<29 weeks) compared to the later stage (>32 weeks) (p=0.04), but this was not true in the control group. There was no significant difference between the mean level of IL-35 at different gestational ages in both GDM and control groups. Conclusion: The present study showed the decreased level of anti-inflammatory marker IL-10 in the late stage of pregnancy in diabetic women especially during the last weeks of gestation. New inflammatory marker IL-35 was not statistically significant in GDM subjects.

scholarly journals EVALUATION OF RENAL FUNCTION IN SUDANESE DIABETIC AND HYPERTENSIVE PATIENTS IN SHENDI LOCALITY AT RIVER NILE STATE

2020 ◽  
Vol 8 (1) ◽  
pp. 18-25
Author(s):  
Alaa Ibrahim S. M. Khair ◽  
Mosab Khalid ◽  
Haghamad Allzain
Keyword(s):  
Diabetes Mellitus ◽  
Renal Function ◽  
Renal Dysfunction ◽  
Control Group ◽  
P Value ◽  
Case Group ◽  
River Nile ◽  
Control Groups ◽  
Hypertensive Patients ◽  
And Control

Microalbuminuria in urine is a known indicator of renal dysfunction. It has been extensively studied in the diabetes mellitus and hypertensive patients. This study was cross-sectional, descriptive case-control, hospital based, conducted, in Shendi locality at River Nile State in Northern Sudan, from March to July 2018.  It intended to examine the effect of diabetes mellitus and hypertension on renal function. Urine and venous blood samples were collected sixty (60) volunteer subjects, thirty-five (35) of them known as diabetic and hypertensive patients as case group, twenty-five (25) healthy subjects as control group. Samples were examined for urine microalbuminuria and plasma creatinine levels. Case and control groups were compared by age, gender and exercise. Albumin/Creatinine Ratio (ACR). was also assessed. The mean microalbuminuria and mean ACR were significantly higher in case group (38.304mg/dl, (6.8226) mg/mmol) compared to control group (13.020mg/dl, 2.4512) mg/mmol.), with statistically significant P. value (0.007) and (0.000) respectively.  There was statistically insignificant variation of the means of plasma creatinine between case and control groups (0.88, 0.948) mg/dl with P. value of (0.285). The present study concluded that diabetes and hypertension were associated with microalbuminuria and renal dysfunction.

Conjugated Linoleic Acid Causes a Marked Increase in Liver α-Tocopherol and Liver α-Tocopherol Transfer Protein in C57BL/6 J Mice

2010 ◽  
Vol 80 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Pei-Min Chao ◽  
Wan-Hsuan Chen ◽  
Chun-Huei Liao ◽  
Huey-Mei Shaw
Keyword(s):  
Antioxidant Enzymes ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substances ◽  
Control Groups ◽  
Protein Levels ◽  
Transfer Protein ◽  
And Control

Conjugated linoleic acid (CLA) is a collective term for the positional and geometric isomers of a conjugated diene of linoleic acid (C18:2, n-6). The aims of the present study were to evaluate whether levels of hepatic α-tocopherol, α-tocopherol transfer protein (α-TTP), and antioxidant enzymes in mice were affected by a CLA-supplemented diet. C57BL/6 J mice were divided into the CLA and control groups, which were fed, respectively, a 5 % fat diet with or without 1 g/100 g of CLA (1:1 mixture of cis-9, trans-11 and trans-10, cis-12) for four weeks. α-Tocopherol levels in plasma and liver were significantly higher in the CLA group than in the control group. Liver α-TTP levels were also significantly increased in the CLA group, the α-TTP/β-actin ratio being 2.5-fold higher than that in control mice (p<0.01). Thiobarbituric acid-reactive substances were significantly decreased in the CLA group (p<0.01). There were no significant differences between the two groups in levels of three antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase). The accumulation of liver α-tocopherol seen with the CLA diet can be attributed to the antioxidant potential of CLA and the ability of α-TTP induction. The lack of changes in antioxidant enzyme protein levels and the reduced lipid peroxidation in the liver of CLA mice are due to α-tocopherol accumulation.

Sri Lankan Cleft Lip and Palate Study Model Analysis: Clefts of the Secondary Palate

1993 ◽  
Vol 30 (2) ◽  
pp. 227-230 ◽  
Author(s):  
Andrew Mccance ◽  
David Roberts-Harry ◽  
Martyn Sherriff ◽  
Michael Mars ◽  
William J.B. Houston
Keyword(s):  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Model Analysis ◽  
Control Group ◽  
Tooth Size ◽  
Sri Lankan ◽  
Control Groups ◽  
Secondary Palate ◽  
Comparable Control ◽  
And Control

The study models of a group of adult Sri Lankan patients with clefts of the secondary palate were investigated. Tooth-size and arch-dimension comparisons were made with a comparable control group. Significant differences were found between the cleft and control groups in tooth sizes, chord lengths, and arch widths. The cleft group dimensions were generally smaller than those of the control group. Overjets were larger in the cleft group.

THE EFFECT OF APPLYING ANIMAL CARTOON PICTURES ON STUDENTS’ ACHIEVEMENT IN WRITING NARRATIVE TEXT

2017 ◽  
Vol 6 (1) ◽  
Author(s):  
Hasanul Arifin Zul And Masitowarni Siregar
Keyword(s):  
T Test ◽  
Narrative Text ◽  
Control Group ◽  
First Year ◽  
Writing Achievement ◽  
Control Groups ◽  
Post Test ◽  
Written Test ◽  
And Control ◽  
Experimental Group

This thesis is focused on the investigation of the effect of applying animal cartoon pictures on students’ achievement in writing narrative text. This study aims to find whether applying animal cartoon pictures significantly affect the students’ writing achievement or not. The data in this study were obtained by administering a written test. The population was the 2015/2016 first year (grade XI) of SMA Swasta Nusantara Lubuk Pakam and 66 students were taken as the sample by using random sampling. The sample was divided into two groups, experimental and control groups. The experimental group was taught by applying animal cartoon pictures while the control group without animal cartoon pictures (x = lecturing). The data were taken the scores from the pre-test and post-test to both experimental and control groups. These data were analyzed by using t-test. The result of computing the t-test obviously showed that t-observed is higher than t-table (5.21 >1,67) with the degree of freedom 64 (df =N-2) at the level significance 0,05 one tail test. It showed that the application of animal cartoon pictures significantly affected the students of SMA Swasta Nusantara Lubuk Pakam achievement in writing narrative text.

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